Phytochemical Screening and Evaluation of in-vitro Anti-oxidant Activity of Extracts of Ixora javanica D. C Flowers

2016 ◽  
Vol 10 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Sunitha Dontha ◽  
Hemalatha Kamurthy ◽  
Bhagavan Manthripragada
Keyword(s):  
Phytochemical Screening ◽  
Anti Oxidant

scholarly journals In vitro thrombolytic, anthelmintic, anti-oxidant and cytotoxic activity with phytochemical screening of methanolic extract of Xanthium indicum leaves

2015 ◽  
Vol 10 (4) ◽  
pp. 854 ◽  
Author(s):  
Antara Ghosh ◽  
Sujan Banik ◽  
Md. Ariful Islam
Keyword(s):  
Cytotoxic Activity ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Anthelmintic Activity ◽  
Positive Control ◽  
Clot Lysis ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Anti Oxidant

<p class="Abstract"><em>Xanthium indicum</em> is an important medicinal plant traditionally used in Bangladesh as a folkloric treatment. The current study was undertaken to evaluate thrombolytic, anthelmintic, anti-oxidant, cytotoxic properties with phytochemical screening of methanolic extract of <em>X. indicum</em> leaves. The analysis of phytochemical screening confirmed the existence of phytosetrols and diterpenes. In thrombolytic assay, a significant clot lysis was observed at four concentrations of plant extract compare to the positive control streptokinase (30,000 IU, 15,000 IU) and negative control normal saline. The extract revealed potent anthelmintic activity at different concentrations. In anti-oxidant activity evaluation by two potential experiments namely total phenolic content determination and free radical scavenging assay by 2, 2-diphenylpicrylhydrazyl (DPPH), the leaves extract possess good anti-oxidant property. In the brine shrimp lethality bioassay, the crude extract showed potent (LC<sub>50</sub> 1.3 μg/mL) cytotoxic activity compare to the vincristine sulfate as a positive control (LC<sub>50</sub> 0.8 μg/mL). </p><p> </p>

Phytochemical Screening and In Vitro Anti-Oxidant Activity of Alternanthera Pungens

2018 ◽  
Vol 01 (01) ◽  
Author(s):  
M. Srikanth ◽  
P. Sowmya ◽  
K. Ramanjaneyulu ◽  
J. Himabindhu
Keyword(s):  
Phytochemical Screening ◽  
Anti Oxidant

scholarly journals Qualitative Phytochemical Screening, GCMS Studies and In-Vitro Anti-Oxidative Properties of Aqeuous Leaf Extract of Gnetum africanum

2020 ◽  
Vol 10 (1) ◽  
pp. 11-17
Author(s):  
A.S. Ezekwe ◽  
P.C. Ugwuezumba ◽  
P. Nwankpa ◽  
J.N. Egwurugwu ◽  
C.N. Ekweogu ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Antioxidant Properties ◽  
Antioxidant Property ◽  
Phytochemical Screening ◽  
Standard Drug ◽  
Standard Procedures ◽  
Anti Oxidant ◽  
Aqueous Leaf Extract ◽  
Dpph Scavenging

This study aimed at carrying out a qualitative phytochemical screening, GC-MS studies and in-vitro antioxidant properties of aqueous leaf extract of Gnetum africanum. The qualitative phytochemical screening of the aqueous leaf extract of Gnetum africanum was done using standard procedures and revealed the presence of terpenoids, saponins, tannins, steroids, flavonoids, alkaloids, cardiac glucosides and phenols. The GC-MS screening revealed the presence of 14 compounds, 6 out of the 14 compounds were most prominent. The compound with the highest percentage peak area was caffeine with peak area of 96.9%, followed by n-Hexadacanotic acid with peak area of 60.9%, 2-methoxy-4-vinylphenol with peak area of 55.9%, tetradacanoic acid with peak area of 50.3%, cyclopentaneundecanoic acid with peak area of 47.8% and 2-cyclo-penten-1-2-hydroxy with peak area of 43.6% respectively. In-vitro determination of antioxidant property of leaf extract of Gnetum africanum was done photometrically using 2,2-dyhenyl-l-picrylhydrazyl (DPPH) assay. The DPPH scavenging ability of the leaf extract (43.2, 60.5, 68.8, and 75.7) was statistically significant at p<0.05 when compared with the standard drug ascorbic acid (81.1, 82.6, 85.1, and 90.4) % at 10, 20, 30 and 40 mg/l. In conclusion, the leaf extract of Gnetum africanum is loaded with a host of important phytochemicals and has antioxidant properties which increase in potency with increase dose. Keywords: Phytochemical Screening, GCMS Studies, Anti-Oxidant, Gnetum africanum

scholarly journals Phytochemical screening & in-vitro evaluation of anti-inflammatory activity of clerodendrum indicum roots

2021 ◽  
pp. 140-143
Author(s):  
Sushma M ◽  
Lahari S ◽  
Mounika A ◽  
Sailaja K.E
Keyword(s):  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Phytochemical Screening ◽  
In Vitro Evaluation ◽  
Anti Cancer ◽  
The Family ◽  
Steroid Glycosides ◽  
Anti Oxidant ◽  
Anti Inflammatory

This study aims to extract the phytochemical constituents and in-vitro evaluation of   anti-inflammatory activity of roots of clerodendrum Indicum. Clerodendrum indicum (L.) kuntz. Commonly known as bharangi or chingari, belongs to the family verbenaceae. The plant is expectorant, stomachic, Anti-bronchitis, Anti-nociceptive, Anti-microbial, Anti-diarrheal, anti-oxidant and anti-cancer. Preliminary phytochemical screening revealed the presence of flavonoids, steroids, steroid glycosides, terpenoids etc. This study has taken with the Anti-inflammatory  activity and preliminary phytochemical screening on the clerodendrum indicum. In the present study, qualitative study of    the phytochemicals present in the sample was determined, Anti-inflammatory  activity  were carried out. On the premise of the prevailing study, the outcomes eventually concluded that the ethanolic extract of clerodendrum indicum own anti inflammatory pastime. This evaluation offers an concept that the compound of the plant clerodendrum indicum may be used as lead compound for designing robust pills which may be used for remedy of diverse diseases.

Protective Effect of Vitamin E on Immune Triggered, Granulocyte Mediated Endothelial Injury

1984 ◽  
Vol 51 (01) ◽  
pp. 089-092 ◽  
Author(s):  
M A Boogaerts ◽  
J Van de Broeck ◽  
H Deckmyn ◽  
C Roelant ◽  
J Vermylen ◽  
...  
Keyword(s):  
Vitamin E ◽  
Protective Effect ◽  
Umbilical Vein ◽  
Endothelial Damage ◽  
Endothelial Injury ◽  
Mediated Effects ◽  
Anti Oxidant ◽  
Endothelial Cell Cultures ◽  
Vitro Model

SummaryThe effect of alfa-tocopherol on the cell-cell interactions at the vessel wall were studied, using an in vitro model of human umbilical vein endothelial cell cultures (HUEC). Immune triggered granulocytes (PMN) will adhere to and damage HUEC and platelets enhance this PMN mediated endothelial injury. When HUEC are cultured in the presence of vitamin E, 51Cr-leakage induced by complement stimulated PMN is significantly decreased and the enhanced cytotoxicity by platelets is completely abolished (p <0.001).The inhibition of PMN induced endothelial injury is directly correlated to a diminished adherence of PMN to vitamin E- cultured HUEC (p <0.001), which may be mediated by an increase of both basal and stimulated endogenous prostacyclin (PGI2) from alfa-tocopherol-treated HUEC (p <0.025). The vitamin E-effect is abolished by incubation of HUEC with the irreversible cyclo-oxygenase inhibitor, acetylsalicylic acid, but the addition of exogenous PGI2 could not reproduce the vitamin E-mediated effects.We conclude that vitamin E exerts a protective effect on immune triggered endothelial damage, partly by increasing the endogenous anti-oxidant potential, partly by modulating intrinsic endothelial prostaglandin production. The failure to reproduce vitamin E-protection by exogenously added PGI2 may suggest additional, not yet elucidated vitamin E-effects on endothelial metabolism.

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