Molecular identification of isolates from local microorganisms as potential biofertilizer

<p>Local microorganisms (MOL) are liquid fertilizers commonly used by farmers to help increase crop production. Beneficial microbes in MOL need to characterize their interactions and ability to produce growth drive compounds. The purpose of this research is to identify the superior microbial isolates from MOL made by farmers from Cibodas Lembang Bandung, Indonesia that can produce phytohormones as biofertilizers. The results of the microbial selection of MOL derived from three best microbes are 1A-2 NFB, 4A-1 NFB, and 4B-1 NFB with the ability to produce auxin, i.e., 19.41 ppm, 17.18 ppm, and 10.59 ppm, respectively. The compatibility test between the three isolates showed negative results so that it was possible to apply three microbes as a consortium. The results of a molecular identification with a 16S rRNA analysis indicate strain microbe 1A-2 NFB: <em>Bacillus cereus</em> (99.88% homology), 4A-1 NFB: <em>Bacillus cereus</em> (99.76% homology), and 4B-1 NFB: <em>Lysinibacillus</em> sp. (99.88% homology).</p>

Reshuffle minimisation to improve storage yard operations efficiency

There are many ways to measure the efficiency of the storage area management in container terminals. These include minimising the need for container reshuffle especially at the yard level. In this paper, we consider the container reshuffle problem for stacking and retrieving containers. The problem was represented as a binary integer programming model and solved exactly. However, the exact method was not able to return results for large instances. We therefore considered a heuristic approach. A number of heuristics were implemented and compared on static and dynamic reshuffle problems including four new heuristics introduced here. Since heuristics are known to be instance dependent, we proposed a compatibility test to evaluate how well they work when combined to solve a reshuffle problem. Computational results of our methods on realistic instances are reported to be competitive and satisfactory.

The Suitability of Compatibility Test Results with Gel Method between Diagnostic Grifols Gel Coombs and Diamed-Identification

A compatibility test as part of a pre-transfusion test is mandatory to ensure blood compatibility between patients anddonors. Diamed-ID as the first gel-based product is commonly used as a reference for the compatibility test. The presence ofnew products such as DG Gel Coombs encourages research to compare them with reference methods. This study aimed toanalyze the suitability of DG Gel Coombs to Diamed-ID in the compatibility test with the same sample. This cross-sectionalanalytic observational study was conducted during November 2017-February 2018 at the Blood Transfusion Unit Dr.Soetomo Hospital, Surabaya using blood samples (n=40), which met the inclusion and exclusion criteria. Simultaneoustesting of the two products was according to the manufacturing requirements of each product (using different LISS reagentsfor red blood cell suspensions and centrifugation arrangements). The suitability of results was tested with Cohen's kappaand significant differences with McNemar. There was a minimum suitability of DG Gel Coombs to Diamed-ID for majorcompatibility tests, κ 0.307 (95% CI: -0.029-0.643), significance 0.007 (p < 0.05) and moderate for minor, κ 0,678 (95% CI:0.454-0.903), significance <0.0001 (p < 0.05). McNemar's significance was 0.016 (p < 0.05) for major compatibility test and0.031 (p < 0.05) for minor. Referring to Diamed-ID's results, false negatives were found on DG Gel Coombs for majorcompatibility tests (n=7) and minor (n=6). The suitability of results from DG Gel Coombs and Diamed-ID is not strong forcompatibility testing.