Abstract
For successful endodontic treatment, the antimicrobial property of endodontic filling is mandatory. Hence, the aim of this in vitro study was to assess the antibacterial efficacy of three endodontic sealers on Enterococcus faecalis, Candida albicans and Staphylococcus aureus by direct contact test (DCT).Materials and MethodsThe antimicrobial efficacy of three different sealers, AH Plus, MTA Fillapex and Endo Sequence BC, was tested against Enterococcus faecalis ATCC 29212, Candida albicans ATCC 10231 and Staphylococcus aureus ATCC 25923 by DCT. Freshly mixed sealers were placed in flat bottom test tubes incubated at 37°C. Thereafter, 10 μL of suspension was drawn and spread over culture plates to determine the colony count using a digital colony counter. Readings were taken at 1 hr (fresh specimen), and then at 24 hrs (after setting). The results were tabulated, and statistical analysis was performed using one-way ANOVA and Tukey’s HSD test.ResultsDCT showed a significant decrease in microbial count in AH Plus, MTA Fillapex and Endoseq BC at both time intervals. Group 3 (Endosequence BC) showed minimum microbial count followed by Group 2 (MTA Fillapex) and maximum microbial count for Group 1 (AH Plus) for both time intervals (1 hour and 24 hours).ConclusionEndosequence BC showed maximum antimicrobial efficacy against all the tested microorganisms for both time intervals, followed by MTA Fillapex and AH Plus.
Detection of Vancomycin Resistance among Enterococcus faecalis and Staphylococcus aureus
