scholarly journals Use of Origanum vulgare Essential Oil as an Antibacterial Additive in the Preservation of Minced Meat

2020 ◽  
pp. 1-9
Author(s):  
Sonia Heni ◽  
Hicham Boughendjioua ◽  
Meliani Saida ◽  
Salima Bennadja ◽  
Abdelghani Djahoudi
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Nutritional Value ◽  
Reduction Rate ◽  
Microbiological Quality ◽  
Gas Chromatography Mass Spectrometry ◽  
Origanum Vulgare ◽  
North East ◽  
Aerial Parts ◽  
Minced Meat

The essential oil extracted by hydrodistillation from the aerial parts of Origanum vulgare L. harvested in the region of Skikda (North-East-Algeria) gave an excellent oil yield (2.8%). Its analysis by Gas Chromatography–Mass Spectrometry (GC/MS) identified 98.10% of its constituents. The major components are: carvacrol (47.6%), thymol (16.6%), p-cymene (13.5%) and γ-terpinene (11.2%). The aim of this study was to the preservative effect of Origanum vulgare essential oil applied to a very low concentration of 0.025% (minimum inhibitory concentration (MIC)), of a sensitive food of essential nutritional value, of great consumption and easily perishable “minced meat”, stored at different times, namely: T0 = 0 min, T1 = 40 min, T2 = 24 h, and T3 = 48 h. By studying its microbiological quality by determining the rate of reduction of the total aerobic mesophilic microflora and of Staphylococcus aureus. The addition of Origanum vulgare essential oil to minced meat allowed a highly significant reduction of 0.01<p<0.1 in total aerobic mesophilic microflora reduction rate and very highly significant (p≤0.01) for Staphylococcus aureus, and markedly increase the shelf life. This allows us to propose the use of this essential oil as a source of natural preservative substances.

scholarly journals A Carvacrol-Rich Essential Oil Extracted From Oregano (Origanum vulgare “Hot & Spicy”) Exerts Potent Antibacterial Effects Against Staphylococcus aureus

2021 ◽  
Vol 12 ◽  
Author(s):  
Yuanpeng Hao ◽  
Jingyi Li ◽  
Lei Shi
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Cell Damage ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Major Constituent ◽  
Lag Phase ◽  
Pathway Enrichment Analysis ◽  
Antibacterial Effects ◽  
Origanum Vulgare

Oregano essential oil (OEO), as a natural antimicrobial, has gained increased interest from food researchers and manufacturers. However, a few studies have investigated its possible antibacterial effects against Staphylococcus aureus using the proteomic tool. The present study aimed to explore the antibacterial effect and mechanism of a carvacrol-rich OEO extracted from Origanum vulgare “Hot &amp; Spicy” on the inactivation of S. aureus. The gas chromatography–mass spectrometry analysis of the OEO allowed the detection of 27 compounds; the major constituent was carvacrol (84.38% of total compounds). The average diameter of the inhibitory zone (DIZ) value was 29.10 mm, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of OEO against S. aureus were 0.125 and 0.25 mg/mL, respectively. The growth curve assay indicated that the OEO prolonged the lag phase of S. aureus. The decrease in cell viability, changes in the integrity of cell membrane, and abnormal cell morphology further reflected the cell damage of S. aureus caused by the OEO. In addition, a label-free proteomic analysis was applied to analyze the regulatory networks of S. aureus in response to 1/2 MIC OEO-treatment stress. Of the 56 differentially expressed proteins (DEPs) identified, 26 were significantly upregulated and 30 downregulated. The Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that the DEPs were mainly involved in pathways of ribosomes; valine, leucine, and isoleucine biosynthesis; and phenylalanine, tyrosine, and tryptophan biosynthesis, which suggested that the growth inhibition of S. aureus might be due to the disordered effect of the OEO on protein synthesis and amino acid metabolism. These findings deepened our understanding regarding S. aureus survival and metabolism responses to the OEO treatment and suggested that the carvacrol-rich OEO could be used in food production environments to effectively control S. aureus.

scholarly journals Combination of Origanum vulgare L. essential oil and lactic acid to inhibit Staphylococcus aureus in meat broth and meat model

2012 ◽  
Vol 43 (3) ◽  
pp. 1120-1127 ◽  
Author(s):  
Jefferson C. de Barros ◽  
Maria Lúcia da Conceição ◽  
Nelson Justino Gomes Neto ◽  
Ana Caroliny Vieira da Costa ◽  
Evandro Leite de Souza
Keyword(s):  
Staphylococcus Aureus ◽  
Lactic Acid ◽  
Essential Oil ◽  
Origanum Vulgare

scholarly journals Chemical composition and antibacterial activities of essential oils from fruits of Melicope pteleifolia (Champ. Ex Benth.) T.G. Hartley grown in Lam Dong Province, Vietnam

2020 ◽  
Vol 42 (3) ◽  
Author(s):  
Hoang Thi Binh ◽  
Tran Thi Bao Tram ◽  
Do Ngoc Dai ◽  
Vuong Thuy Tien ◽  
Le Minh Tam ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Chemical Composition ◽  
Essential Oil ◽  
Streptococcus Pyogenes ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Aerial Parts ◽  
Agar Well Diffusion Method

In the present study, chemical composition and antibacterial properties of essential oil obtained from the aerial parts of the Melicope pteleifolia (Champ. ex Benth.) T.G Hartley in Dalat were evaluated. Essential oil was isolated through hydro-distillation. Twenty-nine constituents comprising 100% of the essential oil were characterized by gas chromatography/mass spectrometry (GC-MS) techniques. The major compounds in the essential oil were (+)-Sabinene (34.73%), Cis-α-bergamotene (13.15%), Z-α-trans-bergamotol (5.28%), β-mycrene (4.98%), and 1,3,6-octatriene, 3,7-dimethyl-(4.71%). Antibacterial activities of Melicope pteleifolia essential oil were investigated against Gram-positive and-negative bacteria. Results showed significant activities against Streptococcus pyogenes and Escherichia coli using an agar well diffusion method. The application of this essential oil in preventing and eliminating bacteria could be useful in fields as medicine and cosmetics. 

scholarly journals Antibacterial Activity of Endostemon tereticaulis (Poir.) M. Ashby Essential Oil and Ethanolic Extract Against Resistant Pathogenic Bacteria

2020 ◽  
Vol 15 (9) ◽  
pp. 1934578X2095325
Author(s):  
Bala Namata Abba ◽  
Abderrahmane Romane ◽  
Amadou Tidjani Ilagouma
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Traditional Medicine ◽  
Pathogenic Bacteria ◽  
Ethanolic Extract ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Minimal Bactericidal Concentration

Endostemon tereticaulis (poir.) M.Ashby is a species of the Lamiaceae family present in Niger. This plant is used in traditional medicine due to its various biological potentialities. The present study investigated the chemical composition of the essential oil and the antibacterial activity of the essential oil and ethanolic extract of Endostemon tereticaulis against resistant pathogenic bacteria. Gas chromatography-mass spectrometry analysis of the essential oil led to the identification of 43 compounds representing 99.55% of the total essential oil. The major components were caryophyllene oxide (15.17%) followed by α-humulene (13.96%), α-copaene (11.75%), ( E)-β-caryophyllene (8.44%), and δ-cadinene (6.78%). The antibacterial activity was tested against multiresistant Acinetobacter baumannii P1483, Salmonella spp. H1548, extended-spectrum β-lactamase- Escherichia coli Bu8566, Enterobacter cloacae Bu147, Proteus mirabilis Bu190 , Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 700603), Escherichia coli (ATCC 25922), Enterococcus faecium H3434, methicillin-resistant Staphylococcus aureus P1123, and Staphylococcus aureus (ATCC 25923). The antibacterial assays revealed that the essential oil was more active than the ethanolic extract against the studied bacteria with minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values ranging from 0.06 to 2 mg/mL. Also, the ethanolic extract was effective against the bacteria tested with MIC and MBC values ranging from 0.12 to 3 mg/mL. This study showed that Endostemon tereticaulis essential oil is rich in bioactive compounds. Ethanolic extract and essential oil exhibited potential antibacterial activity. These results provide a scientific basis for the use of this plant in traditional medicine. The current study described for the first time the antibacterial activity of Endostemon tereticaulis.

scholarly journals Chemical Composition of the Essential Oil of Croton bonplandianus from India

2014 ◽  
Vol 9 (2) ◽  
pp. 1934578X1400900
Author(s):  
Rajesh K. Joshi
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Gas Chromatography Mass Spectrometry ◽  
Caryophyllene Oxide ◽  
Aerial Parts ◽  
Sesquiterpene Hydrocarbons ◽  
Germacrene D ◽  
Total Oil

The essential oil obtained from the aerial parts of Croton bonplandianus Baill. was analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). A total of 37 compounds have been identified, representing 96.2% of the total oil. The main constituents were identified as β-caryophyllene (16.7%), germacrene D (14.7%), borneol (8.3%), Z-β-damascenone (6.(%), isobornyl acetate (6.2%), α-humulene (6.1%), germacrene A (5.2%) and caryophyllene oxide (4.5%). The oil was rich in sesquiterpene hydrocarbons (60.1%).

scholarly journals Chemical investigation of the essential oil of Laggera crispata (Vahl) Hepper & Wood from India

2011 ◽  
Vol 76 (4) ◽  
pp. 523-528 ◽  
Author(s):  
Ram Verma ◽  
Rajendra Padalia ◽  
Chandan Chanotiya ◽  
Amit Chauhan ◽  
Anju Yadav
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Essential Oil ◽  
Chemical Investigation ◽  
Gas Chromatography Mass Spectrometry ◽  
Western Himalayas ◽  
Caryophyllene Oxide ◽  
Oil Composition ◽  
Aerial Parts ◽  
Total Oil

Hydrodistilled essential oil of the aerial parts of Laggera crispata (Vahl) Hepper & Wood, collected from the Kumaon region of the western Himalayas was analysed by gas chromatography and gas chromatography-Mass Spectrometry. Eighty constituents, accounting for 83.9 % of the total oil composition, were identified. The oil was mainly dominated by sesquiterpenoids (45.3 %) and benzenoid compounds (33.9 %). Among them, 2,5-dimethoxy-p-cymene (32.2 %), 10-epi-?-eudesmol (14.7 %), ?-caryophyllene (6.9 %), and caryophyllene oxide (5.4 %) were major components of the oil.

Chemical Composition of the Essential Oil From Minthostachys acris Schmidt-Leb. Grown in the Peruvian Andes

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1985749
Author(s):  
Jorge A. Pino ◽  
María Milagros Dueñas-Mendoza ◽  
Leoncio Solís-Quispe
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Steam Distillation ◽  
Gas Chromatography Mass Spectrometry ◽  
Ionization Detector ◽  
Peruvian Andes ◽  
Flame Ionization ◽  
Aerial Parts

The chemical composition of the essential oil from aerial parts of Minthostachys acris Schmidt-Leb. grown in Cuzco was studied. A total of 59 volatile compounds were identified by gas-chromatography-flame ionization detector and gas chromatography-mass spectrometry in the essential oil obtained by steam distillation, of which the most prominent were pulegone (54.4%), cis-menthone (11.0%), and thymol (6.3%).

scholarly journals Chemical Composition of the Essential Oil of Baccharoides lilacina from India

2013 ◽  
Vol 8 (3) ◽  
pp. 1934578X1300800
Author(s):  
Rajesh K. Joshi
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Essential Oil ◽  
Essential Oil Composition ◽  
Gas Chromatography Mass Spectrometry ◽  
Caryophyllene Oxide ◽  
Oil Composition ◽  
Aerial Parts ◽  
Sesquiterpene Hydrocarbons ◽  
Total Oil

The essential oil composition from the aerial parts of Baccharoides lilacina (Dalzell & A. Gibson) M. R. Almeida was analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). A total of 41 compounds have been identified, representing 97.4% of the total oil. The main constituents were identified as β-caryophyllene (27.7%), epi-α-cadinol (25.1%), caryophyllene oxide (9.9%), α-muurolol (7.6%), α-cadinene (6.1%) and α-cadinol 4.5%). The oil was found to be rich in oxygenated sesquiterpenes (47.1%) and sesquiterpene hydrocarbons (46.2%).

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