Cover cropping can be a stronger determinant than host crop identity for arbuscular mycorrhizal fungal communities colonizing maize and soybean

BackgroundUnderstanding the role of communities of arbuscular mycorrhizal fungi (AMF) in agricultural systems is imperative for enhancing crop production. The key variables influencing change in AMF communities are the type of cover crop species or the type of subsequent host crop species. However, how maize and soybean performance is related to the diversity of AMF communities in cover cropping systems remains unclear. We therefore investigated which cover cropping or host identity is the most important factor in shaping AMF community structure in subsequent crop roots using an Illumina Miseq platform amplicon sequencing.MethodsIn this study, we established three cover crop systems (Italian ryegrass, hairy vetch, and brown mustard) or bare fallow prior to planting maize and soybean as cash crops. After cover cropping, we divided the cover crop experimental plots into two subsequent crop plots (maize and soybean) to understand which cover cropping or host crop identity is an important factor for determining the AMF communities and diversity both in maize and soybeans.ResultsWe found that most of the operational taxonomic units (OTUs) in root samples were common in both maize and soybean, and the proportion of common generalists in this experiment for maize and soybean roots was 79.5% according to the multinomial species classification method (CLAM test). The proportion of OTUs specifically detected in only maize and soybean was 9.6% and 10.8%, respectively. Additionally, the cover cropping noticeably altered the AMF community structure in the maize and soybean roots. However, the differentiation of AMF communities between maize and soybean was not significantly different.DiscussionOur results suggest cover cropping prior to planting maize and soybean may be a strong factor for shaping AMF community structure in subsequent maize and soybean roots rather than two host crop identities. Additionally, we could not determine the suitable rotational combination for cover crops and subsequent maize and soybean crops to improve the diversity of the AMF communities in their roots. However, our findings may have implications for understanding suitable rotational combinations between cover crops and subsequent cash crops and further research should investigate in-depth the benefit of AMF on cash crop performances in cover crop rotational systems.

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Can phosphorus application and cover cropping alter arbuscular mycorrhizal fungal communities and soybean performance after a five-year phosphorus-unfertilized crop rotational system?

PeerJ ◽

10.7717/peerj.4606 ◽

2018 ◽

Vol 6 ◽

pp. e4606 ◽

Cited By ~ 9

Author(s):

Masao Higo ◽

Ryohei Sato ◽

Ayu Serizawa ◽

Yuichi Takahashi ◽

Kento Gunji ◽

...

Keyword(s):

Cover Crop ◽

Arbuscular Mycorrhizal ◽

P Uptake ◽

Flowering Stage ◽

P Nutrition ◽

Cover Cropping ◽

Amf Diversity ◽

P Application ◽

Soybean Roots ◽

Amf Communities

BackgroundUnderstanding diversity of arbuscular mycorrhizal fungi (AMF) is important for optimizing their role for phosphorus (P) nutrition of soybeans (Glycine max(L.) Merr.) in P-limited soils. However, it is not clear how soybean growth and P nutrition is related to AMF colonization and diversity of AMF communities in a continuous P-unfertilized cover cropping system. Thus, we investigated the impact of P-application and cover cropping on the interaction among AMF colonization, AMF diversity in soybean roots, soybean growth and P nutrition under a five-year P-unfertilized crop rotation.MethodsIn this study, we established three cover crop systems (wheat, red clover and oilseed rape) or bare fallow in rotation with soybean. The P-application rates before the seeding of soybeans were 52.5 and 157.5 kg ha−1in 2014 and 2015, respectively. We measured AMF colonization in soybean roots, soybean growth parameters such as aboveground plant biomass, P uptake at the flowering stage and grain yields at the maturity stage in both years. AMF community structure in soybean roots was characterized by specific amplification of small subunit rDNA.ResultsThe increase in the root colonization at the flowering stage was small as a result of P-application. Cover cropping did not affect the aboveground biomass and P uptake of soybean in both years, but the P-application had positive effects on the soybean performance such as plant P uptake, biomass and grain yield in 2015. AMF communities colonizing soybean roots were also significantly influenced by P-application throughout the two years. Moreover, the diversity of AMF communities in roots was significantly influenced by P-application and cover cropping in both years, and was positively correlated with the soybean biomass, P uptake and grain yield throughout the two years.DiscussionOur results indicated that P-application rather than cover cropping may be a key factor for improving soybean growth performance with respect to AMF diversity in P-limited cover cropping systems. Additionally, AMF diversity in roots can potentially contribute to soybean P nutrition even in the P-fertilized cover crop rotational system. Therefore, further investigation into the interaction of AMF diversity, P-application and cover cropping is required for the development of more effective P management practices on soybean growth performance.

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Cover-Crop Species as Distinct Biotic Filters in Weed Community Assembly

Weed Science ◽

10.1614/ws-d-14-00071.1 ◽

2015 ◽

Vol 63 (1) ◽

pp. 282-295 ◽

Cited By ~ 20

Author(s):

Richard G. Smith ◽

Lesley W. Atwood ◽

Fredric W. Pollnac ◽

Nicholas D. Warren

Keyword(s):

Cover Crops ◽

Community Assembly ◽

Cover Crop ◽

Weed Species ◽

Seeding Rate ◽

Crop Species ◽

Cover Cropping ◽

Directional Filtering ◽

Weed Communities ◽

Sorghum Sudangrass

Cover crops represent a potentially important biological filter during weed community assembly in agroecosystems. This filtering could be considered directional if different cover-crop species result in weed communities with predictably different species composition. We examined the following four questions related to the potential filtering effects of cover crops in a field experiment involving five cover crops grown in monoculture and mixture: (1) Do cover crops differ in their effect on weed community composition? (2) Is competition more intense between cover crops and weeds that are in the same family or functional group? (3) Is competition more intense across weed functional types in a cover-crop mixture compared with cover crops grown in monocultures? (4) Within a cover-crop mixture, is a higher seeding rate associated with more effective biotic filtering of the weed community? We found some evidence that cover crops differentially filtered weed communities and that at least some of these filtering effects were due to differential biomass production across cover-crop species. Monocultures of buckwheat and sorghum–sudangrass reduced the number of weed species relative to the no-cover-crop control by an average of 36 and 59% (buckwheat) and 25 and 40% (sorghum–sudangrass) in 2011 and 2012, respectively. We found little evidence that competition intensity was dependent upon the family or functional classification of the cover crop or weeds, or that cover-crop mixtures were stronger assembly filters than the most effective monocultures. Although our results do not suggest that annual cover crops exert strong directional filtering during weed community assembly, our methodological framework for detecting such effects could be applied to similar future studies that incorporate a greater number of cover-crop species and are conducted under a greater range of cover-cropping conditions.

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Organic zero-till in the northern US Great Plains Region: Opportunities and obstacles

Renewable Agriculture and Food Systems ◽

10.1017/s174217051100041x ◽

2011 ◽

Vol 27 (1) ◽

pp. 12-20 ◽

Cited By ~ 25

Author(s):

Patrick M. Carr ◽

Randy L. Anderson ◽

Yvonne E. Lawley ◽

Perry R. Miller ◽

Steve F. Zwinger

Keyword(s):

Crop Rotation ◽

Great Plains ◽

Cover Crops ◽

Cover Crop ◽

Growing Season ◽

Northern Great Plains ◽

Crop Species ◽

Cash Crops ◽

No Till ◽

The Us

AbstractThe use of killed cover crop mulch for weed suppression, soil erosion prevention and many other soil and crop benefits has been demonstrated in organic no-till or zero-till farming systems in eastern US regions and in Canada. Implements have been developed to make this system possible by terminating cover crops mechanically with little, if any, soil disturbance. Ongoing research in the US northern Great Plains is being conducted to identify cover crop species and termination methods for use in organic zero-till (OZ) systems that are adapted to the crop rotations and climate of this semi-arid region. Current termination strategies must be improved so that cover crop species are killed consistently and early enough in the growing season so that subsequent cash crops can be grown and harvested successfully. Delaying termination until advanced growth stages improves killing efficacy of cover crops and may provide weed-suppressive mulch for the remainder of the growing season, allowing no-till spring seeding of cash crops during the next growing season. Excessive water use by cover crops, inability of legume cover crops to supply adequate amounts of N for subsequent cash crops and failure of cover crops to suppress perennial weeds are additional obstacles that must be overcome before the use of killed cover crop mulch can be promoted as a weed control alternative to tillage in the US northern Great Plains. Use of vegetative mulch produced by killed cover crops will not be a panacea for the weed control challenges faced by organic growers, but rather one tool along with crop rotation, novel grazing strategies, the judicious use of high-residue cultivation equipment, such as the blade plow, and the use of approved herbicides with systemic activity in some instances, to provide organic farmers with new opportunities to incorporate OZ practices into their cropping systems. Emerging crop rotation designs for organic no-till systems may provide for more efficient use of nutrient and water resources, opportunities for livestock grazing before, during or after cash crop phases and improved integrated weed management strategies on organic farms.

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Interaction of Inherited Microbiota from Cover Crops with Cash Crops

Agronomy ◽

10.3390/agronomy11112199 ◽

2021 ◽

Vol 11 (11) ◽

pp. 2199

Author(s):

Kelly Ulcuango ◽

Mariela Navas ◽

Nelly Centurión ◽

Miguel Á. Ibañez ◽

Chiquinquirá Hontoria ◽

...

Keyword(s):

Community Structure ◽

Cover Crops ◽

Microbial Interactions ◽

Legacy Effects ◽

Soil Microbiota ◽

Soil Microbial ◽

Cash Crops ◽

Microbial Responses ◽

Subsequent Crop ◽

Macro And Micronutrients

Cover crops (CC) provide important ecosystem services that are demanded to achieve more sustainable agrosystems. However, the legacy effects of CC on the microbial community structure and its interactions with the subsequent cash crops (CaC) are still poorly understood, especially when CC mixtures are involved. In this work, five CC (3 monocultures and 2 mixtures) were selected in an experiment under semi-controlled conditions to investigate if CC monocultures and mixtures differed in their effects on soil and crop variables and if the identity of the subsequent crop modulates these effects. The two most consumed crops worldwide, wheat and maize, were sown separately after CC. The legacy effects of CC on the studied microbial variables largely depended on the interaction with the CaC. The vetch and the barley-vetch mixture stood out by providing the microbial conditions that enhanced the absorption of macro- and micronutrients, to finally seek the highest wheat biomass (>80% more than the control). In maize, the effects of CC on soil microbiota were more limited. The soil microbial responses for CC mixtures were complex and contrasting. In wheat, the barley-vetch mixture behaved like barley monoculture, whereas in maize, this mixture behaved like vetch monoculture. In both CaC, the barley-melilotus mixture differed completely from its monocultures, mainly through changes in archaea, Glomeromycota, and F:B ratio. Therefore, it is necessary to deepen the knowledge on the CC-CaC-microbial interactions to select the CC that most enhance the sustainability and yield of each agrosystem.

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Redistribution of phosphorus in soil through cover crop roots

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132004000300007 ◽

2004 ◽

Vol 47 (3) ◽

pp. 381-386 ◽

Cited By ~ 13

Author(s):

Júlio C. Franchini ◽

Marcos A. Pavan ◽

Mário Miyazawa

Keyword(s):

Pisum Sativum ◽

Cover Crops ◽

Cover Crop ◽

Lupinus Albus ◽

Growth Period ◽

Soil Surface ◽

Phosphate Fertilizer ◽

Vicia Sativa ◽

Crop Species ◽

Soil P

The objective of this study was to evaluate if cover crops can absorb P from the upper layers and transport it in their roots to subsoil layers. Samples of an Oxisol were placed in PVC columns. Super phosphate fertilizer was applied to the 0-10 cm soil surface layers. The cover crops tested were: Avena strigosa, Avena sativa, Secale cereale, Pisum sativum subsp arvense, Pisum sativum, Vicia villosa, Vicia sativa, Lupinus angustifoliu, Lupinus albus, and Triticum aestivum. After a growth period of 80 days the cover crop shoots were cut off and the soil was divided into 10cm layers and the roots of each layer were washed out. The roots and shoots were analyzed separated for total P contribution to the soil. Considerable amount of P was present in the roots of cover crops. Vicia sativa contained more than 60% of total plant P in the roots. The contribution of Vicia sativa to soil P bellow the fertilized zone was about 7 kg ha-1. It thus appeared that there existed a possibility of P redistribution into the soil under no tillage by using cover crops in rotation with cash crops. Vicia sativa was the most efficient cover crop species as P carrier into the roots from superficial layer to lower layers.

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Mustard Cover Crop Growth and Weed Suppression in Organic, Strawberry Furrows in California

HortScience ◽

10.21273/hortsci12576-17 ◽

2018 ◽

Vol 53 (4) ◽

pp. 432-440 ◽

Cited By ~ 1

Author(s):

Eric B. Brennan ◽

Richard F. Smith

Keyword(s):

Cover Crops ◽

Cover Crop ◽

Sinapis Alba ◽

Weed Suppression ◽

Shoot Biomass ◽

Plastic Mulch ◽

Seeding Rate ◽

Crop Cover ◽

Cover Cropping ◽

Growing Seasons

Strawberry (Fragaria ×ananassa Duch.) production in California uses plastic mulch–covered beds that provide many benefits such as moisture conservation and weed control. Unfortunately, the mulch can also cause environmental problems by increasing runoff and soil erosion and reducing groundwater recharge. Planting cover crops in bare furrows between the plastic cover beds can help minimize these problems. Furrow cover cropping was evaluated during two growing seasons in organic strawberries in Salinas, CA, using a mustard (Sinapis alba L.) cover crop planted at two seeding rates (1× and 3×). Mustard was planted in November or December after strawberry transplanting and it resulted in average densities per meter of furrow of 54 and 162 mustard plants for the 1× and 3× rates, respectively. The mustard was mowed in February before it shaded the strawberry plants. Increasing the seeding rate increased mustard shoot biomass and height, and reduced the concentration of P in the mustard shoots. Compared with furrows with no cover crop, cover-cropped furrows reduced weed biomass by 29% and 40% in the 1× and 3× seeding rates, respectively, although weeds still accounted for at least 28% of the furrow biomass in the cover-cropped furrows. These results show that growing mustard cover crops in furrows without irrigating the furrows worked well even during years with relatively minimal precipitation. We conclude that 1) mustard densities of ≈150 plants/m furrow will likely provide the most benefits due to greater biomass production, N scavenging, and weed suppression; 2) mowing was an effective way to kill the mustard; and 3) high seeding rates of mustard alone are insufficient to provide adequate weed suppression in strawberry furrows.

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Systemic Colonization by Metarhizium robertsii Enhances Cover Crop Growth

Journal of Fungi ◽

10.3390/jof6020064 ◽

2020 ◽

Vol 6 (2) ◽

pp. 64

Author(s):

Imtiaz Ahmad ◽

María del Mar Jiménez-Gasco ◽

Dawn S. Luthe ◽

Mary E. Barbercheck

Keyword(s):

Cover Crops ◽

Cover Crop ◽

Field Experiments ◽

Negative Relationship ◽

Crop Growth ◽

Above Ground Biomass ◽

Crop Species ◽

Metarhizium Robertsii ◽

Cereal Rye ◽

Ground Biomass

Fungi in the genus Metarhizium (Hypocreales: Clavicipitaceae) are insect pathogens that can establish as endophytes and can benefit their host plant. In field experiments, we observed a positive correlation between the prevalence of M. robertsii and legume cover crops, and a negative relationship with brassicaceous cover crops and with increasing proportion of cereal rye in mixtures. Here, we report the effects of endophytic M. robertsii on three cover crop species under greenhouse conditions. We inoculated seeds of Austrian winter pea (Pisum sativum L., AWP), cereal rye (Secale cereale L.), and winter canola (Brassica napus L.) with conidia of M. robertsii to assess the effects of endophytic colonization on cover crop growth. We recovered M. robertsii from 59%, 46%, and 39% of seed-inoculated AWP, cereal rye, and canola plants, respectively. Endophytic M. robertsii significantly increased height and above-ground biomass of AWP and cereal rye but did not affect chlorophyll content of any of the cover crop species. Among inoculated plants from which we recovered M. robertsii, above-ground biomass of AWP was positively correlated with the proportion of colonized root but not leaf tissue sections. Our results suggest that winter cover crops may help to conserve Metarhizium spp. in annual cropping systems.

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Cover crop species affect mycorrhizae-mediated nutrient uptake and pest resistance in maize

Renewable Agriculture and Food Systems ◽

10.1017/s1742170519000061 ◽

2019 ◽

Vol 35 (5) ◽

pp. 467-474 ◽

Cited By ~ 8

Author(s):

Ebony G. Murrell ◽

Swayamjit Ray ◽

Mary E. Lemmon ◽

Dawn S. Luthe ◽

Jason P. Kaye

Keyword(s):

Nutrient Uptake ◽

Cover Crops ◽

Cover Crop ◽

European Corn Borer ◽

Pest Resistance ◽

Plant Nutrient ◽

Crop Species ◽

Maize Crop ◽

Corn Borer ◽

Maize Growth

AbstractArbuscular mycorrhizal fungi (AMF) can increase plant nutrient uptake and chemical defense production, both of which can improve plants’ ability to resist insect herbivory. Cover crops—non-commercial species planted in between cash crops in a crop rotation—can naturally alter both soil nutrients and AMF. We tested whether different cover crop species alter AMF colonization, plant nutrient status and plant–insect interactions in a subsequent maize crop. Cover crop species were either non-mycorrhizal, non-leguminous (canola, forage radish), mycorrhizal non-leguminous (cereal rye, oats), mycorrhizal leguminous (clover, pea) or absent (fallow). We measured the cascading consequences of cover crop treatment on maize root AMF colonization, maize growth and performance of an herbivorous insect (European corn borer) feeding on the maize. Maize AMF colonization was greater in plots previously planted with mycorrhizal (rye, oats) than non-mycorrhizal (canola, radish) cover crops or no cover crop (fallow). AMF colonization was linked to increased plant phosphorous and nitrogen, and maize growth increased with low plant N:P. Induced jasmonic acid pathway plant defenses increased with increasing maize growth and AMF colonization. European corn borer survivorship decreased with lower plant N:P, and insect development rate decreased with increased induced plant defenses. Our data describe a cascade in which cover crop species selection can increase or decrease mycorrhizal colonization of subsequent maize crop roots, which in turn impacts phosphorus uptake and may affect herbivory resistance in the maize. These results suggest that farmers could select cover crop species to manage nutrient uptake and pest resistance, in order to amend or limit fertilizer and pesticide use.

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The Correct Cover Crop Species Integrated with Slurry Can Increase Biomass, Quality and Nitrogen Cycling to Positively Affect Yields in a Subsequent Spring Barley Rotation

Agronomy ◽

10.3390/agronomy10111760 ◽

2020 ◽

Vol 10 (11) ◽

pp. 1760

Author(s):

Paul Cottney ◽

Lisa Black ◽

Ethel White ◽

Paul N. Williams

Keyword(s):

Cover Crops ◽

Cover Crop ◽

Crop Yields ◽

Spring Barley ◽

C:N Ratio ◽

Brassica Species ◽

Carbon Accumulation ◽

Soil Mineral Nitrogen ◽

Crop Species ◽

N Accumulation

The aim of this study is to identify species of cover crops that cause an increase in biomass and total nutrient accumulation in response to manure/slurry. This could improve nutrient efficiency and intensify the benefits from over-winter cover crops in arable rotations and improve following commercial crop yields. In a pot experiment, sixteen cover crops were grown for 100 days in response to slurry. Growth and nutrient (N, P, K, Mg and S) accumulation were measured, and then residue was reincorporated into the soil with spring barley (Hodeum vulgare L.) sown and harvested for yield. In response to slurry, tillage radish (Raphanus sativus L.) increased N accumulation by 101% due to a significant increase in biomass and % N (p < 0.05) over its relative control plots. Significant interactions between species and the application of slurry were found in cover crop biomass, cover crop and spring barley nutrient uptake, as well as cover crop carbon accumulation, particularly in the brassica species used. Slurry integrated with cover crops both reduced the cover crop C:N ratio and enhanced nutrient cycling compared to the control when soil mineral nitrogen (SMN) and spring barley crop N offtake were pooled. However, this was not observed in the legumes. This study shows that slurry integration with cover crops is a promising sustainable farming practice to sequester N and other macro-nutrients whilst providing a range of synergistic benefits to spring barley production when compared to unplanted/fallow land rotations. However, this advantage is subject to use of responsive cover crop species identified in this study.

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Carryover of Common Corn and Soybean Herbicides to Various Cover Crop Species

Weed Technology ◽

10.1614/wt-d-16-00062.1 ◽

2017 ◽

Vol 31 (1) ◽

pp. 21-31 ◽

Cited By ~ 19

Author(s):

Cody D. Cornelius ◽

Kevin W. Bradley

Keyword(s):

Cover Crops ◽

Cover Crop ◽

Production Systems ◽

Italian Ryegrass ◽

Hairy Vetch ◽

Crop Species ◽

Cereal Rye ◽

Crimson Clover ◽

Crop Establishment ◽

Biomass Reduction

The recent interest in cover crops as component of Midwest corn and soybean production systems has led to the need for additional research, including the effects of residual corn and soybean herbicide treatments on fall cover crop establishment. Field studies were conducted in 2013, 2014, and 2015 in Columbia, Missouri to investigate the effects of common residual herbicides applied in corn and soybean on establishment of winter wheat, tillage radish, cereal rye, crimson clover, winter oat, Austrian winter pea, Italian ryegrass, and hairy vetch. Cover crops were evaluated for stand and biomass reduction 28 d after emergence (DAE). Rainfall from herbicide application to cover crop seeding date was much greater in 2014 and 2015, which resulted in less carryover in these years compared to 2013. When averaged across all herbicides evaluated in these experiments, the general order of sensitivity of cover crops to herbicide carryover, from greatest to least was Austrian winter pea=crimson clover>oilseed radish>Italian ryegrass>hairy vetch>wheat >winter oat>cereal rye. Cereal rye had the fewest instances of biomass or stand reduction with only four out of the 27 herbicides adversely effecting establishment. Pyroxasulfone consistently reduced Italian ryegrass and winter oat biomass at least 67% in both the corn and soybean experiments. In the soybean experiment, imazethapyr- and fomesafen-containing products resulted in severe stand and biomass reduction in both years while flumetsulam-containing products resulted in the greatest carryover symptoms in the corn experiment. Results from these experiments suggest that several commonly used corn and soybean herbicides have the potential to hinder cover crop establishment, but the severity of damage will depend on weather, cover crop species, and the specific herbicide combination.

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