scholarly journals Physico-Chemical Properties of Purified Carboxylesterase from the Seeds of Tamarindus indica

Author(s):  
S. Kantharaju ◽  
M. Mylarappa

The present work is focus on physical and chemical properties of purified Carboxylesterase using the Seeds of Tamarindus Indica.The esterases are extracted from the germinating tamarind seeds using 50 mM phosphate buffer, pH 7 and purified. The Km with α-naphthyl acetate, β-naphthyl acetate and α-naphthyl butyrate as the substrates is 28.6 μM, 22.2 μM and 26.7 μM respectively. The Vmax for the same substrates is 7.1 x 10-3 µmole/min, 7.41 x 10-3 µmole/min and 8.00 x 10-3 µmole/min respectively. The enzymes optimally active at pH 7.0 and are stable between pH 5.0 to 8.0. The optimum temperature of esterase activity is 40˚C. The molecular weight of 27.5 kD as determined by SDS-PAGE, both in the presence and absence of β-mercaptothanol and is in close agreement with the molecular weight determined by gel-filtration on Sephadex G-100 (26.9 kD).

1959 ◽  
Vol 26 (2) ◽  
pp. 190-195 ◽  
Author(s):  
R. P. Hansen ◽  
F. B. Shorland ◽  
N. June Cooke

Butterfat has been shown to contain the normal odd-numbered saturated acids n-nonadecanoic acid (C19), n-heneicosanoic acid (C21), and n-tricosanoic acids (C23).The presence of the normal even-numbered acids n-octadecanoic acid (C18), n-docosanoic acid (C22), n-tetracosanoic acid (C24) and n-hexacosanoic acid (C26) is conclusively established.n-Eicosanoic acid (C20) formerly assumed to be present in butterfat has been isolated and identified by its physical and chemical properties.


2019 ◽  
Vol 26 (38) ◽  
pp. 6896-6914 ◽  
Author(s):  
Diana M. Bobrowska ◽  
Piotr Olejnik ◽  
Luis Echegoyen ◽  
Marta E. Plonska-Brzezinska

: This article presents a brief review of the knowledge concerning onion-like carbons (OLCs). These nanostructures are some of the most fascinating carbon forms due to their unusual structure and physico-chemical properties. Generally, OLCs consist of a hollowspherical fullerene core surrounded by concentric graphitic layers with increasing diameter. Nevertheless, they can have different size, shape and type of core, which determine their physicochemical properties. In this article, we review the most important literature reports in this area and briefly describe these nanostructures, their physical and chemical properties and their potential uses with a focus on biomedicine.


1956 ◽  
Vol 29 (2) ◽  
pp. 555-567
Author(s):  
B. A. Dogadkin

Abstract The fundamental reaction of vulcanization is the combination of a vulcanizing agent with rubber. The kinetics of this reaction is expressed by smooth curves. Simultaneously with the combining of the vulcanizing agent, in fact as a result of it, changes take place in a number of physical and chemical properties of rubber—solubility, modulus, tensile strength, and other indexes. Unlike the kinetics of combination of the vulcanizing agent, the changes in these properties are most often represented by curves having a maximum or minimum which characterizes the phenomenon of optimum vulcanization. The extreme form which curves of changes of physical and chemical properties of rubber assume during vulcanization can be explained, in our opinion, by the fact that, during vulcanization, there is a competition between opposing reactions, of which one set are reactions of structure formation (i.e., increase of the molecular weight and the intensity of intermolecular reaction), and the others are destruction reactions. Thus, during vulcanization under factory conditions, at least two reactions take place: (1) the reaction between rubber and sulfur, and (2) the reaction between rubber and molecular oxygen introduced into the vulcanization mix by milling with the ingredients. The amount of oxvgen present here in moles approaches the molar concentration of sulfur.


Author(s):  
Ida Bagus Komang Mahardika ◽  
I Nyoman Rai ◽  
Made Sudiana Mahendra ◽  
Rindang Dwiyani

This study aimed to determine the genetic diversity and fruits quality of the "Jeruk Bali" cultivars grown in Bali. This research was conducted in all regencies and city in Bali, during 2016. Furthermore, several cultivars of “Jeruk Bali” were genetically analyzed based on RAPD markers using 10 primers. Analysis of the quality of fruit is based on physical properties and chemical content. Eighteen cultivars of "Jeruk Bali" obtained have a fruit morphological character with round, short round, and piriform fruit shapes, which are red, pink, cream and white flesh color. RAPD analysis results at 53% similarity level are grouped into 5 groups. The first group was only one cultivar, the second group consisted of 13 cultivars, the third and fourth groups were only one cultivar, while the fifth group consisted of two cultivars. The analysis of the diversity between cultivars based on the combination of physical and chemical properties of the fruit with hierarchy method on similarity level about 85% in a group is obtained by 4 (four) groups. Groupings by combination of physical and chemical properties of the fruit are not synchronized in their entirety with dendograms based on their genetic diversity. This illustrates the physico-chemical properties of “Jeruk Bali” fruit in general is not fully genetical expressed, but also influenced by conditions of environmental growth.


2015 ◽  
Vol 737 ◽  
pp. 65-70
Author(s):  
Dong Bo Yang ◽  
Xing Kai Meng ◽  
Xia Zhang ◽  
Jing Jing Tian

Biodiesel's composition has a significant effect on biodiesel’s main physical and chemical properties. Through the test and analysis of the composition and the physical & chemical properties of 5 types of biodiesel, this paper uses linear regression model and least square method to conduct a research on the correlation between biodiesel's physical and chemical properties such as density, cold filter plugging point (CFPP), cetane number and kinematic viscosity, biodiesel's FAME content and the number of double bonds in its UFAME, and establishes correlation functions for relevant indexes, providing a theoretical basis for rapid test of biodiesel’s physico-chemical performance index.


Author(s):  
Ulzhalgas Nazarbek ◽  
Uilesbek Besterekov ◽  
Perizat Abdurazova ◽  
Saule Nazarbekova ◽  
Yerkebulan Raiymbekov

The article presents the results of physico-chemical research of waste from the phosphorus industry – phosphorus sludge and cottrell dust. The reliability of the experimental results was ensured by the use of generally recognized research methods, as well as reliable physico-chemical and instrumental methods of analysis. The results of the experiment prove the suitability of these types of waste for the production of mineral fertilizers. This, in turn, will make it possible to improve the environmental situation in the southern region of Kazakhstan.


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