scholarly journals Biotechnology: An Advanced Tool for Crop Improvement

2019 ◽  
pp. 1-11 ◽  
Author(s):  
Tinee Adlak ◽  
Sushma Tiwari ◽  
M. K. Tripathi ◽  
Neha Gupta ◽  
Vinod Kumar Sahu ◽  
...  
Keyword(s):  
Molecular Breeding ◽  
Genetically Modified Organisms ◽  
Somatic Hybrids ◽  
Crop Improvement ◽  
Breeding Methods ◽  
Biotic And Abiotic Stress ◽  
New Era ◽  
Conventional Methods ◽  
Generation Sequencing ◽  
Selection Of

Plant breeding is mainly concerned with genetic improvement of crops through hybridization, screening and selection of advance lines. The conventional methods give advance varieties with desirable traits but take consume more time (6 to 12 years) to achieve. Biotechnology tools makes breeding methods more advance by reducing the time to get improved varieties. Other than conventional methods varietal advancement can be achieved by applying plant tissue culture, transgenic approaches and molecular breeding methods. Crop improvement by using biotechnology approaches is mostly concerned with protoplast fusion to get somatic hybrids, gene transfer to get genetically modified organisms and use of DNA markers to select trait of interests. Variety with improved biotic and abiotic stress resistance can be developed in less time and more accuracy using recent biotechnological approaches. Several advance tools are being utilized for that purpose including, nanotechnology, bioinformatics tools offers new era of genomics assisted molecular breeding. Next Generation Sequencing and high throughput genotyping approaches are increasing efficiency and output of biotechnological tools in agriculture. Current review focused on overview of biotechnological tools being applied for crop improvement.

scholarly journals Current achievements and future prospects in the genetic breeding of chrysanthemum: a review

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Jiangshuo Su ◽  
Jiafu Jiang ◽  
Fei Zhang ◽  
Ye Liu ◽  
Lian Ding ◽  
...  
Keyword(s):  
Molecular Breeding ◽  
Abiotic Stress Tolerance ◽  
Mutation Breeding ◽  
Chrysanthemum Morifolium ◽  
Postharvest Quality ◽  
Breeding Methods ◽  
Biotic And Abiotic Stress ◽  
Efficient Manner ◽  
Phenotypic Information ◽  
Cost Efficient

Abstract Chrysanthemum (Chrysanthemum morifolium Ramat.) is a leading flower with applied value worldwide. Developing new chrysanthemum cultivars with novel characteristics such as new flower colors and shapes, plant architectures, flowering times, postharvest quality, and biotic and abiotic stress tolerance in a time- and cost-efficient manner is the ultimate goal for breeders. Various breeding strategies have been employed to improve the aforementioned traits, ranging from conventional techniques, including crossbreeding and mutation breeding, to a series of molecular breeding methods, including transgenic technology, genome editing, and marker-assisted selection (MAS). In addition, the recent extensive advances in high-throughput technologies, especially genomics, transcriptomics, proteomics, metabolomics, and microbiomics, which are collectively referred to as omics platforms, have led to the collection of substantial amounts of data. Integration of these omics data with phenotypic information will enable the identification of genes/pathways responsible for important traits. Several attempts have been made to use emerging molecular and omics methods with the aim of accelerating the breeding of chrysanthemum. However, applying the findings of such studies to practical chrysanthemum breeding remains a considerable challenge, primarily due to the high heterozygosity and polyploidy of the species. This review summarizes the recent achievements in conventional and modern molecular breeding methods and emerging omics technologies and discusses their future applications for improving the agronomic and horticultural characteristics of chrysanthemum.

An Insight into Oligopeptide Transporter 3 (OPT3) family proteins

2020 ◽  
Vol 27 ◽  
Author(s):  
Fırat Kurt
Keyword(s):  
Stress Responses ◽  
Chelating Agent ◽  
Biological Processes ◽  
Biotic And Abiotic Stress ◽  
Oligopeptide Transporter ◽  
Binding Residues ◽  
Fe Homeostasis ◽  
Proteins Interactions ◽  
Insight Into ◽  
Selection Of

: Oligopeptide transporter 3 (OPT3) proteins are one of the subsets of OPT clade, yet little is known about these transporters. Therefore, homolog OPT3 proteins in several plant species were investigated and characterized using bioinformatical tools. Motif and co-expression analyses showed that OPT3 proteins may be involved in both biotic and abiotic stress responses as well as growth and developmental processes. AtOPT3 usually seemed to take part in Fe homeostasis whereas ZmOPT3 putatively interacted with proteins involved in various biological processes from plant defense system to stress responses. Glutathione (GSH), as a putative alternative chelating agent, was used in the AtOPT3 and ZmOPT3 docking analyses to identify their putative binding residues. The information given in this study will contribute to the understanding of OPT3 proteins’ interactions in various pathways and to the selection of potential ligands for OPT3s.

scholarly journals Evolution and Application of Genome Editing Techniques for Achieving Food and Nutritional Security

2021 ◽  
Vol 22 (11) ◽  
pp. 5585
Author(s):  
Sajid Fiaz ◽  
Sunny Ahmar ◽  
Sajjad Saeed ◽  
Aamir Riaz ◽  
Freddy Mora-Poblete ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Food Security ◽  
Plant Breeding ◽  
Genome Editing ◽  
Crop Improvement ◽  
Hybrid Seed Production ◽  
Biotic And Abiotic Stress ◽  
Transcription Activator ◽  
Protein System ◽  
Breeding Techniques

A world with zero hunger is possible only through a sustainable increase in food production and distribution and the elimination of poverty. Scientific, logistical, and humanitarian approaches must be employed simultaneously to ensure food security, starting with farmers and breeders and extending to policy makers and governments. The current agricultural production system is facing the challenge of sustainably increasing grain quality and yield and enhancing resistance to biotic and abiotic stress under the intensifying pressure of climate change. Under present circumstances, conventional breeding techniques are not sufficient. Innovation in plant breeding is critical in managing agricultural challenges and achieving sustainable crop production. Novel plant breeding techniques, involving a series of developments from genome editing techniques to speed breeding and the integration of omics technology, offer relevant, versatile, cost-effective, and less time-consuming ways of achieving precision in plant breeding. Opportunities to edit agriculturally significant genes now exist as a result of new genome editing techniques. These range from random (physical and chemical mutagens) to non-random meganucleases (MegaN), zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein system 9 (CRISPR/Cas9), the CRISPR system from Prevotella and Francisella1 (Cpf1), base editing (BE), and prime editing (PE). Genome editing techniques that promote crop improvement through hybrid seed production, induced apomixis, and resistance to biotic and abiotic stress are prioritized when selecting for genetic gain in a restricted timeframe. The novel CRISPR-associated protein system 9 variants, namely BE and PE, can generate transgene-free plants with more frequency and are therefore being used for knocking out of genes of interest. We provide a comprehensive review of the evolution of genome editing technologies, especially the application of the third-generation genome editing technologies to achieve various plant breeding objectives within the regulatory regimes adopted by various countries. Future development and the optimization of forward and reverse genetics to achieve food security are evaluated.

scholarly journals Global transcriptional differences in myokine and inflammatory genes in muscle of mature steer progeny are related to maternal lactation diet and muscle composition

2018 ◽  
Vol 50 (10) ◽  
pp. 884-892
Author(s):  
T. M. Casey ◽  
J. F. Walker ◽  
K. Bhide ◽  
J. Thimmapuram ◽  
J. P. Schoonmaker
Keyword(s):  
Inflammatory Cytokines ◽  
Control Diet ◽  
Muscle Composition ◽  
Dried Distillers Grains ◽  
Maintenance Factors ◽  
Transcriptomic Signature ◽  
Lactation Phase ◽  
Differentiation Pathways ◽  
Generation Sequencing ◽  
Selection Of

Steer progeny suckled by cows fed a dried distillers grains and solubles (DDGS) diet the first 3 mo of lactation were heavier during feedlot finishing and had significantly lower marbling and larger longissimus muscles than steers suckled by cows fed a control diet (CON). These differences were profound in that progeny were managed and fed identically from weaning until finishing, and findings suggest that the suckling period established the developmental program of muscle composition. Here transcriptomes of longissimus muscle were measured by next-generation sequencing to investigate whether there were any developmental clues to the differences in marbling scores and muscle content between steers suckled by DDGS ( n = 5) vs. control (CON; n = 5) diet-fed cows during lactation. There were 809 genes differentially expressed ( P-adj<0.1) between CON and DDGS muscle. Of these 636 were upregulated and 173 downregulated in DDGS relative to CON. Overall the DDGS vs. CON muscle transcriptomic signature was promyogenic and antiadipogenic. In particular, myokines/satellite cell maintenance factors were found among upregulated (LIF, CNTF, FGFB1, EPHB1) genes. The antiadipogenic signature was typified by the upregulation of anti-inflammatory cytokines and receptors (IL1RAP, IL1RL2, IL13RA2, IL1F10), and downregulation of expression of inflammation/inflammatory cytokines and receptor (TNF, IL6R, CXCL9), which suggests a selection of differentiation pathways away from adipogenic line. The upregulation of TGFB, SPP1, and INHBA supports selection of fibroblast lineage of cells. Thus, the lactation phase of production can effect meat quality by affecting transcriptional signatures that favor myogenesis and depress inflammation.

scholarly journals Advances in genetics and molecular breeding of three legume crops of semi-arid tropics using next-generation sequencing and high-throughput genotyping technologies

2012 ◽  
Vol 37 (5) ◽  
pp. 811-820 ◽  
Author(s):  
Rajeev K Varshney ◽  
Himabindu Kudapa ◽  
Manish Roorkiwal ◽  
Mahendar Thudi ◽  
Manish K Pandey ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
High Throughput ◽  
Molecular Breeding ◽  
Next Generation ◽  
Legume Crops ◽  
Generation Sequencing ◽  
Semi Arid

scholarly journals Development of KASP Markers for Germplasm Characterization and Fingerprinting Identification of Broccoli in China

2020 ◽  
Author(s):  
Yusen Shen ◽  
Jiansheng Wang ◽  
Huifang Yu ◽  
Xiaoguang Sheng ◽  
Zhenqing Zhao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Marker Assisted Selection ◽  
Genetic Relationships ◽  
Cost Effective ◽  
Germplasm Characterization ◽  
2D Barcode ◽  
New Type ◽  
Generation Sequencing ◽  
Kasp Markers ◽  
Selection Of

Abstract Background: Broccoli (Brassica oleracea var. italica) is a vegetable widely cultivated in China. Many new-type broccoli cultivars were bred and developed by Chinese breeders during the recent three decades. However, the broccoli cultivar nomenclature and detailed information of genetic relationships among broccoli germplasms are unclear. Results: The present study identified millions of SNPs by next-generation sequencing of 23 representative broccoli lines. Through several steps of selection, 100 SNPs were successfully converted into KASP markers, and used to evaluate the genetic diversity, genetic relationship, and population structure of 392 broccoli accessions, which represent the mainly broccoli breeding materials in China. The initial, introduced and improved accessions were well clustered, though some accessions were overlapped between groups, probably reflecting the fact that breeding activities led to genetic similarities. To make the KASP genotyping more efficient and cost-effective, 25 of the 100 KASPs were selected for fingerprinting of all accessions, and the 2D barcode contained fingerprinting information were generated for elite varieties. Conclusion: The KASP markers developed in this study provided an efficient way for germplasm characterization, DNA fingerprinting, seed purity identification, and marker-assisted selection of broccoli in China.

scholarly journals Theoretical justification of the possibility of selection perspective individuals in variety populations of self-pollinators on the example of soybean

2021 ◽  
Vol 186 (2) ◽  
pp. 31-40
Author(s):  
V.M. Lukomets ◽  
◽  
S.V. Zelentsov ◽  
E.V. Moshnenko ◽  
◽  
...  
Keyword(s):  
F1 Hybrids ◽  
Individual Selection ◽  
Breeding Methods ◽  
Morphometric Traits ◽  
Phenotypic Differences ◽  
Soybean Cultivars ◽  
Oil Crops ◽  
Experimental Base ◽  
Modified Formula ◽  
Selection Of

Breeding practice shows that soybean cultivars developed by synthetic breeding methods are submitted for the state variety testing in the F10–F11 generation. But the newly bred cultivars are not completely homozygous. The studies were related to the determination of the frequencies of formation of atypical and suitable for selection promising individuals in soybean cultivars developed by synthetic breeding methods. The studies were carried out in 2019–2020 at the central experimental base of V.S. Pustovoit All-Russian Research Institute of Oil Crops, Krasnodar. In the experiments, we used cultivars of our own breeding: Selena, Puma, Vita, Irbis, Bars, Barguzin and Sayana with a total age of 11–15 years from the year F1 hybrids were obtained. To confirm the practical possibility of isolating individuals differing from the phenotypic varietal norm in varietal populations, a complete examination of the crops of all studied soybean cultivars was carried out. In the fields of all cultivars, individuals were identified that differed from the varietal norm phenotypically. Mostly, the isolated individuals were distinguished by an increased plant height, a more powerful habit, an increased number of beans per plant, and resistance to lodging. The facts of the detection of atypical individuals with improved morphological traits in soybean cultivars of different ages confirm the possibility of individual selection of plants in varietal populations based on morphometric traits. The statistical dynamics of a decrease in heterozygous individuals in increasing generations in a hybrid self-pollinating population in terms of the number of paired genes, by which the parental forms can hypothetically differ, were calculated using the modified formula of S. Borojević (1984). It was found that the frequency of formation of phenotypically different individuals in varietal populations of soybeans depends on the total age of the cultivar. The frequency of the formation of morphologically different individuals decreases with an increase in the number of generations of the cultivar. Individual selection of individuals with positive phenotypic differences from the varietal norm can be recommended as an additional source of promising and practically homozygous starting material for accelerated analytical breeding of soybean.

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