scholarly journals Antioxidant and Xanthine Oxidase Inhibitory Potential of Aqueous Extract of Ferula Asafoetida

2021 ◽  
pp. 166-173
Author(s):  
G. G. Gopika ◽  
R. Gayathri ◽  
V. Vishnu Priya ◽  
J. Selvaraj ◽  
S. Kavitha
Keyword(s):  
Antioxidant Activity ◽  
Xanthine Oxidase ◽  
Aqueous Extract ◽  
Dependent Manner ◽  
Phytochemical Screening ◽  
Standard Drug ◽  
Inhibitory Potential ◽  
Ferula Asafoetida ◽  
In Vitro Antioxidant Activity

Background: Ferula asafoetida is a dried latex that is exuded from rhizome or taproot species. Organosulfides are primarily responsible for flavour and odour of asafoetida.Ferula asafoetida is a natural medicine good for asthma and bronchitis. is also used to relieve stomach gas, digestive issues. It is usually added while cooking to  hormonise the sweet, sour, salty, spicy taste of the food. Increased activity of xanthine oxidase is involved in the medical condition known as gout, which is characterized by hyperuricemia that leads to deposition of uric acid in the joints resulting in painful inflammation. Aim: To analyse the anti-oxidant and xanthine oxidase inhibitory potential of aqueous extract of Ferula asafoetida. Materials and Methods: Preparation of the aqueous seed extract of Ferula asafoetida  done by hot percolation method. Phytochemical screening, in vitro antioxidant activity and xanthine oxidase inhibitory potential was done by standard procedures. The data were analyzed statistically by a one - way analysis of variance (ANOVA) followed by Duncan’s multiple range test was used to see the statistical significance among the groups. The results with the p<0.05 level were considered to be statistically significant. Results: The phytochemical screening revealed that the extract is rich in phytoconstituents. DPPH radical scavenging activity established the potent in vitro antioxidant activity (p<0.05) of Ferula asafoetida extract. The extract was also efficient in inhibiting the activity of xanthine oxidase enzyme (p<0.05) in a concentration dependent manner. Discussion: The extract has potent antioxidant and xanthine oxidase inhibitory potential, although the activities are less compared to the standard drug. Conclusion: The Ferula asafoetida extract can be used to treat gout and to combat various other disorders associated with xanthine oxidase activity.

scholarly journals Antioxidant and antiproliferative properties of Moringa oleifera Lam. leaf aqueous extract

2020 ◽  
Vol 7 (4) ◽  
Author(s):  
D Athira Nair ◽  
T J James ◽  
S L Sreelatha ◽  
Bibu John Kariyil
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Moringa Oleifera ◽  
Dependent Manner ◽  
Standard Drug ◽  
In Vivo Analysis ◽  
Moringa Oleifera Lam

Moringa oleifera Lam. is a highly valued medicinal plant in India, especially Kerala. In the present study, antioxidant activity of aqueous extract of leaves of M. oleifera was determined both in-vitro and in-vivo. Male Wistar rats of 3 age groups- 6, 12, and 18 months old were used for in-vivo analysis. In vitro anti-proliferative effect of the extract was carried out in Dalton’s Lymphoma Ascites (DLA) Cells. LCMS-QTOF analysis of the extract was also done to determine the bioactive components present in the extract. Antioxidant activity of M. oleifera leaf showed an IC 50 value of 10.47 ?g/ml and whereas for standard drug, ascorbic acid, it was 19.52 ?g/ml. In-vivo analysis of lipid peroxidation showed a significant reduction of lipid peroxidation in the brains of 12 and 18-months old treated groups. Up to 75% mortality of DLA cancerous cells was observed in-vitro in different concentrations of M. oleifera leaf water extract in a dose-dependent manner, demonstrating its anti-proliferative property. LCMS-QTOF analysis revealed the presence of emodin-8-glucoside in the extract. Molecular docking analysis (Auto Dock Vina) of emodin-8-glucoside with six cancer related proteins showed highest binding affinity with AKT-1 with a binding score of -10.4 kcal/mol, also showed good affinity with NF-kB (p65), Stat-3, Bcl-2, Bcl-xl and c-FLIP. This study helps to choose healthy diet practices to overcome free radical onslaught and cancerous cell proliferation especially in the later stages of life. This can also pave way for the emergence of diet based therapeutic cure for cancer.

scholarly journals Phytochemical Screening and In-Vitro Antioxidant Activity of Peristrophe paniculata

2018 ◽  
Vol 04 (01) ◽  
Author(s):  
Srikanth M ◽  
Devi B ◽  
Kotirataiah K ◽  
Ramanjaneyulu M ◽  
Sulthana PN ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phytochemical Screening ◽  
In Vitro Antioxidant Activity

scholarly journals Phytochemical, physicochemical, TLC, minerals analysis and in-vitro antioxidant activity of ethanolic extract of leaves of Heldigardia populifolia

2018 ◽  
Vol 1 (1) ◽  
pp. 22-26
Author(s):  
T Bhanumathi ◽  
P Keerthana ◽  
A Cheenakesavulu ◽  
M Neeharika ◽  
E Sandhya ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Permissible Limits ◽  
Leaf Powder ◽  
Composition Of Minerals ◽  
Tlc Analysis ◽  
In Vitro Antioxidant Activity

The aim of present study was to investigate the preliminary phytochemical, physicochemical, TLC, minerals analysis and In-vitro antioxidant activity of leaves of ethanolic extract of Heldigardia populifolia. The preliminary phytochemical screening of ethanolic extract showed the presence of triterpenoids, flavonoids, glycosides, sterols, steroids, phenols, carbohydrates and saponins. The composition of minerals found in the leaf powder was within the permissible limits. TLC analysis of ethanol extract showed the five spots which indicate the presence of five phytoconstituents. The extractive value of ethanol was high than acetone. Ash values were within the limits. The in-vitro antioxidant activity of ethanolic extract increased with increasing the concentration. The ethanolic extract in all the concentration showed the significant antioxidant activity.

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