scholarly journals Antihelminthic Activity, Phytochemical Profile and Microscopic Features of Ocimum basilicum Collected in DR Congo

2020 ◽  
pp. 42-50
Author(s):  
Carlos N. Kabengele ◽  
Etienne M. Ngoyi ◽  
Giresse N. Kasiama ◽  
Jason T. Kilembe ◽  
Aristote Matondo ◽  
...  

Aim: The main aim of the present study is to validate the bioactivity of O. basilicum, a medicinal plant traditionally used to treat helminthiasis in DRC. Place and Duration of Study: Department of Chemistry, Science Faculty, University of Kinshasa, between June 2018 and March 2019. Methodology: Different parts (stems, leaves and flowers) of O. basilicum and specimens of earthworms of Benhamia rosea were collected and identified at University of Kinshasa. Micrographic examination was carried out using Biolux NV microscope and X-ray fluorescence method for mineral content determination. Radical scavenging activity was performed using the ABTS radical test. Results: Microscopic analysis of O. basilicum powder revealed various histological elements like crystalline fibres, sclerotic fibres, fragments of spiral vessels, pluricellular hairs and glandular hairs. Phytochemical screening in solution and by TLC revealed the presence of polyphenols (flavonoids, anthocyanins, saponins), alkaloids, steroids, coumarins, terpenoids, irridoids and anthraquinones. 20 elements including calcium, sodium, potassium, phosphorus, magnesium, sulphur, chlorine, aluminium, manganese, iron, zinc, copper, strontium, rubidium, bromine, silver, vanadium, neodymium, silicium and lead were determined. The aqueous extract of O. basilicum showed good dose-dependent antihelminthic activity and radical scavenging activity with IC50: 27.04 ± 4.58 µg/mL. Conclusion: This study provides an additional information on the mineral composition and antihelminthic activity of O. basilicum growing in DRC. The antihelminthic activity of this plant could be associated to the presence of flavonoids. To the current knowledge, no study on the histological elements of O. basilicum is available in the literature.

Author(s):  
Amit Subedi ◽  
Mohan Prasad Amatya ◽  
Tirtha Maiya Shrestha ◽  
Shyam Kumar Mishra ◽  
Bharat Mani Pokhrel

The methanol extract of the bark of Machilus odoratissima was subjected to investigate its antioxidant and antibacterial properties. The phytochemical screening demonstrated the presence of different types of compound like terpenoids, tannins, deoxy sugar, saponins and phenolic compounds. The methanol extract of the plant was tested for antioxidant activity using scavenging activity of DPPH(1,1-diphenyl-2-picrylhydrazil) radical method and antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli & Pseudomonas aeruginosa bacteria using cup plate method. The extract exhibited high free radical scavenging activity. IC50 was found to be 3.37μg/ml. Antibacterial activity was observed against S. aureus in dose dependent manner DOI: http://dx.doi.org/10.3126/kuset.v8i1.6045 KUSET 2012; 8(1): 73-80


Author(s):  
Padma S Vankar

Nyctanthes arbor-tristis (Harshingar, Parijat) belongs to the family Oleaceae. The flower has white petals with an orange calyx. Nyctanthes stems have been found to be a rich source of antioxidant. The aqueous extracts of the flower, calyx and petals were evaluated and found to be dose-dependent and showed an increase in DPPH free radical scavenging activity in vitro. The extracts, which showed strong DPPH radical scavenging activity, are in the order Calyx > flower > petals. This signifies that the main antioxidant activity is in the orange colored calyx of the flower. We have attempted an innovative study with the Nyctanthes flower as a part of an exploration for a cheap natural source of antioxidants which can be used at the industrial scale of the food industry.


2021 ◽  
Vol 5 (1) ◽  
pp. 001-008
Author(s):  
Faheem I P ◽  
B. Gopalakrishna ◽  
Mohsina FP ◽  
Sarah Priya

Recently, a substantial body of literature provides strong evidence to support the potentials of Crataeva magna (CM), plant and its extracts in various pathological conditions. Thus, the CM plant extract at various concentrations were prepared and antioxidant activity was evaluated. The fresh leaves and bark of CM were collected and various extracts such as n-Hexane, Chloroform, Ethanol and aqueous extract were prepared. The preliminary phytochemical investigation showed the presence of various phytochemical such as total phenolic, flavonoid compounds and tannins. The ethanolic leaves extract of CM (ELECM) and water leaves extract of CM (WLECM), showed dose dependent increased in free radical scavenging activity in DPPH assay and reducing power assay in present study. Moreover, ethanolic barks extract of CM (EBECM) and water barks extract of CM (WBECM), also showed increased in free radical scavenging activity in DPPH assay and reducing power assay. However, the effects of bark extracts were lower than leaves extracts. In the light of the above consideration, the results of the study revealed that ELECM showed promising antioxidant activity in a dose dependent manner.


2010 ◽  
Vol 2 (3) ◽  
pp. 621 ◽  
Author(s):  
T. Ahsan ◽  
M. A. Rahman ◽  
N. U. Ahmed ◽  
S. B. Uddin

The antioxidant potential of Argyreia argentea methanol stem extract was evaluated by 2,2-diphenyl-1-picrylhydrazyl free radical scavenging method. The extract showed very significant effect as antioxidant in comparison to ascorbic acid, a reference antioxidant. The radical scavenging activity showed by the extract was a dose dependent phenomenon. The extract at the concentration of 400 μg/ml among five different concentrations (10, 50, 100, 200, 400 μg/ml) showed the highest scavenging activity, 94.86%. The stem extract was also evaluated for its cytotoxic properties by measuring significant LC50 value in a Brine-Shrimp lethality bioassay. LC50 value of Argyreia argentea stem extract was found 939.18 μg/ml, at a 95% confidence limit, giving the lower and upper limits 608.98 and 2043.9 μg/ml, respectively. Keywords: Argyreia argentea; DPPH; Ascorbic acid; Scavenging activity; Gallic acid. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i3.4772               J. Sci. Res. 2 (3), 523-530 (2010) 


2017 ◽  
Vol 12 (2) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Victoria Ivanova ◽  
Antoaneta Trendafilova ◽  
Milka Todorova ◽  
Kalina Danova ◽  
Dimitar Dimitrov

The flower heads of Inula britannica L. of Bulgarian origin afforded sesquiterpene lactones (gaillardin, britannin, 11,13-dihydroinuchinenolide B, ivalin, pulchellin C), triterpenoids (3- O-palmitates of 16β-hydroxylupeol, 16β-hydroxy-β-amyrin, and faradiol) and flavonoids (quercetin, luteolin, luteolin-7- O-glucoside). All compounds are known and they were identified by spectral methods. The observed differences in the chemical content of the chloroform and methanol extracts were also reflected in their free radical scavenging activity, evaluated by DPPH and ABTS assays. Intraspecific variability of I. britannica is discussed.


Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
S Ngom ◽  
L Breant ◽  
C Antheaume ◽  
C Minker ◽  
A Leick ◽  
...  

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