scholarly journals Unripe Rind and Pulp of Ananas comosus Accelerate Wound Healing in Diabetic Wistar Rat

2019 ◽  
pp. 1-13
Author(s):  
Rotimi Sunday Ajani ◽  
Nurudeen Abiola Opadokun
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Foot Ulcer ◽  
Wistar Rat ◽  
Wound Contraction ◽  
Ananas Comosus ◽  
Control Group ◽  
Accelerate Wound Healing ◽  
The Mean ◽  
Diabetic Wounds

Objective: One of the sequelae of diabetes mellitus is chronic foot ulcer. This study investigated wound healing propensity of the pulp and rind of Ananas comosus (a widely cultivated fruit with many health benefits) in diabetic wistar rats. Methods: The excisional wounds of twenty four adult wistar rats with induced diabetes in four groups of six each were dressed with the pulp powder (PPD), pulp extract (PED), rind powder (RPD) and rind extract (RED) of A. comosus daily till healed. Also the mean wound contraction rates were calculated from the wound areas every three days. Granulation tissue was biopsied from an animal per group on day 3, 6 and 9 for histopathological evaluation. Each of these groups had a corresponding equal number control group with similar procedures performed. Results: The mean wound contraction rates of the rind pulp diabetic (RPD) and rind extract diabetic (RED) groups paralleled those of their respective control group (RPC, REC) as they were not significantly different. Also amongst the diabetic groups, the rind of A. comosus demonstrated greater wound healing capability over the respective pulp counterpart (PPD, PED) as evidenced by significantly higher mean wound contraction rates. Histopathologic features of the granulation tissues and scars of the diabetic groups were similar to those of the control groups. Conclusion: Although, both the rind and pulp of unripe A. comosus either in extract or powder formulation were shown in this study to possess excellent healing potentials in diabetic wounds, the rind appeared to be better off and may be a viable alternative to the wound dressing materials currently in use in the management of diabetic wounds / ulcers.

scholarly journals WOUND HEALING ACTIVITY OF LEMON PEPPER ESSENTIAL OIL IN BURN WOUND OF WISTAR RAT

2021 ◽  
pp. 122-125
Author(s):  
DEWI PURNAMA ◽  
CHRISMIS NOVALINDA GINTING ◽  
LINDA CHIUMAN ◽  
ADRIAN KHU
Keyword(s):  
Wound Healing ◽  
Essential Oil ◽  
Wistar Rat ◽  
Wound Contraction ◽  
Initial Time ◽  
Control Group ◽  
Burn Wound ◽  
Accelerate Wound Healing ◽  
Significant Difference ◽  
Wound Healing Activity

Objective: The purpose of the study was to investigate the acceleration of the wound healing properties from lemon pepper’s essential oil as the lemon pepper’s ointment. Methods: There were 20 Wistar rats as an animal trial divided into four sample groups, including control (ointment base), standard (Nebacetin®), 5% lemon pepper, and 10% lemon pepper ointment, and all groups were injured by electric soldier for 10 s. The wound contraction and epithelialization period were the parameters of wound healing activity. Result: Wound contraction as the parameter of wound healing showed significant difference between the standard and lemon pepper ointment (p<0.05). The lemon pepper ointment groups showed no significant wound contraction difference in each lemon pepper ointment concentration at the initial time of observation, however, it become more obvious at last period of observation. Furthermore, the epithelialization period did not showed any significant differences between standard, 5% or 10% lemon pepper ointment against the control group (p>0.05). Conclusion: It can be concluded that the lemon pepper ointment had the potential to accelerate wound healing activity.

scholarly journals EFEKTIVITAS PEMBERIAN EKSTRAK IKAN HARUAN (Channa striata) TERHADAP JUMLAH NEUTROFIL PADA PROSES PENYEMBUHAN ULKUS TRAUMATIKUS

DENTA ◽  
2019 ◽  
Vol 12 (2) ◽  
pp. 8
Author(s):  
Isidora Karsini ◽  
Farhana Nur Fadhila ◽  
Nafiah Nafiah
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Unsaturated Fat ◽  
Post Test ◽  
Channa Striata ◽  
The Mean

<p><strong><em>Background:</em></strong><em> </em><em>Channa striata contains important compounds in the process of tissue synthesis and important role in wound healing, such as albumin, zinc (Zn), copper (Cu), iron (Fe) and unsaturated fat acids. <strong>Purpose :</strong> To determine the effect of Channa striata extract to the amount of neutrophil in healing process of traumatic ulcer. <strong>Material and Method :</strong> The design of this study used randomized post test only control group design. 18 wistar rats were divided into 6 groups, i.e: K-1 (no treatment for 1 day), K-3 (no treatment for 3 days), K+1 (giving hyaluronic acid 0.2% for 1 day), K+3 (giving hyaluronic acis 0.2% for 3 days), P1 (giving Channa striata extract 100% for 1 day), P3 (giving Channa striata extract 100% for 3 days). <strong>Results :</strong> There was a decrease in the number of neutrophil in the treatment group. The mean and standart deviation of the number of neutrophil in the K-1 group (75,96±3,65), K-3 (50,76±2,04), K+1(72,96±2,97),K+3(45,06±2,83),P1(54,20±1,31),P3(32,50±,85). <strong>Conclusion :</strong> The using of Channa striata extract 100% for 3 days are the most effective in decreasing the amount of neutrophil in healing process of traumatic ulcer of Rattus Novergicus Strain Wistar.</em></p>

scholarly journals Phlebotrophic Effect of Graptophyllum Pictum (L.) Griff on Experimental Wistar Hemorrhoids

2019 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Mario Sadar Bernitho Hutagalung
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Wistar Rats ◽  
Controlled Trial ◽  
Anal Verge ◽  
Wistar Rat ◽  
Control Group ◽  
Random Allocation ◽  
Group I ◽  
The Mean

Background : The 1st and 2nd degree hemorrhoids is managed non-operatively with the anti-inflammatory and plebothropic drugs. Graptophyllum pictum extract (GPE) has already been used widely in Indonesia to treat hemorrhoid with good result, however, the mechanism is not supported by the molecular research.Objective : This study is intended to study the phlebothropic effects of GPE by measuring the degree of edema and extravassal leucocytes of experimental wistar hemorrhoid.Methods : Experimental study with Randomized Controlled Trial Post-test only design in male wistar rats, weight around 200 gr, induced for the development of a disease-like condition of hemorrhoids by 6% croton oil on the anus for 3 days. Random allocation was performed to divide the 14 wistar rats in 2 groups. Group I as control got normal saline solution, while group II was treated with GPE 100mg/kgbw, started on day 4th for 5 consecutive days. On 9th day blood was extracted from retroorbital fossa and anus was resected up to 2 cm from anal verge and weighted. The degree of anal edema was measured by rectoanal coefficient, that is rasio between anal weight (miligrams) and body weight (grams).Results : Until the end of study, all wistar rat were still alive. The mean (±SD) of body weight   of control group was 173.84 ­(±13.37) and the treatment group was 171.70  (±13.10), and there was no significant differences (p = 0.833). The mean (±SD) of rectoanal coefficient in the treatment group was 2.46 (±0.41) and it was significantly lower than control group  (3.13 ± 0.85) (p = 0.029). The mean (±SD) of extravassal leukocytes in the treatment group was 900.14(±48.09) and it was significantly lower than the control (1003.28 ± 99.30) (p = 0.042).Conclusions : Graptophyllum pictum extract at doses of 100 mg/Kgbw have phlebotonic effect  in decreased edema and extravassal leukocytes.

scholarly journals Mangifera indica (Mango) Bark Therapy Potentiates Wound Healing in Diabetic Rats

2020 ◽  
pp. 1-13
Author(s):  
Rotimi Sunday Ajani ◽  
Opeyemi Jeremiah Olateju
Keyword(s):  
Wound Healing ◽  
Mangifera Indica ◽  
Foot Ulcer ◽  
Ethanolic Extract ◽  
Diabetic Rats ◽  
Wound Contraction ◽  
Female Wistar Rats ◽  
Microscopic Features ◽  
Cultivated Plant ◽  
The Mean

Objective: One of the debilitating complications of diabetes mellitus is chronic foot ulcer. Mangifera indica (Mango) is a naturally occurring and widely cultivated plant with many health benefits attributable to its parts. The wound healing potential of its bark in adult diabetic rats was investigated. Methods: The excised wounds of diabetic adult female wistar rats in groups of six were dressed with mango bark powder (MPD), mango bark ethanolic extract (MED), sofratulle (SD) and normal saline (ND) daily till healed. The corresponding control groups were MPC, MEC, SC and NC respectively. Every three days, the mean wound contraction rates were calculated from the measured wound areas. Granulation tissue was biopsied from an animal per group on day 3, 6 and 9 for histopathological evaluation and after healing, the scars of the remaining animals were biopsied for histology. Results: The M. indica bark powder contained greater quantity of coumarins than the ethanolic extract; with terpenoids and steroids detected only in the powder. The MPD group had the highest mean wound contraction rates for the specified period. The mean wound contraction rates for the MPC group were significantly higher than those of the MED. The granulation tissues of the MPD and MPC groups had similar microscopic features to those of MEC, MED, SC, SD, NC and ND. Microscopy of the wound scars showed stratified squamous epithelia with abundant collagen fibres and blood vessels with dermal appendages seen in some of the groups. Conclusion: Findings from this study showed that the M. indica bark.

Evaluation of the cutaneous wound healing potential of tamanu oil in wounds induced in rats

2021 ◽  
Vol 30 (Sup9a) ◽  
pp. Vi-Vx
Author(s):  
Sevil S Erdogan ◽  
Tugba F Gur ◽  
Neslihan K Terzi ◽  
Bilal Dogan
Keyword(s):  
Wound Healing ◽  
Centella Asiatica ◽  
Scientific Data ◽  
The Other ◽  
Wound Contraction ◽  
Control Group ◽  
Plant Oil ◽  
Reference Drug ◽  
Collagen Density ◽  
Diabetic Wounds

Aims: Tamanu is a plant oil derived from the fruit and seeds of the Calophyllum inophyllum tree. Although scientific data on tamanu oil are limited, it is recommended worldwide for the treatment of abrasions, burns, diabetic wounds and scars. This study aimed to compare the wound healing efficacy of the topical use of tamanu oil with a reference drug in rats. Methods: Uniform wounds were induced on the dorsum of 21 rats, randomly divided into three groups. The control group received normal saline; the tamanu group received tamanu oil; and the centella group was treated with Centella asiatica. Wound healing was clinically evaluated using wound healing scoring and wound contraction. A biopsy was taken from the wound sites of each rat on days 7, 14 and 21 for histopathological evaluation. Results: Wound contraction was significantly lower in the tamanu group compared with the other groups. On day 7, the intensity of macrophage infiltration and mature granulation tissues were significantly higher in the centella and tamanu groups than in the control group. Fibrosis and collagen density were higher in the tamanu group than the other groups on day 7. Conclusion: In wound healing in rats, tamanu oil accelerated the formation of macrophage-granulation tissues-fibrosis and resulted in less wound contraction.

scholarly journals Nicotiana tabacum (Tobacco) Promotes Wound Healing in Diabetic Rats

2020 ◽  
pp. 31-45
Author(s):  
Rotimi Sunday Ajani ◽  
Oluwafisayomi Tesleem Balogun
Keyword(s):  
Wound Healing ◽  
Nicotiana Tabacum ◽  
Ethanolic Extract ◽  
Diabetic Rats ◽  
Wound Contraction ◽  
Control Group ◽  
Contraction Rate ◽  
Male Wistar Rats ◽  
The Mean ◽  
Tobacco Extract

Objective: The health hazards of tobacco smoking and diabetes mellitus constitute major and diverse global burden. The influence of Nicotiana tabacum (tobacco) powder and ethanolic extract on excised wound of diabetic rats was investigated. Methods: The wounds of diabetic adult male wistar rats in groups of six were dressed with tobacco powder (TPD), tobacco ethanolic extract (TED), sofratulle (SD) and normal saline (ND) daily till healed. There were composite control groups namely TPC, TEC, SC and NC. Every three days, the wound areas were measured in order to calculate the mean wound contraction rates. Granulation tissue was biopsied from an animal per group on day 3,6 and 9 for histopathological evaluation and after healing, the scars of the remaining animals were biopsied for histology. Results: On day 3, the tobacco powder diabetic (TPD) group had the highest mean wound contraction rate and even higher than its control group. At day 18, the tobacco extract control group had the least mean wound contraction rate. The mean wound contraction rates of some diabetic groups were significantly higher than those of the respective control on day 3 and 12 (TPD vs TPC; TED vs TEC). The TPD mean wound contraction rates were significantly higher than those of ND on day 3,6,9 and 12. Histology of the granulation tissues of the tobacco diabetic groups was similar to those of the other groups. Sections of the wound scars revealed stratified squamous epithelia with abundant collagen fibres and blood vessels in all the groups. It was also observed that the scars were more fibrous than cellular with dermal appendages observed in some of the groups. Conclusion: Topical application of Nicotiana tabacum (Tobacco) promotes wound healing with scars comparable to those of rats dressed with sofratulle.

scholarly journals Potential Wound Healing Properties of a Polyherbal Formulation (Amrit oil) in an Experimental Model on Wistar Rats

Biomedicine ◽  
2020 ◽  
Vol 39 (1) ◽  
pp. 40-51
Author(s):  
R Malarvizhi ◽  
Poddar Abhishek ◽  
R Barathidasan ◽  
Kanuga Kishore K. ◽  
HR Vasanthi
Keyword(s):  
Wound Healing ◽  
Experimental Model ◽  
Wound Repair ◽  
Wistar Rats ◽  
Wound Contraction ◽  
Control Group ◽  
Histopathological Analysis ◽  
Standard Drug ◽  
Polyherbal Formulation ◽  
Group I

Introduction and Aim: Wound healing is an integrated process of cellular and biochemical events in restoring the structural functionality of the damaged tissue caused due to wounds. In the present study, the wound healing potential of an ayurvedic polyherbal formulation, Amrit oil is tested on an experimental model of the excisional wound in Wistar rats and evaluated for its potent activity in wound repair. Materials and Methods: Wistar rats were randomly arranged into 4 groups with 6 in each. Excision wound was created on the dorsal side of the rats. Group-I was kept as a negative control, Group-II as reference control, treated with Betadine, Group-III received a tropical application of Amrit oil once in a day and Group- IV were subjected to tropical application of amrit oil twice or Bis in a day. The wound healing was assessed by percentage wound contraction, a period of epithelisation, histopathological analysis and expression of MMP- 2, MMP-8, KGF and HSP-90. Results: The effect produced by the ayurvedic polyherbal formulation (Amrit oil), in terms of percentage wound contraction, a period of epithelisation, biochemical gene expression (MMP2, MMP8, HSP90 and KGF) and histopathological analysis were comparable to that of standard drug betadine. This study reveals the healing potential of Amrit oil as compared to the positive control, betadine possibly due to the presence of the phytochemicals in a synergistic manner. Conclusion: This study revealed a tremendous healing potential of Amrit oil as compared to the positive control, betadine in a holistic manner, confirming the rationality of the traditional knowledge.      

scholarly journals Expression of fibroblast cells after extraction of wistar rat teeth after topical application of okra fruit (Abelmoschus esculentus) gel

2020 ◽  
Author(s):  
Muhammad Luthfi ◽  
Wisnu Setyari Juliastuti ◽  
Yuniar Aliyah Risky ◽  
Elvina Hasna Wijayanti ◽  
Aisyah Ekasari Rachmawati ◽  
...  
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Wistar Rats ◽  
Tooth Extraction ◽  
Healing Process ◽  
Wistar Rat ◽  
Control Group ◽  
Wound Healing Process ◽  
Fibroblast Cells ◽  
Fruit Extract

Background: Tooth extraction is a dental procedure for removing a teeth from the alveolar bone socket. The tooth extraction process causes damage to hard tissue and soft tissue, so the body will respond physiologically to heal the wound. The wound healing process is divided into several phases, one of which is the proliferation phase of fibroblasts, which is one of the most important phases in the process of wound healing. Okra fruit contains saponins, tannins, flavonoids and alkaloids that have anti-inflammatory, antibacterial, antioxidant effects, and can stimulate angiogenesis so to accelerate the process of wound healing. Objective: to prove that the administration of okra fruit extract can accelerate the process of wound healing after extraction in the teeth of Wistar rats through increased expression of fibroblast cells. Methods: 18 Wistar rats were divided into 2 groups; control group and treatment group. The treatment group received a 30% okra fruit extract. The number of fibroblasts was calculated statistically using One Way ANOVA and Tukey HSD. Results: The results showed that the expression of control group fibroblast cells on day 3 (19.00 ± 2.0), day 5 (21.67 ± 2.08), day 7 (24.00 ± 2.00), whereas in the treatment group on day 3 (24.00 ± 1.00), day 5 (29.00± 2.00), day 7 (30.00 ± 1.53). Anova test between groups showed a significant difference with P-value 0.006. Conclusion: 30% okra fruit extract can increase fibroblast expression in wound healing process after extraction of Wistar rat teeth.

scholarly journals Ankaferd blood stopper accelerates deep second degree burn wound healing in rats

2018 ◽  
Vol 87 (3) ◽  
pp. 261-267 ◽  
Author(s):  
Ayse Topal ◽  
Nihal Y. Gul Satar ◽  
I. Taci Cangul ◽  
M. Ayberk Oktay ◽  
Kivanc Inan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Histopathological Examination ◽  
Wound Contraction ◽  
Control Group ◽  
Sprague Dawley ◽  
Burn Wound ◽  
Burn Wound Healing ◽  
Degree Burn ◽  
Ankaferd Blood Stopper ◽  
The Mean

In this study, the effects of Ankaferd Blood Stopper (ABS) and silver sulphadiazine (SSD) cream on burn wound healing were investigated in rats. A total of 24 outbred, male, Sprague-Dawley rats were randomly allocated to (1) ABS, (2) SSD, and (3) control groups. Bilateral burn wounds were created near the caudal border of the scapula. Wounds in each group were treated daily with sponges soaked in ABS solution, 1% SSD cream, or saline, respectively. On days 0, 7, 14, 21, and 28, unhealed wound area was measured and biopsy samples were taken for histopathological examination (except day 0). At the end of day 28, all rats in the ABS and SSD groups had complete coverage of the wounds with granulation tissue and epithelialization, whereas wounds in the control group were not completely epithelialized. On day 7, the mean unhealed wound areas and the mean percentages of wound contraction were not significantly different among the groups. However, the mean percentage of wound contraction in the ABS and SSD groups was significantly higher than in the control group on days 14, 21, and 28. Histopathologically, wound healing was characterized by a decrease in neutrophil counts and an increase in vessel counts. Our results suggest that ABS can be successfully used for burn wound healing besides SSD.

scholarly journals A topical ointment formulation containing leaves' powder of Lawsonia inermis accelerate excision wound healing in Wistar rats

2020 ◽  
Vol 13 (7) ◽  
pp. 1280-1287
Author(s):  
Kalbaza Ahmed Yassine ◽  
Hemida Houari ◽  
Benchohra Mokhtar ◽  
Amara Karim ◽  
Salem Hadjer ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Histological Study ◽  
Test Group ◽  
Wound Contraction ◽  
Control Group ◽  
Petroleum Jelly ◽  
Lawsonia Inermis ◽  
Wound Healing Activity ◽  
Daily Application

Aim: Lawsonia inermis (LI), a naturally grown or cultivated shrub in Northeast of Africa and India, has been traditionally used as a strong remedy for several injuries. However, few studies have reported its use as a cicatrizing agent. The aim of this study was to evaluate the effect of daily application of an ointment prepared with LI leaves' powder on wound healing in Wistar rats. Materials and Methods: Twenty female Wistar rats were used in this study. Excisional wound model was realized by removing skin from the dorsal part of the neck of each animal. Wounds have been then treated by a daily application of LI ointment prepared by mixing leaves' powder to petroleum jelly in test group and by simple application of petroleum jelly in control group. Evaluation of wound healing activity was then based on calculating the percentage of wound contraction, period of epithelialization, and wound index every 3 days for a period of 24 days, then, a histological study of the healed excised wound was performed. Results: Treatment with LI has shown excellent wound healing activity, since it has increased percent of wound contraction, and reduced period of epithelialization and wound index as compared to control (p<0.05). These results have been supported by the histological findings that revealed better epithelialization, dermal differentiation, collagen fiber orientation, and angiogenesis in LI treated rats compared to control (p<0.05). Conclusion: We can conclude that LI leaves' can be used as a potential wound healing agent.

Sign in / Sign up

Export Citation Format

Share Document