scholarly journals Identification of SSR Markers Linked to Regions Associated with Protein Content in F2 Population of Rice (Oryza sativa L.)

Author(s):  
D. Sravani ◽  
C. N. Neeraja ◽  
V. Ravindrababu ◽  
M. Rajendar Reddy

Improvement of rice proteins is important in rice breeding for high nutritional quality. The objective of this study was to identify protein associated regions in the chromosomes in F2 population of the cross derived from swarna and Mahamaya, Parental polymorphism survey between Mahamaya and Swarna was studied using 24 SSR markers. Out of 24 markers, 4 (16%) were polymorphic, 185 F2 plants were assayed individually for protein content and genotyping. Total grain protein content ranged from 4.12 to 12.0 per cent in F2 population. The strategy of selective genotyping was carried out with the F2 plants. The results showed that these three markers RM1369 on chromosome number 6, RM263 on chromosome number 2 and RM337 on chromosome number 8 were unlinked among themselves. Since for mapping a minimum of two markers per locus are required, the data obtained for this study were analyzed by Recombination frequency. Recombination frequency values revealed the association of markers, RM1369 (Chromosome-6) with high protein content (Recombination frequency=0.41) and low protein content (Recombination frequency=0.25), RM263(Chromosome-2) with high protein content (Recombination frequency =0.37%) and low protein content (Recombination frequency =0.33%) and RM337 (Chromosome-8) with high protein content (Recombination frequency =0.22%) and low protein content (Recombination frequency =0.18). This trial study reveals that the above markers can be utilized for identification of regions associated with protein content in rice.

Author(s):  
V. V. Liubych ◽  

Purpose. To investigate the formation of cereal properties of spelt wheat grain depending on variety and line. Methods. Laboratory, mathematical and statistical, physicochemical. Results. As a result of studies it is found that technological properties of grain significantly vary depending on spelt variety and weather conditions. The protein content in spelt grain ranges from 11.0 to 21.3 % depending on the variety. A very high protein content in spelt grain (over 18.0 %) is in variety Zoria of Ukraine; rather high protein content (16.0–17.9 %) is in varieties Schwabenkorn (17.6 %), NSS 6/01 (17.3 %), Avstraliiska 1 (16.7 %) and LPP 3218 (16.7 %); low protein content (12.0–13.9 %) is in varieties LPP 3435 (13.1 %)and LPP 1224 (13.0 %); very low protein content is in varieties Shvedska 1 (11.0 %) and LPP 3117 (11.5 %); the rest of varieties has this indicator at the level of average – 14.0–15.9 %. Vitreous consistency of the endosperm has corn of varieties Zoria of Ukraine and Avstraliiska 1, semi vitreous consistency has grain of varieties NSS 6/01, Schwabenkorn, Frankenkorn and lines LPP 3218, LPP 3132, LPP 1305, LPP 1197, LPP 3124, LPP 3435, semi floury consistency has Shvedska 1 and LPP 3117. Culinary assessment of rolled spelt grains varies significantly depending on the variety. Total culinary assessment of rolled grain porridge of the spelt ranges from 6,8 to 9,0 points. Origin of spelt variety does not affect this indicator. The highest culinary assessment (8.9–9.0 points) has porridge obtained from grain of the variety Zoria of Ukraine and LPP 3132 line which makes it possible to use its grain to obtain cereal products. The lowest assessment of the porridge has variety Shvedska 1 and lines LPP 1224, LPP 3117 – 6.8–7.7 points. The other varieties have a culinary assessment of the porridge at a level of 8.1–8.8 points. For spelt grain indicator of protein content and grain vitreuesness can be used to evaluate the culinary properties of cereals. Conclusions. As a result of the conducted researches, it is established that Zoria Ukrainy, Schwabenkorn, NSS 6/01, Australian 1 varieties and LPP 3218 line are characterized by high protein content. Hard consistency of endosperm has the grain of Australian 1 and Zoria Ukrainy varieties in which the grain hardness is 73 and 84%, respectively. The origin of spelt wheat variety does not affect the color of cooked porridge. The general culinary evaluation of rolled spelt wheat porridge ranges from 6.8 to 9.0 points. Zoria Ukrainy spelt grain has the highest culinary evaluation which makes it possible to use it to produce cereals. To evaluate the culinary properties of spelt cereals, grain protein content and its hardness can be used.


1938 ◽  
Vol 16c (1) ◽  
pp. 1-15 ◽  
Author(s):  
K. W. Neatby ◽  
A. G. McCalla

High yielding varieties of wheat and barley have a marked tendency to be constitutionally low in protein content. While varieties characterized by moderately high yield and high protein content are known, it is doubtful whether the maximum possible yield can be combined with maximum possible protein. The problem of breeding hard red spring wheats is complicated by this relation, while breeding soft wheats and low protein malting barleys is simplified.


Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1186
Author(s):  
Fidel Toldrá ◽  
Leticia Mora

Foods and their industry by-products constitute very good sources of bioactive peptides, which can be naturally generated during processing but are also extensively produced through enzymatic hydrolysis, microbial fermentation, and even during gastrointestinal digestion in the human body [...]


Revista CERES ◽  
2017 ◽  
Vol 64 (2) ◽  
pp. 159-166 ◽  
Author(s):  
Helton Santos Pereira ◽  
Renata Cristina Alvares ◽  
Leonardo Cunha Melo ◽  
Antônio Félix da Costa ◽  
Hélio Wilson Lemos de Carvalho

ABSTRACT The objectives of this work were to study the genetic variability and the interaction between genotypes and environments for cooking time and protein content of bean grains as well as to identify elite lines of Carioca grain type with short cooking time, high protein content and high adaptability and stability for these two traits. Sixteen experiments were conducted in a complete randomized block design with three replications during the rainy, dry and winter seasons, in Goiás, Distrito Federal, Pernambuco, Sergipe, Bahia and Paraná States, in 2009 and 2010. Each trial was composed by 16 elite lines of Carioca grain type and the data of cooking time and protein content were obtained. Data were submitted to analysis of variance and to stability and adaptability analysis, according to the methodology proposed by Annichiarico. Genetic variability was found for cooking time and for protein content among Carioca common bean elite lines; however, for protein content this variability is lower. The environmental effect is important for the expression of these traits and is larger than the genetic effect. The interaction between genotypes and environments is important for cooking time and for protein content of common beans. The lines CNFC 11951 and CNFC 11962 presents short cooking time, high protein content and high stability and adaptability for both traits.


2021 ◽  
Vol 5 (2) ◽  
pp. 108
Author(s):  
D Yadi Heryadi ◽  
Ristina Siti Sundari ◽  
Rini Agustini ◽  
Andang Hidayat

The agribusiness of oyster mushrooms (Pleurotus ostreatus) is getting interested due to an increase in demand. It has almost complete nutrients, high protein content that can substitute animal protein for vegetarians. The growth medium for oyster mushrooms commonly uses sawdust. The cocopeat waste medium is an innovation. The research descriptively compares production cost, yield, revenue, income, and feasibility throughout both mediums. Findings that the total cost of sawdust medium was higher 750,000IDR than cocopeat waste. In terms of income, sawdust medium earned 140.72%, while cocopeat waste earned 133.29%. The feasibility of sawdust waste was 2.41 while cocopeat waste was 2.33 as well. Otherwise, the cocopeat waste medium was higher 99.21IDR than sawdust waste in cost/kg yield. Net income using cocopeat waste medium was 2,739,694.50IDR, and cocopeat waste was 2,511,769.50IDR on average. Both cocopeat waste and sawdust waste medium are very feasible to be an agribusiness. The consideration is that the availability surrounds the production site, and cocopeat waste is cheaper than sawdust waste. Agribisnis jamur tiram (Pleurotus ostreatus) semakin diminati oleh karena permintaan terus meningkat. Kandungan gizinya lengkap dengan protein yang tinggi. Bahkan bagi vegetarian menjadi pengganti protein hewani. Media pertumbuhan jamur tiram biasanya menggunakan limbah serbuk gergaji. Media limbah serbuk sabut kelapa merupakan inovasi baru. Penelitian ini ingin mengetahui biaya-biaya hingga kelayakan usaha agribisnis jamur tiram menggunakan media limbah serbuk sabut kelapa dan yang biasa yaitu limbah serbuk gergaji Ternyata, Biaya total setahun menggunakan media limbah serbuk sabut kelapa lebih hemat Rp750.000 dibandingkan dengan media limbah serbuk gergaji. Biaya per kilogramnya sedikit lebih mahal Rp99.21 tetapi hasil panen lebih rendah dari limbah serbuk gergaji. Jamur tiram media limbah serbuk sabut kelapa memberikan keuntungan 133.29% dan media limbah serbuk gergaji 140.72%. Jamur tiram media limbah serbuk gergaji memberikan keuntungan bersih/bulan rata-rata Rp2,739,694.50, sedangkan media limbah serbuk sabut kelapa rata-rata Rp2,511,769.50.Kedua media untuk jamur tiram sangat layak diusahakan dengan R/C ratio 2.33 dan 2.41. Pengusaha bisa mempertimbangkan media limbah serbuk sabut kelapa atau limbah serbuk gergaji tergantung ketersediaan di sekitar lokasi dengan informasi harga limbah sabut kelapa lebih murah daripada limbah serbuk gergaji.


Author(s):  
В. І. Козечко

Результати проведених експериментальних дослі-джень свідчать, що за вирощування різних сортівпшениці озимої після ріпаку ярого в умовах північногоСтепу України одержання найбільш якісного зерна(третього класу якості) забезпечує сівба 25 вереснята 5 жовтня. Сівба 5 та 15 вересня забезпечувалаодержання зерна, здебільшого, п’ятого й четвертогокласів якості. Із-поміж досліджуваних сортів пше-ниці озимої найбільшим вмістом білку (11,2–13,0 %) іклейковини (17,5–22,7 %) у зерні вирізнявся сорт Се-лянка. Найменшими ці показники були у сорту Подо-лянка – 10,6–12,5 та 17,1–21,9 % відповідно. Об¢ємхліба, отриманого з борошна пшениці озимої сортуСелянка, був найвищим. Так, у середньому за 2008–2010 рр. він коливався від 495 см3 за сівби 5 вереснянормою висіву 4 млн схожих насінин/га до 612 см3 засівби 5 жовтня цією ж нормою. Встановлено впливнорм висіву насіння на показники якості зерна. Так,найвищий вміст білку і клейковини в зерні, а такожоб’єм хліба відмічено на варіантах досліду, де сівбупроводили нормою 4 млн схожих насінин/га. Збіль-шення норми висіву призводило до зменшення данихпоказників. У середньому за роки досліджень найвищуврожайність формував сорт Селянка (4,89 т/га) засівби 25 вересня нормою 5 млн схожих насінин/га.Максимальну врожайність рослини сорту Золотоко-лоса – 4,20 та 4,19 т/га – формували, відповідно, засівби 5 жовтня нормою 6 млн схожих насінин/га та 25вересня нормою висіву 5 млн схожих насінин/га. Най-нижчу серед сортів урожайність формував сортПодолянка, що коливалася за період проведення до-сліджень у межах 2,99–3,75 т/га. Results of the conducted pilot researches testify that at cultivation of various sorts of wheat winter after a spring rape in the conditions of the northern Steppe of Ukraine, receiving the most qualitative grain, the third class of quality, provides sowing on 25 September and on 5 October. Sowing on 5 and 15 September provided grain, generally the fifth and fourth classes of quality. Among sorts of wheat winter which were studied in the experiences, the greatest protein content (11,2–13,0%) and glutens (17,5–22,7%) in grain Selyank's sort differed. The smallest Podolyank's sort of – 10,6–12,5 and 17,1–21,9% had these indicators respectively. The volume of the bread received from a flour of wheat of winter of a sort of Selyanka, was the highest. So, on the average for 2008–2010 it fluctuated from 495 cm3 when sowing on 5 September seeding rate of 4 million units germinating seeds/ha to 612 cm3 when sowing on 5 October the same norm. Influence of seeding rate on indicators of quality of grain is established. So, high protein content and glutens in grain, and also the volume of bread are noted on experience options where sowing carried out norm of 4 million units germinating seeds/ha. The increase in seeding rate led to reduction of these indicators. Average, over years of research, the highest yield sort Selyanka formed (4,89 t per ha) when sowing on 25 September norm of 5 million units germinating seeds/ha. The maximum productivity of a plant of a grade of Zolotokolosa – 4,20 and 4,19 t per ha formed, respectively, when sowing on 5 October norm of 6 million units germinating seeds/ha and on 25 September seeding rate of 5 million units germinating seeds/ha. The low among grades productivity was formed by a sort of Podolyanka at which it fluctuated, during carrying out researches, within 2,99–3,75 t per ha.


2021 ◽  
pp. 407
Author(s):  
Wibawa Prasetya ◽  
Yanto Yanto ◽  
Christine Natalia ◽  
Agustinus Silalahi

This time, rubbish is a very serious environmental problem for the people of Kampung Baru, Sampora Village, Tangerang. The increase in population results in an increase in the amount of rubbish. According to data, rubbish production in Tangerang in 2020 reached 22,873 tons, while the rubbish handled was only around 937 tons (4%).This problem needs to be solved immediately. Rubbish generated by households can be in the form of organic or inorganic. If rubbish is not managed properly, it can damage the surrounding environment.The method   used to solve the problem of organic rubbish can be done through bioconversion of organic rubbish into maggot.Organic rubbish is often considered safe because it is easily biodegradable, even though organic rubbish has the potential to damage the environment, This method has advantages, because during the bioconversion process it does not cause unpleasant odors Bioconversion of organic rubbish can be a rubbish management solution that can be offered to the residents of Kampung Baru..Every 1 kg of maggot requires 2 kg of organic rubbish/hour as food. The speed of maggot using organic rubbish  as feed can be an alternative to reduce the amount of organic rubbish.Maggot that is ready to be harvested has a very high protein content, so it can be used as feed for catfish or  poultry.Catfish fed with maggot as feed, at the age of 100 days, each kilogram contained 6 catfish, while catfish fed only with pellets, at the age of 100 days, each kg contained 7-8 catfish. Thus maggot can increase the weight of catfish significantly.In addition, maggot can reduce pellet consumption by up to 75 percent, so it can help catfish farmers in saving feed costs.Saat ini sampah merupakan masalah lingkungan yang sangat serius bagi masyarakat Kampung Baru, Desa Sampora, Tangerang. Pertambahan jumlah penduduk mengakibatkan pertambahan jumlah sampah. Menurut data produksi sampah di Tangerang pada tahun 2020 mencapai 22.873 ton, sedangkan sampah yang tertangani hanya sekitar 937 ton (4%). Permasalahan ini perlu segera dicari solusinya. Sampah  yang dihasilkan oleh rumah tangga dapat berupa sampah organik maupun anorganik. Jika sampah tidak dikelola dengan baik, maka sampah dapat merusak lingkungan sekitar. Sampah organik sering dianggap aman karena mudah terurai, padahal  sampah organik memiliki potensi untuk merusak lingkungan.Metode yang digunakan untuk memecahkan permasalahan sampah organik dapat dilakukan melalui biokonversi sampah organik menjadi maggot. Metode ini mempunyai kelebihan, karena selama proses biokonversi tidak menimbulkan bauyang tidak sedap. Biokonversi sampah organik dapat menjadi  solusi pengolahan sampah yang dapat  ditawarkan kepada penduduk Kampung Baru, Setiap 1 kg maggot membutuhkan 2 kg sampah organik/jam sebagai makanannya. Kecepatan maggot menggunakan sampah organik sebagai pakan, dapat menjadi alternatif untuk mereduksi jumlah sampah organik.  Maggot yang siap dipanen mempunyai kandungan protein yang sangat tinggi, sehingga dapat digunakan sebagai pakan lele atau ternak unggas lainnya. Lele  yang diberi maggot sebagai pakan, pada usia 100 hari, setiap kilogram berisi 6 ekor lele, sedangkan lele yang hanya diberi pakan pelet, pada usia 100 hari,setiap kg berisi 7-8 ekor lele. Dengan demikian maggot dapat meningkatkan berat lele secara signifikan. Selain itu maggot dapat mengurangi konsumsi pelet sampai 75 persen, sehingga dapat membantu peternak lele dalam menghemat biaya pakan.


Heliyon ◽  
2019 ◽  
Vol 5 (4) ◽  
pp. e01508
Author(s):  
Ewan Thomas ◽  
Bettina Karsten ◽  
Fatma Nese Sahin ◽  
Goktug Ertetik ◽  
Francesco Martines ◽  
...  

1988 ◽  
Vol 75 (2) ◽  
pp. 151-157 ◽  
Author(s):  
L. R. Solomon ◽  
J. C. Atherton ◽  
H. Bobinski ◽  
S. L. Cottam ◽  
C. Gray ◽  
...  

1. The effect of meals with a high and low protein content and of the fasting state on renal function and plasma atrial natriuretic peptide was studied in water-loaded normal volunteers. 2. Creatinine clearance increased after the high protein meal, but did not change after the low protein meal or while fasting. Observations of similar increases in urine sodium and potassium excretion and a transient decrease in urine flow after both meals suggest that the protein content of the meal is not an important contributory factor in these responses to feeding. 3. Absolute delivery of sodium and water out of the proximal tubules (assessed by the lithium clearance method) was higher after both meals than while fasting; fractional lithium clearance was higher after the low protein meal than the high protein meal and while fasting. Absolute reabsorption from proximal tubules was increased after only the high protein meal. 4. A transient decrease in the fraction of water delivered to distal nephron segments that appeared in the urine (fractional distal water excretion) was observed after both meals. Fractional distal sodium excretion and absolute distal sodium and water reabsorption increased after both meals. 5. Since plasma atrial natriuretic peptide either decreased (high protein meal) or remained unchanged (low protein meal and fasting), it is unlikely that this hormone is involved in the hyperfiltration after the high protein meal and the natriuresis after both high and low protein meals.


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