CNT-Based Nano Medicine From Synthesis to Therapeutic Application

Carbon nanotubes (CNTs) are allotropes of carbon consisting of cylindrical tubes, made up of graphite with a diameter of several nm to a length of several mm. They had extraordinary structural, mechanical, and electronic properties due to their small size and mass, high mechanical resilience, and high electrical and thermal conductivity. Their large surface area made them applicable in pharmacy and medicine and adsorb or conjugate a broad variety of medical and diagnostic agents (drugs, genes, vaccines, antibodies, biosensors, etc.). They are often used to deliver drugs directly into the cells without going through the metabolic process of body. In addition to drug delivery and gene therapy, CNTs are also used for tissue regeneration, diagnostic biosensors, chiral drug enantiomer separation, drug extraction, and drug or pollutant analysis. CNTs have recently been discovered as effective antioxidants. The ADME and toxicity of different types of CNTs have also been documented here, as well as the prospects, advantages, and challenges of this promising bio-nano technology.

Diabetes and Nanotechnology –A recent advance in treatment of Diabetes

The combination of nanotechnology and medicine has created a new field “Nano medicine” to enhance human health care. Nanotechnology can provide sensing technologies for accurate and medical information, for diagnosis of diabetes. The aim of this review is to provide insights into the role of nanotechnology in diabetes diagnosis and treatment, shedding light on the potential of nanotechnology in this field and discussing the future prospects. Natural polymers are essential to daily life as our human forms are based on them. Natural products are considered as a major source of medicaments and, hence, they are extensively used by pharmaceutical industries.

Standardization, Characterization and Isolation of Trichoderma-Silver Nanoparticle-A Pharmaceutical Approach in Field of Nano-Medicine

Silver is a well known antimicrobial agent. It is utilized in many antimicrobial and medication. The Trichoderma and its Metabolites have also been meant for antimicrobial activity against the various bacterial and fungal strain. In the present investigation Nanoparticle of silver is prepared by chemical method, green synthesis using plants and biosynthesis using microbes. Trichoderma secondary metabolite prepared by solvent extraction method from Trichoderma harzianum which act as a capping and reducing agent. The biosynthesised silver Nanoparticles were characterized by UV-Vis spectroscopy and TEM. UV-Vis spectra of silver Nanoparticle and trichoderma extract showed absorption spectra at 420nm & 430 nm respectively while the fused nanoparticle with Trichoderma secondary metabolite showed absorption spectra at 415 nm corresponding to the surface Plasmon resonance of silver Nanoparticle. It was determined the nanoparticle showed absorption spectra at 415 nm and morphology as spherical with size range 8 to 24 nm and providing good antimicrobial activity as Trichoderma silver fused nanoparticle against many microbial strain, so it can be prepared for pharmaceutical approach against the infectious disease caused by clinical pathogenic organisms.

Mannose Modified Co-Loaded Zoledronic Liposomes Deplete M2-tumor-associated Macrophages to Enhance Anti-Tumor Effect of Doxorubicin on TNBC

As the most numerous inflammatory cell group in tumor microenvironment, tumor-associated macrophages (TAMs) have an essential effect in tumor therapy and are potential therapeutic targets. M2 type tumor-associated macrophages (M2-TAMs) were involved in the entire process of tumor development, invasion, and metastasis, obstructing the anti-tumor effect of chemotherapy drugs and nano-medicine. This study aimed to construct a mannose modified co-loaded zoledronic acid and doxorubicin liposomes (Man-LP@ZOL/DOX) for improving the anti-tumor effect by suppressing M2-TAMs on triple-negative breast cancer (TNBC). The size, PDI, and Zeta-potential of Man-LP@ZOL/DOX liposomes were determined to be 212.80±7.74 nm, 0.1413±0.0232, -33.63±0.49 mV, respectively. The Man-LP@ZOL/DOX's EE% and DL% of ZOL as measured were 17.21±2.26% and 1.56±0.21%, while for DOX, they were 84.42±2.05% and 5.12±0.14%. The uptake of Man-LP into cells was increased when it was modified with a TAMs target ligand. The liposomes inhibited the invasion of MDA-MB-231 cells induced by M2-TAMs, and expression of biomarkers on M2-TAMs (Arg1 and CD206 in vitro, CD68, and CD206 in vivo) was apparent. Moreover, co-loaded drugs liposome system remarkably enhanced anticancer effects both in vitro and vivo combined with ZOL. In conclusion, all these results established that Man-LP@ZOL/DOX could enhance anti-tumor effect of DOX via depleting M2-TAMs on TNBC.

Liposomes as Drug Delivery System: An Updated Review

The liposomes were the first Nano medicine to be accepted for clinical use. They are the spherical vesicles that possess mid empty aqueous space, which is encircled by a phospholipids bilayer. Liposomes have immense capability to prevent the degradation of drugs, reduce side effects and are thus increasingly used for targeted drug delivery. The drugs can either be incorporated inside the aqueous space (hydrophilic drugs) or inside the phospholipids bilayer (hydrophobic drugs) of liposomes for the targeted drug delivery. Considering the importance of liposomes as a drug delivery system, the present review paper tries to look into its details. The entire paper is classified into six parts. The first part is introductory. The second part discusses the classification of liposomes. In the third segment, the structural components of liposomes are detailed. The fourth portion of the paper talks about methods of preparation of liposomes. In the fifth segment, the characterization of liposomes is discussed. The sixth part discusses the application of liposomes and the last part is given to concluding observation. Literature shows distinct types of liposomes, categorized based on size, number of lipid layers, composition and preparation method. They are recently used for various nanoscale drugs formulation and a piece of concrete evidence was seen recently in recommended drug for black fungus i.e., Liposomal Amphotericin B. Although, their development and application are remaining the challenge due to costly and tedious processes involved in their production and development. Therefore, further research and development are required to perform to overcomes these challenges. Keywords: Liposome, characterization, amphiphatic, controlled release, phospholipids

Brief Review on Future of Medicine: Nanorobots

Nano-robots are the robots that are the technology of creating machines or robots close to the microscopic scale of a nanometre (10-9meters). These devices range from 0.1 – 10 micrometres. Nano-robots are the advances in technology. The technology has expanded our capability and potential to operate the world around us on above removing scale. Nano robots can be used in various application such as space and medicine technology. Nanos robots are device is used for motive of nourishing, keeping and protecting the human body opposing antibody, microorganisms, pathogens. Nano robots are instrumental by using various components such as actuators, sensors, control, power, conveying and by affiliating cross – marked scales between organic and inorganic systems. Nanotechnologies are quickly arising within the nation of medicine, and this subdivision has been termed Nano medicine. Application for nanorobotics in medicine including diagnosis and drug delivery for cancer, tissue engineering, pharmacokinetics, dental surgery, ophthalmology and others. This region of work is fulfilled with prospective and possible applications, many of like that recently animating researched and evolution. The objective of this paper is to introduce the expanding sector of nanorobotics inside in medicine and advancement in medical application.

Tailoring of plasmonic functionalized metastructures to enhance local heating release

Plasmonic nanoheaters are reported that produce a significant local heating when excited by a 532 nm wavelength focused laser beam. A significant temperature increase derives from the strong confinement of electric field enabled by the specific arrangement of Au nanodisks constituting the nanoheater. The thermal response is much more sensitive when layering the gold nanoheaters by a thick layer of doped polymer, reaching a temperature variation of more than 250 °C. The modulation of the excitation by a chopper enables the fine control of the thermal response with a measured maximum temperature variation of about 60 °C in a single period. These intriguing features can be efficiently exploited for the design of novel systems finding application in nano medicine and nano chemistry.

Role of structural specificity of ZnO particles in preserving functionality of proteins in their corona

Reconfiguration of protein conformation in a micro and nano particle (MNP) protein corona due to interaction is an often-overlooked aspect in drug design and nano-medicine. Mostly, MNP-Protein corona studies focus on the toxicity of nano particles (NPs) in a biological environment to analyze biocompatibility. However, preserving functional specificity of proteins in an NP corona becomes critical for effective translation of nano-medicine. This paper investigates the non-classical interaction between insulin and ZnO MNPs using a classical electrical characterization technique at GHz frequency with an objective to understand the effect of the micro particle (MP) and nanoparticle (NP) morphology on the electrical characteristics of the MNP-Protein corona and therefore the conformation and functional specificity of protein. The MNP-Protein corona was subjected to thermal and enzymatic (papain) perturbation to study the denaturation of the protein. Experimental results demonstrate that the morphology of ZnO particles plays an important role in preserving the electrical characteristics of insulin.

Mesenchymal Stem Cell-Derived Exosomes as an Emerging Paradigm for Regenerative Therapy and Nano-Medicine: A Comprehensive Review

Mesenchymal Stem Cells are potent therapeutic candidates in the field of regenerative medicine, owing to their immunomodulatory and differentiation potential. However, several complications come with their translational application like viability, duration, and degree of expansion, long-term storage, and high maintenance cost. Therefore, drawbacks of cell-based therapy can be overcome by a novel therapeutic modality emerging in translational research and application, i.e., exosomes. These small vesicles derived from mesenchymal stem cells are emerging as new avenues in the field of nano-medicine. These nano-vesicles have caught the attention of researchers with their potency as regenerative medicine both in nanotherapeutics and drug delivery systems. In this review, we discuss the current knowledge in the biology and handling of exosomes, with their limitations and future applications. Additionally, we highlight current perspectives that primarily focus on their effect on various diseases and their potential as a drug delivery vehicle.