Description of a Harmless Method for Determining Trenbolone Acetate Together with 17B-Trenbolone in Beef

2021 ◽  
pp. 74-80
Author(s):  
Naoto Furusawa
Keyword(s):  
Trenbolone Acetate

Effects of Dried Distillers’ Grains Cube Supplementation for Steers Grazing Introduced Pastures on Animal Performance and Forage Production

2021 ◽  
Vol 99 (Supplement_2) ◽  
pp. 12-13
Author(s):  
Jordan Adams ◽  
Rodney Farris ◽  
Scott Clawson ◽  
Earl Ward ◽  
Paul Beck
Keyword(s):  
Festuca Arundinacea ◽  
Statistical Power ◽  
Cynodon Dactylon ◽  
Animal Health ◽  
Late Summer ◽  
Early Summer ◽  
Distillers Grains ◽  
Forage Production ◽  
Trenbolone Acetate ◽  
Dried Distillers Grains

Abstract We evaluated the effects of supplementing dried distillers’ grains cubes (DDGS) and re-implantation of steers (n = 149; BW = 238 ± 13.8 kg) grazing tall fescue (Festuca arundinacea)/bermudagrass (Cynodon dactylon) pastures (n = 9 pastures, 7.2 ± 2.90 ha) from 14 April to 17 September 2020 (n = 155 d) in a split-plot design on steer performance and forage production. Main plot supplemental treatments (n = 3 pastures/treatment) included 1) Fertilized Control (FC), no supplementation on fertilized pastures (112 kg N/ha); 2) Fertilized Supplement (FS), supplemental DDGS fed at 2.9 kg 3-d/wk on fertilized pastures; and 3) Supplement (S), supplemented DDGS at 0.75% BW/d on unfertilized pastures prorated for 5-d/wk feeding. Steers were previously implanted during receiving with 40 mg trenbolone acetate and 8 mg estradiol (REV-G; Revalor G, Merck Animal Health). On July 7, steers in each pasture were randomly assigned to one of three re-implant treatments: 1) no re-implant; 2) REV-G; or 3) 200 mg progesterone and 20 mg estradiol (Synovex S, Zoetis Animal Health). Steers in FS and S gained more (P < 0.01) than FC throughout the trial and final BW was greater (P < 0.01) for FS and S compared with FC. Unexpectedly, re-implanting had no effect on ADG (P = 0.57) or BW (P = 0.34), but statistical power may be lacking. Supplemental efficiency was greater in the late summer for FS (P = 0.05) compared to S. Fertilizing pastures in FS and FC did not affect biomass (P = 0.39), however, CP was increased (P = 0.01) and acid and neutral detergent fibers tended to decrease (P = 0.06) relative to S in the early summer (April, May, June, and July), but did not differ in late summer (August and September). Based upon our analysis, DDGS is a suitable supplement and can replace N fertilizer for steers grazing introduced pastures.

scholarly journals 179 Using a 100% Dried Distillers Grains Cube as a Supplement for Steers Grazing Mixed Grass Prairie in Oklahoma

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 46-46
Author(s):  
Zane N Grigsby ◽  
Paul A Beck ◽  
Stacey A Gunter
Keyword(s):  
Animal Health ◽  
Late Summer ◽  
Estradiol Benzoate ◽  
Positive Control ◽  
Negative Control ◽  
Early Summer ◽  
Trenbolone Acetate ◽  
Mixed Grass Prairie ◽  
Mixed Grass ◽  
Main Plot

Abstract This research was conducted to determine effects of supplementation and implanting on BW gain by steers grazing mixed grass prairie (n = 12 pastures, 19.9 ± 0.7 ha) in northwest Oklahoma. Three main plot treatments were: 1) Negative Control (NC), no supplementation, 2) Positive Control (PC), supplemented with DDGS cubes, 1.8 kg/steer on alternate days in late summer, 3) High Supplement (HS), 1/3 increase in stocking rate with 0.75% BW supplemental DDGS cubes all season. Steers (n = 125, BW = 223.1 ± 23.2 kg) were stocked at 2.2 ha/steer for PC and NC, 1.3 ha/steer for HS. Grazing was from May 17 – September 27 (132 d). All steers were implanted with 200 mg progesterone and 20 mg estradiol benzoate (SYN, Synonvex S, Zoetis Animal Health) on May 17. On July 18 three reimplant treatments were applied: 1) no reimplant; 2) SYN; or 3) 40 mg trenbolone acetate and 8 mg estradiol (Revalor G, Merck Animal Health). Data were analyzed using the PROC MIXED in SAS as a split-plot experimental design. In early summer HS had 0.26 kg greater (P < 0.01) ADG than NC and PC. Late summer gains of PC were 0.33 kg/d more (P ≤ 0.01) than NC; and HS gained 0.49 and 0.16 kg/day more (P ≤ 0.04) than NC and PC, respectively. Gain per hectare for PC (46 kg/ha) were greater (P < 0.01) than NC (35 kg/ha) and more than doubled (P < 0.01) with HS (89 kg/ha). Reimplanting had no effect on ADG (P ≥ 0.28). Late season supplementation with PC resulted in supplemental efficiency of 2.7 kg supplement/kg added gain compared with NC. Increased stocking rates with season long supplementation in HS resulted in supplemental efficiency of 3.8 kg supplement/kg added gain per hectare. Based on these data, a 100% DDGS cube is an effective supplement option to increase BW gain during the late summer or increase carrying capacity and gain during the summer grazing period in northwestern Oklahoma.

Roles of IGF-I and the estrogen, androgen and IGF-I receptors in estradiol-17β- and trenbolone acetate-stimulated proliferation of cultured bovine satellite cells

2008 ◽  
Vol 35 (1) ◽  
pp. 88-97 ◽  
Author(s):  
E. Kamanga-Sollo ◽  
M.E. White ◽  
M.R. Hathaway ◽  
K.Y. Chung ◽  
B.J. Johnson ◽  
...  
Keyword(s):  
Satellite Cells ◽  
Trenbolone Acetate ◽  
Igf I ◽  
Estradiol 17Β

Effects of trenbolone acetate and testosterone on growth and on plasma concentrations of corticosterone and ACTH in rats

1990 ◽  
Vol 126 (3) ◽  
pp. 461-466 ◽  
Author(s):  
M. N. Sillence ◽  
R. G. Rodway
Keyword(s):  
Weight Gain ◽  
Growth Response ◽  
Plasma Concentrations ◽  
Female Rats ◽  
Male Rats ◽  
Adrenal Weight ◽  
Trenbolone Acetate ◽  
Male And Female Rats ◽  
Age Related ◽  
Old Rats

ABSTRACT The effects of trenbolone acetate (TBA) on growth and on plasma concentrations of corticosterone were examined in male and female rats. At 5 weeks of age, rats were injected with TBA (0·8 mg/kg) dissolved in peanut oil, or with oil alone, daily for 10 days. In female rats, TBA caused an increase in weight gain (20–38%), a reduction in adrenal weight (19%) and a reduction in plasma concentrations of corticosterone (55%). In contrast, TBA-treated male rats showed no significant increase in weight gain, no significant change in adrenal weight and no reduction in plasma concentrations of corticosterone. The mechanism by which adrenal activity was suppressed in TBA-treated female rats was examined and the response compared with that to testosterone. Female rats (8 weeks old) were injected daily either with oil vehicle, TBA (0·8 mg/kg) or testosterone propionate (0·8 mg/kg). Testosterone increased weight gain (24%), but the growth response to TBA treatment was significantly greater (97%). A reduction in plasma concentrations of corticosterone (45%) was again observed in response to TBA. However, testosterone increased plasma concentrations of corticosterone (52%) above those of control values. Neither androgen affected plasma concentrations of ACTH. Finally, the effects of TBA were examined in 6-week-old female rats, to characterize further the apparent age-related increase in responsiveness. The growth response of 6-week-old rats (60–74%) was intermediate between that seen in 5- and 8-week-old animals. It is concluded that part of the anabolic activity of TBA may be related to a reduction in circulating concentrations of corticosterone. The effect of TBA on corticosterone concentrations differs from that of the natural androgen, testosterone, and does not appear to be mediated by a reduction in plasma concentrations of ACTH. Journal of Endocrinology (1990) 126, 461–466

Effect of naturally occurring and synthetic androgens on growth, body composition and muscle glucocorticoid receptors in wether lambs

1994 ◽  
Vol 58 (3) ◽  
pp. 357-364 ◽  
Author(s):  
H. Gallbraith ◽  
A. D. Berry
Keyword(s):  
Skeletal Muscle ◽  
Body Weight ◽  
Crude Protein ◽  
Binding Capacity ◽  
Live Weight ◽  
Backfat Thickness ◽  
Trenbolone Acetate ◽  
Naturally Occurring ◽  
Main Effects ◽  
Nandrolone Phenylpropionate

AbstractTwenty-five Border Leicester ♂ × Blackface ♀ wether lambs aged about 4 months and weighing on average 28·5 kg were allocated to be treated with the naturally occurring steroid testosterone or trenbolone acetate or nandrolone phenylpropionate which are steroids synthetically produced. Treatment groups were as follows: untreated controls (C); 50 mg testosterone (T); 50 mg trenbolone acetate (TA); 50 mg testosterone + 50 mg trenbolone acetate (TTA) or 50 mg nandrolone phenylpropionate (N). Implants were given at 100 and again at 63 days before slaughter. The lambs were offered to appetite a good quality diet containing, per kg dry matter, an estimated 11·0 MJ metabolizable energy and 185 g crude protein. Comparisons were made for the main effects of T and TA and also interactions between T and TA. Effects due to N were assessed statistically against untreated controls. Treatment with T, on average, increased live-weight gain (LWG), empty body weight (EBW) and reduced backfat thickness and the weight (g/kg EBW) of perirenal and retroperitoneal fat. Main effects due to TA were increases in killing-out ratio and depth of the gigot joint and reductions in backfat thickness. Treatment with N increased the empty body weight and (g/kg) carcass ash. Non-significant (P > 0·05) trends were suggested for increases in carcass crude protein due to T and TA treatments. T and TA but not N treatments exhibited marked androgenic activity in increasing the weight (mg/kg EBW) of the accessary vesicular gland. TA and N, but not T, reduced the weight (g/kg EBW) of the thymus gland.The maximum binding capacity of post-morte m skeletal muscle (m. gluteus) for (3H)-dexamethasone was reduced by TA but increased by T and N. These results suggest differences in the binding capacity of corticosteroid receptors which may be related to differences in the effects of T and TA on protein metabolism in skeletal muscle.

Efficacy of Trenbolone Acetate in Sex Inversion of the Blue Tilapia Oreochromis aureus

1996 ◽  
Vol 27 (4) ◽  
pp. 483-486 ◽  
Author(s):  
John I. Galvez ◽  
John R. Morrison ◽  
Ronald P Phelps
Keyword(s):  
Oreochromis Aureus ◽  
Trenbolone Acetate ◽  
Sex Inversion ◽  
Blue Tilapia

scholarly journals Effects of coated and noncoated steroidal implants on growth performance, carcass characteristics, and serum estradiol-17β concentrations of finishing Holstein steers

2020 ◽  
Vol 4 (4) ◽  
Author(s):  
Pedro H V Carvalho ◽  
Mariana F Westphalen ◽  
Jonathan A Campbell ◽  
Tara L Felix
Keyword(s):  
Growth Performance ◽  
Animal Health ◽  
Average Daily Gain ◽  
Carcass Characteristics ◽  
Feed Ratio ◽  
Serum Estradiol ◽  
Trenbolone Acetate ◽  
Coated Implant ◽  
Daily Gain ◽  
Estradiol 17Β

Abstract The objectives of the study were to determine the effect of coated or noncoated hormone implants on growth performance, carcass characteristics, and serum estradiol-17β (E2) concentrations of Holstein steers fed a grain-based diet for 112 d. Seventy-nine Holstein steers [average initial body weight (BW) = 452 ± 5.5 kg] were stratified by BW and allotted to one of two treatments: 1) Holstein steers implanted with a coated implant containing 200 mg of trenbolone acetate (TBA) and 40 mg E2 (Revalor-XS (Merck Animal Health; Summit, NJ)] on day 0 (XS) or 2) Holstein steers implanted two times (days 0 and 56) with a noncoated implant containing 80 mg of TBA and 16 mg of E2 [(2IS) Revalor-IS (Merck Animal Health)]. Data were analyzed using the MIXED procedure of SAS (SAS Inst. Inc., Cary, NC). There was no effect (P ≥ 0.71) of implant strategy on initial, middle, and final BW. No effect (P ≥ 0.12) of implant strategy was observed on average daily gain, dry matter intake, or gain-to-feed ratio. There were no effects (P ≥ 0.11) of implant strategy on carcass characteristics. There was an implant × day interaction (P < 0.01) for the circulation of serum E2 concentrations. Serum E2 concentration increased similarly 14 d after Holstein steers were implanted, regardless of implant strategy. At 28 d, after steers were implanted, steers in the XS group had less serum E2 concentration than Holstein steers in the 2IS group. However, at 56 d after the first implantation, both groups, once again, had similar serum E2 concentrations and E2 concentrations were less on day 56 than day 28 for both strategies. Holstein steers implanted with 2IS had greater serum E2 concentration on day 70 and E2 concentrations remained greater than serum E2 of Holstein steers implanted XS for the duration of the trial (day 112). In summary, there was no effect of coated or two doses of noncoated implant on growth performance or carcass characteristics of Holstein steers.

scholarly journals PSIII-8 Impact of SYNOVEX® ONE GRASS implantation during suckling on pre-weaning calf growth performance

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 239-239
Author(s):  
Shane Newton ◽  
Payton Dahmer ◽  
Larry Corah ◽  
Keith Harmoney ◽  
John Jaeger ◽  
...  
Keyword(s):  
Growth Performance ◽  
Random Effect ◽  
Cost Effective ◽  
Recent Decade ◽  
Estradiol Benzoate ◽  
Beef Steers ◽  
Weaning Weight ◽  
Trenbolone Acetate ◽  
Calf Growth ◽  
Randomized Design

Abstract Historic data supports the use of pre-weaning implants to maximize beef calf weaning weight, but their use has declined over the recent decade. A total of 96 beef steers (initially 85.2 kg BW and > 45 d of age) were used in a 168-d study to evaluate the effects of Synovex C® implantation during suckling on pre-weaning calf growth performance. At initial processing, calves were individually weighed and allotted in a completely randomized design to one of two treatments: 1) not implanted (control) or 2) implanted with 150 mg of trenbolone acetate and 21 mg of estradiol benzoate (SYNOVEX® ONE GRASS, Zoetis, Kalamazoo, MI). All calves were weighed on d 0 (implantation), 83, 126 and 168 (weaning), with ADG calculated for each period. Data were analyzed using the GLIMMIX procedure of SAS (v9.4, Cary, NC). There were 48 replicates per treatment, with grazing location as a random effect. While there were no detected differences in BW during the initial phases (P > 0.10), implanted calves tended to have greater (P = 0.100) greater weaning weight than non-implanted calves (279 vs. 265 kg, respectively). Calves implanted with SYNOVEX® ONE GRASS had 0.8, 0.12, and 0.16 kg/d greater (P < 0.05) ADG than those that were not implanted in all three phases. This resulted in an average of 0.10 kg/d greater (P = 0.002) ADG in implanted calves overall compared to the control. In conclusion, this research showed significant increases in suckling calf ADG in implanted calves compared to their non-implanted counterparts, which led to a tendency for 14-kg greater weaning weights. Additional research is warranted to consider the post-weaning growth and impact of re-implanting, but this data reinforces previous literature that suckling calf implantation continues to be cost effective for cow-calf producers.

PSX-B-5 Effect of replacement of dry-rolled corn with un-processed rye on growth performance, efficiency of dietary net energy utilization, and carcass traits of finishing heifers

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 458-459
Author(s):  
Keith M Buckhaus ◽  
Warren C Rusche ◽  
Zachary K Smith
Keyword(s):  
Body Weight ◽  
Growth Performance ◽  
Block Design ◽  
Average Daily Gain ◽  
Estradiol Benzoate ◽  
Energy Utilization ◽  
Net Energy ◽  
Final Body Weight ◽  
Trenbolone Acetate ◽  
Finishing Diets

Abstract Continental × British beef heifers were used in a randomized complete block design experiment to evaluate the effects of replacing dry-rolled corn with unprocessed rye on growth performance, efficiency of dietary net energy (NE) utilization, and carcass trait responses in finishing heifers. Heifers (n = 56; 433 ± 34.0 kg) were transported 241 km from a regional sale barn to the Ruminant Nutrition Center in Brookings, SD. Heifers were blocked by weight grouping and then allotted to pens (n = 7 heifers/pen and 4 pens/treatment). Treatments included a finishing diet that contained 60% grain (DM basis) as dry-rolled corn (DRC) or unprocessed rye grain (RYE). On d 14, heifers were consuming the final diet and were implanted with 200 mg of trenbolone acetate and 28 mg of estradiol benzoate (Synovex-Plus, Zoetis, Parsippany, NJ). RYE heifers had decreased (P ≤ 0.01) final body weight, average daily gain, and gain efficiency; but tended (P = 0.08) to have a greater dry matter intake compared to DRC. RYE had decreased (P ≤ 0.01) observed dietary NE and decreased (P ≤ 0.01) observed-to-expected dietary NE ratio for maintenance and gain compared to DRC. Dressing percentage, 12th rib fat thickness, ribeye area, and the distribution of USDA yield and quality grades were not altered (P ≥ 0.12) by diet. Hot carcass weight, yield grade, estimated empty body fat (EBF), and body weight at 28% EBF decreased (P ≤ 0.02) and retail yield increased (P= 0.01) in RYE compared to DRC. These data indicate that unprocessed rye is a palatable feed ingredient for inclusion in finishing diets for beef cattle and that rye inclusion only minimally influences carcass quality. The feeding value of unprocessed rye is considerably less (21.4%) than that of dry-rolled corn using current standards and approximately 91% of the NE value of processed rye.

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