scholarly journals Effects of Age and Sex on the Healing of Acetic-Acid Induced Ulcerative Colitis in Adult Wistar Rats

2021 ◽  
pp. 63-73
Author(s):  
Serah F. Ige ◽  
Waliyat O. Aremu ◽  
Bolade S. Olateju ◽  
Victor A. Oladipupo ◽  
Adedayo T. Adekola
Keyword(s):  
Ulcerative Colitis ◽  
Superoxide Dismutase ◽  
Acetic Acid ◽  
Adult Female ◽  
Adult Male ◽  
Wistar Rats ◽  
Female Rats ◽  
Male Rats ◽  
Cell Numbers ◽  
Age And Sex

Aims: Ulcerative colitis is a disease of the bowel that occurs in all ages and affects both males and females. This research study was designed to investigate the effect of age and sex on the healing of colitis in rats. Methodology: Twenty - eight rats were randomly distributed into four groups of seven animals per group; adult male rats, mid age male rats, adult female rats and mid age female rats. Mid age and adult Wistar rats were 7- 8weeks and 14 weeks old respectively. Colitis was induced through a single intra-colonic instillation of 7% acetic acid (1mL/100g body weight) and allowed to heal for 14 days. Blood samples were obtained for analysis. Colon samples were also obtained for histomorphological study and biochemical assays (Myeloperoxidase activities, Superoxide dismutase, Glutathione, Catalase and Malondialdehyde) levels. Results: There was no significant difference in Malondialdehyde concentration, catalase, Superoxide dismutase, Myeloperoxidase, Platelet Distribution Width, Platelet Count, Basophil cell numbers, Eosinophil cell numbers, platelet cells, Mean Platelet Volume , Mean Cell Volume and white blood cells across the groups. The Glutathione concentration in mid age male rats was significantly increased when compared with adult male rats. The haemoglobin , Lymphocytes and Mean Cell Haemoglobin levels were increased while neutrophils and monocyte levels were decreased in the younger female rats. The histomorphological study revealed poorly preserved surface epithelia layer of the colon in adult male rats while mid age male and female rats showed moderately preserved surface epithelia layer, adult female rats showed normal surface epithelia layer. Conclusion: Mid age rats heal faster than adult rats while in terms of sex, female rats tends to heal faster than male rats.

EFFECT OF SEX AND AGE AT GONADECTOMY ON THE SEBACEOUS RESPONSE TO PROGESTERONE

1977 ◽  
Vol 73 (1) ◽  
pp. 67-70 ◽  
Author(s):  
SAM SHUSTER ◽  
WENDY M. HINKS ◽  
A. J. THODY
Keyword(s):  
Adult Female ◽  
Adult Male ◽  
Female Rats ◽  
Male Rats ◽  
Adult Males ◽  
Adult Rat ◽  
Sebum Production ◽  
Males And Females ◽  
Sebum Secretion

SUMMARY The effect of progesterone on the rate of sebum secretion was examined in intact and gonadectomized rats. In intact, adult, male rats, progesterone administered for 3 weeks decreased sebum secretion; after castration of adult males, progesterone increased sebum secretion and an even greater response occurred in males castrated at 21 days of age. In intact, adult, female rats progesterone slightly increased sebum production. As in the male, the response was affected by the time of gonadectomy, a greater response occurring after spaying at 21 days compared with 10 weeks of age. Thus, the response to progesterone in the adult rat differs in intact males and females and is affected by changes in the endocrine environment induced by gonadectomy, especially near the time of puberty.

scholarly journals CORRELATION OF GENDER AND LEPTIN WITH ANALGESIC EFFECT OF TRAMADOL IN RATS

2018 ◽  
Vol 11 (6) ◽  
pp. 493
Author(s):  
Shakta Mani Satyam ◽  
Laxminarayana Kurady Bairy ◽  
Vasudha Devi
Keyword(s):  
Acetic Acid ◽  
Correlation Coefficient ◽  
Wistar Rats ◽  
Pearson Correlation ◽  
Female Rats ◽  
Male Rats ◽  
Control Group ◽  
Pearson Correlation Coefficient ◽  
Serum Leptin ◽  
Plantar Test

 Objective: The objective of the study was to investigate the correlation of gender and serum leptin level with analgesic modulation of tramadol in Wistar rats.Methods: A total of 48 Wistar rats (body weight 100–150 g), 24 each male and female Wistar rats were randomly divided into two groups (n=6/group) (Group I - Control- 0.9% NaCl; 1 ml/kg/day i.p. and Group II - Tramadol 10 mg/kg/day i.p.) for each nociception model - plantar test and acetic acid induced writhing test. The treatment duration was of 5 days. Paw withdrawal latency (PWL) was assessed using plantar test and writhing movements were observed following administration of 0.8% acetic acid; 10 ml/kg i.p.Results: PWL was significantly decreased (p<0.001) and both number of writhing movements and serum leptin concentrations were significantly increased (p<0.001) in female control group compared to male control group. In tramadol treated female rats, PWL was significantly decreased (p=0.005) and both number of writhing movements and serum leptin concentrations were significantly increased (p<0.001) in comparison with the tramadol treated male rats. PWL was negatively correlated with serum leptin concentration (Pearson correlation coefficient= −0.826, two-tailed significance= 0.000), and writhing movements were positively correlated with serum leptin concentration (Pearson correlation coefficient= 0.505, two-tailed significance= 0.012).Conclusions: The present study revealed that female rats have more serum leptin concentration than male rats which could be one of the possible reasons for having more pain sensitivity to noxious stimuli in female rats compared to male rats. Tramadol treatment at the dose of 10 mg/kg for 5 days has decreased serum leptin level in rats which might be one of the additional mechanisms of tramadol to reduce pain.

scholarly journals Endocrine regulation of sex differences in hepatic histidase activity

1985 ◽  
Vol 231 (3) ◽  
pp. 785-787 ◽  
Author(s):  
C A Lamartiniere
Keyword(s):  
Sex Differences ◽  
Adult Female ◽  
Adult Male ◽  
Pituitary Hormones ◽  
Female Rats ◽  
Male Rats ◽  
Endocrine Regulation ◽  
Pituitary Glands

Hepatic histidase activity in adult female rats is twice that in adult male rats. Hypophysectomy and thyroidectomy result in a significant increase in hepatic histidase activities in males, but not in females. This effect on histidase is reversed by the exogenous administration of tri-iodothyronine, but not by ectopic pituitary glands or purified pituitary hormones.

scholarly journals Antidiarrheal Activity of Dissotis multiflora (Sm) Triana (Melastomataceae) Leaf Extract in Wistar Rats and Subacute Toxicity Evaluation

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Alian Désiré Afagnigni ◽  
Maximilienne Ascension Nyegue ◽  
Chantal Florentine Ndoye Foe ◽  
Youchahou Njankouo Ndam ◽  
Frédéric Nico Njayou ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Shigella Flexneri ◽  
Female Rats ◽  
Male Rats ◽  
Dependent Manner ◽  
Subacute Toxicity ◽  
Antidiarrheal Activity ◽  
Dose Dependent ◽  
Ethanolic Leaf Extract

The present work was undertaken to evaluate antidiarrheal activity of ethanolic leaf extract of Dissotis multiflora (Sm) Triana (D. multiflora) on Shigella flexneri-induced diarrhea in Wistar rats and its subacute toxicity. Diarrhea was induced by oral administration of 1.2 × 109 cells/mL S. flexneri to rats. Antidiarrheal activity was investigated in rats with the doses of 111.42 mg/kg, 222.84 mg/kg, and 445.68 mg/kg. The level of biochemical parameters was assessed and organs histology examined by 14 days’ subacute toxicity. S. flexneri stool load decreased significantly in dose-dependent manner. The level of ALT increased (p<0.05) in male rats treated with the dose of 445.68 mg/kg while creatinine level increased in rats treated with both doses. In female rats, a significant decrease (p<0.05) of the level of AST and creatinine was noted in rats treated with the dose of 222.84 mg/kg of D. multiflora. Histological exams of kidney and liver of treated rats showed architectural modifications at the dose of 445.68 mg/kg. This finding suggests that D. multiflora leaf extract is efficient against diarrhea caused by S. flexneri but the treatment with doses lower than 222.84 mg/kg is recommended while further study is required to define the exact efficient nontoxic dose.

scholarly journals Age-Related Effects on Right Femoral Bone of Male Wistar Rats: A Morphometric and Biomechanical Study

2021 ◽  
Author(s):  
Sheila Martins Puelker ◽  
Sonia Regina Ribeiro de Castro ◽  
Romeu Rodrigues de Souza ◽  
Laura Beatriz Mesiano Maifrino ◽  
Ricardo Aparecido Baptista Nucci ◽  
...  
Keyword(s):  
Cortical Thickness ◽  
Wistar Rats ◽  
Female Rats ◽  
Male Rats ◽  
Aged Rats ◽  
Femoral Bone ◽  
Middle Aged ◽  
Left Femur ◽  
Bone Stiffness ◽  
The Right

Abstract Introduction Study of the variations of bone characteristics with age in different animal models is important to design musculoskeletal studies. Thus, this study aimed to evaluate the bone mass, dimensions, and biomechanical parameters of the femur in young, middle-aged, and aged Wistar rats. Materials and Methods Thirty male rats (Rattus norvegicus) were divided in three groups (n = 10 per group)—3-month-old young rats, 12-month-old middle-aged rats, and 18-months-old aged rats. The right femurs were subjected sequentially to morphometric study (bone weight, cortical thickness) and biomechanical tests (maximum resistance strength and bone stiffness). Results We observed a significant increase in femur histological (cortical thickness) and biomechanical (maximum strength and bone stiffness) parameters with aging when compared with young animals. Conclusions With the advancing age, the right femoral bone of middle-aged and old animals had greater variations when compared with young animals. However, further studies with the aid of a comparison between right and left femur and other long bones in both male and female rats are needed to corroborate with our findings.

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