A new Cnemaspis Strauch, 1887 (Squamata: Gekkonidae) from the northern Western Ghats, Maharashtra, India

A new species belonging to the genus Cnemaspis Strauch, 1887 is described from the Rangana Fort mountain range of the northern Western Ghats, Maharashtra, India. A preliminary 16S rRNA phylogeny of Indian Cnemaspis is provided and the phylogenetic position of the new species is established within the goaensis clade. The new species, Cnemaspis ranganaensis sp. nov. can easily be distinguished from all Indian congeners by having 3 or 4 spine-like tubercles on the flanks, conical tubercles absent on flank, dorsal pholidosis heterogeneous, presence of precloacal and femoral pores in males, 8 or 9 poreless scales between femoral and precloacal pores; gular scales flat, smooth; 93–101 paravertebral scales, 59–63 mid-dorsal scales; 93–101 longitudinal scales from mental to cloaca, 30–31 scales across belly; ventral scales smooth, imbricate; tail with small, granular, keeled, pointed, subimbricate scales intermixed with enlarged, strongly keeled, conical tubercles forming whorls; scales on ventral side of tail imbricate, smooth, with three rows of large, slightly elongated median subcaudals. The new species is closely related to C. goaensis, from which it differs by a genetic divergence of 1.9-3.0%. Molecular phylogenetic reconstruction supports the distinctiveness of the new species.

Description of Stenomitos kolaenensis and S. hiloensis sp. nov. (Leptolyngbyaceae, Cyanobacteria) with an emendation of the genus

Stenomitos is a recently established cyanobacterial genus, some species of which appear to be cryptic. Here we describe two new species in this genus, Stenomitos kolaensis sp. nov. isolated from the Al-Fe humic podzols of a boreal forest near Nikel town, Murmansk region, Russia and S. hiloensis sp. nov. isolated from a basaltic seep wall on Akeola Road, Hilo, Hawaii, USA. Phylogenetic analyses were conducted on the 16S and 16S-23S ITS rRNA gene regions using Bayesian Inference, and Maximum Likelihood. Phylogenetic analysis of the 16S-23S ITS rRNA region resulted in both S. kolaensis and S. hiloensis forming separate clades from other Stenomitos lineages. Antarctic strains of Stenomitos frigidus (previously reported as “Leptolyngbya frigida”) show that species to be polyphyletic and in need of revision. The structure of the conserved ITS regions (Box-B, D1-D1ʹ, V2 and V3 helices) provided support for separation of the species, and the p-distances among aligned ITS regions further confirmed that a number of species exist within the genus. S. kolaensis and S. hiloensis can be distinguished from other described Stenomitos species (S. rutilans and S. tremulus) by their geographical distribution, habitat preference, 16S rRNA phylogeny, and differences in the secondary structure of the 16S-23S ITS region.

Genome Sequence of the Marine Alphaproteobacterium HTCC2150, Assigned to the Roseobacter Clade

ABSTRACT Here we announce the genome sequence of a marine bacterium, HTCC2150, that was isolated off the Oregon coast using dilution-to-extinction culturing and that is affiliated with the Roseobacter clade. The 16S rRNA phylogeny showed that the strain was closely related to members of the RCA clade. The genome sequence suggests that strain HTCC2150 is an organoheterotroph carrying diverse metabolic potential, including a close relationship with phytoplankton.

Diversity Surveys and Evolutionary Relationships of aoxB Genes in Aerobic Arsenite-Oxidizing Bacteria

ABSTRACT A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis of AoxB protein sequences showed variable conservation levels and highlighted the conservation of essential amino acids and structural motifs. AoxB phylogeny of pure strains showed well-discriminated taxonomic groups and was similar to 16S rRNA phylogeny. Alphaproteobacteria-, Betaproteobacteria-, and Gammaproteobacteria-related sequences were retrieved from environmental surveys, demonstrating their prevalence in mesophilic As-contaminated soils. Our study underlines the usefulness of the aoxB gene as a functional marker of aerobic As(III) oxidizers.

16S rRNA Phylogeny of Sponge-Associated Cyanobacteria

ABSTRACT Phylogenetic analyses of 16S rRNA sequences of sponge-associated cyanobacteria showed them to be polyphyletic, implying that they derived from multiple independent symbiotic events. Most of the symbiont sequences were affiliated to a group of Synechococcus and Prochlorococcus species. However, other symbionts were related to different groups, such as the Oscillatoriales.

A mitochondrial 12S and 16S rRNA phylogeny of critical genera of Phoridae (Diptera) and related families of Aschiza

Phylogenetic analysis of mitochondrial 12S and 16S rRNA gene sequences supports the monophyly of the Phoridae. Within this family the Phorinae clade includes two aberrant termitophilous subfamilies, the Thaumatoxeninae and the Termitoxeniinae, which cluster with Dohrniphora and Diplonevra. These two genera include termitophiles and parasitoids of termites, so we hypothesize that these termitophilous phorids are a monophyletic group. While the data neither refute nor support the assumed monophyly of the Metopininae, the genera of this subfamily were not monophyletic in our analysis, but fell into two subclades that correspond with the tribes Metopinini and Gymnophorini.