scholarly journals Extraordinary centromeres: differences in the meiotic chromosomes of two rock lizards speciesDarevskia portschinskiiandDarevskia raddei

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6360 ◽  
Author(s):  
Victor Spangenberg ◽  
Marine Arakelyan ◽  
Eduard Galoyan ◽  
Mark Pankin ◽  
Ruzanna Petrosyan ◽  
...  
Keyword(s):  
Mitotic Chromosome ◽  
Parental Species ◽  
Primary Spermatocyte ◽  
Chromosome Analysis ◽  
Chromatin Modifications ◽  
Future Studies ◽  
Parthenogenetic Species ◽  
Meiotic Chromosomes ◽  
Species Specific ◽  
First Time

According to the synthesis of 30 years of multidisciplinary studies, parthenogenetic species of rock lizards of genusDarevskiawere formed as a result of different combination patterns of interspecific hybridization of the four bisexual parental species:Darevskia raddei,D. mixta,D. valentini, andD. portschinskii. In particular,D. portschinskiiandD. raddeiare considered as the parental species for the parthenogenetic speciesD. rostombekowi. Here for the first time, we present the result of comparative immunocytochemical study of primary spermatocyte nuclei spreads from the leptotene to diplotene stages of meiotic prophase I in two species:D. portschinskiiandD. raddei. We observed similar chromosome lengths for both synaptonemal complex (SC) karyotypes as well as a similar number of crossing over sites. However, unexpected differences in the number and distribution of anti-centromere antibody (ACA) foci were detected in the SC structure of bivalents of the two species. In all examinedD. portschinskiispermatocyte nuclei, one immunostained centromere focus was detected per SC bivalent. In contrast, in almost every studiedD. raddeinuclei we identified three to nine SCs with additional immunostained ACA foci per SC bivalent. Thus, the obtained results allow us to identify species-specific karyotype features, previously not been detected using conventional mitotic chromosome analysis. Presumably the additional centromere foci are result of epigenetic chromatin modifications. We assume that this characteristic of theD. raddeikaryotype could represent useful marker for the future studies of parthenogenetic species hybrid karyotypes related toD. raddei.

scholarly journals Extraordinary centromeres: differences in the meiotic chromosomes of two rock lizards species Darevskia portschinskii and Darevskia raddei

2018 ◽  
Author(s):  
Victor Spangenberg ◽  
Marine Arakelyan ◽  
Eduard Galoyan ◽  
Mark Pankin ◽  
Ruzanna Petrosyan ◽  
...  
Keyword(s):  
Mitotic Chromosome ◽  
Parental Species ◽  
Primary Spermatocyte ◽  
Chromosome Analysis ◽  
Chromatin Modifications ◽  
Future Studies ◽  
Parthenogenetic Species ◽  
Meiotic Chromosomes ◽  
Species Specific ◽  
First Time

According to the synthesis of 30 years of multidisciplinary studies parthenogenetic species of rock lizards of genus Darevskia were formed as a result of different combination patterns of interspecific hybridization of the four bisexual parental species: D. raddei, D. mixta, D. valentini, and D. portschinskii. In particular D. portschinskii and D. raddei are considered as the parental species for the parthenogenetic species D. rostombekowi. Here for the first time we present the result of comparative immunocytochemical study of primary spermatocyte nuclei spreads from the leptotene to diplotene stages of meiotic prophase I in two species: D. portschinskii and D. raddei. We observed similar chromosome lengths for both synaptonemal complex (SC) karyotypes as well as similar number of crossing over sites. However, unexpected differences in the number and distribution of anti-centromere antibody (ACA) foci were detected in the SC structure of bivalents of the two species. In all examined D. portschinskii spermatocyte nuclei, one immunostained centromere focus was detected per SC bivalent. In contrast, in almost every studied D. raddei nuclei we identified three – nine SCs with additional immunostained ACA foci per SC bivalent. Thus, the obtained results allow us to identify species-specific karyotype features, previously not been detected using conventional mitotic chromosome analysis. Presumably the additional centromere foci are result of epigenetic chromatin modifications. We assume that this characteristic of the D. raddei karyotype could represent useful marker for the future studies of parthenogenetic species hybrid karyotypes related to D. raddei.

scholarly journals Extraordinary centromeres: differences in the meiotic chromosomes of two rock lizards species Darevskia portschinskii and Darevskia raddei

2018 ◽  
Author(s):  
Victor Spangenberg ◽  
Marine Arakelyan ◽  
Eduard Galoyan ◽  
Mark Pankin ◽  
Ruzanna Petrosyan ◽  
...  
Keyword(s):  
Mitotic Chromosome ◽  
Parental Species ◽  
Primary Spermatocyte ◽  
Chromosome Analysis ◽  
Chromatin Modifications ◽  
Future Studies ◽  
Parthenogenetic Species ◽  
Meiotic Chromosomes ◽  
Species Specific ◽  
First Time

According to the synthesis of 30 years of multidisciplinary studies parthenogenetic species of rock lizards of genus Darevskia were formed as a result of different combination patterns of interspecific hybridization of the four bisexual parental species: D. raddei, D. mixta, D. valentini, and D. portschinskii. In particular D. portschinskii and D. raddei are considered as the parental species for the parthenogenetic species D. rostombekowi. Here for the first time we present the result of comparative immunocytochemical study of primary spermatocyte nuclei spreads from the leptotene to diplotene stages of meiotic prophase I in two species: D. portschinskii and D. raddei. We observed similar chromosome lengths for both synaptonemal complex (SC) karyotypes as well as similar number of crossing over sites. However, unexpected differences in the number and distribution of anti-centromere antibody (ACA) foci were detected in the SC structure of bivalents of the two species. In all examined D. portschinskii spermatocyte nuclei, one immunostained centromere focus was detected per SC bivalent. In contrast, in almost every studied D. raddei nuclei we identified three – nine SCs with additional immunostained ACA foci per SC bivalent. Thus, the obtained results allow us to identify species-specific karyotype features, previously not been detected using conventional mitotic chromosome analysis. Presumably the additional centromere foci are result of epigenetic chromatin modifications. We assume that this characteristic of the D. raddei karyotype could represent useful marker for the future studies of parthenogenetic species hybrid karyotypes related to D. raddei.

scholarly journals Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Maroua Oueslati ◽  
Magdalena Mulet ◽  
Mohamed Zouaoui ◽  
Charlotte Chandeysson ◽  
Jorge Lalucat ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Pseudomonas Syringae ◽  
Ice Nucleation ◽  
Phylogenetic Study ◽  
Future Studies ◽  
Citrus Orchards ◽  
First Time ◽  
New Cultivars

Abstract The damages observed in Tunisian citrus orchards have prompted studies on the Pseudomonas spp. responsible for blast and black pit. Prospective orchards between 2015 and 2017 showed that the diseases rapidly spread geographically and to new cultivars. A screening of Pseudomonas spp. isolated from symptomatic trees revealed their wide diversity according to phylogenetic analysis of their housekeeping rpoD and cts genes. The majority of strains were affiliated to Pseudomonas syringae pv. syringae (Phylogroup PG02b), previously described in Tunisia. However, they exhibited various BOX-PCR fingerprints and were not clonal. This work demonstrated, for the first time in Tunisia, the involvement of Pseudomonas cerasi (PG02a) and Pseudomonas congelans (PG02c). The latter did not show significant pathogenicity on citrus, but was pathogenic on cantaloupe and active for ice nucleation that could play a role in the disease. A comparative phylogenetic study of citrus pathogens from Iran, Montenegro and Tunisia revealed that P. syringae (PG02b) strains are closely related but again not clonal. Interestingly P. cerasi (PG02a) was isolated in two countries and seems to outspread. However, its role in the diseases is not fully understood and it should be monitored in future studies. The diversity of pathogenic Pseudomonas spp. and the extension of the diseases highlight that they have become complex and synergistic. It opens questions about which factors favor diseases and how to fight against them efficiently and with sustainable means.

scholarly journals Bark traits, decomposition and flammability of Australian forest trees

2017 ◽  
Vol 65 (4) ◽  
pp. 327 ◽  
Author(s):  
Saskia Grootemaat ◽  
Ian J. Wright ◽  
Peter M. van Bodegom ◽  
Johannes H. C. Cornelissen ◽  
Veronica Shaw
Keyword(s):  
Forest Tree ◽  
Remarkable Feature ◽  
Interspecific Differences ◽  
Eucalypt Plantations ◽  
Physical Traits ◽  
Key Driver ◽  
Multiple Species ◽  
Species Specific ◽  
First Time ◽  
Different Tissues

Bark shedding is a remarkable feature of Australian trees, yet relatively little is known about interspecific differences in bark decomposability and flammability, or what chemical or physical traits drive variation in these properties. We measured the decomposition rate and flammability (ignitibility, sustainability and combustibility) of bark from 10 common forest tree species, and quantified correlations with potentially important traits. We compared our findings to those for leaf litter, asking whether the same traits drive flammability and decomposition in different tissues, and whether process rates are correlated across tissue types. Considerable variation in bark decomposability and flammability was found both within and across species. Bark decomposed more slowly than leaves, but in both tissues lignin concentration was a key driver. Bark took longer to ignite than leaves, and had longer mass-specific flame durations. Variation in flammability parameters was driven by different traits in the different tissues. Decomposability and flammability were each unrelated, when comparing between the different tissue types. For example, species with fast-decomposing leaves did not necessarily have fast-decomposing bark. For the first time, we show how patterns of variation in decomposability and flammability of bark diverge across multiple species. By taking species-specific bark traits into consideration there is potential to make better estimates of wildfire risks and carbon loss dynamics. This can lead to better informed management decisions for Australian forests, and eucalypt plantations, worldwide.

scholarly journals The Morphological and Anatomical Traits of the Leaf in Representative Vinca Species Observed on Indoor- and Outdoor-Grown Plants

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 622
Author(s):  
Alexandra Ciorîță ◽  
Septimiu Cassian Tripon ◽  
Ioan Gabriel Mircea ◽  
Dorina Podar ◽  
Lucian Barbu-Tudoran ◽  
...  
Keyword(s):  
Spongy Parenchyma ◽  
Palisade And Spongy Parenchyma ◽  
Chemical Composition Of Plants ◽  
Ultrastructural Characteristics ◽  
Species Specific ◽  
Microscopy Techniques ◽  
First Time ◽  
Thick Layers ◽  
Space Ratio ◽  
Indoor And Outdoor

Morphological and anatomical traits of the Vinca leaf were examined using microscopy techniques. Outdoor Vinca minor and V. herbacea plants and greenhouse cultivated V. major and V. major var. variegata plants had interspecific variations. All Vinca species leaves are hypostomatic. However, except for V. minor leaf, few stomata were also present on the upper epidermis. V. minor leaf had the highest stomatal index and V. major had the lowest, while the distribution of trichomes on the upper epidermis was species-specific. Differentiated palisade and spongy parenchyma tissues were present in all Vinca species’ leaves. However, V. minor and V. herbacea leaves had a more organized anatomical aspect, compared to V. major and V. major var. variegata leaves. Additionally, as a novelty, the cellular to intercellular space ratio of the Vinca leaf’s mesophyll was revealed herein with the help of computational analysis. Lipid droplets of different sizes and aspects were localized in the spongy parenchyma cells. Ultrastructural characteristics of the cuticle and its epicuticular waxes were described for the first time. Moreover, thick layers of cutin seemed to be characteristic of the outdoor plants only. This could be an adaptation to the unpredictable environmental conditions, but nevertheless, it might influence the chemical composition of plants.

Intergeneric hybrids between Thinopyrum and Psathyrostachys (Triticeae)

Genome ◽  
1990 ◽  
Vol 33 (6) ◽  
pp. 845-849 ◽  
Author(s):  
Richard R.-C. Wang
Keyword(s):  
Genome Analysis ◽  
Parental Species ◽  
Intergeneric Hybrids ◽  
Meiotic Pairing ◽  
Green Leaves ◽  
Psathyrostachys Juncea ◽  
Basic Genome ◽  
Chromosome Associations ◽  
Thinopyrum Elongatum ◽  
First Time

Intergeneric hybrids were synthesized for the first time from the diploid crosses Thinopyrum elongatum (JeJe) × Psathyrostachys juncea (NjNj), T. elongatum × P. fragilis (NfNf), T. bessarabicum (JbJb) × P. huashanica (NhNh), and T. bessarabicum × P. juncea, as well as from a cross between the amphidiploid of T. bessarabicum × T. elongatum (JbJbJeJe) and P. juncea. Spikes of these hybrids are morphologically intermediate between those of the parental species. Double spikelets occurred occasionally at central nodes of the spikes. Glaucous blue leaves appeared in the F1 only in the cross T. bessarabicum × P. huashanica, suggesting that the gene(s) for glaucous blue leaves in T. bessarabicum is (are) recessive to a gene(s) for green leaves in P. juncea but is (are) dominant to that for yellowish green leaves in P. huashanica. Meiotic pairing at metaphase I in these diploid (JN) and triploid (JJN) hybrids revealed a very low level of homology between the basic J and N genome. Therefore, the J and N genomes are nonhomologous and justifiably represented by different genome symbols. The triploid hybrids exhibited a pattern of chromosome associations that substantiated the earlier conclusion that the genomes in T. bessarabicum and T. elongatum are two versions of a basic genome (J). These hybrids will be useful in genome analysis, forming new Leymus species with the J and N genomes and broadening the diversity in the genus Pascopyrum with the SHJN genomes.Key words: hybrid, Thinopyrum, Psathyrostachys, genome.

scholarly journals Ecological Observations on Hybrid Populations of European Plethodontid Salamanders, Genus Speleomantes

Diversity ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 285
Author(s):  
Enrico Lunghi ◽  
Fabio Cianferoni ◽  
Stefano Merilli ◽  
Yahui Zhao ◽  
Raoul Manenti ◽  
...  
Keyword(s):  
Body Condition ◽  
Parental Species ◽  
Stomach Contents ◽  
Trophic Niche ◽  
Natural Hybrid ◽  
Ecological Information ◽  
Total Length ◽  
Plethodontid Salamanders ◽  
The Future ◽  
First Time

Speleomantes are the only plethodontid salamanders present in Europe. Multiple studies have been performed to investigate the trophic niche of the eight Speleomantes species, but none of these studies included hybrid populations. For the first time, we studied the trophic niche of five Speleomantes hybrid populations. Each population was surveyed twice in 2020, and stomach flushing was performed on each captured salamander; stomach flushing is a harmless technique that allows stomach contents to be inspected. We also assessed the potential divergence in size and body condition between natural and introduced hybrids, and their parental species. Previously collected data on Speleomantes were included to increase the robustness of these analyses. In only 33 out of 134 sampled hybrid Speleomantes we recognized 81 items belonging to 11 prey categories. The frequency of empty stomachs was higher in females and individuals from natural hybrid populations, whereas the largest number of prey was consumed by males. We compared the total length and body condition of 685 adult salamanders belonging to three types of hybrids and three parental (sub)species. Three group of salamanders (one hybrid and two parental species) showed significantly larger size, whereas no difference in body condition was observed. This study provided novel ecological information on Speleomantes hybrid populations. We also provided insights into the potential divergence between hybrids and parental species in terms of size and body condition. We discuss our findings, and formulate several hypotheses that should be tested in the future.

Polytene and mitotic chromosome analysis in Ceratitis capitata (Diptera; Tephritidae)

1986 ◽  
Vol 28 (2) ◽  
pp. 180-188 ◽  
Author(s):  
D. G. Bedo
Keyword(s):  
X Chromosome ◽  
Silver Staining ◽  
Mitotic Chromosome ◽  
Ceratitis Capitata ◽  
Fat Body ◽  
Polytene Chromosomes ◽  
Chromosome Analysis ◽  
Mitotic Chromosomes ◽  
Chromosome Separation ◽  
Ectopic Pairing

Polytene chromosomes were found in several larval and pupal tissues of the Medfly, Ceratitis capitata, during a search for chromosomes suitable for detailed cytological analysis. Well-banded highly polytene chromosomes, which could be adequately separated and spread, were found in trichogen cells of the spatulate superior orbital bristles of male pupae. These chromosomes proved suitable for full polytene analysis. Thoracic trichogen cells of both male and female pupae also contain useful polytene chromosomes, although they are considerably thinner and thus more difficult to analyze. Contrasting with those in pupal trichogen cells, the chromosomes in the salivary glands, Malphighian tubules, midgut, hindgut, and fat body of larvae and pupae were difficult to prepare because of high levels of ectopic pairing and chromosome fragmentation. In hindgut preparations partial separation of up to three chromosomes was achieved, but in all other tissues no useful chromosome separation was possible. In trichogen polytene cells, five banded chromosomes and a prominent heterochromatic network associated with a nucleolus are found. The mitotic chromosomes respond to C- and Q-banding and silver staining with considerable variation. This is especially so in the X chromosome, which displays an extensive array of bands following both Q-banding and silver staining. Comparison of Q-banded metaphase and polytene chromosomes demonstrates that the five autosomes are represented by conventional polytene chromosomes, while the sex chromosomes are contained in the heterochromatic net, most of which fluoresces strongly. This suggests that the Q-bands of the mitotic X chromosome are replicated to a greater extent than the nonfluorescent material in polytene cells. This investigation shows C. capitata to have excellent cytological material for both polytene and mitotic analysis.Key words: Ceratitis capitata, Medfly, chromosomes (polytene), banding (chromosome).

scholarly journals Molecular and morphological approaches for species delimitation and hybridization investigations of two Cichla species

2017 ◽  
Vol 107 (0) ◽  
Author(s):  
Andrea A. F. Mourão ◽  
Diogo Freitas-Souza ◽  
Diogo T. Hashimoto ◽  
Daniela C. Ferreira ◽  
Fernanda D. do Prado ◽  
...  
Keyword(s):  
Species Delimitation ◽  
Parental Species ◽  
Future Research ◽  
Correct Identification ◽  
Pcr Multiplex ◽  
Pcr Rflp ◽  
Genetic Pattern ◽  
Morphometric Methods ◽  
Species Specific ◽  
Specific Nucleotide

ABSTRACT The hybridization is a widely-discussed issue in several studies with fish species. For some authors, hybridization may be related with diversification and speciation of several groups, or also with the extinction of populations or species. Difficulties to differentiate species and hybrids may be a problem to correctly apply a management of wild species, because hybrid lineages, especially the advanced ones, may resemble the parental species. The genus Cichla Bloch & Schneider, 1801 constitutes an interesting experimental model, considering that hybridization and taxonomic uncertainties hinder a correct identification. Considering these problems, in this study, we developed genetic methodologies and applied meristic and morphometric approaches in wild samples in order to identify species and for test a possible hybridization between Cichla kelberi Kullander & Ferreira, 2006 and Cichla piquiti Kullander & Ferreira, 2006. For this, C. kelberi, C. piquiti and potential hybrid ( carijó) individuals were collected in Paraná and Tietê rivers (SP, Brazil). For meristic and morphometric methods, the individuals were analyzed using the statistical software Pcord 5:31, while for molecular methods, primers for PCR-multiplex were designed and enzyme for PCR-RFLP were selected, under the species-specific nucleotide. All results indicated that the carijó is not an interspecific hybrid, because it presented identical genetic pattern and morphology closed to C. piquiti. Thus, we propose that carijó is a C. piquiti morphotype. In addition, this study promotes a new molecular tool that could be used in future research, monitoring and management programs of the genus Cichla.

scholarly journals Metagenomic Comparisons between Soft and Hard Feces of Plateau Pikas (Ochotona curzoniae)

Animals ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 149
Author(s):  
Haibo Fu ◽  
Wenjing Li
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
16S Rdna ◽  
Microbial Metabolism ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Future Studies ◽  
Ochotona Curzoniae ◽  
The Future ◽  
First Time

The division of hard and soft feces is an effective digestion strategy in the order Lagomorpha. Although previous studies have reported that hard and soft feces differ in morphology and component, the discrepancy in the microbiome remains unclear. This study explored the microbiomes of hard and soft feces in plateau pikas by sequencing the V3 and V4 regions of 16S rDNA. We found that hard feces harbored higher Firmicutes, while soft feces harbored higher Akkermansia. Increased rare bacterial taxa were observed in hard feces compared with soft feces. Moreover, hard and soft feces displayed a greater difference in terms of core operational taxonomy units (OTUs) compared to the total OTUs. The soft feces showed enhancements in all predicted Kyoto Encyclopedia of Genes and Genomes (KEGG) functions, indicating an advancing microbial metabolism compared to hard feces. The significantly upregulated pathways in soft feces were mainly enriched in metabolism of energy and carbohydrate, glycan biosynthesis, cofactors and vitamins, and amino acids—all of which are associated with increased contents of microbial proteins, vitamins, and short-chain fatty acids. Our study reports, for the first time, the differential microbiomes between hard and soft feces of pikas and provides direction for the future studies on cecotrophy.

Sign in / Sign up

Export Citation Format

Share Document