scholarly journals Arabidopsis thaliana exudates induce growth and proteomic changes in Gluconacetobacter diazotrophicus

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9600
Author(s):  
Tamires Cruz dos Santos ◽  
Mariana Ramos Leandro ◽  
Clara Yohana Maia ◽  
Patrícia Rangel ◽  
Fabiano S. Soares ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Bacterial Growth ◽  
Proteomic Analysis ◽  
Reverse Genetics ◽  
Fatty Acid Biosynthesis ◽  
Gluconacetobacter Diazotrophicus ◽  
Comparative Proteomic Analysis ◽  
Plant Exudates ◽  
Insertion Mutants

Background Plants interact with a variety of microorganisms during their life cycle, among which beneficial bacteria deserve special attention. Gluconacetobacter diazotrophicus is a beneficial bacterium able to fix nitrogen and promote plant growth. Despite its biotechnological potential, the mechanisms regulating the interaction between G. diazotrophicus and host plants remain unclear. Methods We analyzed the response of G. diazotrophicus to cocultivation with Arabidopsis thaliana seedlings. Bacterial growth in response to cocultivation and plant exudates was analyzed. Through comparative proteomic analysis, G. diazotrophicus proteins regulated during cocultivation were investigated. Finally, the role of some up-accumulated proteins in the response G. diazotrophicus to cocultivation was analyzed by reverse genetics, using insertion mutants. Results Our results revealed the induction of bacterial growth in response to cocultivation. Comparative proteomic analysis identified 450 bacterial proteins, with 39 up-accumulated, and 12 down-accumulated in response to cocultivation. Among the up-accumulated pathways, the metabolism of pentoses and protein synthesis were highlighted. Proteins potentially relevant to bacterial growth response such as ABC-F-Etta, ClpX, Zwf, MetE, AcnA, IlvC, and AccC were also increased. Reverse genetics analysis, using insertion mutants, revealed that the lack of ABC-F-Etta and AccC proteins severely affects G. diazotrophicus response to cocultivation. Our data demonstrated that specific mechanisms are activated in the bacterial response to plant exudates, indicating the essential role of “ribosomal activity” and “fatty acid biosynthesis” in such a process. This is the first study to demonstrate the participation of EttA and AccC proteins in plant-bacteria interactions, and open new perspectives for understanding the initial steps of such associations.

Comparative proteomic analysis provides insight into the biological role of protein phosphatase inhibitor-2 from Arabidopsis

2017 ◽  
Vol 165 ◽  
pp. 51-60 ◽  
Author(s):  
Nagib Ahsan ◽  
Mingjie Chen ◽  
Fernanda Salvato ◽  
Rashaun S. Wilson ◽  
R. Shyama Prasad Rao ◽  
...  
Keyword(s):  
Protein Phosphatase ◽  
Proteomic Analysis ◽  
Biological Role ◽  
Comparative Proteomic Analysis ◽  
Phosphatase Inhibitor ◽  
Protein Phosphatase Inhibitor ◽  
Insight Into

scholarly journals Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in Arabidopsis thaliana (L.) with a reverse genetics approach

2019 ◽  
Vol 71 (3) ◽  
pp. 443-453
Author(s):  
Milan Dragicevic ◽  
Katarina Cukovic ◽  
Snezana Zdravkovic-Korac ◽  
Ana Simonovic ◽  
Milica Bogdanovic ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Glutamine Synthetase ◽  
Reverse Genetics ◽  
Technological Development ◽  
Expression Patterns ◽  
Dry Weight ◽  
Grain Filling ◽  
Kinetic Properties ◽  
Plant Nitrogen

Glutamine synthetase (E.C. 6.3.1.2) is a key enzyme of plant nitrogen metabolism that assimilates ammonia into glutamine. The Arabidopsis thaliana genome encodes one chloroplastic (GLN2) and five cytosolic (GLN1;1 ? GLN1;5) isoforms with different expression patterns, kinetic properties, regulation and functions. Physiological roles of different isoforms have been elucidated mainly by studying knockout mutants. However, the role of GLN1;5, which is expressed in dry seeds, remains unknown. To clarifty the function of GLN1;5, we studied a GLN1;5 knockout line (GLN1;5KO) homozygous for T-DNA insertion within the GLN1;5. GLN1;5 deficiency results in a phenotype with slightly delayed bolting and fewer siliques. The dry weight of GLN1;5KO seeds was 73.3% of wild-type (WT) seed weight, with seed length 90.9% of WT seeds. Finally, only 18.33% of the mutant seeds germinated in water within 10 days in comparison to 34.67% of WT seeds. KNO3 strongly stimulated germination of both GLN1;5KO and WT seeds, while germination in the presence of increasing NH4Cl concentrations potentiated the differences between the two genotypes. It can be concluded that GLN1;5 activity supports silique development and grain filling and that it has a role in ammonium reassimilation in the seed, as well as assimilation and/or detoxification of ammonium from the environment. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. ON173024 and Grant no. ON173015]

scholarly journals Comparative Proteomic Analysis of tPVAT during Ang II Infusion

Biomedicines ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 1820
Author(s):  
Xiuying Liang ◽  
Haijing Guan ◽  
Jingwen Sun ◽  
Yan Qi ◽  
Wenjuan Yao
Keyword(s):  
Proteomic Analysis ◽  
Vascular Function ◽  
Fatty Acid Biosynthesis ◽  
Uncoupling Protein ◽  
Ang Ii ◽  
Mice Model ◽  
Bioinformatics Analyses ◽  
Perivascular Adipose Tissue ◽  
Comparative Proteomic Analysis

Perivascular adipose tissue (PVAT) homeostasis plays an important role in maintaining vascular function, and PVAT dysfunction may induce several pathophysiological situations. In this study, we investigated the effect and mechanism of the local angiotensin II (Ang II) on PVAT. High-throughput comparative proteomic analysis, based on TMT labeling combined with LC-MS/MS, were performed on an in vivo Ang II infusion mice model to obtain a comprehensive view of the protein ensembles associated with thoracic PVAT (tPVAT) dysfunction induced by Ang II. In total, 5037 proteins were confidently identified, of which 4984 proteins were quantified. Compared with the saline group, 145 proteins were upregulated and 146 proteins were downregulated during Ang II-induced tPVAT pathogenesis. Bioinformatics analyses revealed that the most enriched GO terms were annotated as gene silencing, monosaccharide binding, and extracellular matrix. In addition, some novel proteins, potentially associated with Ang II infusion, were identified, such as acyl-CoA carboxylase α, very long-chain acyl-CoA synthetase (ACSVL), uncoupling protein 1 (UCP1), perilipin, RAS protein-specific guanine nucleotide-releasing factor 2 (RasGRF2), and hypoxia inducible factor 1α (HIF-1α). Ang II could directly participate in the regulation of lipid metabolism, transportation, and adipocyte differentiation by affecting UCP1 and perilipin. Importantly, the key KEGG pathways were involved in fatty acid biosynthesis, FABP3-PPARα/γ, RasGRF2-ERK-HIF-1α, RasGRF2-PKC-HIF-1α, and STAT3-HIF-1α axis. The present study provided the most comprehensive proteome profile of mice tPVAT and some novel insights into Ang II-mediated tPVAT dysfunction and will be helpful for understanding the possible relationship between local RAS activation and PVAT dysfunction.

scholarly journals Ectopic Expression of Fiber Related Gbwri1 Complements Seed Phenotype in Arabidopsis thaliana

2021 ◽  
Vol 25 (01) ◽  
pp. 123-130
Author(s):  
Mahnoor Imran
Keyword(s):  
Arabidopsis Thaliana ◽  
Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
Ectopic Expression ◽  
Acid Biosynthesis ◽  
Functional Homology ◽  
Normal Seedling ◽  
Transgenic Lines ◽  
Protein Isoform

WRINKLED1 belongs to AP2/EREB family of transcription factors whose role has been well established in seed oil biosynthesis. The objective of the study was to trace the role of fiber related Gbwri1 in seed development and fatty acid biosynthesis. In this study, we isolated a transcript from elite fiber producing cotton (Gossypium barbadense), which is over-expressed in G. barbadense fibers as compared to G. hirsutum and G. arboreum. The putative protein encoded by this transcript exhibited homology in specific domains and protein structure with WRINKLED1 of Arabidopsis thaliana and was thus designated as Gbwri1. In this study, we investigated the functional homology of fiber elongation related Gbwri1 with fatty acid biosynthesis regulator Atwri1. Ectopic expression of Gbwri1 in wri1-3 mutant of A. thaliana was analyzed. In the transgenic lines of A. thaliana, Gbwri1 resumed the seed weight, seed area, and surface morphology to the wild type. Gbwri1 transformation rescued the wrinkled phenotype of wri1-3 mutants by resuming the expression of fatty acid biosynthesis genes biotin carboxyl carrier protein isoform 2 (bccp2) and keto-ACP synthase 1 (kas1). Moreover, the seedling development of transgenic lines on non-sucrose medium demonstrated that the Gbwri1 was able to regulate the supply of sucrose for normal seedling establishment. Our results showed that the transformation of Gbwri1 in A. thaliana wri1-3 mutant was able to complement wri1-3 impaired phenotype. Thus, Gbwri1 is involved in cotton fiber development and fatty acid biosynthesis in seeds. © 2021 Friends Science Publishers

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