AbstractIn flow cytometers, ideally each detector receives photons from one specific fluorochrome. However, photons usually end up in different detectors too (fluorescence spillover). ‘Compensation’ is a process that removes this extra signal from all detectors other than the primary detector dedicated to that fluorochrome. Post ‘compensation’, the measurement error of spillover signals become evident as spreading of the data. Spillover reduces the ability to resolve single positive from double positive cell populations. For successful multi-color panel design, it is important to know the expected spillover. The Spillover Spread Matrix (SSM) can be used to estimate the spillover spread, but the outcome is heavily influenced by detector sensitivity. In short, the same single stained control sample produces different spillover spread values when detector sensitivity are altered. Many researchers unknowingly use this artifact to “reduce” the spread by decreasing detector sensitivity. This can result in reduced sensitivity and diminished capacity to resolve cell populations. In this article, we introduce ‘Range’ as an alternative tool that can predict the spillover independent of detector sensitivity. ‘Range’ is also independent of dynamic range, that allows to compare spread values between different types of instruments, something not possible using SSM.