scholarly journals In Vitro Antiviral Activity of Green Tea Polyphenon-60 against Avian Paramyxoviruses

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Hang Minh Pham
Keyword(s):  
Green Tea ◽  
Chicken Embryo ◽  
Antiviral Agent ◽  
Inhibitory Effect ◽  
High Morbidity ◽  
Poultry Production ◽  
Significant Drop ◽  
Hemagglutination Inhibition Test ◽  
Safety Margins

Avian paramyxoviruses (APMVs) have caused an economically significant drop in global domestic poultry production because of their high morbidity and mortality rates. Polyphenols are the major components of green tea that have great antiviral effects. This study aimed to evaluate the anti-APMV activities of polyphenon-60. Twelve APMV-1 strains representing three different pathotypes, two strains of APMV-2, one strain of APMV-3, and one strain of APMV-7 were propagated in chicken embryos. To determine the cytotoxic effect, chicken embryo fibroblasts were treated with the test compound in various concentrations. To assess the antiviral properties, time-dependent, dose-dependent, and virulence-dependent experiments were conducted in both cell and chicken embryo models. A reduction in virus titers was measured by the hemagglutination test. The inhibitory effect on virus adsorption to the chicken red blood cell (RBC) surface was examined by the hemagglutination inhibition test. The results showed that lentogenic and mesogenic APMV-1 strains, APMV-3 strain, and APMV-7 strain were significantly inhibited ( p < 0.05 ) by polyphenon-60 at 50 μg/ml, while the 50% cytotoxic concentration of the compound was 345 μg/ml. Polyphenon-60 also exhibited the inhibitory activity against hemagglutination by NDV. Taken together, the results suggest that polyphenon-60 has shown promise as an antiviral agent that has wide safety margins against APMVs, and challenge studies to evaluate its efficacy in chickens are necessary.

scholarly journals In Vitro Growth- and Encystation-Inhibitory Efficacies of Matcha Green Tea and Epigallocatechin Gallate Against Acanthameoba Castellanii

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 763
Author(s):  
Ameliya Dickson ◽  
Elise Cooper ◽  
Lenu B. Fakae ◽  
Bo Wang ◽  
Ka Lung Andrew Chan ◽  
...  
Keyword(s):  
Green Tea ◽  
Colorimetric Assay ◽  
Epigallocatechin Gallate ◽  
Acanthamoeba Castellanii ◽  
Inhibitory Effect ◽  
Response To Treatment ◽  
Ftir Microscopy ◽  
Sulforhodamine B ◽  
Chemical Fingerprinting

We examined the inhibitory effect of matcha green tea (Camellia sinensis) and epigallocatechin gallate (EGCg; the most abundant catechin in tea) on the vegetative growth and encystation of Acanthamoeba castellanii T4 genotype. The sulforhodamine B (SRB) stain-based colorimetric assay and hemocytometer counting were used to determine the reduction in A. castellanii trophozoite proliferation and encystation, in response to treatment with C. sinensis or EGCg. Fourier transform infrared (FTIR) microscopy was used to analyze chemical changes in the trophozoites and cysts due to C. sinensis treatment. Hot brewed and cold brewed matcha inhibited the growth of trophozoites by >40% at a 100 % concentration. EGCg at concentrations of 50 to 500 µM significantly inhibited the trophozoite growth compared to control. Hot brewed matcha (100% concentration) also showed an 87% reduction in the rate of encystation compared to untreated control. Although 500 µM of EGCg increased the rate of encystation by 36.3%, 1000 µM reduced it by 27.7%. Both percentages were not significant compared to control. C. sinensis induced more cytotoxicity to Madin Darby canine kidney cells compared to EGCg. FTIR chemical fingerprinting analysis showed that treatment with brewed matcha significantly increased the levels of glycogen and carbohydrate in trophozoites and cysts.

Inhibitory Effect of (−)-Epigallocatechin 3-Gallate, a Polyphenol of Green Tea, on Neutrophil Chemotaxis in Vitro and in Vivo

2004 ◽  
Vol 52 (14) ◽  
pp. 4571-4576 ◽  
Author(s):  
Katsuhiko Takano ◽  
Keiko Nakaima ◽  
Makoto Nitta ◽  
Futoshi Shibata ◽  
Hideo Nakagawa
Keyword(s):  
Green Tea ◽  
Inhibitory Effect ◽  
Neutrophil Chemotaxis

Inhibitory effects of (-)-Epigallocatechin-3-gallate from green tea on the growth of Babesia parasites

Parasitology ◽  
2009 ◽  
Vol 137 (5) ◽  
pp. 785-791 ◽  
Author(s):  
M. ABOULAILA ◽  
N. YOKOYAMA ◽  
I. IGARASHI
Keyword(s):  
Body Weight ◽  
Green Tea ◽  
Inhibitory Effect ◽  
Post Inoculation ◽  
Chemotherapeutic Drug ◽  
Inhibitory Effects ◽  
Viability Test ◽  
Anti Cancer

SUMMARY(-)-Epigallocatechin-3-gallate (EGCG) is the major tea catechin and accounts for 50–80% of the total catechin in green tea. (-)-Epigallocatechin-3-gallate has antioxidant, anti-inflammatory, anti-microbial, anti-cancer, and anti-trypanocidal activities. This report describes the inhibitory effect of (-)-Epigallocatechin-3-gallate on the in vitro growth of bovine Babesia parasites and the in vivo growth of the mouse-adapted rodent babesia B. microti. The in vitro growth of the Babesia species was significantly (P<0·05) inhibited in the presence of micromolar concentrations of EGCG (IC50 values=18 and 25 μM for B. bovis, and B. bigemina, respectively). The parasites showed no re-growth at 25 μM for B. bovis and B. bigemina in the subsequent viability test. The drug significantly (P<0·05) inhibited the growth of B. microti at doses of 5 and 10 mg/kg body weight, and the parasites completely cleared on day 14 and 16 post-inoculation in the 5 and 10 mg/kg treated groups, respectively. These findings highlight the potentiality of (-)-Epigallocatechin-3-gallate as a chemotherapeutic drug for the treatment of babesiosis.

scholarly journals Comparison of Inhibitory Effect of Green Tea Extract and Nystatin on Candida Albicans Growth: An in-vitro Study

2020 ◽  
Vol 17 (4) ◽  
pp. 287-295
Author(s):  
lida samie ◽  
zeynab bayat ◽  
dara dastan ◽  
mohammad yusef alikhani ◽  
jalal poorolajal ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Green Tea ◽  
In Vitro Study ◽  
Inhibitory Effect ◽  
Green Tea Extract ◽  
Vitro Study ◽  
Tea Extract

scholarly journals Losartan promotes cell survival following SARS-CoV-2 infection in vitro

2020 ◽  
Author(s):  
Reza Nejat ◽  
Ahmad Shahir Sadr ◽  
Brendan T. Freitas ◽  
Jackelyn Crabtree ◽  
Scott D. Pegan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Angiotensin Ii ◽  
Post Infection ◽  
Inhibitory Effect ◽  
High Morbidity ◽  
Type I ◽  
Infection Group ◽  
Shift Analysis

AbstractIntroductionCoronavirus disease 2019 (COVID-19) can be associated with mortality and high morbidity worldwide. There is an extensive effort to control infection and disease caused by SARS-CoV-2. This study addressed the hypothesis that angiotensin II type I receptor blocker, Losartan, may restrict pathogenesis caused by SARS-CoV-2 by decreasing viral-induced cytopathological changes by blocking angiotensin II type 1 receptor (AT1R), thus reducing the affinity of the virus for ACE2, and inhibiting papain-like protease of the virus.MethodLosartan inhibitory effect on deubiquitination and deISGylation properties of papain-like protease was investigated using a fluorescence method and gel shift analysis determining its inhibitory effects.The inhibitory effect of Losartan on SARS-CoV-2 cell replication was investigated both when losartan was added to the cell culture 1 hour before (pre-infection group) and 1 hour after (post-infection group) SARS-CoV-2 infection of Vero E6 cells.ResultsLosartan treatment of Vero E6 cells prior to and after SARS-CoV-2 infection reduced SARS-CoV-2 replication by 80% and 70% respectively. Losartan was not a strong deubiquitinase and deISGylase inhibitor of PLpro.ConclusionLosartan added pre- and post-infection to the Vero E6 cell culture significantly prevents cell destruction and replication by SARS-CoV2. Losartan has low side-effects, is readily available, and can be produced at high levels globally, all features of a promising drug in treatment of COVID-19 if validated by clinical trials.

scholarly journals In-Vitro Inhibition of Staphylococcal Pathogenesis by Witch-Hazel and Green Tea Extracts

Antibiotics ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 244 ◽  
Author(s):  
Reuven Rasooly ◽  
Adel Molnar ◽  
Hwang-Yong Choi ◽  
Paula Do ◽  
Kenneth Racicot ◽  
...  
Keyword(s):  
Green Tea ◽  
Toxin Production ◽  
The United States ◽  
Inhibitory Effect ◽  
Biofilm Inhibition ◽  
Witch Hazel ◽  
Antimicrobial Effects ◽  
The Witch ◽  
Biofilm Development

whISOBAX (WH), an extract of the witch-hazel plant that is native to the Northeast coast of the United States, contains significant amounts of a phenolic compound, Hamamelitannin (HAMA). Green tea (GT) is a widely consumed plant that contains various catechins. Both plants have been associated with antimicrobial effects. In this study we test the effects of these two plant extracts on the pathogenesis of staphylococci, and evaluate their effects on bacterial growth, biofilm formation, and toxin production. Our observations show that both extracts have antimicrobial effects against both strains of S. aureus and S. epidermidis tested, and that this inhibitory effect is synergistic. Also, we confirmed that this inhibitory effect does not depend on HAMA, but rather on other phenolic compounds present in WH and GT. In terms of biofilm inhibition, only WH exhibited an effect and the observed anti-biofilm effect was HAMA-depended. Finally, among the tested extracts, only WH exhibited an effect against Staphylococcal Enterotoxin A (SEA) production and this effect correlated to the HAMA present in WH. Our results suggest that GT and WH in combination can enhance the antimicrobial effects against staphylococci. However, only WH can control biofilm development and SEA production, due to the presence of HAMA. This study provides the initial rationale for the development of natural antimicrobials, to protect from staphylococcal colonization, infection, or contamination.

scholarly journals Uji Aktivitas Antidiabetes Ekstrak Teh Hitam dan Teh Hijau secara In Vitro Menggunakan Metode Inhibisi Enzim α-Glukosidase

2018 ◽  
Vol 6 (2) ◽  
pp. 235
Author(s):  
Diana Holidah ◽  
Yasmin Yasmin ◽  
Fransiska Maria Christianty
Keyword(s):  
Diabetes Mellitus ◽  
Green Tea ◽  
Metabolic Diseases ◽  
Black Tea ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Antidiabetic Activity ◽  
Increase Blood Glucose Level ◽  
Tea Extract

  Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia. Carbohydrates are metabolized into glucose, in gastrointestinal tract than absorbed into the bloodstream and increase blood glucose level. This absorption process is catalyzed by α-glucosidase enzyme. Previous studies showed that tea (Camellia sinensis) extract have antidiabetic activity in mice induced by streptozotocin. The aim of this study was to compared the in vitro activity of tea extract in inhibiting α-glucosidase. Black tea and green trea extracts were tested for α-glucosidase inhibitory activity. Acarbose was used as positive control. IC50 extract was 54.86 µg/mL for black tea and 44.79 µg/mL for green tea. This study showed that inhibitory effect of green tea was higher than black tea.   Keywords: black tea, green tea, diabetes mellitus, α-glucosidase  

scholarly journals Characterization of the Trans-Epithelial Transport of Green Tea (C. sinensis) Catechin Extracts with In Vitro Inhibitory Effect against the SARS-CoV-2 Papain-like Protease Activity

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6744
Author(s):  
Carmela Maria Montone ◽  
Sara Elsa Aita ◽  
Anna Arnoldi ◽  
Anna Laura Capriotti ◽  
Chiara Cavaliere ◽  
...  
Keyword(s):  
Green Tea ◽  
Crude Extract ◽  
Analytical Approach ◽  
Epithelial Transport ◽  
Synergistic Effects ◽  
Inhibitory Effect ◽  
Ic50 Value ◽  
Identification And Characterization

This work describes an untargeted analytical approach for the screening, identification, and characterization of the trans-epithelial transport of green tea (Camellia sinensis) catechin extracts with in vitro inhibitory effect against the SARS-CoV-2 papain-like protease (PLpro) activity. After specific catechin extraction, a chromatographic separation obtained six fractions were carried out. The fractions were assessed in vitro against the PLpro target. Fraction 5 showed the highest inhibitory activity against the SARS-CoV-2 PLpro (IC50 of 0.125 μg mL−1). The untargeted characterization revealed that (−)-epicatechin-3-gallate (ECG) was the most abundant compound in the fraction and the primary molecule absorbed by differentiated Caco-2 cells. Results indicated that fraction 5 was approximately 10 times more active than ECG (IC50 value equal to 11.62 ± 0.47 μg mL−1) to inhibit the PLpro target. Overall, our findings highlight the synergistic effects of the various components of the crude extract compared to isolated ECG.

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