Isolation and Characterization of a Biosurfactant Producing Strain Planococcus sp. XW-1 from the Cold Marine Environment

One cold-adapted strain, named Planococcus sp. XW-1, was isolated from the Yellow Sea. The strain can produce biosurfactant with petroleum as sole source of carbon at low temperature (4 °C). The biosurfactant was identified as glycolipid-type biosurfactant species by thin-layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR). It reduced the surface tension of water to 26.8 mN/m with a critical micelle concentration measurement of 60 mg/L. The produced biosurfactant possesses high surface activity at wide ranges of temperature (−18–105 °C), pH values (2–12), and salt concentrations (1–18%). The biosurfactant exhibited higher surface activity and higher growth rate of cells with hexadecane and diesel as carbon source. The strain Planococcus sp. XW-1 was also effective in degrading crude oil, after 21 days of growth at 4 °C in medium with 1% crude oil and 1% (v/v) bacteria broth, 54% of crude oil was degraded. The results suggest that Planococcus sp. XW-1 is a promising candidate for use in the bioremediation of petroleum-contaminated seawater in the Yellow Sea during winter. This study reported for the first time that Planococcus isolated from the Yellow Sea can produce biosurfactant using petroleum as the sole carbon source at low temperature (4 °C), showing its ecological role in the remediation of marine petroleum pollution.

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Isolation and Characterization of Crude Oil Degrading Bacteria in Association with Microalgae in Saver Pit from Egbaoma Flow Station, Niger Delta, Nigeria

Archives of Ecotoxicology ◽

10.36547/ae.2020.2.2.12-16 ◽

2020 ◽

Vol 2 (2) ◽

pp. 12-16

Author(s):

Obhioze Augustine Akpoka

Keyword(s):

Crude Oil ◽

Heterotrophic Bacteria ◽

Oil Pollution ◽

Heterotrophic Bacterium ◽

Bacterial Species ◽

Carbon Sources ◽

Sole Source ◽

Isolation And Characterization ◽

Degrading Bacteria ◽

Hydrocarbon Utilizing Bacteria

The capability of indigenous bacteria and microalgae in crude oil effluents to grow in and utilize crude oil as their sole source of carbon and energy provides an environmentally friendly and economical process for dealing with crude oil pollution and its inherent hazards. In view of the toxicity of crude oil spillages to indwellers of the affected ecosystems and the entire affected environment, the isolation of pure bacterial and microalgae cultures from crude effluents is a step in the right direction, particularly for bio-augmentation or bioremediation purposes. The total heterotrophic bacteria count and hydrocarbon utilizing bacteria count, as well as the microalgae count, were determined with the pour plate technique. The physicochemical properties of the effluent samples were also analyzed. Identification of the hydrocarbon utilizing bacteria was performed with phenotypic techniques. The result shows a mean total heterotrophic bacterium count of 5.91 log CFU/ml and a mean microalga count of 4.77 log cells/ml. When crude oil and polycyclic aromatic hydrocarbon (PAH) were used as sole carbon sources, total hydrocarbon utilizing bacteria counts were respectively estimated at 3.89 and 2.89 log CFU/ml. Phenotypic identification of hydrocarbon utilizing bacteria in the crude oil effluents revealed the presence of two main bacterial genera: Streptococcus and Pseudomonas. Data obtained from this study confirmed the biodegradative abilities of indigenous bacterial species, thus, ultimately resulting in the amelioration of the toxicity associated with the crude oil effluents.

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Surface activity of salt-tolerant Serratia spp. and crude oil biodegradation in saline soil

Plant Soil and Environment ◽

10.17221/217/2012-pse ◽

2012 ◽

Vol 58 (No. 9) ◽

pp. 412-416 ◽

Cited By ~ 14

Author(s):

T. Wu ◽

W.J. Xie ◽

Y.L. Yi ◽

X.B. Li ◽

H.J. Yang ◽

...

Keyword(s):

Surface Tension ◽

Salt Tolerance ◽

Crude Oil ◽

Surface Activity ◽

High Surface ◽

Saline Soils ◽

Surface Tension Reduction ◽

Oil Biodegradation ◽

Soil Solutions ◽

Nacl Concentration

An ideal strain for crude oil degradation in saline soils would be one with high salt-tolerance. A novel bacterial strain, Serratia sp. BF40, was isolated from crude oil contaminated saline soils. Its salt-tolerance, surface activity and ability to degrade crude oil in saline soils were evaluated. It can grow in liquid culture with NaCl concentration less than 6.0%. Its surface activity characterized as an efficient surface tension reduction, was significantly affected by salinity above 2.0%. BF40 inoculation could decrease surface tension of soil solutions and facilitate crude oil removal in soils with 0.22–1.20% salinity, but the efficiency was both significantly lower than its biosurfactant addition. The BF40 strain has a high potential for biodegradation of crude oil contaminated saline soils in view of its high surface activity and salt-tolerance, which is the first report of biosurfactant producing by the genus Serratia for petroleum degrading. We suggest that biosurfactant addition is an efficient strategy. Simultaneously, the growing status of the strain and how to boost its surface activity in saline soils should deserve further studies in order to achieve a continuous biosurfactant supply.

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Redescription of three species of Hysterothylacium (Nematoda: Anisakidae) from marine fishes from the Yellow Sea, China, with the synonymy of Hysterothylacium muraenesoxin (Luo, 1999)

Zootaxa ◽

10.11646/zootaxa.1878.1.2 ◽

2008 ◽

Vol 1878 (1) ◽

pp. 55 ◽

Cited By ~ 15

Author(s):

LIANG LI ◽

ZHEN XU ◽

LUPING ZHANG

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Yellow Sea ◽

Taiwan Strait ◽

Marine Fishes ◽

The Yellow Sea ◽

Trachurus Japonicus ◽

First Time ◽

Scanning Electron

Three species of Hysterothylacium Ward & Magath, 1917 from the Yellow Sea, China, are redescribed and illustrated by light and scanning electron microscopy. Hysterothylacium fabri (Rudolphi, 1819) collected from Chelidonichthys kumu (Cuvier), Trachurus japonicus (Temminck & Schlegel), Argyrosomus argentatus (Houttuyn), Astroconger myriaster (Brevoort) and Hysterothylacium tasmaniense (Johnston & Mawson, 1945) collected from Lophius litulon (Jordan) are reported for the first time in China. Hysterothylacium amoyense (Hsü, 1933) (originally registered as Contracaecum amoyensis from Amoy, Taiwan Strait, China) is redescribed and compared with Hysterothylacium muraenesoxin (Luo, 1999), concluding that the second species represents a junior synonymy of H. amoyense.

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Isolation and Characterization of Antiangiogenesis Compounds from the Fungus Aspergillus terreus Associated with Apostichopus japonicus Using Zebrafish Assay

Natural Product Communications ◽

10.1177/1934578x1701200231 ◽

2017 ◽

Vol 12 (2) ◽

pp. 1934578X1701200 ◽

Cited By ~ 1

Author(s):

Jun Qi ◽

Bo Zhao ◽

Peipei Zhao ◽

Airong Jia ◽

Yonggang Zhang ◽

...

Keyword(s):

Spectral Methods ◽

Yellow Sea ◽

Aspergillus Terreus ◽

Apostichopus Japonicus ◽

The Yellow Sea ◽

First Report ◽

Isolation And Characterization

Three compouds, (+)-butyrolactone IV (1), butyrolactone I (2) and terrelactone A (3) were isolated from the fungus Aspergillus terreus associated with Apostichopus japonicus from the Yellow Sea in China; their structures were elucidated by spectral methods. Compounds 1 and 2 were shown to have moderate antiangiogenesis activity when tested using the zebrafish assay. This is the first report of butyrolactones with antiangiogenesis activity.

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The genus Eudorella (Crustacea: Cumacea) from the Yellow Sea, with description of a new species

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s0025315498000575 ◽

1999 ◽

Vol 79 (3) ◽

pp. 445-457

Author(s):

Jae-Sang Hong ◽

Mi-Ra Park

Keyword(s):

New Species ◽

Yellow Sea ◽

Cold Water ◽

The Yellow Sea ◽

Shallow Waters ◽

Korean Waters ◽

Sea Bottom ◽

First Time ◽

Bottom Cold Water ◽

A New Species

Two species of the genus Eudorella (Crustacea: Cumacea) were collected and examined from the Yellow Sea. A new species and one new record of Eudorella are described and illustrated. This new species Eudorella hwanghaensis sp. nov. closely resembles E. intermedia, but it differs from the latter species in bearing a distinct apical spine of endopod with uropod peduncle. The distribution of the new species is confined to the central portion of the Yellow Sea, and its distributional range coincides well with that of the Yellow Sea Bottom Cold Water Mass. Eudorella pacifica is recorded for the first time in Korean waters. It occurred in shallow waters and was distributed widely on both coastal sides of the Yellow Sea.

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Summer reproduction of the planktonic copepod Calanus sinicus in the Yellow Sea: influences of high surface temperature and cold bottom water

Journal of Plankton Research ◽

10.1093/plankt/fbm005 ◽

2007 ◽

Vol 29 (2) ◽

pp. 179-186 ◽

Cited By ~ 25

Author(s):

Guang-Tao Zhang ◽

Song Sun ◽

Bo Yang

Keyword(s):

Surface Temperature ◽

Yellow Sea ◽

Bottom Water ◽

High Surface ◽

The Yellow Sea ◽

Calanus Sinicus ◽

Planktonic Copepod ◽

High Surface Temperature

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Structure identification of Ti(iv) clusters in low-temperature TiO2 crystallization: creating high-surface area brush-shaped rutile TiO2

CrystEngComm ◽

10.1039/c7ce00928c ◽

2017 ◽

Vol 19 (39) ◽

pp. 5844-5848 ◽

Cited By ~ 3

Author(s):

Kazuhiro Manseki ◽

Kazuki Saka ◽

Masaki Matsui ◽

Saeid Vafaei ◽

Takashi Sugiura

Keyword(s):

Low Temperature ◽

Surface Area ◽

High Surface ◽

High Surface Area ◽

Large Surface Area ◽

Structure Identification ◽

Rutile Tio2 ◽

First Time

We demonstrate for the first time a controlled reaction of Cl-coordinating Ti(iv) clusters in low-temperature hydrolysis (27 °C) to create large-surface area rutile TiO2 crystals.

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Draft Genome Sequence of the Crude Oil-Degrading and Biosurfactant-Producing StrainCobetiasp. QF-1

Genome Announcements ◽

10.1128/genomea.01456-17 ◽

2018 ◽

Vol 6 (3) ◽

Cited By ~ 1

Author(s):

Ping Guo ◽

Binxia Cao ◽

Xue Qiu ◽

Jianguo Lin

Keyword(s):

Low Temperature ◽

Crude Oil ◽

Draft Genome ◽

The Yellow Sea ◽

Oil Degradation ◽

Protein Coding ◽

Coding Sequences ◽

Cold Adapted ◽

Crude Oil Degradation ◽

Production Activity

ABSTRACTWe report here the draft genome ofCobetiasp. QF-1, a cold-adapted bacterium isolated from crude oil-contaminated seawater of the Yellow Sea, China. This genome is approximately 4.1 Mb (G+C content, 57.44%) with 3,513 protein-coding sequences.Cobetiasp. QF-1 shows crude oil degradation and biosurfactant production activity at low temperature.

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Isolation and Characterization of a new Benzofuran from the Fungus Alternaria sp. (HS-3) Associated with a Sea Cucumber

Natural Product Communications ◽

10.1177/1934578x1100601230 ◽

2011 ◽

Vol 6 (12) ◽

pp. 1934578X1100601 ◽

Cited By ~ 1

Author(s):

Xuekui Xia ◽

Jun Qi ◽

Fang Wei ◽

Airong Jia ◽

Wenpeng Yuan ◽

...

Keyword(s):

Spectral Methods ◽

Yellow Sea ◽

Sea Cucumber ◽

The Yellow Sea ◽

Isolation And Characterization ◽

Alternaria Sp

A new compound, 4-acetyl-5-hydroxy-3, 6, 7-trimethylbenzofuran-2(3H)-one (1), together with two known compounds, 2-carboxy-3-(2-hydroxypropanyl) phenol (2) and 5-methyl- 6-hydroxy-8-methyoxy-3-methylisochroman (3) were isolated from the fungus Alternaria sp. (HS-3) associated with a sea cucumber from the Yellow Sea in China. Their structures were elucidated by spectral methods.

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