Antidiabetic activity of herbal green tea extract from white mangrove (Avicennia marina) leaves towards blood glucose level of diabetic wistar rats (Rattus novergicus)

Green tea Camellia sinensis contains polyphenol that has antidiabetic activity. Mangrove leaves also contain polyphenol which potentially gives these leave antidiabetic activity. The aim of this research was to determine the ability of herbal green tea extract from white mangrove (Avicennia marina) leaves to decrease blood glucose level of diabetic Wistar rats (Rattus novergicus). The method used was experimental and involved giving a herbal green tea extract from white mangrove leaves with concentration of 100, 200 and 300 mg/200g BW/day, and positive control, i.e. glybenclamid (0.09 mg/200 g BW/day), to diabetic rats injected with Streptozotocin (STZ) and Nicotinamide (NA). The rats were observed on day 0, 5, 10 and 15. The results showed that the herbal green tea extract from white mangrove leaves decreased the blood glucose level of diabetic rats. The effective extract dose that decreased the blood glucose level of diabetic rats was 300 mg/200 g BW, which is comparable to the effect produced by glybenclamid (antidiabetic medicine). This dose could decrease the blood glucose level of diabetic rats to reach a normal blood glucose level after 20 days.

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PENGENDALIAN KADAR GLUKOSA DARAH OLEH TEH HIJAU DAN ATAU TEH DAUN MURBEI PADA TIKUS DIABETES

Jurnal Gizi dan Pangan ◽

10.25182/jgp.2010.5.2.87-94 ◽

2010 ◽

Vol 5 (2) ◽

pp. 87

Author(s):

Rusman Efendi ◽

Evy Damayanthi ◽

Lilik Kustiyah ◽

Nastiti Kusumorini

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Green Tea ◽

Glucose Level ◽

Diabetic Rats ◽

Feed Consumption ◽

Font Size ◽

High Prevalence ◽

The Difference ◽

Control Diabetes

Diabetes mellitus is degeneratif disease with high prevalence that happens in many countries. Several studies had been done to control diabetes by using green tea, mullberry leaf tea, and their mixture. The aim of this research was to analyze the influence of the administration green tea, mullbery leaf tea, and their mixtures to blood glucose level of diabetic rats both during 120 minutes after administration. This research had four phases, first to determine the best mullberry leaf tea, second to fourth phases respectively, determine turnover of blood glucose level on normal rats; attempt during 120 minutes on diabetic rats. The result of research during 120 minutes have showed that blood glucose level on diabetic rats which were administered by green tea, mullberry leaf tea and their mixture is significantly difference with diabetic rats which were administered by water. Blood glucose level at baseline increased at 30th minutes and showed the difference significantly and then until 60th and 120th minutes and relatively stable. During 120 minutes after feed consumption, inhibition of blood glucose level occured increasingly on diabetic rats which were administered by green tea, mullberry leaf tea, and their mixture compared to diabetic rats which were administered by water.

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EVALUATION OF ANTIHYPERGLYCAEMIC EFFECT OF BUTEA MONOSPERMA LEAF EXTRACT ON ADRENALIN INDUCED AND HIGH GLUCOSE FEED ANIMAL MODEL

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i12.21501 ◽

2017 ◽

Vol 10 (12) ◽

pp. 301

Author(s):

Biswaranjan Ray ◽

Santosh Kumar Mahapatra ◽

Pradipta Kumar Behera ◽

Ashok K Panigrahi

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

High Glucose ◽

Ethanolic Extract ◽

Biologically Active ◽

Antidiabetic Activity ◽

Blood Levels ◽

Active Components ◽

Butea Monosperma

Objective: As per the ethnopharmacological information has Butea monosperma been used to treat diabetes mellitus by the tribal people of tropical and subtropical areas. However, there is no much more scientific report available about the antidiabetic property of the leaves of the plant. Hence, the study was undertaken to evaluate the antidiabetic effect of ethanolic extract of B. monosperma on blood levels of adrenaline-induced and glucose feed diabetic rabbits.Methods: The three different doses of the extracts (100, 200, and 400 mg/kg) were administered orally to an experimental animal. The animals were induced diabetics by adrenaline and high glucose diet. Blood glucose level was measured accordingly. For antidiabetic activity, photocolorimeter was used to monitoring the blood glucose level with crest kit box (GOP-POD method).Results: The extracts showed considerable dose-dependent activity. However, the dose 400 mg/kg showed considerable lower of blood glucose level. p<0.01 indicates the significance result. 8 hrs reading 182.5±3.83 for 400 mg does is most effective for reducing blood sugar.Conclusion: The study indicates that the ethanolic extract of B. monosperma leaves possesses antidiabetic properties which suggest the presence of biologically active components.

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Regulated Effects of Capsicum frutescens Supplemented Diet (C.F.S.D) on Fasting Blood Glucose Level, Biochemical Parameters and Body Weight in Alloxan Induced Diabetic Wistar Rats

British Journal of Pharmaceutical Research ◽

10.9734/bjpr/2013/3256 ◽

2013 ◽

Vol 3 (3) ◽

pp. 496-507 ◽

Cited By ~ 4

Author(s):

Ojieh Anthony

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Biochemical Parameters ◽

Wistar Rats ◽

Fasting Blood Glucose ◽

Fasting Blood Glucose Level ◽

Capsicum Frutescens

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Effects of Crude Extract of Neem Bark on the Pancreas of Streptozotocin Induced Diabetic Wistar Rats

Asian Journal of Research in Medical and Pharmaceutical Sciences ◽

10.9734/ajrimps/2019/v6i330100 ◽

2019 ◽

pp. 1-9

Author(s):

A. J. Ajibade ◽

E. O. James ◽

B. D. Kehinde

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Crude Extract ◽

Wistar Rats ◽

Enzymatic Activities ◽

Diabetic Group ◽

Bark Extract ◽

Group A ◽

Group B

This study investigated the histological and serum enzymatic activities of Azadirachta indica, an Indian medicinal plant, on the pancreas in streptozotocin induced diabetic adult wstar rats. Forty six adult wistar rats weighing 100 g to 220 g were randomly separated into four groups; Group A was regarded as the control , while group B was the diabetic group, C and diabetic – extract treated group. The control group received distilled water throughout the experiment; the remaining three groups were induced with streptozotocin intra-peritoneally to induced diabetes in the wistar rats. After some days, the animals were confirmed diabetic with the help of a measuring glucometer. Thereafter, group B diabetic rats remained untreated while Group C and D were treated with a low dose (250 mg/kg) of the crude neem bark extract and a high dose (500 mg/kg) of the extract respectively for 42 days. The aqueous neem bark extract was suspended in the drinking water of the treated animals for the period of 42 days. The body weights of the animals were weighed weekly and likewise the measurement of the blood glucose level was taken. The animals were sacrificed at the end of 42 days by cervical dislocation and the pancreas was removed and weighed immediately using sensitive weighing balance. The blood samples were collected from the sacrificed animals into EDTA bottle for serum enzymatic analysis. The organ pancreas was fixed in a 10% formol saline, processed and stained with Heamatoxylin and Eosin for general histological study. The microscopic examination of diabetic group showed some degenerative and necrotic cells which made the pancreatic tissue distorted compared with the control that appeared normal. The diabetic group C and D rats treated with the extract showed ameliorative potentials of the extract with improvement in histo-architecture of the pancreatic tissue following recovery from damage. Analysis of the blood serum level showed that the aqueous neem bark extract has ameliorative effect on the enzymatic activities in serum of the treated rats. The alanine amino transferase, aspartate amino transferase and alkaline phosphatase levels were significantly reduced (P<0.05) in the crude extract- treated animals compared with the untreated group B and control group A with significantly increased ( P<0.05 ) enzymatic activities in wistar rats in these groups. The antioxidant status was compromised in diabetic group B with significantly increased (P<0.05) LPO, reduced SOD and GSH compared with significantly reduced (P<0.05) LPO, increased (P<0.05) SOD and GSH in group A- control and Group C and D diabetic-extract treated Wistar rats. Similarly, the blood glucose level increased significantly (P<0.05) in group B diabetic group compared with significantly reduced (P<0.05) blood glucose level in group A control as well as C and D extract-treated rats. The study concluded that crude extract of neem has ameliorative potentials on streptozotocin-induced diabetic wistar rats characterized by oxidative damage which reveals improvement in tissue morphology.

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Extracts of Guiera senegalensis J.F. Gmel (Combretaceae) Galls Ameliorates Streptozotocin-Induced Diabetes Mellitus Rats Status

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2019/v25i230071 ◽

2019 ◽

pp. 1-11

Author(s):

Pierre Alexandre Eric Djifaby Sombié ◽

Rahman Hafizur ◽

Martin Kiendrébéogo ◽

Muhammad Iqbal Choudhary ◽

Odile Germaine Nacoulma

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Antioxidant Status ◽

Wistar Rats ◽

Methanol Extract ◽

Insulin Level ◽

Diabetic Rats ◽

Antidiabetic Activity ◽

Guiera Senegalensis

Background: A good number of medicinal and dietary plants are used for diabetes treatment in Burkina Faso. Aim of the Study: The present study aimed to investigate the antidiabetic activity of Guiera senegalensis galls extracts and its potential mechanisms in streptozotocin-induced diabetic rats. Methodology: The methanol extract was administered by gavage to healthy Wistar rats for the determination of toxicity, to normal and diabetic Wistar rats for the determination of glucose reduction level, lipid profile, insulin level and glycaemic parameters in serum. The histology and immunohistochemistry of the pancreas were also determined. Results: The acute toxicity results showed that the medium lethal dose (LD50) of the methanol galls extract of Guiera senegalensis is greater than 2000 mg/kg body weight in rats. Guiera senegalensis methanolic extract (250 mg/kg) and the tolbutamide (100 mg/kg) recorded a significantly (p < 0.05) lower level of triglyceride compared to the diabetic group. The methanol extract (250 and 500 mg/kg pc) signiﬁcantly (p < 0.05) decreased the blood glucose level and increased the serum insulin level in diabetic rats. Interestingly, improved ß-cell function and antioxidant status were also observed in G. senegalensis-treated diabetic rats when compared to tolbutamide-treated diabetic rats. Conclusion: These data showed direct evidence that G. senegalensis has antidiabetic activity by decreasing blood glucose level, improving insulin secretion and β-cell functions and modulating antioxidant status.

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Protective effect of Berberis asiatica root on biochemical and histopathological changes in streptozotocin-induced diabetic Wistar rats

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20173110 ◽

2017 ◽

Vol 6 (8) ◽

pp. 1880 ◽

Cited By ~ 2

Author(s):

Mohd Muddassir Husain Khan ◽

Chetan Rastogi ◽

Sachin Gupta ◽

Shravan Kumar Paswan ◽

Pritt Verma ◽

...

Keyword(s):

Blood Glucose ◽

Liver Function ◽

Blood Glucose Level ◽

Protective Effect ◽

Glucose Level ◽

Wistar Rats ◽

Total Bilirubin ◽

Group Iv ◽

Group Iii ◽

Group I

Background: This study was designed to evaluate the effect of Berberis asiatica root extract (BAE) against streptozotocin induced elevated blood glucose level and other liver and kidney functions changes in adult male Wistar rats.Methods: Thirty male Wistar rats were divided into five groups of six rats in each (Groups I-V). Group I and Group II served as normal control and disease control, respectively. Group III received standard anti-diabetic drug glibenclamide (5mg/kg), while Group IV and Group V received the low dose (250mg/kg) and high dose (500mg/kg) of BAE. Serum blood glucose, SGOT, SGPT, ALP, total bilirubin, BUN, serum creatinine, TC, TG, HDL-C, LDL-C, and VLDL-C were estimated using standard methods. After collection of samples for biochemical evaluation, the pancreas from each animal was isolated and examined for histological changes.Results: BAE and glibenclamide treated disease rats showed significant (p <0.05) decrease in blood glucose concentration. Treatment with BAE at 250mg/kg and 500mg/kg in Group IV and V and standard drug glibenclamide in Group III showed significant (p <0.05) reduction in the level of liver function substances such as SGOT, SGPT, ALP and total bilirubin as compared to disease group, as well as showed significant (p <0.05) decrease in renal panel. Liver function parameters were significantly (p <0.05) improved in groups treated with BAE. Histopathological analysis revealed the protective effect of BAE against streptozotocin induced damage to islets of Langerhans.Conclusions: This study showed the destruction of islets of Langerhans and elevation in blood glucose level as well as alteration in other biochemical parameters were ameliorated by the effect of Berberis asiatica extract.

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Effect of green tea extract (Camellia sinensis) on the spermatic parameters of Wistar rats submitted or not to testicular heat shock

Animal Reproduction ◽

10.1590/1984-3143-ar2019-0049 ◽

2020 ◽

Vol 17 (2) ◽

Author(s):

Joane Isis Travassos Vieira ◽

Taylane Alves da Silva ◽

Williane Maria Pereira Barbosa ◽

Gabriela Lima de Azevêdo ◽

Lúcia Cristina Pereira Arruda ◽

...

Keyword(s):

Heat Shock ◽

Green Tea ◽

Camellia Sinensis ◽

Wistar Rats ◽

Green Tea Extract ◽

Tea Extract

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Aktivitas Antihiperglikemik dari Ekstrak Etanol dan Heksana Tumbuhan Suruhan (Peperomia pellucida [L.] Kunth) pada Tikus Wistar (Rattus norvegicus L.) yang Hiperglikemik

Jurnal MIPA ◽

10.35799/jm.2.2.2013.2999 ◽

2013 ◽

Vol 2 (2) ◽

pp. 109

Author(s):

Sariyana Togubu ◽

Lidya I. Momuat ◽

Jessy E. Paendong ◽

Navila Salma

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Wistar Rats ◽

Ethanol Extract ◽

Tolerance Test ◽

Negative Control ◽

Hexane Extract ◽

Test Method

Telah dilakukan penelitian kadar glukosa darah tikus wistar yang hiperglikemia pada pemberian ekstrak etanol dan heksana tumbuhan suruhan (Peperomia pellucida [L]. Kunth). Penelitian ini menggunakan metode uji toleransi glukosa, terhadap 16 ekor tikus jantan wistar yang hiperglikemia akibat diinduksi sukrosa. Selanjutnya, tikus dibagi dalam 4 kelompok perlakuan yaitu kelompok kontrol negatif (K-) diberi CMC 0,5%, kelompok kontrol positif (K+) diberi Glibenklamid dosis 0,45 mg/kgBB, kelompok ekstrak etanol tumbuhan suruhan (EETS) dosis 40 mg/kgBB, dan kelompok ekstrak heksana tumbuhan suruhan (EHTS) dosis 40 mg/kgBB. Setiap kelompok terdiri dari 4 ekor tikus. Kadar glukosa darah tikus diukur dengan alat Drglukometer pada menit ke-30, 60, dan 120 setelah diberikan perlakuan. Hasil penelitian menunjukkan bahwa pada menit ke-120, kadar glukosa darah tikus pada kelompok EETS dan EHTS masing-masing turun sebesar 54,57% dan 51,25%, dan tidak berbeda nyata dengan K(+). Kadar glukosa darah kelompok K(+) dan EETS pada menit ke-120 mencapai kadar yang sama dengan keadaan basal (normal). Penelitian ini menyimpulkan bahwa ekstrak etanol dan heksana tumbuhan suruhan dengan dosis 40 mg/KgBB memiliki efek hipoglikemik pada tikus jantan wistar yang hiperglikemia.A study on blood glucose level in hyperglycemic wistar rats treated with ethanol and hexane extracts of suruhan (Peperomia pellucida [L]. Kunth) had been done. This study used glucose tolerance test method applied on 16 male wistar rats which were hyperglycemic by sucrose induction. The rats were divided into four treatment groups, each of which contained four rats, which were negative control (K-) group treated with CMC 0.5%, positive control (K+) group treated with glibenclamide 0.45 mg/kg body weight, group treated with ethanol extract of P. pellucida (EETS) 40 mg/kg body weight, and group treated with hexane extract of P. pellucida (EHTS) 40 mg/kg body weight. Glucose level in rat blood was measured using Drglucometer at minute 30, 60, and 120 after treatment. The results showed that at minute 120 the blood glucose level of EETS and EHTS was decreased by 54.57% and 51.25%, respectively, which were not significantly different from K(+). At minute 120, blood glucose of K(+) and EETS reached the same level with that of basal (normal) state. It was concluded that ethanol and hexane extract of P. pellucida 40 mg/kg body weight had hypoglycemic effect on hyperglycemic wistar rats.

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Evaluation of Antidiabetic Activity of a Novel Polyherbal Preparationagainst Streptozotocin Induced Diabetes Rat Model

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i26a31470 ◽

2021 ◽

pp. 42-54

Author(s):

K. Jyothsna Jayaraju ◽

B. Mohammed Ishaq

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Antidiabetic Activity ◽

Gymnema Sylvestre ◽

Standard Drug ◽

Glucose Levels ◽

Vinca Rosea ◽

Eugenia Jambolana ◽

Whole Plant

Background: Diabetes Mellitus is a chronic disorder characterised by abnormally elevated glucose levels in the blood. Diabetes is caused by one of two mechanisms: insufficient insulin synthesis (which is produced by the pancreas and reduces blood glucose) or insufficient response of cells to insulin action. The current aim of this research project was to formulate and evaluate the Polyherbal preparation (PHP) of the plants constituted with Cinnamonum zeylanicium (CJ) bark, Eugenia jambolana (EJ) seeds, Vinca rosea (VR) whole plant, Gymnema sylvestre (GS) leaves and determination of the anti-diabetic potential of the formulation in the animal model induced by Streptozotocin. Methods: Plant components in the current study used were Cinnamonum zeylanicium (CJ) bark, Eugenia jambolana (EJ) seeds, Vinca rosea (VR) whole plant, Gymnema sylvestre (GS) leaves were collected.Using a hydroalcoholic solvent, physico-chemical parameters and active chemical constituents were evaluated. The active components present in the extracts were identified by Preliminary phytochemical screening. The PHP acute toxicity analysis was conducted in compliance with OECD Guideline 423, with 200 mg/kg and 4000 mg/kg administered orally to rats over 28 days. Results: Diabetes was induced by STZ and treated with PHF did not show any alterations in behavior and no mortality was observed up to the 2000 mg/kg dose level during the interventional period. By oral administration of PHP with a dosage of 200 and 400 mg/kg, OGTT resulted in a steady decrease in blood glucose levels of 68.74±4.63 mg/dl and 63.83±1.74 mg/dl at 180min after the trial which proves that PHP possess anti-diabetic activity. By mixing each extract in varying proportions, PHP was developed and evaluated. PHP (200 and 400mg/kg) antidiabetic activity wasdetermined for streptozotocin (STZ)-induced diabetes in rats and glibenclamide (5.0mg/kg body weight) was used as a standard drug.The investigational drug was administered for 28 days and the blood glucose level effect of the PHP was analysed on the 28th day after the intervention time. Conclusion: The experimental study showed that a persistent and substantial decrease in the average blood glucose level of diabetic rats was observed with repeated administration of PHP and glibenclamide for 28 days. PHP demonstrated substantial antidiabetic and antihyperlipidemic activity similar to the standard drug. The formulation will emerge as a possible mixture that may challenge the synthetic drug.

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