scholarly journals Selection of Beauveria bassiana (Hypocreales: Cordycipitaceae) strains to control Xyleborus affinis (Curculionidae: Scolytinae) females

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9472 ◽  
Author(s):  
Jesús E. Castrejón-Antonio ◽  
Patricia Tamez-Guerra ◽  
Roberto Montesinos-Matías ◽  
Maria J. Ek-Ramos ◽  
Paul M. Garza-López ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Principal Component ◽  
Preliminary Test ◽  
Persea Americana ◽  
Ambrosia Beetle ◽  
Tube Length ◽  
Germ Tube Length ◽  
Two Stages ◽  
Mexican Strains ◽  
Selection Of

Background Xyleborus affinis Eichhoff (Coleoptera: Curculionidae) is an ambrosia beetle reported to affect avocado trees (Persea americana Mill.). The use of the entomopathogenic fungus (EPF) Beauveria bassiana (Bals.-Criv.) Vuill. for ambrosia beetle control represents an alternative to insecticides. Methods This study was designed in two stages to select B. bassiana strains with potential to control X. affinis females. In the first stage, 19 B. bassiana Mexican strains from EPF collection, isolated from Coleoptera (CHE-CNRCB, http://www.gob.mx/senasica/documentos/coleccion-de-hongos-entomopatogenos), were tested. Analyses included radial growth rate, conidial yield, spore germination, and germ tube length. Results were analysed by Principal Component Analysis (PCA) to identify clusters within favourable growth phenotypes. For the second stage, 10 selected strains were re-analysed for virulence-related metabolic characteristic, including cell wall-bound cuticle-degrading enzymes–Pr1-like proteases and β-N-acetyl glucosaminidases (NAGase) chitinases, conidial hydrophobicity and monopolar germination parameters. A second PCA analysis was run for those virulence parameters analysed, and upon results strains CHE-CNRCB 44, 171, 431 and 485 were selected and tested against X. affinis females. Females were treated with a 1 × 108 conidia mL−1 suspension (recommended rate), using a Potter Tower. Results All strains showed insecticidal activity, inducing up to 58% mortality; about 30% dead beetles developed aerial mycelia (CHE-CNRCB 485) and the fastest mortality rate was t0 = 1.95 (CHE-CNRCB 44). Conclusion Since all selected strains showed virulence against X. affinis females, results indicated the possibility of selecting B. bassiana strains based on multiple metabolic attributes, as a preliminary test to perform bioassays against order-related target insects.

scholarly journals Modeling, comparison and evaluation of one-DOF six-bar bioinspired jumping leg mechanisms

2021 ◽  
Vol 13 (2) ◽  
pp. 168781402199295
Author(s):  
Ziqiang Zhang ◽  
Qi Yang ◽  
Xingkun Liu ◽  
Chuanzhong Zhang ◽  
Jinnong Liao
Keyword(s):  
Center Of Mass ◽  
Principal Component ◽  
Analysis Method ◽  
Index Distribution ◽  
Inertial Moment ◽  
Structure Index ◽  
Mean And Variance ◽  
Research Objects ◽  
Leg Mechanisms ◽  
Selection Of

One degree-of-freedom (DOF) jumping leg has the advantages of simple control and high stiffness, and it has been widely used in bioinspired jumping robots. Compared with four-bar jumping leg, six-bar jumping leg mechanism can make the robot achieve more abundant motion rules. However, the differences among different configurations have not been analyzed, and the choice of configurations lacks basis. In this study, five Watt-type six-bar jumping leg mechanisms were selected as research objects according to the different selection of equivalent tibia, femur and trunk link, and a method for determining the dimension of the jumping leg was proposed based on the movement law of jumping leg of locust in take-off phase. On this basis, kinematics indices (sensitivity of take-off direction angle and trunk attitude angle), dynamics indices (velocity loss, acceleration fluctuation, and mean and variance of total inertial moment) and structure index (distribution of center of mass) were established, and the differences of different configurations were compared and analyzed in detail. Finally, according to the principal component analysis method, the optimal selection method for different configurations was proposed. This study provides a reference for the design of one DOF bioinspired mechanism.

Selection of entomopathogenic fungus Beauveria bassiana (Deuteromycotina: Hyphomycetes) for the biocontrol of Dendroctonus ponderosae (Coleoptera: Curculionidae, Scolytinae) in Western Canada

2021 ◽  
Vol 105 (6) ◽  
pp. 2541-2557
Author(s):  
Albert Remus R. Rosana ◽  
Stanley Pokorny ◽  
Jennifer G. Klutsch ◽  
Cherry Ibarra-Romero ◽  
Randy Sanichar ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungus ◽  
Dendroctonus Ponderosae ◽  
Western Canada ◽  
Fungus Beauveria Bassiana ◽  
Selection Of

Morph-specific correlations between floral traits in a distylous Ophiorrhiza napoensis (Rubiaceae) population in southern China

2001 ◽  
Vol 17 (5) ◽  
pp. 719-728 ◽  
Author(s):  
HIROSHI KUDOH ◽  
TAKASHI SUGAWARA ◽  
SUGONG WU ◽  
JIN MURATA
Keyword(s):  
Southern China ◽  
Principal Component ◽  
Corolla Tube ◽  
Floral Traits ◽  
Evergreen Forest ◽  
Tube Length ◽  
Multiple Traits ◽  
Floral Trait ◽  
Subtropical Evergreen Forest ◽  
Positive Correlations

Floral trait correlations were compared between the two flower morphs of a distylous Ophiorrhiza napoensis population in a subtropical evergreen forest at the Defu Natural Animal Preserve, Guangxi, China. Common principal component analyses indicated that overall patterns in correlations among floral traits were morph specific in the study population. Strong positive correlations (r > 0.9) between anther height and corolla-tube length were found in both morphs. Stigma height correlated positively with corolla-tube length in the long-styled morph (r = 0.843), but not in the short-styled morph (r = −0.018). Flower-morph-specific correlation suggests that natural selection by pollinators has moulded trait covariance among floral traits. Because morph-specific correlations are expressed as the patterns of within-morph variation among multiple traits, putative genes responsible for the stigma-corolla tube correlation should not link to the supergene for sex-organ reciprocity between the morphs, but their expression is limited in the long-styled morph.

scholarly journals Categorization of the main descriptors of different ampicillin crystal habits

2010 ◽  
Vol 46 (4) ◽  
pp. 679-685 ◽  
Author(s):  
Oscar Flórez-Acosta ◽  
Gloria Tobón-Zapata ◽  
Jaime Valencia-Velasquez
Keyword(s):  
Principal Component Analysis ◽  
Raw Materials ◽  
Principal Component ◽  
Crystal Habit ◽  
Morphological Properties ◽  
Effective Diameter ◽  
Fourier Descriptors ◽  
Morphological Descriptors ◽  
Digital Methods ◽  
Selection Of

With the purpose of enabling the analysis by digital methods of particles of multisource pharmaceutical raw materials, this study analyzed different crystal habits of ampicillin particles, by grouping the external shapes obtained from 3 different solvents (acetonitrile, ethanol, and methanol), thereby reducing the number of descriptors necessary to adequately represent each shape. For this purpose, a selection of morphological descriptors was used including: circularity, roughness, roundness, compactness, aspect ratio, effective diameter, solidity, convexity, fractal dimension, and 10 Complex Fourier descriptors. These measures cover highly diverse morphological properties and define the crystal habit of a particle. Principal Component Analysis (PCA) and the Cluster Analysis (CA) were the grouping techniques used, which demonstrated the possibility of using between 2 and 4 descriptors instead of the 18 proposed initially.

scholarly journals Selection of cowpea progenies with enhanced drought-tolerance traits using principal component analysis

2015 ◽  
Vol 14 (4) ◽  
pp. 15981-15987 ◽  
Author(s):  
C.C. Sousa ◽  
K.J. Damasceno-Silva ◽  
E.A. Bastos ◽  
M.M. Rocha
Keyword(s):  
Principal Component Analysis ◽  
Drought Tolerance ◽  
Principal Component ◽  
Component Analysis ◽  
Selection Of

scholarly journals Glucosylceramide Plays a Role in Fungal Germination, Lipid Raft Organization and Biofilm Adhesion of the Pathogenic Fungus Scedosporium aurantiacum

2020 ◽  
Vol 6 (4) ◽  
pp. 345
Author(s):  
Victor Pereira Rochetti ◽  
Rodrigo Rollin-Pinheiro ◽  
Evely Bertulino de Oliveira ◽  
Mariana Ingrid Dutra da Silva Xisto ◽  
Eliana Barreto-Bergter
Keyword(s):  
Cell Surface ◽  
Pathogenic Fungus ◽  
Clinical Manifestations ◽  
Tube Length ◽  
Total Biomass ◽  
Fungal Cell ◽  
Germ Tube Length ◽  
Wide Range ◽  
Fluorescent Stain ◽  
Altered Lipid

Infections caused by Scedosporium species present a wide range of clinical manifestations, from superficial to disseminated, especially in immunocompromised patients. Glucosylceramides (GlcCer) are glycosphingolipids found on the fungal cell surface and play an important role in growth and pathogenicity processes in different fungi. The present study aimed to evaluate the structure of GlcCer and its role during growth in two S. aurantiacum isolates. Purified GlcCer from both isolates were obtained and its chemical structure identified by mass spectrometry. Using ELISA and immunofluorescence techniques it was observed that germination and NaOH-treatment of conidia favor GlcCer exposure. Monoclonal anti-GlcCer antibody reduced germination when cultivated with the inhibitor of melanin synthesis tricyclazole and also reduced germ tube length of conidia, both cultivated or not with tricyclazole. It was also demonstrated that anti-GlcCer altered lipid rafts organization, as shown by using the fluorescent stain filipin, but did not affect the susceptibility of the cell surface to damaging agents. Anti-GlcCer reduced total biomass and viability in biofilms formed on polystyrene plates. In the presence of anti-GlcCer, germinated S. aurantiacum conidia and biofilms could not adhere to polystyrene with the same efficacy as control cells. These results highlight the relevance of GlcCer in growth processes of S. aurantiacum.

scholarly journals Stability of Wheat Genotypes by AMMI and Yield Evaluated under Peninsular Zone of India

2021 ◽  
Author(s):  
Ajay Verma ◽  
G.P. Singh
Keyword(s):  
Weighted Average ◽  
Principal Component ◽  
Efficient Estimation ◽  
High Yield ◽  
First Year ◽  
Wheat Genotypes ◽  
Stability Measure ◽  
Linear Behaviour ◽  
The Stability ◽  
Selection Of

Background: Efficient estimation of main and interaction effects under multi environment trials had been carried out by AMMI. Studies with low proportion of the variance explained by first interaction principal component IPCA1 under AMMI analysis had been reported biased interpretation for the stability of the genotypes.Methods: Weighted Average of Absolute scores (WAASB), quantitative stability measure had been recommended for selection of productive genotypes with broad adaptation. The selection of promising genotypes had been assisted by use of superiority index (WAASBY) that considered simultaneous use of yield and stability by allowing variable weighting mechanism for yield and stability.Result: Wheat GW509 and HI1633 genotypes had been identified by stability measure WAASB for the first year. While Superiority index considered stability and high yield settled for HI1633 and Raj4083 genotypes. Analytic measures of adaptability PRVG and MHPRVG observed suitability of HI1633 and Raj 4083. SI expressed positive linear behaviour with yield, MHPRVG and PRVG measures. While stability measure WAASB maintained moderate negative correlation with yield, SI, MHPRVG and PRVG. Wheat genotypes HI1633, HI1641 and HI1646 identified by stability measure for the next year of study. Superiority index selected HI1641, HI1633 and MACS6752 genotypes. SI expressed direct linear relation with yield, MHPRVG and PRVG. Stability measure exhibited indirect relationships with SI, MHPRVG, PRVG and yield Wheat genotypes HI1633, Raj 4083 for first year and HI1641, MACS6752 for the second year of study.

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