scholarly journals Can phosphorus application and cover cropping alter arbuscular mycorrhizal fungal communities and soybean performance after a five-year phosphorus-unfertilized crop rotational system?

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4606 ◽  
Author(s):  
Masao Higo ◽  
Ryohei Sato ◽  
Ayu Serizawa ◽  
Yuichi Takahashi ◽  
Kento Gunji ◽  
...  
Keyword(s):  
Cover Crop ◽  
Arbuscular Mycorrhizal ◽  
P Uptake ◽  
Flowering Stage ◽  
P Nutrition ◽  
Cover Cropping ◽  
Amf Diversity ◽  
P Application ◽  
Soybean Roots ◽  
Amf Communities

BackgroundUnderstanding diversity of arbuscular mycorrhizal fungi (AMF) is important for optimizing their role for phosphorus (P) nutrition of soybeans (Glycine max(L.) Merr.) in P-limited soils. However, it is not clear how soybean growth and P nutrition is related to AMF colonization and diversity of AMF communities in a continuous P-unfertilized cover cropping system. Thus, we investigated the impact of P-application and cover cropping on the interaction among AMF colonization, AMF diversity in soybean roots, soybean growth and P nutrition under a five-year P-unfertilized crop rotation.MethodsIn this study, we established three cover crop systems (wheat, red clover and oilseed rape) or bare fallow in rotation with soybean. The P-application rates before the seeding of soybeans were 52.5 and 157.5 kg ha−1in 2014 and 2015, respectively. We measured AMF colonization in soybean roots, soybean growth parameters such as aboveground plant biomass, P uptake at the flowering stage and grain yields at the maturity stage in both years. AMF community structure in soybean roots was characterized by specific amplification of small subunit rDNA.ResultsThe increase in the root colonization at the flowering stage was small as a result of P-application. Cover cropping did not affect the aboveground biomass and P uptake of soybean in both years, but the P-application had positive effects on the soybean performance such as plant P uptake, biomass and grain yield in 2015. AMF communities colonizing soybean roots were also significantly influenced by P-application throughout the two years. Moreover, the diversity of AMF communities in roots was significantly influenced by P-application and cover cropping in both years, and was positively correlated with the soybean biomass, P uptake and grain yield throughout the two years.DiscussionOur results indicated that P-application rather than cover cropping may be a key factor for improving soybean growth performance with respect to AMF diversity in P-limited cover cropping systems. Additionally, AMF diversity in roots can potentially contribute to soybean P nutrition even in the P-fertilized cover crop rotational system. Therefore, further investigation into the interaction of AMF diversity, P-application and cover cropping is required for the development of more effective P management practices on soybean growth performance.

scholarly journals Cover cropping can be a stronger determinant than host crop identity for arbuscular mycorrhizal fungal communities colonizing maize and soybean

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6403 ◽  
Author(s):  
Masao Higo ◽  
Yuya Tatewaki ◽  
Kento Gunji ◽  
Akari Kaseda ◽  
Katsunori Isobe
Keyword(s):  
Community Structure ◽  
Cover Crops ◽  
Cover Crop ◽  
Arbuscular Mycorrhizal ◽  
Crop Species ◽  
Cover Cropping ◽  
Cash Crops ◽  
Subsequent Crop ◽  
Soybean Roots ◽  
Amf Communities

BackgroundUnderstanding the role of communities of arbuscular mycorrhizal fungi (AMF) in agricultural systems is imperative for enhancing crop production. The key variables influencing change in AMF communities are the type of cover crop species or the type of subsequent host crop species. However, how maize and soybean performance is related to the diversity of AMF communities in cover cropping systems remains unclear. We therefore investigated which cover cropping or host identity is the most important factor in shaping AMF community structure in subsequent crop roots using an Illumina Miseq platform amplicon sequencing.MethodsIn this study, we established three cover crop systems (Italian ryegrass, hairy vetch, and brown mustard) or bare fallow prior to planting maize and soybean as cash crops. After cover cropping, we divided the cover crop experimental plots into two subsequent crop plots (maize and soybean) to understand which cover cropping or host crop identity is an important factor for determining the AMF communities and diversity both in maize and soybeans.ResultsWe found that most of the operational taxonomic units (OTUs) in root samples were common in both maize and soybean, and the proportion of common generalists in this experiment for maize and soybean roots was 79.5% according to the multinomial species classification method (CLAM test). The proportion of OTUs specifically detected in only maize and soybean was 9.6% and 10.8%, respectively. Additionally, the cover cropping noticeably altered the AMF community structure in the maize and soybean roots. However, the differentiation of AMF communities between maize and soybean was not significantly different.DiscussionOur results suggest cover cropping prior to planting maize and soybean may be a strong factor for shaping AMF community structure in subsequent maize and soybean roots rather than two host crop identities. Additionally, we could not determine the suitable rotational combination for cover crops and subsequent maize and soybean crops to improve the diversity of the AMF communities in their roots. However, our findings may have implications for understanding suitable rotational combinations between cover crops and subsequent cash crops and further research should investigate in-depth the benefit of AMF on cash crop performances in cover crop rotational systems.

Influence of arbuscular mycorrhizae on soil P dynamics, corn P-nutrition and growth in a ridge-tilled commercial field

2008 ◽  
Vol 88 (3) ◽  
pp. 283-294 ◽  
Author(s):  
Christine P Landry ◽  
Chantal Hamel ◽  
Anne Vanasse
Keyword(s):  
Arbuscular Mycorrhizae ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Dry Mass ◽  
Soil Disturbance ◽  
P Uptake ◽  
P Nutrition ◽  
Soil P ◽  
High Yields ◽  
Ridge Tillage

Ridge-tilled corn (Zea mays L.) could benefit from arbuscular mycorrhizal (AM) fungi. Under low soil disturbance, AM hyphal networks are preserved and can contribute to corn nutrition. A 2-yr study was conducted in the St. Lawrence Lowlands (Quebec, Canada) to test the effects of indigenous AM fungi on corn P nutrition, growth, and soil P in field cropped for 8 yr under ridge-tillage. Phosphorus treatments (0, 17, 35 kg P ha-1) were applied to AM-inhibited (AMI) (fungicide treated) and AM non-inhibited (AMNI) plots. Plant tissue and soil were sampled 22, 48 and 72 days after seeding (DAS). P dynamics was monitored in situ with anionic exchange membranes (PAEM) from seeding to the end of July. AMNI plants showed extensive AM colonization at all P rates. At 22 DAS, AMI plants had decreased growth in the absence of P inputs, while AMNI plants had higher dry mass (DM) and P uptake in unfertilized plots. The PAEM was lower in the AMNI unfertilized soils in 1998 and at all P rates in 1999, indicating an inverse relationship between P uptake and PAEM. At harvest, grain P content of AMNI plants was greater than that of AMI plants. In 1998, only AMI plants had decreased yield in the absence of P fertilization. In 1999, AMNI plants produced greater grain yield than AMI plants at all P rates. AM fungi improve the exploitation of soil P by corn thereby maintaining high yields while reducing crop reliance on P inputs in RT. Key words: Arbuscular mycorrhizae, ridge-tillage, soil P dynamics, corn, P nutrition

Vesicular-Arbuscular Mycorrhiza and Phosphorus Uptake of Chickpea Grown in Northern Syria

1992 ◽  
Vol 28 (4) ◽  
pp. 433-442 ◽  
Author(s):  
Edwin Weber ◽  
Eckhard George ◽  
Douglas P. Beck ◽  
Mohan C. Saxena ◽  
Horst Marschner
Keyword(s):  
Mycorrhizal Fungi ◽  
Field Experiments ◽  
Phosphorus Uptake ◽  
Arbuscular Mycorrhizal ◽  
P Uptake ◽  
Mycorrhizal Infection ◽  
P Fertilization ◽  
Flowering Stage ◽  
P Concentration ◽  
Vesicular Arbuscular

SUMMARYInoculation with vesicular-arbuscular mycorrhizal fungi (VAMF) improved growth of chick-pea (Cicer arielinum L.) and doubled phosphorus (P) uptake at low and intermediate levels of P fertilization in a pot experiment on sterilized low-P calcareous soil. In field experiments at Tel Hadya, northern Syria, growth, shoot P concentration and seed yield of spring-sown chickpea remained unaffected by inoculation with VAMF or by P fertilization. The mycorrhizal infection of chickpea was high (approximately 75% of root length mycorrhizal at the flowering stage) irrespective of inoculation with VAMF or P fertilization and may ensure efficient P uptake under field conditions.

scholarly journals Species diversity and drivers of arbuscular mycorrhizal fungal communities in a semi-arid mountain in China

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e4155 ◽  
Author(s):  
He Zhao ◽  
Xuanzhen Li ◽  
Zhiming Zhang ◽  
Yong Zhao ◽  
Jiantao Yang ◽  
...  
Keyword(s):  
Species Diversity ◽  
Soil Texture ◽  
Community Assembly ◽  
Arable Land ◽  
Arbuscular Mycorrhizal ◽  
Forest Land ◽  
Habitat Types ◽  
Amf Diversity ◽  
Amf Communities ◽  
Semi Arid

Arbuscular mycorrhizal fungi (AMF) play an essential role in complex ecosystems. However, the species diversity and composition of AMF communities remain unclear in semi-arid mountains. Further, it is not well understood if the characteristics of AMF community assemblies differ for different habitat types, e.g., agricultural arable land, artificial forest land, natural grassland, and bush/wood land. Here, using the high-throughput technology by Illumina sequencing on the MiSeq platform, we explored the species diversity and composition of soil AMF communities among different habitat types in a semi-arid mountain (Taihang Mountain, Mid-western region of China). Then, we analyzed the effect of nutrient composition and soil texture on AMF community assembly. Our results showed that members of the Glomus genera were predominated in all soil types. The distance-based redundancy analysis indicated that the content of water, available phosphorus, and available potassium were the most crucial geochemical factors that significantly affected AMF communities (p < 0.05). The analysis of the soil texture confirmed that AMF diversity was negatively correlated with soil clay content. The comparison of AMF diversity among the various habitat types revealed that the artificial forest land had the lowest AMF diversity in comparison with other land types. Our findings suggest that there were differences in species diversity and composition of soil AMF communities among different habitat types. These findings shed new light on the characteristics of community structure and drivers of community assembly in AMF in semi-arid mountains, and point to the potential importance of different habitat types on AMF communities.

scholarly journals Rapid response of arbuscular mycorrhizal fungal communities to short-term fertilization in an alpine grassland on the Qinghai-Tibet Plateau

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2226 ◽  
Author(s):  
Xingjia Xiang ◽  
Sean M. Gibbons ◽  
Jin-Sheng He ◽  
Chao Wang ◽  
Dan He ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal ◽  
Illumina Miseq ◽  
Tibet Plateau ◽  
Available Phosphorus ◽  
Short Term ◽  
Amf Diversity ◽  
Amf Communities ◽  
Arbuscular Mycorrhizal Fungal ◽  
The Impact ◽  
Qinghai Tibet Plateau

Background:The Qinghai-Tibet Plateau (QTP) is home to the vast grassland in China. The QTP grassland ecosystem has been seriously degraded by human land use practices and climate change. Fertilization is used in this region to increase vegetation yields for grazers. The impact of long-term fertilization on plant and microbial communities has been studied extensively. However, the influence of short-term fertilization on arbuscular mycorrhizal fungal (AMF) communities in the QTP is largely unknown, despite their important functional role in grassland ecosystems.Methods:We investigated AMF community responses to three years of N and/or P addition at an experimental field site on the QTP, using the Illumina MiSeq platform (PE 300).Results:Fertilization resulted in a dramatic shift in AMF community composition and NP addition significantly increased AMF species richness and phylogenetic diversity. Aboveground biomass, available phosphorus, and NO3−were significantly correlated with changes in AMF community structure. Changes in these factors were driven by fertilization treatments. Thus, fertilization had a large impact on AMF communities, mediated by changes in aboveground productivity and soil chemistry.Discussion:Prior work has shown how plants often lower their reliance on AMF symbioses following fertilization, leading to decrease AMF abundance and diversity. However, our study reports a rise in AMF diversity with fertilization treatment. Because AMF can provide stress tolerance to their hosts, we suggest that extreme weather on the QTP may help drive a positive relationship between fertilizer amendment and AMF diversity.

Optimised nitrogen fertiliser management achieved higher diversity of arbuscular mycorrhiza fungi and high-yielding maize (Zea mays L.)

2015 ◽  
Vol 66 (7) ◽  
pp. 706 ◽  
Author(s):  
Xiaojing Wang ◽  
Xinxin Wang ◽  
Gu Feng
Keyword(s):  
Zea Mays ◽  
Mycorrhizal Fungi ◽  
Zea Mays L ◽  
Crop Yields ◽  
Arbuscular Mycorrhizal ◽  
Agricultural Landscapes ◽  
High Rate ◽  
Amf Diversity ◽  
Intensively Managed ◽  
Amf Communities

The integrated soil–crop system management (ISSM) approach can potentially mitigate the loss of biodiversity in agricultural landscapes, ensuring crop yield with lower nitrogen (N) fertiliser input and minimised environmental pollution. The aim of this study was to test the hypotheses that overuse of N fertiliser could reduce the biodiversity of arbuscular mycorrhizal fungi (AMF) and that ISSM could help to maintain higher AMF biodiversity than the conventionally managed system in maize (Zea mays L.). The AMF community composition under three different treatments (conventionally managed, N-optimised and non-N-fertilised fields) was assessed by using both spore-based morphological taxonomy and DNA-based T-RFLP fingerprinting approaches. Maize roots in intensively managed fields formed functioning mycorrhizal symbioses even when a high rate of N fertiliser was applied. AMF diversity was higher under optimised N input, whereas AMF richness decreased when more N fertiliser was used. The N-optimised farms had AMF communities similar to those in the conventionally managed fields. The ISSM approach is recommended for sustaining crop yields without incurring continuing environmental costs and for maintaining AMF communities in intensively managed agro-ecosystems, especially in rapidly developing countries.

scholarly journals Inoculation with highly-related mycorrhizal fungal siblings, and their interaction with plant genoptypes, strongly shapes tropical mycorrhizal fungal community structure

2020 ◽  
Author(s):  
Yuli Marcela Ordoñez ◽  
Lucas Villard ◽  
Isabel Ceballos ◽  
Frédéric G Masclaux ◽  
Alia Rodriguez ◽  
...  
Keyword(s):  
Community Structure ◽  
Mycorrhizal Fungi ◽  
Crop Yields ◽  
Arbuscular Mycorrhizal ◽  
Tropical Soils ◽  
Mycorrhizal Symbiosis ◽  
Fungal Community Structure ◽  
Amf Diversity ◽  
Amf Communities ◽  
The Impact

Arbuscular mycorrhizal fungi (AMF) have the potential to increase crop yields and all globally important crops form the mycorrhizal symbiosis. Only a few studies have investigated the impact of introduced AMF on local AMF communities and most studies have only investigated effects of one isolate. We studied the impact on AMF community structure of inoculating roots of the globally important crop cassava with highly genetically-related clonal siblings of two genetically different Rhizophagus irregularis isolates. We hypothesized that inoculation with R. irregularis siblings differentially influences the structure and the diversity of the pre-existing AMF community colonizing cassava. Alpha and beta taxonomic and phylogenetic AMF diversity were strongly and significantly altered differentially following inoculation with sibling AMF progeny. In most cases, the effects were also cassava-genotype specific. Although biomass production and AMF colonization were also both differentially affected by inoculation with sibling R. irregularis progeny these variables were not correlated with changes in the AMF community structure. The results highlight that investigations on the impact of an introduced AMF species, that use only one isolate, are unlikely to be representative of the overall effects of that AMF species and that the genetic identity of the host must be considered. The amount of inoculum added was very small and effects were observed 12 months following inoculation. That such a small amount of almost genetically identical fungal inoculum can strongly differentially influence AMF community structure 12 months following inoculation, indicates that AMF communities in tropical soils are not very resistant to perturbation.

scholarly journals Arbuscular Mycorrhizal Fungal Communities in Organic and Conventional Onion Crops in the Columbia Basin of the Pacific Northwest United States

2018 ◽  
Vol 2 (4) ◽  
pp. 194-207 ◽  
Author(s):  
Anne Jean’ne Knerr ◽  
David Wheeler ◽  
Dan Schlatter ◽  
Dipak Sharma-Poudyal ◽  
Lindsey J. du Toit ◽  
...  
Keyword(s):  
Pacific Northwest ◽  
Mycorrhizal Fungi ◽  
Root Colonization ◽  
Arbuscular Mycorrhizal ◽  
Columbia Basin ◽  
Metam Sodium ◽  
Operational Taxonomic Units ◽  
Amf Diversity ◽  
The Pacific ◽  
Amf Communities

Onions are highly responsive to arbuscular mycorrhizal fungi (AMF), but little is known about AMF communities in onion crops (∼10,000 ha) in the semiarid, irrigated region of the Columbia Basin of Washington and Oregon. AMF communities and root colonization were compared in organic and conventional onion fields, and between paired conventional fields that were fumigated or not with metam sodium. AMF were detected in all fields at all sampling times, with no differences in root colonization of onions used to bait soil from organic versus conventional and fumigated versus nonfumigated fields. However, AMF colonization of roots of onion plants sampled midsummer was greater in organic versus conventional fields (67 versus 51%) and less in fumigated versus nonfumigated conventional fields (45 versus 67%). Pyrosequencing identified four AMF genera (Glomus, Claroideoglomus, Paraglomus, and Diversispora) and four dominant operational taxonomic units (OTUs) (Glomus mosseae (Funneliformis mosseae), Glomus Whitfield type 17, Claroideoglomus lamellosum, and Glomus MO_G17). AMF community structure in roots of onion plants collected from crops midsummer was different in organic versus conventional crops, with greater AMF diversity and richness in organic than conventional crops. There was no effect of organic versus conventional crops on dominant OTUs, but several low-abundance OTUs in organic fields were not detected in conventional fields. There was no consistent effect of metam sodium chemigation on AMF communities in onion crops. Overall, cropping practices in organic versus conventional onion production, and the use of metam sodium soil fumigation by center-pivot chemigation do not appear to be major drivers of AMF communities.

scholarly journals Succession of endophytic fungi and arbuscular mycorrhizal fungi associated with the growth of plant and their correlation with secondary metabolites in the roots of plants

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hanli Dang ◽  
Tao Zhang ◽  
Zhongke Wang ◽  
Guifang Li ◽  
Wenqin Zhao ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Arbuscular Mycorrhizal Fungi ◽  
Endophytic Fungi ◽  
Relative Abundance ◽  
Mycorrhizal Fungi ◽  
Growth Period ◽  
Arbuscular Mycorrhizal ◽  
Amf Diversity ◽  
Metabolite Accumulation ◽  
Amf Communities

Abstract Background To decipher the root and microbial interaction, secondary metabolite accumulation in roots and the microbial community’s succession model during the plant’s growth period demands an in-depth investigation. However, till now, no comprehensive study is available on the succession of endophytic fungi and arbuscular mycorrhizal fungi (AMF) with roots of medicinal licorice plants and the effects of endophytic fungi and AMF on the secondary metabolite accumulation in licorice plant’s root. Results In the current study, interaction between root and microbes in 1–3 years old medicinal licorice plant’s root and rhizospheric soil was investigated. Secondary metabolites content in licorice root was determined using high-performance liquid chromatography (HPLC). The composition and diversity of endophytic and AMF in the root and soil were deciphered using high-throughput sequencing technology. During the plant’s growth period, as compared to AMF, time and species significantly affected the diversity and richness of endophytic fungi, such as Ascomycota, Basidiomycota, Fusarium, Cladosporium, Sarocladium. The growth period also influenced the AMF diversity, evident by the significant increase in the relative abundance of Glomus and the significant decrease in the relative abundance of Diversispora. It indicated a different succession pattern between the endophytic fungal and AMF communities. Meanwhile, distance-based redundancy analysis and Mantel tests revealed root’s water content and secondary metabolites (glycyrrhizic acid, liquiritin, and total flavonoids), which conferred endophytic fungi and AMF diversity. Additionally, plant growth significantly altered soil’s physicochemical properties, which influenced the distribution of endophytic fungal and AMF communities. Conclusions This study indicated a different succession pattern between the endophytic fungal and AMF communities. During the plant’s growth period, the contents of three secondary metabolites in roots increased per year, which contributed to the overall differences in composition and distribution of endophytic fungal and AMF communities. The endophytic fungal communities were more sensitive to secondary metabolites than AMF communities. The current study provides novel insights into the interaction between rhizospheric microbes and root exudates.

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