scholarly journals Plant Metabolomics: An Overview

2021 ◽  
pp. 56-60
Author(s):  
Ramesh Londonkar ◽  
M. M. Kesaralikar
Keyword(s):  
Analytical Techniques ◽  
Functional Genomic ◽  
Nutritional Values ◽  
Plant Metabolomics ◽  
Plant Remains ◽  
Highly Sensitive ◽  
Metabolomics Study ◽  
Metabolite Pool ◽  
Simultaneous Identification ◽  
Metabolomics Analysis

The term metabolomics was coined by Oliver and his group in 1998. It is a study of biochemical profile and regulation of functions in whole organism by analyzing a metabolite pool present in organism. Researchers believe that more than 400,000 plant species exist worldwide. Total number of metabolites in the plant kingdom are about 2,00,000 to 10,00,000. With the availability of highly sensitive and selective analytical techniques, metabolic changes in plant systems can be followed in a comprehensive way. This technology is useful in assessing gene function and relationships to metabolites. The nutritional values of food and concentration of pharmaceuticals in plants can be improved by using metabolomics study and its functional genomic strategies. Metabolomics analysis is comparatively fast, cheaper and reliable, but simultaneous identification of all metabolites in a crop plant remains a challenge.

Serum metabolomics analysis reveals impaired lipid metabolism in rats after oral exposure to benzo(a)pyrene

2015 ◽  
Vol 11 (3) ◽  
pp. 753-759 ◽  
Author(s):  
Xiaoxue Wang ◽  
Jie Zhang ◽  
Qingyu Huang ◽  
Ambreen Alamdar ◽  
Meiping Tian ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Metabolic Profiling ◽  
Oral Exposure ◽  
Metabolomics Study ◽  
Serum Metabolomics ◽  
Metabolomics Analysis

A metabolomics study was conducted to unveil the metabolic profiling of rats exposed to benzo(a)pyrene, and twelve differentiated metabolites were identified.

scholarly journals ALTERATION OF HISTONES FROM MOUSE EPIDERMAL CELLS AFTER INCUBATION WITH ELASTASE AND HYALURONIDASE

1971 ◽  
Vol 19 (3) ◽  
pp. 182-185 ◽  
Author(s):  
ALVIN SEGAL ◽  
MARGARET SCHROEDER ◽  
BENJAMIN L. VAN DUUREN
Keyword(s):  
Mouse Liver ◽  
Mouse Skin ◽  
Analytical Techniques ◽  
Epidermal Cells ◽  
Ultraviolet Absorption Spectrum ◽  
Tissue Preparation ◽  
Standard Procedures ◽  
Highly Sensitive ◽  
Mammalian Tissues ◽  
Liver Chromatin

Chromatin was isolated from whole mouse skin, mouse epidermal cells and mouse liver by standard procedures used for isolation of chromatin from other mammalian tissues. Chromatin from whole mouse skin or from mouse epidermal cells had not been isolated or characterized earlier. For the preparation of chromatin from mouse epidermal cells, the latter was separated from dermis by incubation for 30 min at 37°C in a solution containing the enzymes elastase and hyaluronidase. The relative proportions of the chromatin components, the T m and the ultraviolet absorption spectrum were all similar to that of chromatin from whole mouse skin which was not treated with enzymes and to other mammalian chromatin preparations. Electrophoresis of the histones from epidermal chromatin in polyacrylamide gels revealed the absence of histones F1, F3 and F2a2 and the appearance of a new band. Histones isolated from chromatin prepared from the whole mouse skin had a gel electrophoresis pattern virtually identical with histones isolated from mouse liver chromatin and to reported histone patterns from other mammalian tissues. The alterations in mouse epidermal histones are similar to reported changes in histones from calf thymus nucleohistone previously subjected to incubation at various temperatures. The enzymatic incubation technique can therefore not be used as a method of isolating unaltered mouse epidermal chromatin. The findings illustrate that very subtle chemical alterations can be induced by usual methods of tissue preparation and that these changes can only be detected by highly sensitive analytical techniques.

Metabolomics analysis revealed significantly higher synovial Phe/Tyr ratio in reactive arthritis and undifferentiated spondyloarthropathy

Rheumatology ◽  
2019 ◽  
Vol 59 (7) ◽  
pp. 1587-1590
Author(s):  
Hafis Muhammed ◽  
Dinesh Kumar ◽  
Durgesh Dubey ◽  
Sandeep Kumar ◽  
Smriti Chaurasia ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Reactive Arthritis ◽  
Nmr Spectra ◽  
Regression Coefficient ◽  
Classification Criteria ◽  
Metabolomics Study ◽  
Metabolomics Analysis

Abstract Objective To compare the synovial phenylalanine/tyrosine (Phe/Tyr) ratio between ReA/uSpA and RA and OA by NMR spectroscopy. Methods Paired SF and serum of 30 patients with ReA/uSpA were collected and analysed using a 1D 1H Carr Purcell Meiboom Gill NMR spectra recorded on 800 MHz NMR spectrometer equipped with a TCI Cryoprobe (at 300 K). Phe and Tyr were quantified. SF from 25 patients with RA fulfilling ACR classification criteria and 21 patients with OA were taken as inflammatory and non-inflammatory controls. Results The synovial Phe/Tyr ratio was significantly higher in ReA/uSpA compared with RA and OA. Synovial Phe/Tyr ratios were comparable in RA and OA patients. Compared with serum, the Phe/Tyr was significantly higher in the SF in ReA/uSpA. The Phe/Tyr ratio was also found to be positively correlated between serum and SF samples, with a regression coefficient (r2) of 0.287. Conclusions This NMR-based metabolomics study demonstrates that the synovial Phe/Tyr ratio is specifically elevated in ReA/uSpA.

scholarly journals Lomatogonium Rotatum for Treatment of Acute Liver Injury in Mice: A Metabolomics Study

Metabolites ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 227 ◽  
Author(s):  
Renhao Chen ◽  
Qi Wang ◽  
Lanjun Zhao ◽  
Shilin Yang ◽  
Zhifeng Li ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Linoleic Acid ◽  
Liver Injury ◽  
Acid Metabolism ◽  
Acute Liver Injury ◽  
Tca Cycle ◽  
Arachidonic Acid Metabolism ◽  
Hepatoprotective Effect ◽  
Metabolomics Study ◽  
Metabolomics Analysis

Lomatogonium rotatum (L.) Fries ex Nym (LR) is used as a traditional Mongolian medicine to treat liver and bile diseases. This study aimed to investigate the hepatoprotective effect of LR on mice with CCl4-induced acute liver injury through conventional assays and metabolomics analysis. This study consisted of male mice (n = 23) in four groups (i.e., control, model, positive control, and LR). The extract of whole plant of LR was used to treat mice in the LR group. Biochemical and histological assays (i.e., serum levels of alanine transaminase (ALT) and aspartate transaminase (AST), and histological changes of liver tissue) were used to evaluate LR efficacy, and metabolomics analysis based on GC-MS and LC-MS was conducted to reveal metabolic changes. The conventional analysis and metabolomic profiles both suggested that LR treatment could protect mice against CCl4-induced acute liver injury. The affected metabolic pathways included linoleic acid metabolism, α-linolenic acid metabolism, arachidonic acid metabolism, CoA biosynthesis, glycerophospholipid metabolism, the TCA cycle, and purine metabolism. This study identified eight metabolites, including phosphopantothenic acid, succinic acid, AMP, choline, glycerol 3-phosphate, linoleic acid, arachidonic acid, and DHA, as potential biomarkers for evaluating hepatoprotective effect of LR. This metabolomics study may shed light on possible mechanisms of hepatoprotective effect of LR.

Development and Validation of Novel GC-FID Method for Simultaneous Quantification of Paracetamol and Caffeine in Formulations

2020 ◽  
Vol 42 (4) ◽  
pp. 581-581
Author(s):  
Imtiaz Hussain Imtiaz Hussain ◽  
Yawar Baig Yawar Baig ◽  
Sara Naveed Sara Naveed ◽  
Muhammad Imran Khan Muhammad Imran Khan ◽  
Hafiz Shoaib Sarwar Hafiz Shoaib Sarwar ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Analytical Techniques ◽  
Hplc Method ◽  
Limit Of Quantification ◽  
Simultaneous Quantification ◽  
Ich Guidelines ◽  
Highly Sensitive ◽  
Sensitivity And Selectivity ◽  
Development And Validation ◽  
Accurate Quantification

The advancements in analytical techniques have enabled the development of highly sensitive and accurate methods for the identification of materials up to trace levels using microliter of sample. A highly sensitive and novel GC-FID method has been developed and reported herein for the simultaneous quantification of paracetamol (PCM) and caffeine (CAF) in samples up to trace levels. The method was validated as per ICH guidelines for its sensitivity, linearity, robustness, inter and intra-day variations, limit of quantification (LOQ), limit of detection (LOD), matrix effect, carryover, precision, and accuracy. The LOD of PCM and CAF were determined to be 100 ppm and 10 ppm whereas, LOQ of PCM and CAF were found to be 300 and 30 ppm respectively. The method was applied to quantify PCM and CAF in marketed tablets and dissolution samples of the tablets, which showed highly precise and accurate quantification of PCM and CAF. Moreover, the GC-FID method was compared with reported HPLC method which suggested the superiority of GC-FID method in performance and sensitivity. In short, the newly developed GC-FID method showed sensitivity and selectivity for PCM and CAF estimation and can be adopted as an efficient method for various applications.

scholarly journals Quantitative assessment of organosulfates in size-segregated rural fine aerosol

2009 ◽  
Vol 9 (1) ◽  
pp. 231-238 ◽  
Author(s):  
H. Lukács ◽  
A. Gelencsér ◽  
A. Hoffer ◽  
G. Kiss ◽  
K. Horváth ◽  
...  
Keyword(s):  
Carbonyl Compounds ◽  
Analytical Techniques ◽  
Organic Aerosol ◽  
Water Soluble ◽  
Heterogeneous Reactions ◽  
Smog Chamber ◽  
Organic Film ◽  
Highly Sensitive ◽  
Fine Aerosol ◽  
Mass Concentrations

Abstract. Organosulfates have recently come into the focus of organic aerosol research as potentially important components of water-soluble secondary organic aerosol (SOA) which now dominate tropospheric fine aerosol. Their presence has been confirmed by the identification of sulfate esters of abundant biogenic carbonyl compounds in both smog chamber and continental aerosol. However, none of the studies have been able to determine the mass contribution of organosulfates to SOA. In this paper, as possibly the very first attempt to quantify organosulfates in ambient aerosol, we inferred the mass concentrations of organosulfates by concurrently determining mass concentrations of total sulfur, sulfate and methanesulfonate in rural fine aerosol using two highly sensitive analytical techniques. Although uncertainties were relatively large, we found that mass concentrations of organosulfates in water-soluble fine aerosol ranged from 0.02 μgS m−3 to 0.09 μgS m−3 yielding a mass contribution of 6–12% to bulk sulfur concentrations (or 6–14% to sulfate concentrations). The inferred size distribution of organosulfates suggested that they possibly form in heterogeneous reactions from semi-volatile carbonyl compounds with subsequent or concurrent condensation of gaseous sulfuric acid producing a refractory organic film on particle surfaces.

scholarly journals Mass spectrometry as a quantitative tool in plant metabolomics

2016 ◽  
Vol 374 (2079) ◽  
pp. 20150370 ◽  
Author(s):  
Tiago F. Jorge ◽  
Ana T. Mata ◽  
Carla António
Keyword(s):  
Mass Spectrometry ◽  
Chemical Species ◽  
Chemical Properties ◽  
Research Field ◽  
Plant Metabolomics ◽  
Wide Range ◽  
Metabolite Pool ◽  
Development And Validation ◽  
Analytical Tools ◽  
Quantitative Tool

Metabolomics is a research field used to acquire comprehensive information on the composition of a metabolite pool to provide a functional screen of the cellular state. Studies of the plant metabolome include the analysis of a wide range of chemical species with very diverse physico-chemical properties, and therefore powerful analytical tools are required for the separation, characterization and quantification of this vast compound diversity present in plant matrices. In this review, challenges in the use of mass spectrometry (MS) as a quantitative tool in plant metabolomics experiments are discussed, and important criteria for the development and validation of MS-based analytical methods provided. This article is part of the themed issue ‘Quantitative mass spectrometry’.

Highly Sensitive Detection of Inorganic Contamination

2009 ◽  
Vol 145-146 ◽  
pp. 101-104 ◽  
Author(s):  
Burkhard Beckhoff ◽  
Andreas Nutsch ◽  
Roswitha Altmann ◽  
G. Borionetti ◽  
C. Pello ◽  
...  
Keyword(s):  
Detection Limits ◽  
Analytical Techniques ◽  
Total Reflection ◽  
Inorganic Contaminants ◽  
Research Activity ◽  
Joint Research ◽  
X Ray ◽  
Low Concentration ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

As the detection of inorganic contaminants is of steadily increasing importance for the improvement of yields in microelectronic applications, the aim of one of the joint research activity within the European Integrated Activity of Excellence and Networking for Nano- and Micro-Electronics Analysis (ANNA, site: www.ANNA-i3.org) is the development and assessment of new methodolo¬gies and metrologies for the detection of low concentration inorganic contaminants in silicon and in novel materials. A main objective consist in the benchmarking of various analytical techniques avail¬able in the laboratories of the participating ANNA partners, including the improvement of the res¬pective detection limits as well as the quantitation reliablity of selected analytical techniques such as total-reflection x-ray fluorescence (TXRF) analysis.

Modern analytical techniques in metabolomics analysis

The Analyst ◽  
2012 ◽  
Vol 137 (2) ◽  
pp. 293-300 ◽  
Author(s):  
Aihua Zhang ◽  
Hui Sun ◽  
Ping Wang ◽  
Ying Han ◽  
Xijun Wang
Keyword(s):  
Analytical Techniques ◽  
Modern Analytical ◽  
Metabolomics Analysis
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