MANGROVE LEAF TISSUE SODIUM AND POTASSIUM ION CONCENTRATIONS AS SUBLETHAL INDICATORS OF OIL STRESS IN MANGROVE TREES
ABSTRACT As part of an ongoing study of the long-term effects of the Zoe Colocotroni oil spill of March 17, 1973, in Bahia Sucia, Puerto Rico, the effects of the remaining oil on the red mangrove trees in the impact area were investigated. This study involved four sampling trips to the spill site and a reference area outside the spill zone between April 1979 and April 1981. The present study was based on the observation that stressed mangrove trees in the heavily oil-affected areas had a similar appearance to trees exhibiting stress due to hypersalinity in unoiled areas. The working hypothesis was that petroleum hydrocarbons induce stress in salt-excluding plants such as red mangroves by disrupting the ability of the roots to exclude ions from seawater. Measurements of sodium (the principal seawater cation) and potassium (a major physiological ion) were made on leaf samples taken from trees from oil-affected areas and reference areas. Sediment core samples were taken from the root zone of the trees sampled at each site and analyzed for hydrocarbons by gravimetric and gas chromatographic methods. The results show a relationship between sediment hydrocarbon concentration and the ratio of sodium to potassium for mangrove leaves sampled at each site. The results show that for trees exposed at the root zone to the least weathered oil, the values of this ratio for the leaves had the largest values, reflecting an oil-induced impairment of the salt (Na) exclusion mechanism. This approach to measuring the physiological health of mangrove trees at an oil spill site offers a potentially useful means of documenting oil stress and recovery from oil stress in salt-excluding halophytes. Because oil stress in mangroves appears to be a root membrane-directed effect, there may be a “window in time” between initial oil impact and plant damage, as oil penetrates the sediments, during which mitigation measures could be taken.