scholarly journals Advanced Identification and Characterization of Listeria Species in Egyptian Local Soft Cheese

2020 ◽  
pp. 11-18
Author(s):  
Amany N. Dapgh ◽  
A. A. ELGedawy ◽  
Hussien A. Abouelhag ◽  
Asmaa S. Mansour ◽  
E. S. Gaber ◽  
...  
Keyword(s):  
Public Health ◽  
Antimicrobial Susceptibility ◽  
Raw Milk ◽  
Molecular Techniques ◽  
Health Concern ◽  
Public Health Concern ◽  
Advanced Technique ◽  
Soft Cheese ◽  
Vitek 2 ◽  
Species Specific

Aims: one of the most important foodborne microorganisms is the Gram’s positive environmental wide spread Listeria spp. As the Listeria may be considered a public health concern so there is in needing to rapid, precise and reliable diagnosis of the organism in consumed food. The present study aimed to survey the presence of Listeria spp. among two popular consuming Egyptian white soft cheese using advanced biochemical, antibiotic susceptibility and molecular techniques. Methodology: Listeria spp. was investigated in 155 samples of two white soft cheeses (70 kareish cheese and 85 Damietta cheese) collected from street vendors and retail markets in Giza. The existence of Listeria spp. was tested through cultural and the identification was confirmed biochemically by Vitek2 compact system as well as molecular identification via diplex real time PCR using species specific primers. Results: The results of the study revealed the isolation of two Listeria spp. in a total number of 22 from155 samples (14.19%); 14 isolate out of 70 (20%) Kareish cheese while 8 isolates out of 85 (9.4%) Damietta cheese's samples. The 22 Listeria spp. isolates were differentiated into L. innocua 15 (68.18%), and L. monocytogenes 7 (31.81%) also their antimicrobial susceptibility was declared using advanced Vitek-2 compact system. The two Listeria spp. isolates were definitely confirmed by using diplex DNA hybridization real PCR technique. Conclusion: Soft raw milk based cheese is a popular food in Egypt and looked on as a risk for foodborne bacteria contamination. The data of this study pointed out that there is a potential risk of infection with Listeria, especially the public health concern L. monocytogenes. The current study presented Vitek-2 compact system as advanced technique for not only for identification and differentiation of Listeria strains but also for their antimicrobial susceptibility. Furthermore the using of diplex real PCR technique gives a chance for quick and precise identification.

scholarly journals SURVEY ON PROTOTHECA SPP. OCCURRENCE IN RAW MILK FROM AUTOMATIC DISPENSER: PUBLIC HEALTH CONCERN

2011 ◽  
Vol 1 (1zero) ◽  
pp. 87
Author(s):  
G. Cammi ◽  
M. Merenda ◽  
F. Garilli ◽  
M. Ricchi ◽  
C. Garbarino ◽  
...  
Keyword(s):  
Public Health ◽  
Raw Milk ◽  
Health Concern ◽  
Public Health Concern

Antimicrobial Susceptibility and Virulence Characteristics of Salmonella enterica Typhimurium Isolates from Healthy and Diseased Pigs in Korea

2014 ◽  
Vol 77 (9) ◽  
pp. 1481-1486 ◽  
Author(s):  
MIGMA DORJI TAMANG ◽  
MAMATA GURUNG ◽  
HYANG-MI NAM ◽  
DONG CHAN MOON ◽  
GEUM-CHAN JANG ◽  
...  
Keyword(s):  
Public Health ◽  
Salmonella Typhimurium ◽  
Nalidixic Acid ◽  
Antimicrobial Susceptibility ◽  
Salmonella Enterica ◽  
Virulence Genes ◽  
Multidrug Resistant ◽  
Health Concern ◽  
Resistance Pattern ◽  
Public Health Concern

This study compared the antimicrobial susceptibility and prevalence of virulence genes in Salmonella enterica Typhimurium isolated from healthy and diseased pigs in Korea. A total of 456 Salmonella Typhimurium isolated from healthy (n = 238) and diseased (n = 218) pigs between 1998 and 2011 were investigated. In total, 93.4% of the Salmonella Typhimurium isolates were resistant to at least one antimicrobial agent tested. The isolates were most often resistant to tetracycline (85.7%), followed by streptomycin (83.6%), nalidixic acid (67.3%), ampicillin (49.3%), chloramphenicol (42.8%), and gentamicin (37.1%). Moreover, multidrug resistance phenotype and resistance to ampicillin, florfenicol, gentamicin, nalidixic acid, neomycin, streptomycin, and tetracycline were significantly higher (P < 0.01) among Salmonella Typhimurium isolates from the diseased pigs compared with those from the healthy pigs. The most common resistance pattern observed in both groups of isolates was streptomycin-tetracycline. Overall, more than 96% of the isolates tested possessed invA, spiA, msgA, sipB, prgH, spaN, tolC, lpfC, sifA, sitC, and sopB virulence genes. The prevalence of orgA, pagC, and iroN were 50.2, 74.1, and 91.0%, respectively, whereas isolates carrying cdtB (1.5%), pefA (7.0%), and spvB (14.9%) were identified much less frequently. Furthermore, the prevalence of invA, lpfC, orgA, pagC, and iroN was significantly higher (P < 0.01) among the isolates from the diseased pigs than in isolates from the healthy pigs. Our results demonstrated that, among diseased pigs, there was significantly higher resistance to some antimicrobials and greater prevalence of some virulence genes than in healthy pigs, indicating the role these factors play in pathogenesis. Multidrug-resistant Salmonella isolates that carry virulence-associated genes are potentially more dangerous and constitute a public health concern. Thus, continuous surveillance of antimicrobial resistance and virulence characteristics in Salmonella is essential.

scholarly journals Isolation and Molecular Characterization of Mycobacterium Africanum from the Sputum of Butchers in a Municipal Abattoir in

2021 ◽  
Vol 40 (4) ◽  
pp. 306-314
Author(s):  
C.A. Agada ◽  
I.F. Ijabone ◽  
D. Igwe ◽  
S.I.B. Cadmus
Keyword(s):  
Public Health ◽  
Molecular Characterization ◽  
Physical Contact ◽  
High Rate ◽  
Health Concern ◽  
Public Health Concern ◽  
Soft Cheese ◽  
Occupationally Exposed ◽  
Mycobacterium Africanum ◽  
High Level

Tuberculosis (TB) caused by the Mycobacterium tuberculosis complex (MTC) remains a major public health concern due to its high rate of person to person transfer as well as a high level of morbidity and mortality. The risk factors for transmission of zoonotic TB to humans are close physical contact with cattle, consumption of unpasteurised milk and milk products and unhealthy meat processing by butchers are common in developing countries like Nigeria. However, the circulating MTC among the occupationally exposed are unknown therefore the need to determine the prevalence of tuberculosis and to characterize the mycobacterial species in them. A crosssectional study was conducted among butchers, cattle traders and herders in Bodija Municipal Abattoir, Akinyele International Cattle Market and some herds respectively. Using systematic random sampling, 93 sputum samples were collected and analyzed by culture, Mycobacterium Genus Typing as well as Deletion Typing (Multiplex Polymerase Chain Reaction (PCR)). Of the 93 sputa collected, two (2.2%) were positive for mycobacteria by culture which were confirmed to be Mycobacterium africanum by molecular characterization. These bacilli were isolated from two butchers; one of which had the habit of eating raw meat and cherish ‘wara’ (a local soft cheese made from milk). The isolation of M. africanum from butchers in this study raises public health concern on the contamination of the meat processed as well as highlights its importance in the epidemiology of tuberculosis in Nigeria.

scholarly journals Antimicrobial susceptibility and MLVA analysis of S. Typhimurium strains isolated from human and poultry samples in Tunisia

2018 ◽  
Vol 12 (05) ◽  
pp. 313-320 ◽  
Author(s):  
Wissal Kalai ◽  
Ilargi Martinez ◽  
Joseba Bikandi ◽  
Lilia Messadi ◽  
Imed Khazri ◽  
...  
Keyword(s):  
Public Health ◽  
Antimicrobial Resistance ◽  
Salmonella Typhimurium ◽  
Antimicrobial Susceptibility ◽  
Multidrug Resistant ◽  
Health Concern ◽  
Processed Meat ◽  
Public Health Concern ◽  
Disk Diffusion Test ◽  
Antimicrobial Resistance Genes

Introduction: Salmonella enterica infections are a significant public health concern worldwide, being Salmonella Typhimurium one of the most prevalent serovars. Human salmonellosis is typically associated with the consumption of contaminated foods, such as poultry, eggs and processed meat. The extensive use of antimicrobials in humans and animals has led to an increase in multidrug resistance among Salmonella strains, becoming multidrug-resistant (MDR) strains a major public health concern. Methodology: This study was designed to investigate the antimicrobial susceptibility and the genotypic diversity of Salmonella Typhimurium strains isolated in Tunisia from human and poultry sources from 2009 to 2015. Fortyfive strains were analyzed by disk-diffusion test to determine the antimicrobial susceptibility. The presence of antimicrobial resistance genes was tested by PCR, and genotyping was performed using multiple-locus variable-number tandem repeats analysis (MLVA). Results: About 50% of the strains were resistant to at least 3 antibiotics (multidrug-resistant strains, MDR). The most frequent resistance profile in clinical strains was AMP-TIC-TET-MIN-SXT (n = 7) and TET-MIN in poultry origin strains (n = 7). The MLVA typing grouped the strains in 2 main clusters. Cluster I was mostly formed by human isolates, whereas in cluster II both human and poultry isolates were grouped. Simpson’s diversity index was 0.870 and 0.989 for antimicrobial resistance profiles and MLVA, respectively. Conclusions: Multiresistance is common in Salmonella Typhimurium isolated from human and poultry sources in Tunisia. The genotyping results suggest that some strains isolated from both sources may descend from a common subtype.

Reframing Fatness: Critiquing ‘Obesity’

2018 ◽  
Author(s):  
Bethan Evans ◽  
Charlotte Cooper
Keyword(s):  
Public Health ◽  
Weight Loss ◽  
Michel Foucault ◽  
Overweight And Obesity ◽  
Health Concern ◽  
Public Health Concern ◽  
Policy Language ◽  
Religious Imagery ◽  
Fat Bodies

Over the last twenty years or so, fatness, pathologised as overweight and obesity, has been a core public health concern around which has grown a lucrative international weight loss industry. Referred to as a ‘time bomb’ and ‘the terror within’, analogies of ‘war’ circulate around obesity, framing fatness as enemy.2 Religious imagery and cultural and moral ideologies inform medical, popular and policy language with the ‘sins’ of ‘gluttony’ and ‘sloth’, evoked to frame fat people as immoral at worst and unknowledgeable victims at best, and understandings of fatness intersect with gender, class, age, sexuality, disability and race to make some fat bodies more problematically fat than others. As Evans and Colls argue, drawing on Michel Foucault, a combination of medical and moral knowledges produces the powerful ‘obesity truths’ through which fatness is framed as universally abject and pathological. Dominant and medicalised discourses of fatness (as obesity) leave little room for alternative understandings.

European Surveillance of Antimicrobial Consumption: the ESAC programme

2004 ◽  
Vol 8 (32) ◽  
Author(s):  
Keyword(s):  
Public Health ◽  
Antimicrobial Resistance ◽  
Health Concern ◽  
Antimicrobial Consumption ◽  
Public Health Concern ◽  
Major Public Health Concern

Resistance to antimicrobials has become a major public health concern, and it has been shown that there is a relationship, albeit complex, between antimicrobial resistance and consumption

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