scholarly journals Antioxidant and Protective Effects of Leonurus sibiricus L. Extract on Ultraviolet B (UVB)-induced Damage in Human Keratinocytes

2016 ◽  
Vol 29 (1) ◽  
pp. 11-19 ◽  
Author(s):  
Tae Yeon Kim ◽  
Seon A Jang ◽  
Yong Byung Chae ◽  
Jong Phil Bak
Keyword(s):  
Human Keratinocytes ◽  
Ultraviolet B ◽  
Protective Effects ◽  
Leonurus Sibiricus

scholarly journals UVB protective effects of Sargassum horneri through the regulation of Nrf2 mediated antioxidant mechanism

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Eui Jeong Han ◽  
Seo-Young Kim ◽  
Hee-Jin Han ◽  
Hyun-Soo Kim ◽  
Kil-Nam Kim ◽  
...  
Keyword(s):  
Skin Barrier ◽  
Human Keratinocytes ◽  
Underlying Mechanism ◽  
Ultraviolet B ◽  
Sargassum Horneri ◽  
Cellular Damage ◽  
Protective Effects ◽  
Skin Damage ◽  
Antioxidant Mechanism ◽  
Induced Apoptosis

AbstractThe present study aimed to evaluate the protective effect of a methanol extract of Sargassum horneri (SHM), which contains 6-hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one (HTT) and apo-9′-fucoxanthinone, against ultraviolet B (UVB)-induced cellular damage in human keratinocytes and its underlying mechanism. SHM significantly improved cell viability of UVB-exposed human keratinocytes by reducing the generation of intracellular reactive oxygen species (ROS). Moreover, SHM inhibited UVB exposure-induced apoptosis by reducing the formation of apoptotic bodies and the populations of the sub-G1 hypodiploid cells and the early apoptotic cells by modulating the expression of the anti- and pro-apoptotic molecules, Bcl-2 and Bax, respectively. Furthermore, SHM inhibited NF-κB p65 activation by inducing the activation of Nrf2/HO-1 signaling. The cytoprotective and antiapoptotic activities of SHM are abolished by the inhibition of HO-1 signaling. In further study, SHM restored the skin dryness and skin barrier disruption in UVB-exposed human keratinocytes. Based to these results, our study suggests that SHM protects the cells against UVB-induced cellular damages through the Nrf2/HO-1/NF-κB p65 signaling pathway and may be potentially useful for the prevention of UVB-induced skin damage.

scholarly journals Fucoidan Fractionated from Sargassum coreanum via Step-Gradient Ethanol Precipitation Indicate Promising UVB-Protective Effects in Human Keratinocytes

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 347
Author(s):  
Ilekuttige Priyan Shanura Fernando ◽  
Mawalle Kankanamge Hasitha Madhawa Dias ◽  
Dissanayaka Mudiyanselage Dinesh Madusanka ◽  
Eui Jeong Han ◽  
Min Ju Kim ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Human Keratinocytes ◽  
Industrial Applications ◽  
Ultraviolet B ◽  
Related Factor ◽  
Protective Effects ◽  
Ethanol Precipitation ◽  
Extraction And Purification ◽  
Antioxidant Defense Enzymes ◽  
Wide Range

Fucoidans exhibit a wide range of bioactivities and receive significant attention in functional food and cosmetic research. Industrial applications of fucoidan are limited partially due to high extraction and purification costs. The present study implements an enzyme-assisted extraction and step-gradient ethanol precipitation for fractionating fucoidan from Sargassum coreanum based on its charge and molecular weight and evaluation of ultraviolet B (UVB) protective effects in human keratinocytes (HaCaT). The fucoidan fraction SCOC4 indicated higher fucose and sulfate contents with Fourier-transform infrared and 1H NMR spectral patterns resembling fucoidans. SCOC4 dose-dependently abated UVB-induced keratinocyte damage via suppressing intracellular reactive oxygen species, apoptotic body formation, DNA damage via suppressing mitochondria-mediated apoptosis. UVB-protective effects of SCOC4 were further attributable to the augmentation of nuclear factor erythroid 2-related factor 2 mediated cellular antioxidant defense enzymes. Step-gradient ethanol precipitation was a convenient approach of fractionating fucoidans based on molecular weight and charge (depend on the degree of sulfation). Further evaluation of seasonal variations, biocompatibility parameters, efficacy, and shelf life may widen the use of S. coreanum fucoidans in developing UVB-protective cosmetics and functional foods.

Protective effects of myricetin against ultraviolet-B-induced damage in human keratinocytes

2010 ◽  
Vol 24 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Jheng-Hua Huang ◽  
Chieh-Chen Huang ◽  
Jia-You Fang ◽  
Cheng Yang ◽  
Chi-Ming Chan ◽  
...  
Keyword(s):  
Human Keratinocytes ◽  
Ultraviolet B ◽  
Protective Effects

A novel apoptosis inducer, apoptosis signal regulating kinase (ASK1), is involved in ultraviolet B-induced apoptosis of cultured human keratinocytes

1998 ◽  
Vol 16 ◽  
pp. S69 ◽  
Author(s):  
Koji Sayama ◽  
Hidenori Ichijo ◽  
Kenshi Yamasaki ◽  
Yashushi Hanakawa ◽  
Yuji Shirakata ◽  
...  
Keyword(s):  
Human Keratinocytes ◽  
Ultraviolet B ◽  
Apoptosis Inducer ◽  
Induced Apoptosis

scholarly journals Circadian Expression of TIMP3 Is Disrupted by UVB Irradiation and Recovered by Green Tea Extracts

2019 ◽  
Vol 20 (4) ◽  
pp. 862 ◽  
Author(s):  
Sunyoung Park ◽  
Eun-Soo Lee ◽  
Nok-Hyun Park ◽  
Kyeonghwan Hwang ◽  
Eun-Gyung Cho
Keyword(s):  
Clock Gene ◽  
Regulatory Gene ◽  
Human Keratinocytes ◽  
Ultraviolet B ◽  
Rhythmic Expression ◽  
The Core ◽  
Circadian Expression ◽  
Tnf Α ◽  
Factor Α ◽  
Core Clock Gene

The human skin is the outermost physical barrier and has its own circadian machinery that works either cooperatively with the central clock, or autonomously. Circadian rhythms have been observed in many functions related to epidermal homeostasis including hydration and inflammation, and this functional oscillation is disturbed by ultraviolet radiation (UVR), which is a strong environmental cue. Among the genes estimated to show circadian expression in the skin, metalloproteinase inhibitor 3 (TIMP3), has a rhythmic expression in synchronized human keratinocytes similar to that of the core clock gene PER1 and an epidermal circadian regulatory gene, aquaporin 3 (AQP3) but was antiphase to the core clock gene BMAL1. Tumor necrosis factor-α (TNF-α), the regulatory target of TIMP3 via a disintegrin and metalloproteinase domain 17 (ADAM17), was inversely regulated when TIMP3 expression was downregulated by ultraviolet B (UVB) treatment. When synthetic TIMP3 peptides were applied to the cells, the secretion of TNF-α did not increase following the UVB treatment. Similar to TIMP3 peptides, Camellia sinensis leaf-derived extracts showed a distinguishing efficacy in recovering TIMP3 expression, downregulated by UVB treatment. Together, our results suggest that TIMP3 reversely mediates UVR-induced inflammation by being highly expressed during the daytime; therefore, recovering the circadian expression of TIMP3 using synthetic TIMP3 peptides or bioactive natural ingredients could at least in part inhibit the UVR-induced cellular phenomena.

scholarly journals Nutrients Bioaccessibility and Anti-inflammatory Features of Fermented Bee Pollen: A Comprehensive Investigation

2021 ◽  
Vol 12 ◽  
Author(s):  
Pasquale Filannino ◽  
Raffaella Di Cagno ◽  
Olimpia Vincentini ◽  
Daniela Pinto ◽  
Andrea Polo ◽  
...  
Keyword(s):  
Colon Adenocarcinoma ◽  
Human Keratinocytes ◽  
Protective Role ◽  
Protective Effects ◽  
Adenocarcinoma Cell Line ◽  
Inflammatory Stimuli ◽  
Keratinocyte Cell ◽  
Functional Features ◽  
Positive Effect

We compared raw bee-collected pollen (Raw-BCP), spontaneously fermented BCP (Unstarted-BCP), and BCP fermented with selected microbial starters (Started-BCP) to deepen whether fermentation may favorably affect the nutrients bioaccessibility and functional features of BCP. Under in vitro gastrointestinal batches, the highest serum-availability of phenolic compounds was found in Started-BCP, highlighting the positive effect exerted by selected microbial starters. The same effect was not found in spontaneously fermented BCP. In colon adenocarcinoma cell line-2 (Caco-2) cells stressed by a pro-inflammatory stimulus, the treatment with Started-BCP halted the increase of pro-inflammatory mediator’s level. Started-BCP counteracted efficiently the deleterious effects of inflammatory stimuli on the integrity of the Caco-2 cells monolayer and its barrier function. Started-BCP successfully counteracted the H2O2-induced intracellular accumulation of reactive oxygen species (ROS) in Caco-2 cells. A protective role against lipopolysaccharide (LPS)-induced inflammation was exerted by Started-BCP in human keratinocytes. The same protective effects on Caco-2 and keratinocyte cell lines were negligible after treatments with Raw-BCP or Unstarted-BCP.

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