scholarly journals PSXV-26 Coated and non-coated steroidal implants containing trenbolone acetate and estradiol benzoate on adipogenic gene expression of beef steers.

2018 ◽  
Vol 96 (suppl_3) ◽  
pp. 240-241
Author(s):  
J Kim ◽  
Z Smith ◽  
B Johnson
Keyword(s):  
Gene Expression ◽  
Estradiol Benzoate ◽  
Beef Steers ◽  
Trenbolone Acetate ◽  
Adipogenic Gene

scholarly journals PSIII-8 Impact of SYNOVEX® ONE GRASS implantation during suckling on pre-weaning calf growth performance

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 239-239
Author(s):  
Shane Newton ◽  
Payton Dahmer ◽  
Larry Corah ◽  
Keith Harmoney ◽  
John Jaeger ◽  
...  
Keyword(s):  
Growth Performance ◽  
Random Effect ◽  
Cost Effective ◽  
Recent Decade ◽  
Estradiol Benzoate ◽  
Beef Steers ◽  
Weaning Weight ◽  
Trenbolone Acetate ◽  
Calf Growth ◽  
Randomized Design

Abstract Historic data supports the use of pre-weaning implants to maximize beef calf weaning weight, but their use has declined over the recent decade. A total of 96 beef steers (initially 85.2 kg BW and > 45 d of age) were used in a 168-d study to evaluate the effects of Synovex C® implantation during suckling on pre-weaning calf growth performance. At initial processing, calves were individually weighed and allotted in a completely randomized design to one of two treatments: 1) not implanted (control) or 2) implanted with 150 mg of trenbolone acetate and 21 mg of estradiol benzoate (SYNOVEX® ONE GRASS, Zoetis, Kalamazoo, MI). All calves were weighed on d 0 (implantation), 83, 126 and 168 (weaning), with ADG calculated for each period. Data were analyzed using the GLIMMIX procedure of SAS (v9.4, Cary, NC). There were 48 replicates per treatment, with grazing location as a random effect. While there were no detected differences in BW during the initial phases (P > 0.10), implanted calves tended to have greater (P = 0.100) greater weaning weight than non-implanted calves (279 vs. 265 kg, respectively). Calves implanted with SYNOVEX® ONE GRASS had 0.8, 0.12, and 0.16 kg/d greater (P < 0.05) ADG than those that were not implanted in all three phases. This resulted in an average of 0.10 kg/d greater (P = 0.002) ADG in implanted calves overall compared to the control. In conclusion, this research showed significant increases in suckling calf ADG in implanted calves compared to their non-implanted counterparts, which led to a tendency for 14-kg greater weaning weights. Additional research is warranted to consider the post-weaning growth and impact of re-implanting, but this data reinforces previous literature that suckling calf implantation continues to be cost effective for cow-calf producers.

Anabolic payout of terminal implant alters adipogenic gene expression of the longissimus muscle in beef steers

2017 ◽  
Vol 95 (3) ◽  
pp. 1197
Author(s):  
Z. K. Smith ◽  
K. Y. Chung ◽  
S. L. Parr ◽  
B. J. Johnson
Keyword(s):  
Gene Expression ◽  
Beef Steers ◽  
Longissimus Muscle ◽  
Adipogenic Gene

scholarly journals 179 Using a 100% Dried Distillers Grains Cube as a Supplement for Steers Grazing Mixed Grass Prairie in Oklahoma

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 46-46
Author(s):  
Zane N Grigsby ◽  
Paul A Beck ◽  
Stacey A Gunter
Keyword(s):  
Animal Health ◽  
Late Summer ◽  
Estradiol Benzoate ◽  
Positive Control ◽  
Negative Control ◽  
Early Summer ◽  
Trenbolone Acetate ◽  
Mixed Grass Prairie ◽  
Mixed Grass ◽  
Main Plot

Abstract This research was conducted to determine effects of supplementation and implanting on BW gain by steers grazing mixed grass prairie (n = 12 pastures, 19.9 ± 0.7 ha) in northwest Oklahoma. Three main plot treatments were: 1) Negative Control (NC), no supplementation, 2) Positive Control (PC), supplemented with DDGS cubes, 1.8 kg/steer on alternate days in late summer, 3) High Supplement (HS), 1/3 increase in stocking rate with 0.75% BW supplemental DDGS cubes all season. Steers (n = 125, BW = 223.1 ± 23.2 kg) were stocked at 2.2 ha/steer for PC and NC, 1.3 ha/steer for HS. Grazing was from May 17 – September 27 (132 d). All steers were implanted with 200 mg progesterone and 20 mg estradiol benzoate (SYN, Synonvex S, Zoetis Animal Health) on May 17. On July 18 three reimplant treatments were applied: 1) no reimplant; 2) SYN; or 3) 40 mg trenbolone acetate and 8 mg estradiol (Revalor G, Merck Animal Health). Data were analyzed using the PROC MIXED in SAS as a split-plot experimental design. In early summer HS had 0.26 kg greater (P < 0.01) ADG than NC and PC. Late summer gains of PC were 0.33 kg/d more (P ≤ 0.01) than NC; and HS gained 0.49 and 0.16 kg/day more (P ≤ 0.04) than NC and PC, respectively. Gain per hectare for PC (46 kg/ha) were greater (P < 0.01) than NC (35 kg/ha) and more than doubled (P < 0.01) with HS (89 kg/ha). Reimplanting had no effect on ADG (P ≥ 0.28). Late season supplementation with PC resulted in supplemental efficiency of 2.7 kg supplement/kg added gain compared with NC. Increased stocking rates with season long supplementation in HS resulted in supplemental efficiency of 3.8 kg supplement/kg added gain per hectare. Based on these data, a 100% DDGS cube is an effective supplement option to increase BW gain during the late summer or increase carrying capacity and gain during the summer grazing period in northwestern Oklahoma.

scholarly journals Anti-Obesity and Anti-Adipogenic Effects of Chitosan Oligosaccharide (GO2KA1) in SD Rats and in 3T3-L1 Preadipocytes Models

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 331
Author(s):  
Jung-Yun Lee ◽  
Tae Yang Kim ◽  
Hanna Kang ◽  
Jungbae Oh ◽  
Joo Woong Park ◽  
...  
Keyword(s):  
Gene Expression ◽  
Body Weight ◽  
Density Lipoprotein ◽  
Treated Group ◽  
Control Group ◽  
Chitosan Oligosaccharide ◽  
Sd Rats ◽  
Adipogenic Gene

Excess body weight is a major risk factor for type 2 diabetes (T2D) and associated metabolic complications, and weight loss has been shown to improve glycemic control and decrease morbidity and mortality in T2D patients. Weight-loss strategies using dietary interventions produce a significant decrease in diabetes-related metabolic disturbance. We have previously reported that the supplementation of low molecular chitosan oligosaccharide (GO2KA1) significantly inhibited blood glucose levels in both animals and humans. However, the effect of GO2KA1 on obesity still remains unclear. The aim of the study was to evaluate the anti-obesity effect of GO2KA1 on lipid accumulation and adipogenic gene expression using 3T3-L1 adipocytes in vitro and plasma lipid profiles using a Sprague-Dawley (SD) rat model. Murine 3T3-L1 preadipocytes were stimulated to differentiate under the adipogenic stimulation in the presence and absence of varying concentrations of GO2KA1. Adipocyte differentiation was confirmed by Oil Red O staining of lipids and the expression of adipogenic gene expression. Compared to control group, the cells treated with GO2KA1 significantly decreased in intracellular lipid accumulation with concomitant decreases in the expression of key transcription factors, peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding protein alpha (CEBP/α). Consistently, the mRNA expression of downstream adipogenic target genes such as fatty acid binding protein 4 (FABP4), fatty acid synthase (FAS), were significantly lower in the GO2KA1-treated group than in the control group. In vivo, male SD rats were fed a high fat diet (HFD) for 6 weeks to induced obesity, followed by oral administration of GO2KA1 at 0.1 g/kg/body weight or vehicle control in HFD. We assessed body weight, food intake, plasma lipids, levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) for liver function, and serum level of adiponectin, a marker for obesity-mediated metabolic syndrome. Compared to control group GO2KA1 significantly suppressed body weight gain (185.8 ± 8.8 g vs. 211.6 ± 20.1 g, p < 0.05) with no significant difference in food intake. The serum total cholesterol, triglyceride, and low-density lipoprotein (LDL) levels were significantly lower in the GO2KA1-treated group than in the control group, whereas the high-density lipoprotein (HDL) level was higher in the GO2KA1 group. The GO2KA1-treated group also showed a significant reduction in ALT and AST levels compared to the control. Moreover, serum adiponectin levels were significantly 1.5-folder higher than the control group. These in vivo and in vitro findings suggest that dietary supplementation of GO2KA1 may prevent diet-induced weight gain and the anti-obesity effect is mediated in part by inhibiting adipogenesis and increasing adiponectin level.

PSX-B-5 Effect of replacement of dry-rolled corn with un-processed rye on growth performance, efficiency of dietary net energy utilization, and carcass traits of finishing heifers

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 458-459
Author(s):  
Keith M Buckhaus ◽  
Warren C Rusche ◽  
Zachary K Smith
Keyword(s):  
Body Weight ◽  
Growth Performance ◽  
Block Design ◽  
Average Daily Gain ◽  
Estradiol Benzoate ◽  
Energy Utilization ◽  
Net Energy ◽  
Final Body Weight ◽  
Trenbolone Acetate ◽  
Finishing Diets

Abstract Continental × British beef heifers were used in a randomized complete block design experiment to evaluate the effects of replacing dry-rolled corn with unprocessed rye on growth performance, efficiency of dietary net energy (NE) utilization, and carcass trait responses in finishing heifers. Heifers (n = 56; 433 ± 34.0 kg) were transported 241 km from a regional sale barn to the Ruminant Nutrition Center in Brookings, SD. Heifers were blocked by weight grouping and then allotted to pens (n = 7 heifers/pen and 4 pens/treatment). Treatments included a finishing diet that contained 60% grain (DM basis) as dry-rolled corn (DRC) or unprocessed rye grain (RYE). On d 14, heifers were consuming the final diet and were implanted with 200 mg of trenbolone acetate and 28 mg of estradiol benzoate (Synovex-Plus, Zoetis, Parsippany, NJ). RYE heifers had decreased (P ≤ 0.01) final body weight, average daily gain, and gain efficiency; but tended (P = 0.08) to have a greater dry matter intake compared to DRC. RYE had decreased (P ≤ 0.01) observed dietary NE and decreased (P ≤ 0.01) observed-to-expected dietary NE ratio for maintenance and gain compared to DRC. Dressing percentage, 12th rib fat thickness, ribeye area, and the distribution of USDA yield and quality grades were not altered (P ≥ 0.12) by diet. Hot carcass weight, yield grade, estimated empty body fat (EBF), and body weight at 28% EBF decreased (P ≤ 0.02) and retail yield increased (P= 0.01) in RYE compared to DRC. These data indicate that unprocessed rye is a palatable feed ingredient for inclusion in finishing diets for beef cattle and that rye inclusion only minimally influences carcass quality. The feeding value of unprocessed rye is considerably less (21.4%) than that of dry-rolled corn using current standards and approximately 91% of the NE value of processed rye.

Analysis of a tissue-specific enhancer: ARF6 regulates adipogenic gene expression

1992 ◽  
Vol 12 (3) ◽  
pp. 1202-1208
Author(s):  
R A Graves ◽  
P Tontonoz ◽  
B M Spiegelman
Keyword(s):  
Gene Expression ◽  
Cultured Cells ◽  
Mutational Analysis ◽  
Cell Types ◽  
Specific Gene ◽  
Enhancer Activity ◽  
Cis Acting ◽  
Specific Gene Expression ◽  
Nuclear Extracts ◽  
Adipogenic Gene

The molecular basis of adipocyte-specific gene expression is not well understood. We have previously identified a 518-bp enhancer from the adipocyte P2 gene that stimulates adipose-specific gene expression in both cultured cells and transgenic mice. In this analysis of the enhancer, we have defined and characterized a 122-bp DNA fragment that directs differentiation-dependent gene expression in cultured preadipocytes and adipocytes. Several cis-acting elements have been identified and shown by mutational analysis to be important for full enhancer activity. One pair of sequences, ARE2 and ARE4, binds a nuclear factor (ARF2) present in extracts derived from many cell types. Multiple copies of these elements stimulate gene expression from a minimal promoter in preadipocytes, adipocytes, and several other cultured cell lines. A second pair of elements, ARE6 and ARE7, binds a separate factor (ARF6) that is detected only in nuclear extracts derived from adipocytes. The ability of multimers of ARE6 or ARE7 to stimulate promoter activity is strictly adipocyte specific. Mutations in the ARE6 sequence greatly reduce the activity of the 518-bp enhancer. These data demonstrate that several cis- and trans-acting components contribute to the activity of the adipocyte P2 enhancer and suggest that ARF6, a novel differentiation-dependent factor, may be a key regulator of adipogenic gene expression.

Acute exercise regulates adipogenic gene expression in white adipose tissue

2016 ◽  
Vol 33 (4) ◽  
pp. 381-391 ◽  
Author(s):  
Yanyan Shen ◽  
Huiqiao Zhou ◽  
Wanzhu Jin ◽  
Hyuek Jong Lee
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
White Adipose Tissue ◽  
Acute Exercise ◽  
Adipogenic Gene
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