Correlation of cutaneous blood microcirculation parameters with biological age in diabetic patients

Известно, что с возрастом и при ряде хронических заболеваний происходит снижение реактивности микроциркуляторного звена кровообращения. Целью данного исследования стала оценка взаимосвязи параметров кожной микроциркуляции с биологическим и хронологическим возрастом у пациентов с сахарным диабетом. В исследование были включены 11 человек с сахарным диабетом [медиана возраста 57 (51; 64) лет]; биологический возраст определяли с помощью калькулятора Aging.AI. Показатели кожной микроциркуляции оценивали с помощью метода лазерной допплеровской флоуметрии в ходе окклюзионно-теплового теста. Для анализа взаимосвязи количественных параметров рассчитывали коэффициенты ранговой корреляции Спирмена. Были выявлены значимые множественные отрицательные корреляции биологического возраста с показателями реактивности микрососудов как при тепловом, так и при окклюзионном воздействии (сила корреляций от -0,618 до -0,97, p<0,05). У лиц с сахарным диабетом снижение реактивности микрососудов в большей мере ассоциировано с биологическим возрастом, чем с хронологическим. It has been established that the age together with the number of chronic diseases cause the decrease of the reactivity of the microcirculatory bed. This study aims to evaluate the relationship between cutaneous microcirculation parameters and biological and chronological age of patients with diabetes mellitus. 11 diabetic patients (median age 57 (51; 64) years) were examined in course of this study; biological age was figured by Aging.AI calculator. Cutaneous microcirculation parameters were measured by laser Doppler flowmetry with an occlusion-heating test. Spearman’s rank correlation coefficients were calculated to analyze the relationships between quantitative parameters. Significant multiple negative correlations of biological age and microvascular reactivity indices on exposure to both heat and occlusion (correlation strength from -0,618, to -0,97, p<0,05) were found. Diabetic patients have decreased microvascular reactivity that is more associated with biological age than with chronological age.

Effects of Various Exercises on the Microvascular Reactivity of the Skin of the Knee Joint in Middle-Aged and Elderly Women

The objective of this study was to investigate the effects of square dance and brisk walking with the same frequency on the microvascular reactivity of the knee joint of elderly and middle-aged women. For the first time in Enshi city, women aged 55 to 65 years old were selected for experimental subjects, which included 15 in square dance group, 15 in Taijiquan group, 15 in fast walking group and 15 in control group. The changes in the skin microvascular reactivity of the knee joint were studied. The effects of various exercise regimens on the knee joint were evaluated. The results after the experiment, compared with the pre-experiment group, the increase rate of MBP in Taiji group increased significantly, but there was no significant change in square dance group, brisk walk group and control group (P > 0.05). The MBP in control group decreased in different degrees but not significantly (P > 0.05). The change in the AVBC rate in the Taijiquan group was significant, but not significantly so compared to the control group. The same results were obtained in the other groups. Hence, the increase rate of MBP in Taijiquan group was significantly higher than that in square dance group, fast walking group and control group, the increase rate of MBP in square dance group was significantly higher than that in control group, and the increase rate of AVBC in Taijiquan group was significantly higher than that in fast walking group and control group.

Vitamin C Improves Microvascular Reactivity and Peripheral Tissue Perfusion in Septic Shock Patients

Background Vitamin C has potential protective effects through anti-oxydant and anti-inflammatory properties. However, the effect of Vitamin C supplementation on microvascular function and peripheral tissue perfusion in human sepsis remains unknown. We aimed to determine vitamin C effect on microvascular endothelial dysfunction and peripheral tissue perfusion in septic shock patients. Methods Patients with septic shock were prospectively included after initial resuscitation. Bedside peripheral tissue perfusion and skin microvascular reactivity in response to acetylcholine iontophoresis in the forearm area were measured before and 1 hour after intravenous Vitamin C supplementation (40 mg/kg). Norepinephrine dose was not modified during the studied period. Results We included 30 patients with septic shock. SOFA score was 11 [8–14], SAPS II was 66 [54-79] and in-hospital mortality was 33%. Half of these patients had vitamin C deficiency at inclusion. Vitamin C supplementation strongly improved microvascular reactivity (AUC 2263 [430-4246] vs 5362 [1744-10585] UI, p=0.0004). In addition, Vitamin C supplementation improved mottling score (p=0.06), finger-tip (p=0.0003) and knee capillary refill time (3.7 [2.6-5.5] vs 2.9 [1.9-4.7] s, p<0.0001), as well as and central-to-periphery temperature gradient (6.1 [4.9-7.4] vs 4.6 [3.4-7.0] °C, p<0.0001). The beneficial effects of Vitamin C were observed both in patients with or without Vitamin C deficiency. Conclusion In resuscitated septic shock patients, vitamin C supplementation improved peripheral tissue perfusion and microvascular reactivity whatever plasma levels of vitamin C.

Effects of n-3 Polyunsaturated Fatty Acid-Enriched Hen Egg Consumption on the Inflammatory Biomarkers and Microvascular Function in Patients with Acute and Chronic Coronary Syndrome—A Randomized Study

This study aimed to test the effect of n-3 polyunsaturated fatty acid (PUFA)-enriched hen egg consumption on serum lipid and free fatty acid profiles, inflammatory and oxidative stress biomarkers, and microvascular reactivity in patients with coronary artery disease (CAD). Forty CAD patients participated in this study. Of those, 20 patients had acute CAD (Ac-CAD), and 20 patients had chronic CAD (Ch-CAD). The control group (N = 20) consumed three regular hen eggs/daily (249 mg n-3 PUFAs/day), and the n-3 PUFAs group (N = 20) consumed three n-3 PUFA-enriched hen eggs/daily (1053 g n-3 PUFAs/day) for 3 weeks. Serum n-3 PUFA concentration significantly increased (in all CAD patients), while LDL cholesterol and IL-6 (in Ac-CAD patients), and hsCRP and IL-1a (in all CAD patients) significantly decreased in the n-3 PUFAs group. Glutathione peroxidase (GPx) activity significantly decreased, and forearm skin microvascular reactivity in response to vascular occlusion (postocclusive reactive hyperemia (PORH)) remained unchanged in both the n-3 PUFAs and control groups in total CAD, Ac-CAD, and Ch-CAD patients. Potentially, n-3 PUFA-enriched hen eggs can change the free fatty acid profile to a more favorable lower n6/n3 ratio, and to exhibit mild anti-inflammatory effects but not to affect microvascular reactivity in CAD patients.