scholarly journals Genetic Variations in Haematological Indices of Local and Exotic Turkeys Inoculated with Attenuated Salmonella typhimurium Vaccine

Author(s):  
Oluwafemi David Oguntade ◽  
Babatunde Moses Ilori ◽  
Olutunde Samuel Durosaro ◽  
Adesanya John Abiona ◽  
Clement Ebanehitah Isidahomen ◽  
...  

Infection due to Salmonella spp is one of the threatening diseases to poultry industry because it can cause economic losses through mortality, morbidity and reduction in egg production. One hundred and sixty poults comprising 80 local and 80 exotic (Nicholas white) were used to examine the variations in haematological response to Salmonella spp in local and exotic turkeys inoculated with attenuated Salmonella spp. Both genotypes were reared separately on deep litter, under the same management practices and wing tagged for proper identification. At 7th week of age, 1ml of blood sample was collected from each turkey into anticoagulant tubes and labeled accordingly for base-line haematological analysis. The turkeys were inoculated at 8th week by subcutaneous injection of attenuated Salmonella vaccine at 0.5ml per turkey. On the 2nd and 7th days after inoculation, 1ml of blood samples was collected from each turkey for haematological analyses. The un-coagulated blood was used to determine packed cell volume (PCV), red blood cell (RBC), white blood cell (WBC) and its differential counts, and haemoglobin (Hb). The mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were calculated. The haematological data collected were analyzed using General Linear Model of SAS 9.0 software. The exotic turkey had higher (p<0.05) values of PCV (38.13±1.01), Hb (12.77±0.33gd/l), MCV (133.39±0.14fL) and MCH (43.35±1.27pg/cell) before inoculation. On 2nd day post inoculation, the local turkey had higher (P<0.05) values of PCV (43.93±1.40%), RBC (3.98±0.12 106µl-1) and Hb (14.56±0.47gd/l). Also, on the seventh day post inoculation, the local turkey had higher (P<0.05) values of PCV (42.13±1.20%), WBC (11.94±0.62 103.µ-1), Hb (14.09±0.38 gd/l) and MCH (40.65±0.34pg/cell). The mean values of PCV, Hb, MCV, MCH and MCHC of local turkeys were higher (p<0.05) on the 7th day than on the 2nd day post inoculation, while the RBC was higher (P<0.05) on the 2nd day post inoculation. The WBC, MCV and MCH values were higher (p<0.05) in exotic turkey on the 2nd day post inoculation. The local turkey showed quick haematological response to Salmonella vaccine especially post inoculation. Therefore, the local turkey could be said to have stronger potential to survive under disease prevailing environment than the exotic turkey.

2010 ◽  
Vol 79 (9) ◽  
pp. S3-S8 ◽  
Author(s):  
Eva Straková ◽  
Pavel Suchý ◽  
Romana Kábelová ◽  
František Vitula ◽  
Ivan Herzig

The present paper extends the knowledge of selected blood indicators in six species of feathered game such as helmeted guineafowl (Numida meleagris), common pheasant (Phasianus colchicus), Japanese quail (Coturnix coturnix japonica), chukar (Alectoris chucar), grey partridge (Perdix perdix) and wild turkey (Meleagris gallopavo), reared in the Czech Republic and Europe. The red blood cell (RBC) values in individual species of feathered game ranged as follows: the mean RBC counts varied in a range of 2.10 - 2.58 T/l; haematocrit values were in a range of 0.33 - 0.39 1/1; haemoglobin concentration varied in a range of 101.20 - 129.13 g/l; mean corpuscular haemoglobin concentration (MCHC) ranged from 28.63 to 35.44%; mean corpuscular haemoglobin (MCH) varied in a range of 46.15 - 62.07 pg and the mean corpuscular volume (MCV) was 147.66 - 178.20 fl. Except for the total concentration of haemoglobin and the mean corpuscular haemoglobin concentration, no significant differences were found between the mean values of RBC values in individual species of feathered game. However, some differences between species were found in white blood cell (WBC) values, ranging as follows: the total WBC count ranged from 13.55 to 26.90 G/l, basophil granulocytes from 0.011 to 0.110 G/l, eosinophil granulocytes from 0.011 to 0.593 G/l, neutrophil granulocytes from 1.868 to 6.661 G/l, lymphocytes from 10.937 to 20.922 G/l and monocytes from 0.034 to 0.199 G/l. Most values showed significant (P ⪬ 0.05) to highly significant (P ⪬ 0.01) interspecies differences. The main goal of the study was to investigate selected haematological indicators in six major species of feathered game.


2018 ◽  
pp. 53-57

Introduction: Anaplasma ovis is an intraerythrocytic rickettsial pathogen that causes clinical symptoms and huge economic losses in flocks. This study was designed to determine the prevalence and effects of Anaplasma ovis on the haematology of apparently healthy Sahel goats in Maiduguri. Methods: Blood samples were randomly collected from one hundred (100) Sahel goats from both farm and abattoir in the study area. Parasitological and haematological analyses were carried out on the collected samples using a standard technique. Results: Out of 100 goats sampled, seven (7) were positive with an overall prevalence of 7%. The prevalence was higher in the male 5 (5%) than in the female 2 (2%) with no significant association between sexes (p > 0.05). The haematological parameters of infected and uninfected goats were within normal range except for mean corpuscular volume (MCV) of infected goats. The MCV of infected and uninfected goats varied significantly (p < 0.05) while the packed cell volume, haemoglobin concentration, red blood cell count, white blood cell count, mean corpuscular haemoglobin concentration and mean corpuscular haemoglobin of infected and uninfected goats were not significantly (p > 0.05) affected. Significance: Natural infection in apparently healthy goats in Maiduguri was found not to have an obvious effect on the haematology of the infected animals. There is a need for prevention and control programs against this pathogen in the study area. This, when carried out, will improve the production potential of these animals and economic well-being of the owners.


2020 ◽  
Vol 6 (3) ◽  
pp. 276-286
Author(s):  
OE Ojewole ◽  
U Abubakar ◽  
AO Olowu

Background: Haematological parameters are used as screening tools for patient’s care and treatment. Objective: To determine the haematological parameters of apparently healthy term Nigerian babies and generate normative data. Methods: This was a descriptive, longitudinal study of consecutively recruited babies. The haematological parameters were determined using venous blood samples drawn at delivery and on the 14th day of life using a haematology auto-analyser. Results: Of the 309 babies recruited on the first day of life, 160 (51.8%) were males while 149 (48.2%) were females. Two hundred and seventy-nine (90.3%) were delivered vaginally.  Most (75%) of the neonates were of Nupe origin. The mean haematocrit, haemoglobin concentration, Mean Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin (MCH), Mean Corpuscular Haemoglobin Concentration (MCHC), Red Blood Cell and reticulocyte count on Day 1 were 48.8±8.0%, 15.0±2.6g/dl, 104.1±8.8fl, 31.9±3.3pg, 30.7±1.7g/dl, 4.7±0.9 (×109/l) and 2.0±1.4% respectively. Corresponding Day 14 values were 41.2±5.2%, 12.8±1.9g/dl, 97.2±9.8fl, 30.3±3.8pg, 31.1±1.7g/dl, 4.5±0.6 (×109/l) and 1.0±0.7% respectively. The mean White Blood Cell (WBC) and platelet counts were 13.3±4.8 (×109/l) and 228.3±81.2 (×109/l) respectively, on the 1st day of life and 10.1±2.4 (×109/l) and 370.1±120.7 (×109/l) respectively on the 14th day. The mean differential WBC counts were 49.4±12.0% for neutrophils and 37.4±11.3% for lymphocytes on the 1st day and 32.4±10.0% and 52.8±9.3% respectively on the 14th day. Conclusion: Neonates from Bida have haematological parameters comparable with previously reported values from other parts of Nigeria.


2018 ◽  
Vol 3 (1) ◽  
pp. 10-16
Author(s):  
George C. Ugwu ◽  
Olufemi O. Soyinka

The objective of the present study was to examine the haematological and biochemical parameters of the silver catfish, Chrysichthys nigrodigitatus, from the Makoko area of a polluted tropical lagoon such as Lagos Lagoon, to serve as a baseline data for the assessment of the health status of the fish and as reference point for future studies. The mean values of the haematological parameters analyzed were: blood haemoglobin, Hb (97.29±4.35g/L); pack cell volume, PCV (29.28±1.23%); red blood cell/total erythrocyte count, RBC (2.97±0.12T/L); white blood cell count, WBC (10.69±0.37g/L); mean cell volume, MCV (95.19±2.28fl); mean corpuscular haemoglobin, MCH (30.53±0.67pg), mean corpuscular haemoglobin concentration, MCHC (33.32±0.38g/dl), and the differential leucocytes. While the mean values of the biochemical parameters were: aspartate transaminase, AST (66.06±6.12 IU/L); alanine transaminase, ALT (12.98±1.10 IU/L), and alkaline phosphatase, ALP (88.01±8.60 IU/L). The findings from this study gave an indication of stress on the health status of the fishes in this lagoon and there is need for proper management strategies to be adopted in monitoring the conditions of the faunal communities in the lagoon. The lagoon indeed showed signs of environmental stress, which eventually pose devastating effect on the health status of the fishes. These have an indirect effect on the coastal communities. There is therefore the need for periodic monitoring and enforcement of environmental laws by respective organisations to ensure health safety of the people especially in the coastal communities.


2017 ◽  
Vol 1 (1) ◽  
pp. 29-43
Author(s):  
Muhammad Abdul Ghaffar ◽  
Muhammad Asif Naveed ◽  
Rehma Dar

Background: The complete blood count is one of the most common routine tests. This study aimed to evaluate possible effects of the antioxidant taurine on the complete blood count of whole blood stored at room temperature and at 4°C over three days.Methods: Venous blood samples of 15 healthy males were distributed into two sets of tubes with each set of three tubes containing 50 μL of solutions with zero, 5 g/L, 10 g/L taurine. The tubes were kept at room temperature or at 4°C. Complete blood counts were performed on three successive days. The mean percentage changes [Δ = (mean value – mean baseline value) / mean baseline value x 100] were calculated and compared.Results: Complete blood count parameters exhibited different patterns of behaviour which were affected by the storage temperature, time and taurine concentration. Taurine at room temperature significantly enhanced the stability of: the platelet count over three days (Δ3 at 5 and 10 g/L taurine were 6.18, and 2.53 x 109 cells/L, respectively); the red blood cell count over three days (Δ3 at 5 and 10 g/L taurine were 2.59, and 1.39 x 1012 cells/L, respectively); mean corpuscular haemoglobin over three days (Δ3 at 5 and 10 g/L taurine were,-0.62 and -0.52 fl respectively); and red cell distribution width over two days (Δ2 at 5 and 10 g/L taurine were 1.30% and -0.1%, respectively). No additional stabilizing effects of taurine were reported for the mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, haematocrit and haemoglobin, while it negatively affected the white blood cell stability.Conclusion: Complete blood count parameters exhibited variable stability patterns in respect to temperature, time and taurine concentration.


2011 ◽  
Vol 3 (4) ◽  
pp. 29-34 ◽  
Author(s):  
Ovie KORI-SAKPERE ◽  
Urowoli IKOMI

The haematological alterations produced on exposure of the African snakehead, Parachanna africans to the sublethal concentration (0.0, 0.1, 1.0 and 10.0 mg/L) of cadmium (Cd2+) for 21 days have been studied. Red blood cell (RBC) count, haemoglobin (Hb) concentration, haematocrit (Hct), the mean corpuscular haemoglobin (MCH) and the mean corpuscular volume (MCV) levels were decreased with an increase in exposure concentration, but the level of the mean corpuscular haemoglobin concentration (MCHC) was increased. These alterations could be attributed to haemolysis and impairment of haemoglobin synthesis, resulting in a hypochromic microcytic anaemia, induced by exposure to cadmium. Cadmium exposure also caused significant decreases in white blood cell (WBC) count. The primary consequence of the observed changes in total and differential leucocyte counts in stressed fish was attributed to suppression of the immune system and increased susceptibility to disease. Plasma glucose and total plasma protein concentrations were significantly decreased; showing that cadmium affects the fish energy metabolism. The present study thus confirmed that haematological parameters are very sensitive indicators of fish organism response to chemicals in this case cadmium.


2021 ◽  
Author(s):  
OLuwatosin Christie Raimi ◽  
Abiodun Adefunmilayo Adeloye

Abstract This study investigated the effects of feeding ensiled graded combinations of cocoa pod, cassava pulp and Acacia leaf on haematological and serum biochemical of West African Dwarf goats. In the bid to control environmental pollution, the wastes of cocoa and cassava were applied as alternative feed ingredients for the goat in spite of its low nutrients’ quality and digestibility. A total of Twenty-eight (28) WAD goats (bucks), aged 4 to 5 months with average body weight of 7.00±0.2kg was used in a completely randomized design for a 45 days study to evaluate the blood profiles of goats fed diet combinations (0:60:40) to (60:0:40). The results revealed significant differences in some of the blood parameters measured among the treatments. The red blood cell, packed cell volume and haemoglobin ranged from 4.90-7.87x106/ul, 19.25-26.83% and 7.33-11.15g/L respectively. The concentrations of mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration varied significantly (p < 0.05), white blood cell (5.57-9.75x109L) were comparable and lymphocytes (%) ranged from 43.85-55.67. For serum biochemistry, values obtained for total protein and albumin with sodium followed the same trend while cholesterol, urea, creatinine and glucose followed the same trend. Alanine amino transferase, Aspartate aminotransferase and Alkaline phosphatase varied significantly (p<0.05) among the treatment groups. Since the parameters measured were within the normal range for goats’ blood profile, combinations of cocoa pod, cassava pulp with acacia leaves in the ratio of 0:60:40 to 20:40:40 as browse plants do not have any deleterious effect on the health of the animals.


2021 ◽  
Vol 8 (27) ◽  
pp. 2434-2438
Author(s):  
Siddegowda M.S ◽  
Chaithra R ◽  
Shivakumar S ◽  
Maithri C.M

BACKGROUND Thyroid hormones play an important role in the regulation and production of red blood cells. Thyroid dysfunction induces different effects on blood cells such as anaemia, erythrocytosis, leucopenia, thrombocytopenia and alteration in red cell indices. In this study, we wanted to compare the changes in haematological parameters of thyroid dysfunction patients with those of euthyroid group. METHODS This was a retrospective study done on 310 individuals by collecting data from the medical records. Later the patients were categorized into hypothyroid (33) thyroid stimulating hormone (TSH > 5.5 μIU/mL), hyperthyroid (19) (TSH < 0.3 μIU/mL) and euthyroid (258) (TSH = 0.3 - 5.5 μIU/ml) groups. The haematological parameters of all these patients were obtained by 5-part automated cell count analyser. Finally, the obtained data was analyzed by statistical package for social sciences (SPSS) software. RESULTS The data obtained from the analysis revealed statistically significant (P < 0.05) difference between hypothyroidism, hyperthyroidism and euthyroidism in mean red blood cell (RBC) count, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), red cell distribution width (RDW), white blood cell (WBC) count and platelet count but the difference was not significant for mean haemoglobin, mean corpuscular haemoglobin concentration (MCHC) (P > 0.05). The mean haemoglobin was lower in hypothyroid patients when compared to euthyroid and hyperthyroid patients. The RBC count (P < 0.007), MCH (P = 0.002) and RDW (P < 0.001) showed statistically significant difference between hypothyroidism and euthyroidism, MCV (P = 0.005) showed statistically significant difference between hyperthyroid and euthyroid groups. CONCLUSIONS In case of patients with abnormal haematological parameters, thyroid hormones evaluation is necessary. KEYWORDS Hypothyroidism, Hyperthyroidism, Haemoglobin, Blood Count, Red Cell Indices


2021 ◽  
Author(s):  
Irena Golinar Oven ◽  
Alenka Nemec Svete ◽  
Melita Hajdinjak ◽  
Jan Plut ◽  
Marina Stukelj

Abstract BackgroundHaematological examination is an important diagnostic tool in the assessment of pig health status. The present study aimed to assess haematological parameters in pigs of different age categories from six farrow-to-finish farms differing in herd health status. The following pig categories were included: 5 age groups of growers (5, 7, 9–10, 11 and 12–13 weeks-old), fatteners and breeding pregnant sows. Individual blood samples for determining complete blood count and white blood cell differential count were taken and group samples of oral fluid and faeces were collected from each animal category in each of the six farms and tested for the detection of Porcine Circovirus Type 2 (PCV2), Porcine Reproductive and Respiratory Virus (PRRSV), and Hepatitis E Virus (HEV) using PCR, RT-PCR, and qRT-PCR protocols. Individual blood samples were analysed using an automated laser-based haematology analyser. The following haematological parameters were reported: white blood cell count (WBC), red blood cell count (RBC), haemoglobin concentration (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), platelet count (PLT), and percentage and number of neutrophils, lymphocytes, monocytes, eosinophils, basophils, and "large unstained cells" - LUCs. ResultsOn farms free of PRRSV, PCV2 and HEV, age had significant effect on the following parameters: WBC, Hb, MCH, MCHC, PLT, percentage of neutrophils, lymphocytes and eosinophils and absolute numbers of neutrophils, lymphocytes, monocytes, basophils and LUCs. On farms with PRRS, PCV2 and/or HEV, age significantly affected all observed blood parameters except the percentage of LUCs. The percentages of lymphocytes, MCV and Hct were significantly lower by PRRSV while WBC, PLT, percentage and absolute number of neutrophils, basophils and LUCs increased. Significantly lower percentages of lymphocytes and increased percentages and absolute numbers of neutrophils, eosinophils and basophils were caused by PCV2 presence. Significantly lower percentage of lymphocytes and MCV and increased RBC, Hb, percentage and number of basophils and percentage of neutrophils were caused by HEV. Conclusions Alterations of haematological parameters reflected the health status of pigs of different categories on infected and on non-infected farms. Age-related changes in haematological parameters occurred in clinically healthy and in infected pigs.


2021 ◽  
Vol 36 (1) ◽  
pp. 13-21
Author(s):  
A.B. Ella ◽  
E.T. Azua ◽  
C.U. Aguoru ◽  
A.A. Onekutu ◽  
F.A. Ella

The study evaluated sub-lethal toxicity of commercial glyphosate-based Di-ammonium formulation commonly known as ‘Touchdown’ on haematological profile of Clarias gariepinus juveniles. A total of two hundred catfish juveniles of mean weight 31.07±1.23g and mean length 19.50 ± 0.50cm were exposed to different  concentrations of Di-ammonium formulation (1.26, 2.52, 5.03 and 0.00 mg/l) for eight (8) weeks. Each concentration was treated in triplicate using a static bioassay system. Physico-chemical parameters of the test water were monitored throughout the study period. Results showed that haematocrit (HCT), red blood cell (RBC), haemoglobin (HGB), Mean Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin (MCH), Mean Corpuscular Haemoglobin Concentration (MCHC) and Platelets (PLT) counts decreased significantly (p<0.05) with increase in concentrations of Touchdown. Whereas, white blood cell (WBC) counts increased significantly (p<0.05) with increasing concentrations of the glyphosate-based formulation. Water temperature, pH, Total dissolved solid (TDS) and electrical conductivity (EC) increased linearly with increasing concentration, while dissolved oxygen content decreased significantly (p<0.05). The results from this study indicated that Di-ammonium glyphosate–based formulation had negative consequences on the blood of treated fish. In view of this, the application of Di-ammonium glyphosate-based formulation should be done in a sustainable manner with proper regulations.


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