scholarly journals Mangifera indica (Mango) Bark Therapy Potentiates Wound Healing in Diabetic Rats

2020 ◽  
pp. 1-13
Author(s):  
Rotimi Sunday Ajani ◽  
Opeyemi Jeremiah Olateju
Keyword(s):  
Wound Healing ◽  
Mangifera Indica ◽  
Foot Ulcer ◽  
Ethanolic Extract ◽  
Diabetic Rats ◽  
Wound Contraction ◽  
Female Wistar Rats ◽  
Microscopic Features ◽  
Cultivated Plant ◽  
The Mean

Objective: One of the debilitating complications of diabetes mellitus is chronic foot ulcer. Mangifera indica (Mango) is a naturally occurring and widely cultivated plant with many health benefits attributable to its parts. The wound healing potential of its bark in adult diabetic rats was investigated. Methods: The excised wounds of diabetic adult female wistar rats in groups of six were dressed with mango bark powder (MPD), mango bark ethanolic extract (MED), sofratulle (SD) and normal saline (ND) daily till healed. The corresponding control groups were MPC, MEC, SC and NC respectively. Every three days, the mean wound contraction rates were calculated from the measured wound areas. Granulation tissue was biopsied from an animal per group on day 3, 6 and 9 for histopathological evaluation and after healing, the scars of the remaining animals were biopsied for histology. Results: The M. indica bark powder contained greater quantity of coumarins than the ethanolic extract; with terpenoids and steroids detected only in the powder. The MPD group had the highest mean wound contraction rates for the specified period. The mean wound contraction rates for the MPC group were significantly higher than those of the MED. The granulation tissues of the MPD and MPC groups had similar microscopic features to those of MEC, MED, SC, SD, NC and ND. Microscopy of the wound scars showed stratified squamous epithelia with abundant collagen fibres and blood vessels with dermal appendages seen in some of the groups. Conclusion: Findings from this study showed that the M. indica bark.

scholarly journals Nicotiana tabacum (Tobacco) Promotes Wound Healing in Diabetic Rats

2020 ◽  
pp. 31-45
Author(s):  
Rotimi Sunday Ajani ◽  
Oluwafisayomi Tesleem Balogun
Keyword(s):  
Wound Healing ◽  
Nicotiana Tabacum ◽  
Ethanolic Extract ◽  
Diabetic Rats ◽  
Wound Contraction ◽  
Control Group ◽  
Contraction Rate ◽  
Male Wistar Rats ◽  
The Mean ◽  
Tobacco Extract

Objective: The health hazards of tobacco smoking and diabetes mellitus constitute major and diverse global burden. The influence of Nicotiana tabacum (tobacco) powder and ethanolic extract on excised wound of diabetic rats was investigated. Methods: The wounds of diabetic adult male wistar rats in groups of six were dressed with tobacco powder (TPD), tobacco ethanolic extract (TED), sofratulle (SD) and normal saline (ND) daily till healed. There were composite control groups namely TPC, TEC, SC and NC. Every three days, the wound areas were measured in order to calculate the mean wound contraction rates. Granulation tissue was biopsied from an animal per group on day 3,6 and 9 for histopathological evaluation and after healing, the scars of the remaining animals were biopsied for histology. Results: On day 3, the tobacco powder diabetic (TPD) group had the highest mean wound contraction rate and even higher than its control group. At day 18, the tobacco extract control group had the least mean wound contraction rate. The mean wound contraction rates of some diabetic groups were significantly higher than those of the respective control on day 3 and 12 (TPD vs TPC; TED vs TEC). The TPD mean wound contraction rates were significantly higher than those of ND on day 3,6,9 and 12. Histology of the granulation tissues of the tobacco diabetic groups was similar to those of the other groups. Sections of the wound scars revealed stratified squamous epithelia with abundant collagen fibres and blood vessels in all the groups. It was also observed that the scars were more fibrous than cellular with dermal appendages observed in some of the groups. Conclusion: Topical application of Nicotiana tabacum (Tobacco) promotes wound healing with scars comparable to those of rats dressed with sofratulle.

scholarly journals Unripe Rind and Pulp of Ananas comosus Accelerate Wound Healing in Diabetic Wistar Rat

2019 ◽  
pp. 1-13
Author(s):  
Rotimi Sunday Ajani ◽  
Nurudeen Abiola Opadokun
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Foot Ulcer ◽  
Wistar Rat ◽  
Wound Contraction ◽  
Ananas Comosus ◽  
Control Group ◽  
Accelerate Wound Healing ◽  
The Mean ◽  
Diabetic Wounds

Objective: One of the sequelae of diabetes mellitus is chronic foot ulcer. This study investigated wound healing propensity of the pulp and rind of Ananas comosus (a widely cultivated fruit with many health benefits) in diabetic wistar rats. Methods: The excisional wounds of twenty four adult wistar rats with induced diabetes in four groups of six each were dressed with the pulp powder (PPD), pulp extract (PED), rind powder (RPD) and rind extract (RED) of A. comosus daily till healed. Also the mean wound contraction rates were calculated from the wound areas every three days. Granulation tissue was biopsied from an animal per group on day 3, 6 and 9 for histopathological evaluation. Each of these groups had a corresponding equal number control group with similar procedures performed. Results: The mean wound contraction rates of the rind pulp diabetic (RPD) and rind extract diabetic (RED) groups paralleled those of their respective control group (RPC, REC) as they were not significantly different. Also amongst the diabetic groups, the rind of A. comosus demonstrated greater wound healing capability over the respective pulp counterpart (PPD, PED) as evidenced by significantly higher mean wound contraction rates. Histopathologic features of the granulation tissues and scars of the diabetic groups were similar to those of the control groups. Conclusion: Although, both the rind and pulp of unripe A. comosus either in extract or powder formulation were shown in this study to possess excellent healing potentials in diabetic wounds, the rind appeared to be better off and may be a viable alternative to the wound dressing materials currently in use in the management of diabetic wounds / ulcers.

scholarly journals Experimental Evaluation of Ethanolic Extract ofCarapa guianensisL. Leaf for Its Wound Healing Activity Using Three Wound Models

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
B. Shivananda Nayak ◽  
Joel Kanhai ◽  
David Malcolm Milne ◽  
Lexley Pinto Pereira ◽  
William H. Swanston
Keyword(s):  
Wound Healing ◽  
Drinking Water ◽  
Granulation Tissue ◽  
Dead Space ◽  
Breaking Strength ◽  
Test Group ◽  
Ethanolic Extract ◽  
Wound Contraction ◽  
Hydroxyproline Content ◽  
Wound Healing Activity

The leaves ofCarapa guianensishave been used to treat ulcers, skin parasites, and skin problems. The ethanolic extract ofC. guianensisleaf was evaluated for its antibacterial and wound healing activity using excision, incision and dead space wound models in rats. The animals were randomly divided into two groups (n= 6) in all the models. In the excision wound model test group animals were treated topically with the leaf extract (250 mg kg−1body weight) whereas, control animals were treated with petroleum jelly. In the incision and dead space wound models, the test group animals were treated with extract (250 mg kg−1day−1) orally by mixing in drinking water and the control group animals were maintained with plain drinking water. Healing was assessed by the rate of wound contraction, period of epithelialization, skin breaking strength, granulation tissue weight and hydoxyproline content. On Day 15 extract-treated animals exhibited 100% reduction in the wound area when compared to controls (95%) with significant decrease in the epithelialization period. The extract failed to demonstrate antibacterial activity. Skin breaking strength (P< .001), wet (P< .002) and dry (P< .02) granulation tissue and hydroxyproline content (P< .03) were significantly higher in extract treated animals. The increased rate of wound contraction, skin breaking strength and hydroxyproline content supports potential application ofC. guianensisin wound healing.

scholarly journals Evaluation of Wound Healing Activity of an Ethanolic Extract of Anacardiumoccidentale Leaves in Wistar Rats

2020 ◽  
Vol 13 (4) ◽  
pp. 2061-2068
Author(s):  
Meena Kumari K ◽  
Amberkar Mohanbabu Vittalrao ◽  
Praveen Kumar SE ◽  
Sushma Prabhath ◽  
Charitha Charitha
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Histopathological Examination ◽  
Ethanolic Extract ◽  
Wound Contraction ◽  
Saline Control ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Healing Activity

BACKGROUND -This study undertaken to screen a potential wound healing activity of an ethanolic extract of Anacardiumoccidentale(Ao)leaves in Wistar rats. MATERIALS AND METHODS-Excision wound of 500mm2created on the back of Wistar rats. A total of thirty adult Wistar rats were prescreened and divided into five groups of six rats each.Group I and II rats were treated by normal saline (control) and povidone-iodine (standard drug), respectively. Group III and IV with 0.5% and 2%of Anacardiumoccidentale ointment (test drugs), respectively, and Group V rats treated with anti-wound healer drug dexamethasone, i.m. + 2% Anacardiumoccidentale. All drugs were applied topically except Inj. dexamethasone. In the model of excision wound, the wound healing effect was determined by parameters such as the percentage of wound contraction, duration of epithelialization, and histopathological examination. RESULT-All drugs, povidone-iodine, 0.5%, and 2% of the Ao ointment treated group of rats showed a statistically significant increase (P<0.05) in the % of wound contraction on 5th and 6thdays Vs.Control rats. In contrast,povidone-iodine, 2% Ao ointment and (dexamethasone + 2% Ao)treated rats decreased the period of epithelialization which was statistically significant (P<0.05) compared to the control.However, the standard povidone-iodine treated rats showed a maximum increase in the percentage of wound contraction and minimum period of epithelialization, followed by a higher dose of 2% Ao extract. The plant extract 0.5% and 2% of Ao showeda significant increase inthe rate of wound contraction as compared to the standard drug povidone-iodine also. The combination of (2%Aoextract+dexamethasone) drug-treated rats decreased the rate of wound contraction on 5th, 9th days; whereas, povidone-iodine increased on all days, i.e., 5th, 9th, 13th and 17th days,which was statistically significant (p<0.05) Vs.Normal saline. The povidone and 2% of Ao extract hastened the rate of wound healing activity, which was evident by maximum re-epithelialization and presence of the collagen fibers. CONCLUSION-In our study, the topical application of Anacardiumoccidentale leaf extract has shown promising wound healing activity; however, it failed to reverse the anti-wound healing property of steroids.

WOUND HEALING ACTIVITY OF TRIPHALA IN EXPERIMENTALLY INDUCED DIABETIC RATS

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (04) ◽  
pp. 70-75
Author(s):  
M. P. Singh ◽  
S Vashisht ◽  
V. Chawla ◽  
P. Kumari ◽  
Keyword(s):  
Wound Healing ◽  
Oral Administration ◽  
Dead Space ◽  
Diabetic Rats ◽  
Wound Contraction ◽  
Control Group ◽  
Excision Wound ◽  
Wound Model ◽  
Diabetic Wound Healing ◽  
Healing Property

Aim of the present study was to explore the wound healing potential of Triphala extract. Triphala ointment (15% w/w) was used in the study for excision wound model. For dead space wound model Triphala extract (200 mg/kg) was administered orally. Extract of Triphala topically applied in excision wound model was found to possess wound healing property. On day 11th, the extract treated animals showed wound contraction to the extent of 87.63% and 94.77%, compared with control group of animals. In dead space wound model, oral administration of the extract was found to increase the wet and dry granuloma weight of tissue. The extract treated animals showed high quantity of hydroxyproline content when compared with the control group animals. The topical and oral administration of Triphala extract promotes diabetic wound healing in streptozotocin induced diabetic rats with increased rate of wound contraction and collagen turnover.

Evaluation of Effect of Biologically Synthesized Ethanolic Extract of Propolis-Loaded Poly(-Lactic-co-Glycolic Acid) Nanoparticles on Wound Healing in Diabetic Rats

2022 ◽  
pp. 153473462110732
Author(s):  
Reza Tayfeh-Ebrahimi ◽  
Amir Amniattalab ◽  
Rahim Mohammadi
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Granulation Tissue ◽  
Glycolic Acid ◽  
Ethanolic Extract ◽  
Plga Nanoparticles ◽  
Diabetic Rats ◽  
Diabetic Wound ◽  
Wound Size ◽  
Ethanolic Extract Of Propolis

Wound healing is interaction of a complex cascade of cellular/biochemical actions leading to restoration of structural and functional integrity with regain of injured tissues strength. This study was aimed at evaluation of application of ethanolic extract of propolis-loaded poly(-lactic-co-glycolic acid) nanoparticles (EEP-PLGA NPs) on wound healing in diabetic rats. Sixty rats were randomized into four groups of 15 rats each: In control group (Control) diabetic wound was treated with normal saline. In Carrier 1 group diabetic wound was treated with PLGA nanoparticles based solution. In Carrier 2 group the diabetic wound was treated with EEP. In Treatment group animals received EEP-PLGA NPs on the wound. Wound size was measured on 7, 14 and 21 days after surgery. The expression of p53, bcl-2, Caspase III, were evaluated using reverse-transcription PCR and Immunohistochemical staining. The Treatment group had significantly reduced the wound size compared to other groups ( P = 0.001). histological and morphometric studies, and mean rank of the qualitative studies demonstrated that there was significant difference between Treatment group and other groups ( P < .05). Observations demonstrated that ethanolic extract of propolis-loaded PLGA nanoparticles significantly shortened the inflammatory phase and accelerated the cellular proliferation. Accordingly, the animals in Treatment group revealed significantly ( P < .05) higher fibroblast distribution/one mm2 of wound area and rapid re epithelialization. The mRNA levels of bcl-2, p53 and caspase III were remarkably ( P < .05) higher in Treatment group compared to control and animals. The immunohistochemical analyzes confirmed the RT-PCR findings. EEP-PLGA NPs offered potential advantages in wound healing acceleration and improvement through angiogenesis stimulation, fibroblast proliferation and granulation tissue formation in early days of healing phases, acceleration in diabetic wound repair associated with earlier wound contraction and stability of damaged area by rearrangement of granulation tissue and collagen fibers.

scholarly journals Wound Healing Properties of Ethanolic Extract of Acrostichum aureum and Acrostichum speciosum Rhizome in Rats

2013 ◽  
Vol 1 (2) ◽  
pp. 42-48
Author(s):  
Hendy Putra Herman ◽  
Deny Susanti ◽  
Shahbudin Saad ◽  
Muhammad Taher ◽  
Norazsida Ramli
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Wound Contraction ◽  
Wound Healing Process ◽  
Beneficial Effects ◽  
Biopsy Punch ◽  
Excisional Wound ◽  
Negative Controls

This paper presents the assessment of wound healing properties of ethanolic extract of Acrostichum aureum and A. speciosum in rats. It was carried out on 6 treated groups with 6 rats each group. The excisional wound was made on the dorsal interscapular region of each rat by a 6 mm biopsy punch. T1 and T2 were treated with 5% and 10% A. aureum ethanolic extract in aqua cream, respectively. Meanwhile, T3 and T4 were treated with 5% and 10% A. speciosum ethanolic extract in aqua cream, respectively. Solcoseryl jelly and aqua cream were used as positive and negative controls. The treatments were applied topically twice daily, wound contraction and period of epithelization were measured every 2 days. The results showed that wound treated with 10% A. speciosum (T4) exerted faster wound contraction significantly (p < 0.05) compared to the negative control. It was also enhanced epithelization period (9.33 ± 3.20 days) of the wounds significantly (p < 0.05). In conclusion, these results strongly suggested the beneficial effects of both plant extracts for enhancing wound healing process.

scholarly journals Therapeutic and Ameliorative Effects of Active Compounds of Combretum molle in the Treatment and Relief from Wounds in a Diabetes Mellitus Experimental Model

Coatings ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 324
Author(s):  
Reham Z. Hamza ◽  
Shaden E. Al-Motaani ◽  
Tarek Al-Talhi
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Diabetic Foot ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Diabetic Rats ◽  
Male Rats ◽  
Foot Ulcers ◽  
Control Group ◽  
Combretum Molle

Foot ulcers are one of the leading causes of severe and high mortality in diabetics. It is known that wound healing in diabetics is a very complicated process due to the direct severe effect of diabetes mellitus on blood vessels, causing difficulty in wound healing. Many methods of treatment have recently been employed for novel dressings for the promotion of tissue regeneration and rapid wound closure. Combretum molle is composed of chemical compounds, such as lignin, gallic acid, and ellagic acid. Twenty male rats that were 4 months of age were divided into a I-a diabetic foot ulcer group as the control group and a II-a diabetic group (wound + Combretum molle). This study investigated the antioxidant and excellent healing effects of the extract of Combretum molle in repairing skin damaged by diabetes. This was confirmed by elevated antioxidant enzymes in the animals’ tissues in diabetic rats treated with this extract. The recovery of the alterations in the skin layers led to great improvements, which proved the Combretum molle’s promising capacity for wound healing in diabetics with wounds that mimic diabetic foot ulcers. This treatment can be considered a promising opportunity for patients with severe foot ulcers and may open the door to more discoveries about this plant’s medical capabilities.

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