scholarly journals Assessment of the Antibacterial Property of Mineral Trioxide Aggregate and Glass Ionomer Cement Mixture

Author(s):  
Ankita Komal Labh ◽  
Anjaneyulu K. ◽  
Muralidharan N. P.

Mineral trioxide aggregate (MTA) is an experimental material used for the capping of pulps as well as a root end filling material. Glass ionomer cement (GIC) is a widely used dental restorative due to its high mechanical strength and antibacterial function. The aim of this study is to assess the antibacterial property of MTA when mixed with GIC. The study comprised four groups with 5 samples each: Group A (MTA), group B (GIC), groups C (MTA+GIC+MTA Liquid) and group D (MTA+GIC+GIC Liquid). All samples were tested against Enterococcus faecalis using Agar well diffusion method in Tryptone soya agar. The One-way ANOVA test was employed using the Statistical Package for Social Sciences (SPSS) for MacOS (Version 19, 2010) with the level of significance set at P<0.05. The average zone of inhibition of group A was 18.6 ± 1.714 mm, group B was 0.4 ± 0.548 mm, group C was 18.6 ± 2.608 mm and group D was 12.8 ± 2.168 mm. Data was statistically significant, F(3,16)=11.631, P<0.001. The antibacterial activity of MTA and MTA when mixed with GIC and MTA liquid is similar but better than other study groups.

2013 ◽  
Vol 56 (3) ◽  
pp. 97-103 ◽  
Author(s):  
Prabath Singh ◽  
Joseph Paul ◽  
Abdul Aziz Al-Khuraif ◽  
Sajith Vellappally ◽  
Hassan Suliman Halawany ◽  
...  

Objectives: The purpose of this study was to evaluate the in vitro sealing ability of three repair materials. Mineral trioxide aggregate (MTA; Group A), calcium phosphate cement (CPC; Group B), and light cured glass ionomer cement (GIC; Group C) when used to repair the perforation created in the pulpal floor of fifty extracted human permanent molars. Materials and methods: Preparation of access openings and furcation perforations were done, and the teeth divided into five experimental groups (A, B, C) including two controls (D, E) with ten samples in each group randomly. Following the repair procedure, the pulp chambers and access openings were filled with composite resin and immersed in 2% methylene blue solution for 48 hours. The teeth were sectioned longitudinally and the linear dye penetration measured under a stereo­microscope. Results: The comparison of the linear length of micro-leakage (mm) among the experimental groups revealed no significant difference (p = 0.332). On calculating the percentage of depth of leakage to the total length of the perforation, it was observed that the mean leakage was 35.5% in Group A, 53.6% in Group B and the highest, 87.5% in Group C. The mean of leakage percentage was statistically significant by Kruskal-Wallis test (p = 0.003). The results indicated that the dye penetration used as furcation perforation repair material was least with mineral trioxide aggregate. Comparing the depth of penetration of dye, 50% of the Group A samples showed less than 25% of depth penetration. While 40% of Group B cases had more than 50% dye penetration. In our study, all Group C teeth had ≥ 50% dye penetration. Conclusions: The present study indicated that GIC had the greatest dye penetration followed by CPC and MTA. Mineral trioxide aggregate and calcium phosphate cement had comparatively better sealing ability than glass ionomer cement.


Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 528
Author(s):  
Amal Adnan Ashour ◽  
Sakeenabi Basha ◽  
Nayef H. Felemban ◽  
Enas T. Enan ◽  
Amal Ahmed Alyamani ◽  
...  

In the present study, Zingiber officinale is used for the synthesis of Zingiber officinale capped silver nanoparticles (ZOE-AgNPs) and compares the antimicrobial efficacy and compressive strength of conventional glass ionomer cement (GIC) combined with ZOE-AgNPs, lyophilized miswak, and chlorhexidine diacetate (CHX) against oral microbes. Five groups of the disc-shaped GIC specimens were prepared. Group A: lyophilized miswak and GIC combination, Group B: ZOE-AgNPs and GIC combinations, Group C: CHX and GIC combination, Group D: ZOE-AgNPs + CHX + GIC; Group E: Conventional GIC. Results confirmed the successful formation of ZOE-AgNPs that was monitored by UV-Vis sharp absorption spectra at 415 nm. The X-ray diffractometer (XRD) and transmission electron microscope (TEM) results revealed the formation of ZOE-AgNPs with a mean size 10.5–14.12 nm. The peaks of the Fourier transform infrared spectroscopy (FTIR) were appearing the involvement of ZOE components onto the surface of ZOE-AgNPs which played as bioreducing, and stabilizing agents. At a 24-h, one-week and three-week intervals, Group D showed the significantly highest mean inhibitory zones compared to Group A, Group B, and Group C. At microbe-level comparison, Streptococcus mutans and Staphylococcus aureus were inhibited significantly by all the specimens tested except group E when compared to Candida albicans. Group D specimens showed slightly higher (45.8 ± 5.4) mean compressive strength in comparison with other groups. The combination of GIC with ZOE-AgNPs and chlorhexidine together enhanced its antimicrobial efficacy and compressive strength compared to GIC with ZOE-AgNPs or lyophilized miswak or chlorhexidine combination alone. The present study revealed that The combination of GIC with active components of ZOE-AgNPs and chlorhexidine paves the way to lead its effective nano-dental materials applications.


Materials ◽  
2021 ◽  
Vol 14 (18) ◽  
pp. 5242
Author(s):  
Gabriela Ciavoi ◽  
Ruxandra Mărgărit ◽  
Liana Todor ◽  
Dana Bodnar ◽  
Magdalena Natalia Dina ◽  
...  

The aim of this study was to compare fracture resistance of teeth presenting medium-sized mesial-occlusal-distal (MOD) cavities using different base materials. Thirty-six extracted molars were immersed for 48 h in saline solution (0.1% thymol at 4 °C) and divided into six groups. In group A, the molars were untouched, and in group B, cavities were prepared, but not filled. In group C, we used zinc polycarboxylate cement, in group D—conventional glass ionomer cement, in group E—resin modified glass ionomer cement, and in group F—flow composite. Fracture resistance was tested using a universal loading machine (Lloyd Instruments) with a maximum force of 5 kN and a crosshead speed of 1.0 mm/min; we used NEXYGEN Data Analysis Software and ANOVA Method (p < 0.05). The smallest load that determined the sample failure was 2780 N for Group A, 865 N for Group B, 1210 N for Group C, 1340 N for Group D, 1630 N for Group E and 1742 N for Group F. The highest loads were 3050 N (A), 1040 N (B), 1430 N (C), 1500 N (D), 1790 N (E), and 3320 N (F), the mean values being 2902 ± 114 N (A), 972 ± 65 N (B), 1339 ± 84 N (C), 1415 ± 67 N (D), 1712 ± 62 N (E), and 2334 ± 662 N (F). A p = 0.000195 shows a statistically significant difference between groups C, D, E and F. For medium sized mesial-occlusal-distal (MOD) cavities, the best base material regarding fracture resistance was flow composite, followed by glass ionomer modified with resin, conventional glass ionomer cement and zinc polycarboxylate cement. It can be concluded that light-cured base materials are a better option for the analyzed use case, one of the possible reasons being their compatibility with the final restoration material, also light-cured.


2018 ◽  
Vol 6 (3) ◽  
pp. 40
Author(s):  
Gehan Allam ◽  
Ola Abd El-Geleel

The aim of this study was to test the effect of adding chicken eggshell powder (CESP) to conventional glass-ionomer cement (GIC) on its mechanical properties, and fluoride and calcium release. CESP was added with proportions of 3% and 5% by weight to the powder component of conventional glass-ionomer cement. The specimens were categorized into group A: GIC without CESP; group B: GIC with 3% wt. CESP; and group C: GIC with 5% wt. CESP; there were 12 specimens in each group. Groups B and C showed higher compressive strength values compared to group A. However, microhardness scores were higher in group C compared to groups A and B. As for ion-release results, group B displayed the highest values of fluoride release followed by group C at both 7 and 30 days. Group C showed the highest amount of calcium release followed by both groups B and C at 7 days, while at 30 days, groups A and B showed higher calcium release compared to group C. The mechanical properties of conventional glass-ionomer restorative material were enhanced by the addition of CESP. Moreover, fluoride and calcium release were not compromised by adding CESP.


2019 ◽  
Vol 7 (1) ◽  
pp. 2 ◽  
Author(s):  
Marc Krikor Kaloustian ◽  
Walid Nehme ◽  
Claire El Hachem ◽  
Carla Zogheib ◽  
Nabil Ghosn ◽  
...  

We assessed the efficiency of two shaping file systems and two passive ultrasonic irrigation (PUI) devices for removing filling material during retreatment. The mesial canals from 44 extracted mandibular molars were prepared and obturated. The teeth were randomly divided into two groups, and then one group was retreated with Reciproc R25 (VDW, Munich, Germany) (n = 44) and the other group was retreated with 2Shape (TS, Micro Mega, Besançon, France) (n = 44). A micro-computed tomography (CT) scan was taken before and after the retreatment to assess the volume of the filling material remnants. The teeth were then randomly divided into four groups to test two different PUI devices: Irrisafe (Satelec Acteon Group, Merignac, France) and Endo Ultra (Vista Dental Products, Racine, WI, USA). The teeth in Group A were retreated with 2Shape to test the Endo Ultra (n = 22) device, the teeth in Group B were retreated with 2Shape in order to test the Irrisafe (n = 22) device, the teeth in Group C were retreated with Reciproc to test the Endo Ultra (n = 22) device, and Group D was retreated with Reciproc to test the Irrisafe (n = 22) device. A third micro-CT scan was taken after the retreatment to test the PUIs. The percentage of Gutta-Percha (GP) and sealer removed was 94.75% for TS2 (p < 0.001) and 89.3% for R25 (p < 0.001). The PUI significantly enhanced the removal of the filling material by 0.76% for Group A (p < 0.001), 1.47% for Group B (p < 0.001), 2.61% for Group C (p < 0.001), and by 1.66% for Group D (p < 0.001). 2Shape was more effective at removing the GP and sealer during retreatment (p = 0.018). The supplementary approach with PUI significantly improved filling material removal, with no statistical difference between the four groups (p = 0.106).


2015 ◽  
Vol 62 (1) ◽  
pp. 7-13 ◽  
Author(s):  
Sameer Makkar ◽  
Ruchi Vashisht ◽  
Anita Kalsi ◽  
Pranav Gupta

Summary Introduction Throughout the history of dentistry, a wide variety of materials such as gold-foil, silver posts, amalgam, zinc oxide eugenol, glass ionomer cements, mineral trioxide aggregate have been used as retrograde fillings. Altered pH in periapical lesions can affect push-out bond strength of these materials. The aim of this study was to evaluate the effect of altered pH on push-out bond strength of Biodentin, Glass ionomer cement (GIC), Mineral trioxide aggregate (MTA) and Theracal. Material and Methods Forty-eight dentin slices of extracted single-rooted human teeth were sectioned and their canal portion instrumented to achieve a diameter of 1.4 mm. The specimens were then assigned into the four groups (one group for each material) with 12 samples in each group. All groups were further divided into 3 subgroups (with 4 specimens in each subgroup): acidic (butyric acid buffered at pH 6.4), neutral (phosphate buffer saline solution at pH 7.4) and alkaline (buffered potassium hydroxide at pH 8.4). Samples were incubated for 4 days at 37°C in acidic, neutral or alkaline medium. Push-out bond strength was measured using a Universal Testing Machine. The slices were examined under a stereomicroscope to determine the nature of bond failure. Results GIC showed the highest bond strength (33.33MPa) in neutral and acidic medium (26.75MPa) compared to other materials. Biodentin showed the best result in alkaline medium. Conclusion Altered pH level affected push-out bond strength of root end materials. GIC demonstrated good push-out bond strength that increased with decrease of pH whereas newer materials Biodentin and Theracal showed satisfying results in altered pH.


2009 ◽  
Vol 34 (2) ◽  
pp. 157-160 ◽  
Author(s):  
Mesut Enes Odabas ◽  
Ozlem Tulunoglu ◽  
Serife Ozdemir Ozalp ◽  
Haluk Bodur

Objective: The aim of this study was to compare the sealing properties of IRM, Coltosol, Cavit G, Adhesor and Clip, which used as temporary filling material in coronal access openings in extracted human primary teeth. Study Design: Standardized access cavities of 2×2 mm were prepared in the eighty-four, caries-free human primary anterior teeth. The teeth were divided randomly into five groups of 16 teeth each. Temporary restorative materials Group A: IRM (Dentsply), Group B: Coltosol (Coltone), Group C: Cavit G (3M),Group D: Adhesor (Spofa Dental) and Group E: Clip (Voco) were applied according to the manufacturer's directions. The specimens were immersed silver nitrate and placed in film developer under fluorescent for 24 hours. The sectioned specimens were evaluated under a digital microscope at x 20 magnifications and blindly scored for microleakage. Results: Clip presented the least microleakage value whereas; Adhesor and IRM presented the higher microleakage values. There were statistically significant differences between Clip and the others groups, while there were no statistically significant differences in microleakage between IRM,Adhesor, Coltosol and Cavit G. However, the leakage scores of Clip and Cavit G were congruent (p= 0.454).Conclusion: Amongst the five materials, Clip exhibited a better sealing ability.


2021 ◽  
Vol 20 ◽  
pp. e213981
Author(s):  
Fariba Motevasselian ◽  
Hamid Kermanshah ◽  
Ebrahim Rasoulkhani ◽  
Mutlu Özcan

Aim: To compare the microleakage of Cention N, a subgroup of composite resins with a resin-modified glass ionomer (RMGI) and a composite resin. Methods: Class V cavities were prepared on the buccal and lingual surfaces of 46 extracted human molars. The teeth were randomly assigned to four groups. Group A: Tetric N-Bond etch-and-rinse adhesive and Tetric N-Ceram nanohybrid composite resin, group B: Cention N without adhesive, group C: Cention N with adhesive, and group D: Fuji II LC RMGI. The teeth were thermocycled between 5°-55°C (×10,000). The teeth were coated with two layers of nail vanish except for 1 mm around the restoration margins, and immersed in 2% methylene blue (37°C, 24 h) before buccolingual sectioning to evaluate dye penetration under a stereomicroscope (×20). The data were analyzed by the Kruskal-Wallis and Wilcoxon tests (α=0.05). Results: Type of material and restoration margin had significant effects on the microleakage (p<0.05). Dentin margins showed a higher leakage score in all groups. Cention N and RMGI groups showed significant differences at the enamel margin (p=0.025, p=0.011), and for the latter group the scores were higher. No significant difference was found at the dentin margins between the materials except between Cention N with adhesive and RMGI (p=0.031). Conclusion: Microleakage was evident in all three restorative materials. Cention N groups showed similar microleakage scores to the composite resin and displayed lower microleakage scores compared with RMGI.


2020 ◽  
Author(s):  
Enas Tawfik Enan ◽  
Amal A. Ashour ◽  
Sakeenabi Basha ◽  
Nayef H. Felemban ◽  
sanaa M. F. gad El-Rab

Abstract Background. The development of dental caries is associated with various microorganisms and secondary caries formation is the main cause of restorations failure. The advise for restorative dental materials that have antimicrobial properties has stimulated the introduction of materials containing different antibacterial agents.Objectives: Present study has been designed to synthesize silver nanoparticles (AgNPs) and incorporate AgNPs and amoxicillin into glass ionomer cement (GIC) to synergize its effect on oral microbes. The effect of the added antimicrobial agents on compressive strength (CS) of GIC was also evaluated.Material and methods: Biosynthesis of AgNPs were done using Cupressus macrocarpa extract and AgNPs were charectatrized. A total of 120 disc-shaped specimens were prepared and classified into 4 main groups where Group A include conventional GIC, Groups B and C include GIC with AgNPs or Amoxicillin, respectively, while Group D included GIC with both AgNPs and Amoxicillin. Each group was tested for the antimicrobial activity against both Streptococcus mutans (S. mutans) and Staphylococcus aureus (S. aureus). The distribution of biofilm was examined via scanning electron microscope. The CS of the tested material was measured using a Material Test System(MTS).Results: UV-Visible spectrum showed a peak of 429 nm. TEM, XRD pattern and FTIR analysis confirmed the formation of AgNPs with spherical to oblong polydispersed particles of diameter in the range of 13.5-25.8 nm. The maximum inhibitory zone was recorded for group D against both tested bacteria with a mean of 29 mm at first 24-hour period to 15 mm at three weeks and showed antimicrobial rate 92.2% and 92.56%, against both strains, respectively. Additionally, group D disintegrated the structure of S. aureus biofilm and even kill bacteria in the biofilms. Addition of AgNPs and Amoxicillin caused an insignificant effect on CS of GIC.Conclusion: TheAgNPs showed a synergistic effect in combination with amoxicillin and GIC dental restorative material against studied microorganisms. The agents can be safely added with minimal effect on mechanical properties of the original cement.


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