179 Using a 100% Dried Distillers Grains Cube as a Supplement for Steers Grazing Mixed Grass Prairie in Oklahoma

Abstract This research was conducted to determine effects of supplementation and implanting on BW gain by steers grazing mixed grass prairie (n = 12 pastures, 19.9 ± 0.7 ha) in northwest Oklahoma. Three main plot treatments were: 1) Negative Control (NC), no supplementation, 2) Positive Control (PC), supplemented with DDGS cubes, 1.8 kg/steer on alternate days in late summer, 3) High Supplement (HS), 1/3 increase in stocking rate with 0.75% BW supplemental DDGS cubes all season. Steers (n = 125, BW = 223.1 ± 23.2 kg) were stocked at 2.2 ha/steer for PC and NC, 1.3 ha/steer for HS. Grazing was from May 17 – September 27 (132 d). All steers were implanted with 200 mg progesterone and 20 mg estradiol benzoate (SYN, Synonvex S, Zoetis Animal Health) on May 17. On July 18 three reimplant treatments were applied: 1) no reimplant; 2) SYN; or 3) 40 mg trenbolone acetate and 8 mg estradiol (Revalor G, Merck Animal Health). Data were analyzed using the PROC MIXED in SAS as a split-plot experimental design. In early summer HS had 0.26 kg greater (P < 0.01) ADG than NC and PC. Late summer gains of PC were 0.33 kg/d more (P ≤ 0.01) than NC; and HS gained 0.49 and 0.16 kg/day more (P ≤ 0.04) than NC and PC, respectively. Gain per hectare for PC (46 kg/ha) were greater (P < 0.01) than NC (35 kg/ha) and more than doubled (P < 0.01) with HS (89 kg/ha). Reimplanting had no effect on ADG (P ≥ 0.28). Late season supplementation with PC resulted in supplemental efficiency of 2.7 kg supplement/kg added gain compared with NC. Increased stocking rates with season long supplementation in HS resulted in supplemental efficiency of 3.8 kg supplement/kg added gain per hectare. Based on these data, a 100% DDGS cube is an effective supplement option to increase BW gain during the late summer or increase carrying capacity and gain during the summer grazing period in northwestern Oklahoma.

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Effects of Dried Distillers’ Grains Cube Supplementation for Steers Grazing Introduced Pastures on Animal Performance and Forage Production

Journal of Animal Science ◽

10.1093/jas/skab096.020 ◽

2021 ◽

Vol 99 (Supplement_2) ◽

pp. 12-13

Author(s):

Jordan Adams ◽

Rodney Farris ◽

Scott Clawson ◽

Earl Ward ◽

Paul Beck

Keyword(s):

Festuca Arundinacea ◽

Statistical Power ◽

Cynodon Dactylon ◽

Animal Health ◽

Late Summer ◽

Early Summer ◽

Distillers Grains ◽

Forage Production ◽

Trenbolone Acetate ◽

Dried Distillers Grains

Abstract We evaluated the effects of supplementing dried distillers’ grains cubes (DDGS) and re-implantation of steers (n = 149; BW = 238 ± 13.8 kg) grazing tall fescue (Festuca arundinacea)/bermudagrass (Cynodon dactylon) pastures (n = 9 pastures, 7.2 ± 2.90 ha) from 14 April to 17 September 2020 (n = 155 d) in a split-plot design on steer performance and forage production. Main plot supplemental treatments (n = 3 pastures/treatment) included 1) Fertilized Control (FC), no supplementation on fertilized pastures (112 kg N/ha); 2) Fertilized Supplement (FS), supplemental DDGS fed at 2.9 kg 3-d/wk on fertilized pastures; and 3) Supplement (S), supplemented DDGS at 0.75% BW/d on unfertilized pastures prorated for 5-d/wk feeding. Steers were previously implanted during receiving with 40 mg trenbolone acetate and 8 mg estradiol (REV-G; Revalor G, Merck Animal Health). On July 7, steers in each pasture were randomly assigned to one of three re-implant treatments: 1) no re-implant; 2) REV-G; or 3) 200 mg progesterone and 20 mg estradiol (Synovex S, Zoetis Animal Health). Steers in FS and S gained more (P < 0.01) than FC throughout the trial and final BW was greater (P < 0.01) for FS and S compared with FC. Unexpectedly, re-implanting had no effect on ADG (P = 0.57) or BW (P = 0.34), but statistical power may be lacking. Supplemental efficiency was greater in the late summer for FS (P = 0.05) compared to S. Fertilizing pastures in FS and FC did not affect biomass (P = 0.39), however, CP was increased (P = 0.01) and acid and neutral detergent fibers tended to decrease (P = 0.06) relative to S in the early summer (April, May, June, and July), but did not differ in late summer (August and September). Based upon our analysis, DDGS is a suitable supplement and can replace N fertilizer for steers grazing introduced pastures.

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29 Demonstrating the Effects of Deworming and Implanting Weaned Calves for Retained Ownership on Pasture in Eastern Oklahoma

Journal of Animal Science ◽

10.1093/jas/skz397.013 ◽

2020 ◽

Vol 98 (Supplement_2) ◽

pp. 6-6

Author(s):

Paul A Beck ◽

Earl Ward ◽

Scott Clawson ◽

Brian Pugh ◽

Rodney Farris

Keyword(s):

Performance Enhancement ◽

Animal Health ◽

Economic Benefits ◽

Early Summer ◽

Research Station ◽

Mixed Grass ◽

Main Plot ◽

Growth Promoting ◽

Retained Ownership ◽

Implant Treatment

Abstract The majority of cow-calf producers in Oklahoma sell their calves at weaning. Preconditioning and retained ownership through the stocker phase provides economic benefits to these operations. The objectives of this demonstration were to illustrate the benefits of growth promoting technologies for calves post-weaning. Calves (n = 39 heifers and 24 steers; BW = 229 ± 23.9 kg) from the OSU Eastern Research Station cowherd were weaned on day – 20 (April 15) and preconditioned. On day 0 (May 6), calves (n = 20 heifers and 12 steers) were treated for internal parasites (Dectomax, Zoetis Animal Health) and evenly allocated to 2 Bermudagrass/tall fescue pastures (9.5 ± 1.42 ha) for 143 days. The remaining calves (n = 19 heifers and 12 steers) were not treated for parasites and allocated to 2 pastures (9.1 ± 1.42 ha). In each pasture, ½ of the calves of each sex were implanted (Synovex-S or Synovex-H, Zoetis). Calves dewormed on day 0 and were dewormed again on day 70 (July 16). Data were analyzed as a split plot experimental design with dewormer treatment as the main plot and calf sex and implant treatment were the split plots. There were no interactions among calf sex, deworming, and implants (P ≥ 0.42). Steers were heavier (P < 0.01) than heifers throughout the summer, but did not have an advantage in gain performance (P ≥ 0.22). Deworming did not impact (P = 0.44) overall growth performance of calves, but did numerically (P = 0.18) increase ADG from day 71 to 143 by 0.15 kg/d. Growth promoting implants increased (P = 0.03) daily gains by 0.09 kg/day in the early summer and over the entire summer by 0.07 kg/day (P < 0.01). The combination of deworming and implants increased (P = 0.01) season long ADG by 0.11 kg/day over unimplanted controls. This demonstration was used to illustrate the potential that growth promoting implants and deworming provides for economically beneficial performance enhancement for retained stocker calves on mixed grass pasture.

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Severe Outbreak of Bacterial Blight Caused by Xanthomonas arboricola pv. corylina on Hazelnut cv. Tonda di Giffoni in Central Italy

Plant Disease ◽

10.1094/pdis-04-12-0375-pdn ◽

2012 ◽

Vol 96 (10) ◽

pp. 1577-1577 ◽

Cited By ~ 6

Author(s):

J. R. Lamichhane ◽

A. Fabi ◽

L. Varvaro

Keyword(s):

16S Rdna ◽

Bacterial Blight ◽

Central Italy ◽

Positive Control ◽

Negative Control ◽

Early Summer ◽

Nutrient Broth ◽

Universal Primers ◽

Bacterial Suspension ◽

Xanthomonas Arboricola

Hazelnut (Corylus avellana L.) is one of the most economically important tree crops in Italy. Xanthomonas arboricola pv. corylina (Xac) causes bacterial blight of hazelnut (4). During early summer 2010, a survey of three orchards (5 ha total) containing 4-year-old hazelnut trees (cv. Tonda di Giffoni) in Viterbo Province, Latium region, Italy, showed an 80 to 100% incidence of bacterial blight. Initially, water-soaked, necrotic spots were visible on leaves, fruit involucres, and shells, followed by lateral shoot dieback and development of cankers as longitudinal bark cracks on twigs, branches, and main trunks. Brown necrosis of the cambium was observed when bark tissue was removed. By late summer, necrosis had extended down main branches to the trunk, causing complete girdling of branches. Symptomatic tissues were collected from leaves, branches, and trunks, sections were surface-sterilized in 1% NaOCl for 1 min followed by two rinses in sterile distilled water (SDW, each for 1 min), and each section was then crushed in SDW. A loopful of the suspension was streaked onto yeast extract-dextrose-calcium carbonate agar medium (YDCA). Thirty six (12 from each type of tissue) yellow-mucoid, shiny, round bacterial colonies, each approximately 2 mm in diameter, were subcultured on YDCA. All strains were gram-negative and aerobic; negative for indole, lecithinase, urease, tyrosinase, and nitrate reduction; and positive for catalase, growth in 2% NaCl in nutrient broth, and growth at 35°C. All strains produced dark green pigment on succinate-quinate (SQ) medium. Inoculum of each of 15 isolates was prepared in nutrient broth, and washed cells from late log-phase cultures used to prepare a bacterial suspension of each isolate for inoculation of 2-year-old potted hazelnut plants cv. Tonda di Giffoni. A suspension of 106 CFU/ml for each isolate was sprayed onto leaves (10 ml/plant), and drops of inoculum were placed on wounds made on twigs with a sterile scalpel (0.10 μl/wound). For each isolate, three plants were inoculated per inoculation method. Inoculations with two reference strains of Xac (Xaco 1 from central Italy (3) and NCPPB 2896 from England) and SDW were performed on the same number of plants for positive and negative control treatments, respectively. Inoculated plants were maintained at 26 ± 1°C in a greenhouse. After 21 days, all inoculated plants had developed symptoms on leaves, while cankers developed on twigs after 40 days. Positive control plants developed the same symptoms, while negative control plants remained asymptomatic. Bacteria recovered from lesions on plants inoculated with the test strains or positive control strains had the same morphological and physiological characteristics as the original strains. No bacteria were recovered from negative control plants. Total DNA was extracted from bacterial suspensions and 16S rDNA amplified using universal primers (2). Sequences (GenBank Accession Nos. JQ861273, JQ861274, and JQ861275 for strains Xaco VT3 to VT5) had 99 to 100% identity with 16S rDNA sequences of Xac strains in GenBank. In Italy, Xac was reported by Petri in 1932 in Latium, and later in other regions on several hazelnut cultivars (1). However, to our knowledge, this is the first report of the disease causing severe damage in Italy. References: (1) M. Fiori et al. Petria 16:71, 2006. (2) J. R. Lamichhane et al. Plant Dis. 95:221, 2011. (3) J. R. Lamichhane et al. Acta Horticol.:In press. 2012. (4) OEPP/EPPO Bull. 179:179, 2004.

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Ekstrak Air Rumput Kebar (Biophytum petersianum Klotzch) sebagai Penghambat Perkembangan Telur Cacing Gastrointestinal Ruminansia Secara in Vitro

Jurnal Sain Veteriner ◽

10.22146/jsv.29297 ◽

2017 ◽

Vol 35 (1) ◽

pp. 102

Author(s):

Alnita Baaka ◽

Isti Widayati ◽

Noviyanti Noviyanti

Keyword(s):

Room Temperature ◽

Animal Health ◽

Animal Husbandry ◽

Economic Loss ◽

Positive Control ◽

Negative Control ◽

Zoonotic Diseases ◽

Dried Extract ◽

Descriptive Method

Gastrointestinal worm disease in cattle is scathing for the breeder’s as it lowers cattle’s productivity and inflict major economic loss. Furthermore, the interraction between cattle, man and the environment might bringout the risk of zoonotic diseases (diseases which can spread between man and animals). Kebar grass contains phytochemical elements such as alkaloid, saponin, tanin, glycoside and flavonoid. Those elements have the potentialas an alternate anthelmentic in the effort to utilize the endemic biodiversities of Papua. This research is performed at Faculty of Animal Husbandry Animal Health Laboratory and Faculty of Math and Natural Science MicrobiologyLaboratory of Universitas Papua, for 8 months. The leaves and the stems of the kebar grass were collected and stored at room temperature. The Kebar grass extract was divided into 3 parts, i.e. fresh extract, wind dried extractand sun dried extract. Fresh fecal samples were collected and examined with the native method. The samples where nematodes eggs are found is treated with kebar grass extract. As a comparison, positive control is made withoxfendazole and negative control is made nematodes larvae fertilization. Fecal cultures and grass extract mixture is left alone for 7 days in room temperature and examined under microscope with 400x fortification to see whetnerthere is any development of the nematodes’ eggs. The obstained data is analyzed by tabulation and descriptive method. The result is the samples mixed with kebar grass extract show no development of nematodes’ egg. As acomparison, the negative control which is observed with baerman method shows development of nematodes’ eggs. It can be concluded that the effect of kebar grass extract can deters nematodes’ egg development.

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Piglet Feed Contaminated with Aflatoxin B1 and Supplemented with an Anti-Mycotoxin Blend: Impact on Health and Zootechnical Performance

10.21203/rs.3.rs-630039/v1 ◽

2021 ◽

Author(s):

Lara Tarasconi ◽

Vanessa Dazuk ◽

Vitor Molosse ◽

Bruno Cecere ◽

Guilherme Deolindo ◽

...

Keyword(s):

Weight Gain ◽

Aflatoxin B1 ◽

Reductase Activity ◽

Animal Health ◽

Experimental Period ◽

Positive Control ◽

Negative Control ◽

The Other ◽

Control Group ◽

Negative Effects

Abstract The present study aimed to determine whether the inclusion of a mixture based on Saccharomyces cerevisiae lysate, zeolite, silicon dioxide, propylene glycol, Carduus marianus extract, soy lecithin, and carbonate in the piglet diet would be able to minimize the negative effects caused by feed with aflatoxin; a study focusing on animal health and performance. Seventy-two entire male piglets (7.35 ± 1.17 kg) weaned with an average of 26 days were used, allotted into four groups, with six repetitions (pens) per group and three piglets/pen. The treatments were as follows: NoAfla-NoAntiMyc - negative control (without aflatoxin); Afla-NoAntiMyc - positive control (500 ppb of aflatoxin); NoAfla-AntiMyc - 1000 mg/kg of anti-mycotoxin mixture; Afla-AntiMyc - 500 ppb aflatoxin + 1000 mg/kg anti-mycotoxin. We evaluated the performance (feed intake - FI, weight gain - WG and feed:gain ratio- FGR) andblood variables (10 days between samples). On day 32, six pigs from each group (a total of 24 pigs) were slaughtered to collect fragments of the liver, intestine, and spleen for analysis of oxidants/antioxidants and histology. It was observed that piglets in the positive control group (Afla-NoAntiMyc) had lower body weight and weight gain when compared to the other groups during the experimental period. Also, piglets from Afla-NoAntiMyc consumed less feed between days 1 to 20 and 1 to 30 compared to NoAfla-NoAntiMyc. A considerable increase in liver enzymes aspartate aminotransferase and alanine aminotransferase in the piglets' serum of Afla-NoAntiMyc compared to other treatments, which indicates that the anti-aflatoxin blend worked. Fewer neutrophils and an increase in monocyte were observed in the piglets of Afla-NoAntiMyc compared to NoAfla-NoAntiMyc; changes that were not seen in the animals of Afla-AntiMyc. We did not see any change in the oxidant and antioxidant status in the piglet serum during the experimental period; different from what happened in Afla-NoAntiMyc piglets in the liver (higher glutathione reductase activity and levels of reactive oxygen species - ROS), in the spleen (higher levels of ROS) and the intestine/jejunum (reduction of nitrate/nitrite levels - NOx). Intestinal morphometry revealed that piglets from Afla-NoAntiMyc had higher villus height than the other groups, while the folded size was smaller in this group. The crypt depth was greater in the intestine of piglets in both treatments that consumed aflatoxin. In general, it is concluded that the consumption of aflatoxin B1 by piglets has negative impacts on the health and, consequently, the animals' performance; however, supplementation of the contaminated feed with an anti-mycotoxin blend was able to protect the piglets, minimizing the negative problems caused by the mycotoxin.

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Peningkatan Mutu Fisiologis dan Daya Simpan Benih serta Ketahanan Patogen dan Agen Hayati pada Benih Padi Berpelapis

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) ◽

10.24831/jai.v44i3.12755 ◽

2017 ◽

Vol 44 (3) ◽

pp. 242

Author(s):

Tantri Palupi ◽

Satriyas Ilyas ◽

Muhammad Machmud ◽

Dan Eny Widajati

Keyword(s):

Seed Quality ◽

Storage Period ◽

Germination Percentage ◽

Positive Control ◽

Negative Control ◽

Biological Agents ◽

Xanthomonas Oryzae ◽

Seed Coating ◽

Arabic Gum ◽

Main Plot

ABSTRACT Xanthomonas oryzae pv. oryzae (Xoo), is a seedborne pathogen causing bacterial leaf blight (BLB) disease, and reduces the quality of seed and rice production. One of the efforts to control the BLB disease and to improve the quality Xoo infected seeds is the seed coating technique enriched with biological agents. The experiment was aimed to study the effect of coating on seed quality and storage life, as well as the Xoo and biological agents resistence (P. diminuta A6 and B. subtilis 5/B) on the seeds. The experiment was carried out from August 2011 to March 2012, using a split plot design with four replications. The main plot was storage period, i.e. 0, 1, 2, 3, 4, 5, 6, and 7 months. The sub plot was seed coating treatment consisted of negative control (healthy seed); positive control (seeds contaminated with Xoo); seed infested with biological agens; alginate 3% + 1% peat + biological agents; arabic gum 3% + 1% gypsum + biological agents; CMC 1.5% + 1% talc + biological agents; and bactericide streptomycin sulfat 20%. The coated seeds were stored an air-conditioned room (18-20 °C, RH 48-50%). The results showed that the treatments were able to maintain seeds quality during storage, i.e. germination percentage, uniformity percentage, and vigor index, better than those of the positive control. The P. diminuta A6 was still presence (0.08 x 106 cfu mL-1) in seeds coated after 7 month storage, and the B. subtilis 5/B was still presence (0.07 x 106 cfu mL-1) up to 6 month storage with 3% arabic gum + 1% gypsum + biological agents. Keywords: Bacillus subtilis 5/B, Pseudomonas diminuta A6, seed quality, storage space, Xanthomonas oryzae pv. oryzae

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Effects of Disturbance on Silver Sagebrush Communities in Dry Mixed-Grass Prairie

Ecological Restoration ◽

10.3368/er.31.3.274 ◽

2013 ◽

Vol 31 (3) ◽

pp. 274-282

Author(s):

L. K. Hickman ◽

P. A. Desserud ◽

B. W. Adams ◽

C. C. Gates

Keyword(s):

Mixed Grass Prairie ◽

Mixed Grass

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Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

Jurnal Ilmu Kedokteran ◽

10.26891/jik.v9i2.2015.71-77 ◽

2017 ◽

Vol 9 (2) ◽

pp. 71

Author(s):

Nurhasanah Nurhasanah ◽

Fauzia Andrini ◽

Yulis Hamidy

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Allium Sativum ◽

Fungicidal Activity ◽

Positive Control ◽

Negative Control ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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The Comparison of Ostecyte in the Pressure area and Tension area on Tooth Movement Because of Hyperbaric Oxygen Therapy

DENTA ◽

10.30649/denta.v12i1.162 ◽

2018 ◽

Vol 12 (1) ◽

pp. 34

Author(s):

Arya Barahmanta ◽

Muhammad Faizal Winaris ◽

Pambudi Raharjo

Keyword(s):

Hyperbaric Oxygen ◽

Oxygen Therapy ◽

Tooth Movement ◽

Bone Remodelling ◽

Orthodontic Tooth Movement ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Pressure Area

Background: Orthodontic tooth movement is a interaction prosess of resorption and deposition of bone remodeling. Orthodontic tooth movement by mechanical strength causes changes in alveolar bone. Osteocyte is an essential cell to respond bone remodelling. Hyperbaric Oxygen Therapy affects production of osteocyte because it can release Reactive Oxygen Species (ROS) and Nitrid Oxide (NO). Purpose: To determine the difference number of osteocyte in pressure and tension area during tooth movement by adjuvant of Hyperbaric Oxygen 2,4 ATA during 7 days starting on day 8 to day 14. Materials and Methods: This research used Completery Randomized Control Group Post Test Only Design. 36 cavia cobaya (male) were divided into 3 groups randomly : the negative control groups, positive control group, and treatment group. Preparat staining used Hematoxylin Eosin (HE) and calculated on microscop 1000x with 20 field of view. Data analyses used one way ANOVA and LSD test then compared each area by using paired T test. Result: The data showed that the treatment group (P=10,67) tension area has the highest number of osteocyte than negative control group (K-=3,67), positive control (K+=7,42). In the pressure area showed that negative control group (K-=5,00) has the highest than positive control group (K+=3,83) and treatment (P=3,25). Conclusion: Therapy HBO 2,4 ATA 7 days starting on day 8 to day 14 is could increase osteocyte in the tissue to stimulate process of bone remodelling.<pre> </pre>Keywords: Hyperbaric Oxygen, Tooth movement, Bone remodeling, Osteocyte Correspondence: Arya Brahmanta, Department of Orthodonty, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, Email: <a href="mailto:[email protected]">arya.brahmanta@hangtuah.ac.id</a>

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UJI AKTIVITAS HEPATOPROTEKTOR KOMBINASI EKSTRAK ETANOL DAUN PANDAN WANGI (Pandanus amaryllifolius roxb) DAN KAYU MANIS (Cinnamomum burmanii) TERHADAP KADAR MDA YANG DIINDUKSI PARASETAMOL

Jurnal Kesehatan Kusuma Husada ◽

10.34035/jk.v12i1.580 ◽

2020 ◽

pp. 68-73

Author(s):

Yuni Asri Mulatsih Agami ◽

Eka Wisnu Kusuma

Keyword(s):

Oxidative Stress ◽

Ethanol Extract ◽

Optimal Dose ◽

Experimental Methods ◽

Positive Control ◽

Negative Control ◽

Male Rats ◽

Negative Group ◽

Ic50 Value ◽

Dose Combination

Kasus penyakit hati semakin meningkat seiring penggunaan senyawa hepatotoksin salah satunya karena penggunaan parasetamol dengan dosis berlebih. Hal tersebut dapat meningkatkan produksi radikal bebas sehingga memicu terjadinya stress oksidatif yang dapat menimbulkan kerusakan jaringan yang ditandai dengan peningkatan kadar Malondialdehyde (MDA). Stress oksidatif dapat diatasi dengan antioksidan dari berbagai tanaman. Kulit kayu manis memiliki aktivitas antioksidan dengan nilai IC50 53ppm dan daun pandan wangi 39,7% Penelitian ini bertujuan untuk mengetahui aktivitas kombinasi ekstrak etanol daun pandan wangi dan kayu manis dalam menurunkan kadar MDA. tikus yang diinduksi parasetamol. Penelitian menggunakan metode eksperimental, dilakukan selama 9 hari dengan 30 ekor tikus jantan dibagi menjadi 6 Kelompok, yaitu: Normal diberi aquadest, Kontrol Positif diberi silimarin 100 mg/kgBB, Kontrol Negatif diberi CMC-Na 0,05%, serta 3 kelompok lainnya diberi kombinasi ekstrak daun pandan wangi:kayu manis berturut-turut dosis I (25:75), dosis II (50:50), dosis III (75:25). Semua kelompok diinduksi parasetamol 2,5 g/kgBB pada hari ke-7 setelah 30 menit perlakuan, kecuali kelompok normal. Pada hari ke 9 dilakukan pengukuran kadar MDA dengan metode TBARs menggunakan spektrofotometri. Pemberian kombinasi ekstrak etanol daun pandan wangi dan kayu manis dapat menurunkan kadar MDA dengan kombinasi dosis yang paling optimal adalah 75:25 berdasarkan statistik dengan nilai signifikan 0,000<0,05 dibandingkan dengan kelompok negatif. Cases of liver disease have increased with the use of hepatotoxin compounds, one of which is due to the use of paracetamol with excessive doses. This can increase the production of free radicals so that it triggers oxidative stress which can cause tissue damage which is characterized by increased levels of Malondialdehyde (MDA). Oxidative stress can be overcome with antioxidants from various plants. Cinnamomum burmanii has antioxidant activity with IC50 value of 53ppm and Pandanus amarrylifolius 39.7%. This study aims to determine the combined activity of ethanol extract of Pandanus amarrylifolius and Cinnamomum burmanii in reducing MDA levels. Paracetamol-induced rats. Research using experimental methods, conducted for 9 days with 30 male rats divided into 6 groups, namely: Normal given aquadest, Positive Control were given silimarin 100 mg / kgBB, Negative Control was given CMC-Na 0.05%, and 3 other groups were given a combination of Pandanus amarrylifolius extract: Cinnamomum burmanii dose I (25:75), dose II (50:50), dose III (75:25). All groups induced paracetamol 2.5 g / kgBB on the 7th day after 30 minutes of treatment, except the normal group. On the 9th day MDA levels were measured using the TBARs method using spectrophotometry. Giving a combination of Pandanus amarrylifolius and Cinnamomum burmanii ethanol extract can reduce MDA levels with the most optimal dose combination is 75:25 based on statistics with a significant value of 0,000<0.05 compared with the negative group.

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