scholarly journals MECHANISM OF CHLORAMPHENICOL RESISTANCE IN E. COLI III. THE TOTAL AMINO-ACID COMPOSITION OF CHLORAMPHENICOL RESISTANT E. COLI AND ELECTROPHORETICAL PATTERN OF ITS ^|^beta;-GALACTOSIDASE

1959 ◽  
Vol 12 (3) ◽  
pp. 125-131 ◽  
Author(s):  
SUYEHIKO OKAMOTO ◽  
KENYA OHTAKI ◽  
DEN'ICHI MIZUNO
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Total Amino Acid ◽  
Chloramphenicol Resistance ◽  
E Coli ◽  
Beta Galactosidase

Isolation of Labeled Lipoprotein from Escherichia coli and Proteus mirabilis after Incubation with [14C]Penicillin

1985 ◽  
Vol 40 (5-6) ◽  
pp. 415-420 ◽  
Author(s):  
Gerhard Gruner ◽  
Monier H. Tadros ◽  
Roland Plapp
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Proteus Mirabilis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Apparent Molecular Weight ◽  
Penicillin G ◽  
Free Form ◽  
E Coli

Abstract [14C]penicillin binding experiments and membrane analysis were carried out with cell envelope preparations from Escherichia coli and Proteus mirabilis. After incubation with [14C] penicillin G labeled free lipoprotein could be identified. The analysis of the isolated lipoprotein by SDS polyacrylamide gel electrophoresis indicates that there is only one protein with an apparent molecular weight of 7000. The amino acid composition of isolated labeled free lipoprotein from E. coli was identical to the lipoprotein already found in E. coli. It is a point of interest that the amino acid composition of the isolated labeled free lipoprotein from P. mirabilis D52 differs from that found in other mutants of this strain. The free form of lipoprotein from P. mirabilis D52 is composed of 61 amino acids and has glycine, phenylalanine and proline as specific components.

Changes in the free and total amino acid composition of ripening chestnut seeds

1984 ◽  
Vol 23 (11) ◽  
pp. 2453-2456 ◽  
Author(s):  
A.M. Desmaison ◽  
M.H. Marcher ◽  
M. Tixier
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Total Amino Acid

scholarly journals AMINO ACID COMPOSITION OF VEGETATIVE ORGANS DACTYLORHIZA MACULATA (L.) SOÓ (ORCHIDACEAE)

2019 ◽  
pp. 135-143
Author(s):  
Evgeniy Nikolaevich Sechin ◽  
Oleg Anatolyevich Marakaev ◽  
Gavriil Borisovich Gavrilov
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Plant Material ◽  
Amino Acid Content ◽  
Total Content ◽  
Total Amino Acid ◽  
Vegetative Organs ◽  
Storage Organs

Amino acid composition of aboveground and underground vegetative organs of Dactylorhiza maculata (L.) Soó (Orchidaceae), one of the representatives of the tuberoid species of orchids growing under the natural conditions of the center of European Russia, was detected using the method of zone capillary electrophoresis. The presence of 15 amino acids in the plant material, nine of them are «essential» (lysine, phenylalanine, histidine, leucine, isoleucine, methionine, valine, threonine, tryptophan) was established. The highest total amino acid content is characteristic of the leaves, the smallest for the old (wintered) caulorrhizous tuberoids. Among the identified amino acids in the plant material of D. maculata, the maximum total content is of leucine, the minimum are of tryptophan and methionine. The vegetative organs are also rich in alanine, arginine, valine and phenylalanine. The total content of amino acids in young caulorrhizous tuberoids is 38% higher than that in old storage organs. These differences are most pronounced for arginine, which is probably due to the spare function of this amino acid, containing more than 30% nitrogen. The got data indicate the promise of further studies of the amino acid composition of D. maculata and can characterize this species as a source of medicinal valuable substances with a broad spectrum of pharmacological activity.

scholarly journals Chemical composition of the leaves of tea bush (Ocimum gratissium L.)

2015 ◽  
Vol 50 (2) ◽  
pp. 93-108
Author(s):  
EI Adeyeye ◽  
MA Oyarekua
Keyword(s):  
Fatty Acid ◽  
Amino Acid ◽  
Chemical Composition ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Essential Amino Acid ◽  
Quality Criteria ◽  
Quality Parameters ◽  
Trace Minerals ◽  
Total Amino Acid

Tea bush leaves are important in the preparation of soups. Crude fat was 5.20g/100g. High value results (g/100g): protein (28.9), carbohydrate (36.2), ash (9.40), fibre (14.1); fatty acid (4.16g/100g) with energy value (154kJ/100g). High major minerals were: Ca, Mg, K and P, high trace minerals were: Fe, Cu, Mn and Zn. Mineral ratios within nutritional standards: Na/K, Ca/P and [K/(Ca + Mg)]. In amino acid composition, highest concentrated amino acid was Glu (11.0g/100g), second was Leu (7.65g/100g), total amino acid (87.2g/100g). Essential amino acid (with His) was 42.5g/100g (48.7%). Quality parameters obtained in the results: Leu/Ile (1.59), % Cys/TSAA (42.3), P-PER (2.49) and EAAI (1.18). On quality criteria, Lys was limiting in the three criteria used. Fatty acid had values of SFA (30.9%), MUFA (5.60%), PUFA (63.5%), PUFA/SFA (2.06), n-6/n-3 (1.52), EPA/DHA (1.39). Total phospholipids (44.1mg/100g) with phosphatidylcholine (15.9), phosphatidylinostitol (14.9) predominating. Cholesterol was 0.00mg/100g, sitosterol was 26.3mg/100g (61.5%).Bangladesh J. Sci. Ind. Res. 50(2), 93-108, 2015

scholarly journals More benign random peptides in E. coli are enriched for similar amino acids as young animal but not plant genes

2020 ◽  
Author(s):  
Luke Kosinski ◽  
Nathan Raul Aviles ◽  
Kevin Gomez ◽  
Joanna Masel
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
De Novo ◽  
Young Animal ◽  
Structural Disorder ◽  
E Coli ◽  
Random Peptide ◽  
Almost All

Proteins are the workhorses of the cell, yet they carry great potential for harm via misfolding and aggregation. Despite the dangers, proteins are sometimes born de novo from non-coding DNA. Proteins are more likely to be born from non-coding regions that produce peptides that do little to no harm when translated than from regions that produce harmful peptides. To investigate which newborn proteins are most likely to "first, do no harm", we estimate fitnesses from an experiment that competed Escherichia coli lineages that each expressed a unique random peptide. A variety of peptide metrics significantly predict lineage fitness, but almost all this predictive power stems from simple amino acid composition. Amino acids that are smaller and that promote intrinsic structural disorder have more benign fitness effects. Our amino acid composition-based predictions also have validity for an independent dataset using small random N-terminal tags. The same amino acids that predict high fitness in E. coli are enriched in young Pfams in animals, but not in plants. To modify Jacques Monod's famous quote, what makes peptides benign in E.coli also makes them benign in elephants, but not in eucalyptus.

ERRATUM

PEDIATRICS ◽  
1954 ◽  
Vol 14 (3) ◽  
pp. 284-284
Keyword(s):  
Amino Acid ◽  
Ion Exchange ◽  
Human Milk ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Methionine Sulfoxide ◽  
Total Amino Acid ◽  
A Value ◽  
Single Substance

Volume 12, Oct. 1953, No. 4, page 354, "Total Amino Acid Composition in Mature Human Milk" by Mung W. Cheung, M.D., Edward L. Pratt, M.D., and Dorothy I. Fowler, B.Sc. The value for methionine in our original paper does not include its oxidized product, methionine sulfoxide. In our chromatograms, this compound appears as a skewed peak, suggesting that it contains more than a single substance. However, if this possibility is disregarded and the methionine sulfoxide calculated as methionine, Pool A would have a value for methionine of 19.4 instead of 15.4 mg./100 cc. milk; and Pool B a value of 18.1 instead of 14.3 mg./100 cc. milk. The average total value for methionine of 18.8 mg./100 cc. milk is thus closer to those reported by P. Soupart, Stanford Moore, and E. J. Bigwood, J. Biol. Chem. 206:699, 1954, for human milk analyzed by the ion exchange technic which we employed. [SEE TABLE I IN SOURCE PDF].

scholarly journals Effect of Growing Seasons, Locations and Planting Dates on Total Amino Acid Composition of Two Valencia Peanut Varieties Grown in New Mexico1

1981 ◽  
Vol 8 (2) ◽  
pp. 131-133 ◽  
Author(s):  
David C. H. Hsi ◽  
Clyde T. Young ◽  
Melchor Ortiz
Keyword(s):  
Amino Acids ◽  
New Mexico ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Acid Content ◽  
Amino Acid Content ◽  
Planting Date ◽  
Total Amino Acid

Abstract Two Valencia peanut (Arachis hypogaea L.) cultivars, New Mexico Valencia A and New Mexico Valencia C, were grown at Arch and Los Lunas, N. M. All peanuts were grown under either sprinkler or furrow irrigation. Samples were obtained following harvest, hydrolyzed and analyzed in Raleigh, N. C. for amino acid content. With samples from Arch, no variety by planting date effects were noted for amino acid composition. With samples from Los Lunas, however, significant variety by planting date effects were noted for glutamic acid, glycine, methionine, isoleucine, and leucine. Planting date effects were found only for glycine at the Los Lunas location and phenylalanine at both locations. Significant variety differences were found for methionine, tyrosine, phenylalanine, and lysine at the Arch location and for glycine, methionine, phenylalanine, and lysine at the Los Lunas location. A significant year effect, although small, was noted for about half of the amino acids (aspartic acid, serine, glutamic acid, proline, glycine, tyrosine, phenylalanine, arginine, and the sum of all amino acids) studied at the Arch location. Data at Los Lunas were observed only during the second year. The amino acid content agrees closely with that previously reported for other types of peanuts except for a 100% higher level of cystine found in this study.

scholarly journals Comparative assessment of amino acids composition in two types of marine fish silage

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mukund Gauthankar ◽  
Rakhee Khandeparker ◽  
Mamatha S. Shivaramu ◽  
Komal Salkar ◽  
Rayadurga Anantha Sreepada ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Fat Content ◽  
Comparative Assessment ◽  
Total Amino Acid ◽  
Lean Fish ◽  
Amino Acids Composition ◽  
High Concentrations

AbstractFish silage is a brown liquefied product achieved by the action of enzymes when finely grounded whole/parts of either single or mixed fish types are subjected to acidification. This study made a comparative assessment of biochemical and nutritive properties, especially the amino acid composition in supernatant phase of formic acid silages prepared from two fish types, Indian mackerel (Rastrelliger kanagurta) and false travely (Lactarius lactarius) representing fat fish (FF, fat content > 5%) and lean fish (LF, fat content < 5%), respectively during 35 days of fermentation (DoF). Significantly higher content of total amino acid (TAA) and free amino acids (FAA) were recorded in FFS (TAA, 41.2 ± 0.03 mg/g; FAA, 31.3 ± 0.003 mg/g) compared to LFS (TAA, 35.8 ± 0.07 mg/g; FAA, 18.26 ± 0.003 mg/g; FAA, 31.3 ± 0.003 mg/g) (p < 0.05). At the end of 35 DoF, the concentrations of amino acids such as asparagine, histidine, isoleucine, valine, cysteine, serine, lysine and arginine were significantly higher in FFS as compared to LFS. The relative amino acid composition of FFS and LFS varied in accordance with DoF and the relationship was found to be highly significant (ANOVA, p < 0.00001). High concentrations of l-amino acids such as leucine, glutamic acid and arginine were recorded in both FFS and LFS. In conclusion, the analysis suggested that a fermentation period of 25–30 days showed a significant effect on the composition of amino acids in both types of ensilage compared to other fermentation periods (p < 0.05). Considering the role of amino acids in enhancing the plant growth and proliferation, the findings of the present study are quite useful.

scholarly journals Protein Content and Amino Acid Balance of Yams

1969 ◽  
Vol 57 (1) ◽  
pp. 78-83
Author(s):  
F. W. Martin ◽  
A. E. Thompson
Keyword(s):  
Amino Acids ◽  
High Temperature ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Protein Content ◽  
Acid Composition ◽  
Total Protein ◽  
Total Amino Acid ◽  
Amino Acid Contents ◽  
Amino Acid Balance

The total protein of 38 yam (Dioscorea) cultivars, as tested by Kjeldahl methods, and their amino acid composition, determined by high temperature hydrolysis and GLC chromatography, are reported. Species and varieties differed in total protein and total amino acid contents. Proteins of four species, but not D. alata, were somewhat low in lysine. Proteins of all species were deficient in sulphur-containing amino acids, methionine, and especially cystine. Varietal differences, however, suggest that cultivars can be selected with more balanced protein.

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