RAPID MULTIPLICATION OF MATURE FLOWERING PLANT OF Eryngium foetidum L. BY IN VITRO TECHNIQUE

Colocasia esculenta is commonly known as Taro, it is referred to as cocoyam in Nigeria. They are cherished for their rich taste, nutritional and medicinal properties. Every 100 g of taro corms possess 112 Kcal, 26.46 g carbohydrate, 1.50 g protein, 0.20 g total fat and 4.1g fiber (USDA National Nutrient Data Base). Besides its nutritional value, taro is used as a medical plant and provides bioactive compounds used as an anti-cancer drugs. Traditionally, cocoyams are vegetative propagated from tuber fragments, a practice that encourages pathogen distribution. Colocasia esculenta is a widely distributed food crop in the humid tropics and subtropics. Despite of its wide distribution, Taro plants are commonly infected with DsMV and other pathogens. This virus induces conspicuous mosaic, malformation, dwarfing or feathering on leaves in taro. As the results of infection, it reduces the quality and yield of taro production greatly. This virus is thus considered as a major limiting factor in the production of taro. Here plays the importance of tissue culture plays a major role in producing the disease resistant plants round the year with high quality. For rapid multiplication and production of quality planting materials, tissue culture technology offers promising alternative compared to the traditional production methods. KEYWORDS: Colocasia esculenta, Virus, Pathogens, Conventional propagation, Micropropagation, Yield, Rapid multiplication, Quality

Download Full-text

MICROPROPAGATION OF CHICKPEA FROM SHOOT TIP AND NODE SEGMENT

FLORA AND FAUNA ◽

10.33451/florafauna.v24i2pp270-274 ◽

2018 ◽

Vol 24 (2) ◽

Author(s):

J. D. BARSHILE

Keyword(s):

Shoot Tip ◽

Root Induction ◽

Multiple Response ◽

Ms Medium ◽

Growth Responses ◽

Rapid Multiplication ◽

Micro Propagation ◽

G 12 ◽

Better Than

Present investigation was undertaken to standardize technique for in vitro micro-propagation of chickpea( Cicer arietinum ) cultivar Vishwas (Phule G 12). Micropropagation method for chickpea was established and this method enabled much more efficient propagation of plants. The present work was aimed at evolving a protocol for rapid multiplication of chickpea using micropropagation technique. Explants from shoot tip and node segment were cultured on MS medium supplemented with different concentrations of BAP and Kinetin (1.0 to 2.5 mg/l) and their growth responses like shooting were elucidated. The maximum multiple response was observed with 2 mg/l concentration of BAP from both types of explant. The highest number of shoots (12.5 ± 0.3) was achieved on MS medium with 2 mg/l BAP using node segments. The medium supplemented with 2 mg/l of BAP was found better than all other concentrations. Individual shoots were transferred to IBA and IAA (1.0-1.5 mg/l) for root induction. MS medium supplemented with 2 mg/l of IBA proved better for rooting. Rooted plantlets were successfully hardened in greenhouse and established in the pot.

Download Full-text

Antitumor Potential of Lippia citriodora Essential Oil in Breast Tumor-Bearing Mice

Antioxidants ◽

10.3390/antiox10060875 ◽

2021 ◽

Vol 10 (6) ◽

pp. 875

Author(s):

Katerina Spyridopoulou ◽

Tamara Aravidou ◽

Evangeli Lampri ◽

Eleni Effraimidou ◽

Aglaia Pappa ◽

...

Keyword(s):

Breast Cancer ◽

Antiproliferative Activity ◽

Folk Medicine ◽

Tumor Model ◽

Flowering Plant ◽

Antitumor Effects ◽

Lippia Citriodora ◽

Antitumor Potential ◽

Apoptotic Marker

Lippia citriodora is a flowering plant cultivated for its lemon-scented leaves and used in folk medicine for the preparation of tea for the alleviation of symptoms of gastrointestinal disorders, cold, and asthma. The oil extracted from the plant leaves was shown to possess antioxidant potential and to exert antiproliferative activity against breast cancer. The aim of this study was to further investigate potential antitumor effects of L. citriodora oil (LCO) on breast cancer. The in vitro antiproliferative activity of LCO was examined against murine DA3 breast cancer cells by the sulforhodamine B assay. We further explored the LCO’s pro-apoptotic potential with the Annexin-PI method. The LCO’s anti-migratory effect was assessed by the wound-healing assay. LCO was found to inhibit the growth of DA3 cells in vitro, attenuate their migration, and induce apoptosis. Finally, oral administration of LCO for 14 days in mice inhibited by 55% the size of developing tumors in the DA3 murine tumor model. Noteworthy, in the tumor tissue of LCO-treated mice the apoptotic marker cleaved caspase-3 was elevated, while a reduced protein expression of survivin was observed. These results indicate that LCO, as a source of bioactive compounds, has a very interesting nutraceutical potential.

Download Full-text

Development of an in vitro technique for cytological investigations of slices of Fasciola hepatica: Evaluation by physiological criteria

International Journal for Parasitology ◽

10.1016/0020-7519(75)90081-8 ◽

1975 ◽

Vol 5 (3) ◽

pp. 333-337 ◽

Cited By ~ 8

Author(s):

L.T. Threadgold ◽

B. Hanna

Keyword(s):

Fasciola Hepatica ◽

In Vitro Technique

Download Full-text

Phospholipids in Cosmetics: In Vitro Technique Based on Phospholipids Membrane to Predict the in Vivo Effect of Surfactants

Phospholipids ◽

10.1007/978-1-4757-1364-0_8 ◽

1990 ◽

pp. 107-113

Author(s):

Gérard Redziniak

Keyword(s):

In Vitro Technique

Download Full-text

The standardization of a reproducible in vitro technique for the measurement of 86Rb uptake in the human erythrocyte

The International Journal of Applied Radiation and Isotopes ◽

10.1016/0020-708x(68)90123-3 ◽

1968 ◽

Vol 19 (11) ◽

pp. 781-788 ◽

Cited By ~ 1

Author(s):

Frank P. Castronovo ◽

Patricia A. McIntryre ◽

Howard S. Stern

Keyword(s):

Human Erythrocyte ◽

In Vitro Technique

Download Full-text

A Comparative Study of Antimicrobial Activity of Ethanolic Extract of Tecoma stans and Abutilon indicum Leaves: An In vitro Study

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i47b33161 ◽

2021 ◽

pp. 609-615

Author(s):

M. Binigha ◽

R. Gayatri Devi ◽

J. Selavaraj ◽

A. Jothi Priya

Keyword(s):

Antimicrobial Activity ◽

Agar Plate ◽

Ethanolic Extract ◽

Flowering Plant ◽

Diffusion Method ◽

Bacterial Strains ◽

Zone Of Inhibition ◽

The Family ◽

Abutilon Indicum

Tecoma stans is a flowering plant belonging to the family Bignoniaceae. It is the floral emblem of the Bahamas. Abutilon indicum belongs to the family Malvaceae. It is also called Indian Mallow. Tecoma stans and Abutilon indicumhave antimicrobial activity and can be used in producing antibiotics. There is an urgent need to produce new antibiotics as there is an increase in the development of pathogen resistant drugs. The aim of this study was to compare the antimicrobial activity of ethanolic extract of Tecoma stansand Abutilonindicum leaves. E.faecalis bacteria were maintained in nutrient agar slopes. The powder of the two herbs, were subjected to extraction by Soxhlet extractor and was concentrated to dry residue by Agar well diffusion method. The nutrient broth was inoculated with bacterial strains. The culture was adjusted to 0.5McFarland turbidity standard. Lawn culture was done in Muller-Hinton agar plate and then the plates were dried. A 6 mm diameter well is bored for different concentrations. The extracts were introduced into the well and allowed to stand for 24 hrs. The antibacterial activity was determined by measuring the zone of inhibition. The present finding on the antimicrobial potential of Abutilon indicum and Tecoma stands showed that the two plants have growth inhibitory activity against oral pathogen E. faecalis. Abutilon indicum has a zone of inhibition greater than Tecoma stans. Abutilon indicum exhibited better activity compared to Tecoma stans.

Download Full-text