scholarly journals Hematoprotective and Gastroprotective Effects of Cnidoscolus aconitifolius (Linn)-Supplemented Diet in Male Wistar Rats

2020 ◽  
pp. 107-118
Author(s):  
Samuel A. Onasanwo ◽  
Olusegun G. Adebayo ◽  
Omeje U. Nicodemus ◽  
Abayomi M. Ajayi ◽  
A. Wadioni ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Structural Integrity ◽  
Progressive Increase ◽  
Diet Group ◽  
Normal Diet ◽  
Hematological Indices ◽  
Cnidoscolus Aconitifolius ◽  
Male Wistar Rats ◽  
Anti Oxidant ◽  
Plasma Malondialdehyde

Aims: Cnidoscolus aconitifolius (CA) is a plant known to possess very high phenolic compound. The study evaluated hematological indices, anti-oxidative properties and gastroprotective potentials of CA leaf-supplemented diet. Methodology: Male Wistar rats weighing between 80-100 g were divided into 5 groups of n=18. Group 1 animals were fed with normal diet and groups 2-5 were fed with diets supplemented with 1%, 2.5%, 5% and 10% of C. aconitifolius powdered leaves continuously for 4, 8 and 12 weeks. Hematological indices were examined in the whole blood plasma Malondialdehyde (MDA), Glutathione (GSH), Catalase (CAT) were investigated, and changes in stomach architecture were also examined via histology. Results: There was a progressive increase in weight gain at different weeks of feeding with Cnidoscolus aconitifolius-supplemented diet (CAD). Malondialdehyde level decreased (p<0.05) significantly in 1% CAD at 8 weeks of feeding when compared to normal diet group. Glutathione at the 8th and 12th weeks of feeding with 1% CAD and 10% CAD significantly (p<0.05) decreased when compared to the group fed normal diet. Catalase activity increased (p<0.05) significantly in 1%, 2.5% and 5% CAD groups when compared to the group fed normal diet at 4 weeks of feeding. There was increase in haematological indices across all the groups during the weeks of feeding. The microscopic examination of the stomach epithelial layer reveals improvement with no serious damage across all the groups. Conclusion: Cnidoscolus aconitifolius-supplemented diet moderately improves blood anti-oxidant against oxidative stress and the hematological indices with time. The diet also maintains the structural integrity of the stomach wall without causing any damage.

scholarly journals Efek Tepung Kesemek (Diospyros kaki L.var Junggo) terhadap Kadar Trigliserida Serum pada Tikus Wistar Jantan yang Diberi Diet Aterogenik

2017 ◽  
Vol 1 (1) ◽  
pp. 38
Author(s):  
Stefania Widya Setyaningtyas ◽  
Nur Permatasari ◽  
Annasari Mustafa
Keyword(s):  
Triglyceride Level ◽  
Wistar Rats ◽  
Atherogenic Diet ◽  
Diet Group ◽  
Normal Diet ◽  
Control Group ◽  
P Value ◽  
Diospyros Kaki ◽  
Soluble Fiber ◽  
Male Wistar Rats

Background: Moderate hypertriglyceridemia is almost certainly an independent risk factor for cardiovascular disease. Pectin is a kind of soluble fiber that can be used to decrease triglyceride level and it  can be found in fruits such as persimmon. Objective: This research intens to prove the effect of soluble fiber of persimmon flour to decrease triglyceride level. Methods: The search used Post Only Control Group design. Normal diet, atherogenic diet, and atherogenic diet with various amount of dried persimmons were given to 30 subjects, male wistar rats, for 12 weeks. 1.2 g, 2.4 g, and 3.6 g persimmon flour were used as the given amount for every groups. Results: There were significant differences between atherogenic diet group and the other groups of research (p-value<0.001). Compared to normal diet group, atherogenic diet with 1.2 g and 2.4 g persimmon flour resulted unsignificant differences of triglycerides level. But, atherogenic diet with 3,6 g dried persimmon group was different significantly to normal diet groups (p-value=0.036). Both dose of persimmon flour and fat intake affect elevation of tryglyceride level up to 62.5%. Conclusion: The contribution of persimmon flour (Diospyros Kaki L. var Junggo) in inhibitation of the increase of triglyceride serum level on male wistar rats.  The most effective dose is 1.2 g, because the result of the trigliceride level was closest to normal and energy intake was not affected. ABSTRAK Latar belakang : Hipertrigliseridemia kadar sedang hampir pasti merupakan faktor risiko tersendiri untuk penyakit kardiovaskular. Pektin merupakan jenis serat larut air memiliki efek menurunkan trigliserida dan banyak terdapat dalam buah-buahan, salah satunya buah kesemek.Tujuan : Penelitian ini bertujuan menguji efek serat larut air pada tepung kesemek dalam menurunkan kadar trigliserida.Metode : Penelitian dilakukan dengan metode Post Test Only Control Group. Diet normal, diet aterogenik, diet aterogenik + kesemek dengan berbagai jumlah diberikan kepada 30 subyek penelitian, yaitu tikus wistar jantan selama 12 minggu. 1,2 g, 2,4 g, dan 3,6 g tepung kesemek digunakan sebagai intervensi untuk kelompok perlakuan.Hasil : terdapat perbedaan kadar trigliserida yang signifikan antara kelompok diet aterogenik dengan kelompok perlakuan lainnya (p=0,000). Bila dibandingkan dengan kelompok diet normal, perlakuan dengan pemberian tepung kesemek 1,2 g dan 2,4 g menghasilkan kadar trigliserida yang tidak berbeda signifikan, namun jumlah pemberian tepung kesemek 3,6 g berbeda nyata dengan diet normal (p=0,036). Pemberian tepung kesemek dan asupan lemak bersama-sama mempengaruhi pembentukan trigliserida dengan kontribusi sebesar 62,5%.Kesimpulan : Tepung kesemek (Diospyros Kaki L. Var. Junggo) terbukti dapat menghambat peningkatan kadar trigliserida serum pada tikus wistar jantan yang diberi diet aterogenik. Jumlah yang dinilai paling efektif menurunkan trigliserida adalah sebesar 1,2 g, karena pada jumlah tersebut, kadar trigliserida yang dihasilkan paling mendekati normal dan tidak mempengaruhi asupan energi.

scholarly journals Efek Tepung Kesemek (Diospyros kaki L.var Junggo) terhadap Kadar Trigliserida Serum pada Tikus Wistar Jantan yang Diberi Diet Aterogenik

2017 ◽  
Vol 1 (1) ◽  
pp. 38
Author(s):  
Stefania Widya Setyaningtyas ◽  
Nur Permatasari ◽  
Annasari Mustafa
Keyword(s):  
Triglyceride Level ◽  
Wistar Rats ◽  
Atherogenic Diet ◽  
Diet Group ◽  
Normal Diet ◽  
Control Group ◽  
P Value ◽  
Diospyros Kaki ◽  
Soluble Fiber ◽  
Male Wistar Rats

Background: Moderate hypertriglyceridemia is almost certainly an independent risk factor for cardiovascular disease. Pectin is a kind of soluble fiber that can be used to decrease triglyceride level and it  can be found in fruits such as persimmon. Objective: This research intens to prove the effect of soluble fiber of persimmon flour to decrease triglyceride level. Methods: The search used Post Only Control Group design. Normal diet, atherogenic diet, and atherogenic diet with various amount of dried persimmons were given to 30 subjects, male wistar rats, for 12 weeks. 1.2 g, 2.4 g, and 3.6 g persimmon flour were used as the given amount for every groups. Results: There were significant differences between atherogenic diet group and the other groups of research (p-value<0.001). Compared to normal diet group, atherogenic diet with 1.2 g and 2.4 g persimmon flour resulted unsignificant differences of triglycerides level. But, atherogenic diet with 3,6 g dried persimmon group was different significantly to normal diet groups (p-value=0.036). Both dose of persimmon flour and fat intake affect elevation of tryglyceride level up to 62.5%. Conclusion: The contribution of persimmon flour (Diospyros Kaki L. var Junggo) in inhibitation of the increase of triglyceride serum level on male wistar rats.  The most effective dose is 1.2 g, because the result of the trigliceride level was closest to normal and energy intake was not affected. ABSTRAK Latar belakang : Hipertrigliseridemia kadar sedang hampir pasti merupakan faktor risiko tersendiri untuk penyakit kardiovaskular. Pektin merupakan jenis serat larut air memiliki efek menurunkan trigliserida dan banyak terdapat dalam buah-buahan, salah satunya buah kesemek.Tujuan : Penelitian ini bertujuan menguji efek serat larut air pada tepung kesemek dalam menurunkan kadar trigliserida.Metode : Penelitian dilakukan dengan metode Post Test Only Control Group. Diet normal, diet aterogenik, diet aterogenik + kesemek dengan berbagai jumlah diberikan kepada 30 subyek penelitian, yaitu tikus wistar jantan selama 12 minggu. 1,2 g, 2,4 g, dan 3,6 g tepung kesemek digunakan sebagai intervensi untuk kelompok perlakuan.Hasil : terdapat perbedaan kadar trigliserida yang signifikan antara kelompok diet aterogenik dengan kelompok perlakuan lainnya (p=0,000). Bila dibandingkan dengan kelompok diet normal, perlakuan dengan pemberian tepung kesemek 1,2 g dan 2,4 g menghasilkan kadar trigliserida yang tidak berbeda signifikan, namun jumlah pemberian tepung kesemek 3,6 g berbeda nyata dengan diet normal (p=0,036). Pemberian tepung kesemek dan asupan lemak bersama-sama mempengaruhi pembentukan trigliserida dengan kontribusi sebesar 62,5%.Kesimpulan : Tepung kesemek (Diospyros Kaki L. Var. Junggo) terbukti dapat menghambat peningkatan kadar trigliserida serum pada tikus wistar jantan yang diberi diet aterogenik. Jumlah yang dinilai paling efektif menurunkan trigliserida adalah sebesar 1,2 g, karena pada jumlah tersebut, kadar trigliserida yang dihasilkan paling mendekati normal dan tidak mempengaruhi asupan energi.

scholarly journals Effect of Sambiloto (Andrographis paniculata) Leaves against Cholesterol Levels in Wistar Rats with Atherogenic Diet

2021 ◽  
Vol 4 (3) ◽  
Author(s):  
Rizky Dzariyani Laili ◽  
Arie Dwi Alristina ◽  
Rossa Kurnia Ethasari ◽  
Dewinta Hayudanti
Keyword(s):  
Group Treatment ◽  
Wistar Rats ◽  
Atherogenic Diet ◽  
Diet Group ◽  
Normal Diet ◽  
Andrographis Paniculata ◽  
Blood Cholesterol ◽  
Cholesterol Levels ◽  
Significant Difference ◽  
Male Wistar Rats

Introduction : The risk of atherosclerosis increases when elevated blood cholesterol levels. Flavonoids may help inhibit the absorption of fat, which indirectly helps in lower cholesterol levels.Flavonoids are widely available on the Andrographis paniculata leaves (APL).This study aims to examine the effect of flavonoids in APL stewto lower the cholesterol levels. Material and Methods: This research was carried out using five different groups of Wistarrats. Each group consists of five Wistar rats. Group (K-) received normal diet, group (K+) received atherogenic diet and three groups of atherogenic diets rats that pre-treated with three different doses (0.6, 1.2, and 2.4g/day) of APL leaf stew. APL stew is orally gavaged for 60 days. The cholesterol blood serum was analyzed using the CHOD-PAP method. Statistical analysis using One-Way ANOVA and Post-Hoc Tukey. Results : The results showed a significant difference in cholesterol levels between the groups of theatherogenic diet with other treatment groups (p = 0.000).When compared with thenormal diet group, treatment with 1.2 g and 2.4 g of APL produce cholesterol levels not significantly different.APLstew has been shown to inhibit the elevated levels of serum cholesterol in male Wistar rats that fed with the atherogenic diet which contributed 54.8%.While fat intake increased the cholesterol level by 16.3%.Both APL stew and fat dose intake together affect the formation of cholesterol with a contribution of 69.2%. Conclusion : The dose that is considered as the most effective in lowering cholesterol is 2.4 g becauseit producescholesterol level closest to normal.

Tissue Deposition of Zinc from a Zinc Chelate and from Inorganic Zinc in Rats

1994 ◽  
Vol 1994 ◽  
pp. 171-171 ◽  
Author(s):  
Ronan Power ◽  
Kevin Cashman ◽  
Albert Flynn
Keyword(s):  
Amino Acids ◽  
Tissue Distribution ◽  
Wistar Rats ◽  
Gel Filtration ◽  
Normal Diet ◽  
Farm Animals ◽  
Trace Minerals ◽  
Inorganic Salts ◽  
Male Wistar Rats ◽  
Differential Tissue

Some reports have suggested differential tissue deposition of dietary trace minerals such as Zinc (Zn) when supplied to farm animals either chelated to amino acids or as inorganic salts. To test this hypothesis, an experiment was conducted to determine the ultimate tissue distribution of Zinc in rats fed either a radioactively-labeled 65Zn-chelate or 65ZnSO4. The 65Zn-chelate was prepared by heating a solution of 65ZnSO4 and an equimolar mixture of glycine and methionine for 5 minutes at 90°C. The resulting chelate was then separated from unincorporated 65ZnSO4 by gel filtration chromatography. Ten 25-d old male wistar rats (mean weight 34.5 g) were randomized by weight into two groups (n = 5/group), fasted for 18 hours and given 0.4 ml (8 μg Zn, 1 μCi65Zn) of one or other labelled solution by gavage. Four hours later, animals were returned to their normal diet for the duration of the experiment. The 65Zn activity of the animals was determined two hours after administration and daily thereafter for 7 days.

Cnidoscolus aconitifolius-supplemented diet enhanced neurocognition, endogenous antioxidants and cholinergic system and maintains hippocampal neuronal integrity in male Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Olusegun G. Adebayo ◽  
Samuel A. Onasanwo ◽  
Abayomi M. Ajayi ◽  
Wadioni Aduema ◽  
Oyetola T. Oyebanjo ◽  
...  
Keyword(s):  
Cholinergic System ◽  
Water Maze ◽  
Wistar Rats ◽  
Memory Performance ◽  
Novel Object Recognition ◽  
Object Recognition Test ◽  
Novel Object ◽  
Cnidoscolus Aconitifolius ◽  
Novel Object Recognition Test ◽  
Male Wistar Rats

Abstract Objectives Cnidoscolus aconitifolius have been investigated to have abundant phytochemicals. However, study on the effect of Cnidoscolus aconitifolius on neurobehavioral performance when supplemented with diet is lacking. The study is aimed at investigating the memory-enhancing effect of Cnidoscolus aconitifolius-supplemented diet (CAD) using Morris water maze and Novel object recognition test. Methods Ninety male Wistar rats (80–100 g) were fed with CAD (1, 2.5, 5 and 10%) continuously for a period of 4, 8 and 12 weeks respectively. Six animals per group were used for assessment of memory performance (Morris water maze [MWM] and Novel object recognition test [NORT]); afterwards the brain tissues were harvested for malondialdehyde (MDA), glutathione (GSH) and catalase (CAT) estimation. Acetylcholinesterase (AChE) concentration was also determined. Hippocampal architectural change in the neuron was examined using hematoxylin and eosin (H&E) and cresyl fast violet (Nissl) stain. Results Higher percentage of CAD significantly (p<0.05) improve memory performance with time-dependent effects in rats fed with CAD on MMW and NORT. MDA significantly (p<0.05) reduce in 1 and 2.5% CAD groups at 4th weeks and in 2.5 and 5% CAD groups at 8th weeks while GSH concentration significantly (p<0.05) increase at 12th weeks in 2.5 and 10% CAD groups. However, CAT concentration significantly (p<0.05) increase in 2.5, and 5%, CAD groups, 1, 5, and 10% CAD groups and in 5, and 10% CAD groups at 4th, 8th and 12th weeks. AChE significantly (p<0.05) reduce at 4th and 12th weeks. Histological assessment reveals no neuronal and pyramidal degeneration (chromatolysis) at the hippocampal Cornu Ammonis 3 (CA3) region. Conclusions The results suggest that CAD boost memory performance in rats through positive modulation of oxidative stress, cholinergic system and degeneration of hippocampal neurons.

scholarly journals Effect of Kefir, Yacon (Smallanthus sonchifolius) and the Association Between Yacon and Kefir on Intestinal Physiology in Rats With Colon Cancer

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 309-309
Author(s):  
Neuza Costa ◽  
Keila Zanardi ◽  
Caroline Woelffel ◽  
Andre Costa ◽  
Mirelle Viana ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Intestinal Permeability ◽  
Wistar Rats ◽  
Short Chain Fatty Acids ◽  
Diet Group ◽  
Standard Diet ◽  
Smallanthus Sonchifolius ◽  
Intestinal Physiology ◽  
Significant Difference ◽  
Male Wistar Rats

Abstract Objectives To evaluate the effect of consumption of yacon flour, kefir and the association between them on colon cancer induced by 1,2-dimethylhydrazine (DMH) in male Wistar rats, SCFA production, fecal pH and intestinal permeability. Methods The study was conducted with 60 adult Wistar rats divided into 5 groups. For 5 weeks, groups T, Y, K and YK received 1,2-dimethylhydrazine (DMH) to induce colon cancer. After 5 weeks of DMH administration, animals in groups C and T received the standard diet, group Y received a diet with yacon flour with 5% FOS, group K received 1mL /day of kefir and the YK group received a diet with yacon and kefir, for 15 weeks. After euthanasia, intestinal lesions, intraluminal pH, short-chain fatty acids (SCFA) and intestinal permeability were analyzed. Results An increase in macroscopic lesions was observed in groups K (58%) and YK (42%) and a reduction of 5% in group Y, compared to group T. In addition, an increase in neoplastic changes was observed in all groups compared to group T: Y (33%), K (67%) and YK (78%). There was no significant difference in the concentrations of acetate and proprionate, pH, lactulose and mannitol between groups, and butyrate was not found in the samples. Conclusions The consumption of yacon flour, kefir and their association did not influence intestinal physiology and promoted the worsening of the development of colon carcinogenesis in rats. Funding Sources FAPES - Fundação de Amparo à Pesquisa e Inovação do Espírito Santo CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico

scholarly journals The Effect of Soy Milk on Mounting Latency, Mounting Frequency, and Reproductive Development in Male Wistar Rats (Rattus Norvegicus)

2021 ◽  
Vol 9 (B) ◽  
pp. 670-678
Author(s):  
Nurdiana Nurdiana ◽  
Pradnyawati Chania ◽  
Rifzi Nurvitasari ◽  
Azmiatun Nisa ◽  
Styan Wahyu Diana ◽  
...  
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Reproductive Development ◽  
Normal Diet ◽  
Male Rats ◽  
Male Rat ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Soy Milk ◽  
Male Wistar Rats

AIM: This research aims to examine the effects of soy milk on mounting latency (ML), mounting frequency (MF), estrogen levels, androgen-binding protein (ABP) expression, and spermatogenesis in male rats (Rattus norvegicus). METHODS: Twenty-four male wistar rats (Rattus norvegicus) aged 4 weeks were divided into four groups. Control group (given a normal diet), P1; P2; P3 (given the normal diet and soy milk powder at doses of 7.1; 14.2; 21.3 g/KgBW/day, respectively) for 6 weeks. Observation of ML and MF were performed at 9 weeks 5 days of age, and rat surgery was performed at 10 weeks of age. Analysis of estrogen hormone levels was conducted by enzyme-linked immunosorbent assay (ELISA), ABP staining was using immunohistochemistry method, testicular spermatogenesis was observed using histopathological methods, and observation of spermatozoa was performed under the microscope.  RESULTS: The results showed no significant reduction of ML and MF, estrogen levels, and ABP expression (p ≤ 0.256; 0.865; 0.959, respectively) in male rat, but there was a significant decrease in the number, morphology, motility of spermatozoa, and testicular histophatology, (p ≤ 0.000, 0.003, 0.008, 0.000, respectively). CONCLUSION: The administrassion of soy milk in various doses (7.1;14.2;21.3 g/KgBW/day) in male Wistar rats (Rattus norvegicus) had showed significantly difference on histopathological evaluation using Johnson’s scoring system, sperm quantity and quality, while on mounting latency and frequency, estrogen levels, and ABP expressions did not show significantly difference between groups. That describe of isoflavone in soy milk can affect several aspects related to male endocrine and reproductive development.

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