Design and manufacture of three-dimensional auxetic warp-knitted spacer fabrics based on re-entrant and rotating geometries

Warp knitting technology is a fabric-forming technologies that is very suitable to fabricate three-dimensional (3D) auxetic fabrics due to its high efficiency and powerful pattern designing possibilities. In this study, two typical auxetic geometries, namely the re-entrant hexagonal network and rotating square solids, were selected as the design prototypes for the design and manufacture of 3D warp-knitted spacer fabrics. While two 3D warp-knitted spacer fabric structures with representative units of different sizes designed based on the re-entrant hexagonal geometry were manufactured by using a RD7 double needle bar Raschel machine with seven yarn guide bars, two 3D jacquard warp-knitted spacer fabrics with different base fabric structures designed based on the rotating squares geometry were fabricated by using a RDPJ4/2 double needle bar jacquard machine with two ground yarn guide bars and four jacquard guide bars. The Poisson’s ratios of these 3D warp-knitted fabrics in the course direction and wale direction were evaluated respectively through constant-rate tensile tests. The results revealed that the re-entrant hexagonal fabric structure with double chain stitches has auxetic behavior across a wide range of tensile strains along the course direction, while the rotating square fabric structure with elastic chain stitches as the base is auxetic within a narrow range of tensile strains along the wale direction. The study provides an alternative method to directly produce auxetic warp-knitted spacer fabrics through a single knitting process instead of using an additional post-compression and heat-setting process.

Cortical Thickness Adaptive Response to Mechanical Loading Depends on Periosteal Position and Varies Linearly With Loading Magnitude

The aim of the current study was to quantify the local effect of mechanical loading on cortical bone formation response at the periosteal surface using previously obtained μCT data from a mouse tibia mechanical loading study. A novel image analysis algorithm was developed to quantify local cortical thickness changes (ΔCt.Th) along the periosteal surface due to different peak loads (0N ≤ F ≤ 12N) applied to right-neurectomised mature female C57BL/6 mice. Furthermore, beam analysis was performed to analyse the local strain distribution including regions of tensile, compressive, and low strain magnitudes. Student’s paired t-test showed that ΔCt.Th in the proximal (25%), proximal/middle (37%), and middle (50%) cross-sections (along the z-axis of tibia) is strongly associated with the peak applied loads. These changes are significant in a majority of periosteal positions, in particular those experiencing high compressive or tensile strains. No association between F and ΔCt.Th was found in regions around the neutral axis. For the most distal cross-section (75%), the association of loading magnitude and ΔCt.Th was not as pronounced as the more proximal cross-sections. Also, bone formation responses along the periosteum did not occur in regions of highest compressive and tensile strains predicted by beam theory. This could be due to complex experimental loading conditions which were not explicitly accounted for in the mechanical analysis. Our results show that the bone formation response depends on the load magnitude and the periosteal position. Bone resorption due to the neurectomy of the loaded tibia occurs throughout the entire cross-sectional region for all investigated cortical sections 25, 37, 50, and 75%. For peak applied loads higher than 4 N, compressive and tensile regions show bone formation; however, regions around the neutral axis show constant resorption. The 50% cross-section showed the most regular ΔCt.Th response with increased loading when compared to 25 and 37% cross-sections. Relative thickness gains of approximately 70, 60, and 55% were observed for F = 12 N in the 25, 37, and 50% cross-sections. ΔCt.Th at selected points of the periosteum follow a linear response with increased peak load; no lazy zone was observed at these positions.

Can barb thread design improve the pullout strength of bone screws?

Aims To draw a comparison of the pullout strengths of buttress thread, barb thread, and reverse buttress thread bone screws. Methods Buttress thread, barb thread, and reverse buttress thread bone screws were inserted into synthetic cancellous bone blocks. Five screw-block constructs per group were tested to failure in an axial pullout test. The pullout strengths were calculated and compared. A finite element analysis (FEA) was performed to explore the underlying failure mechanisms. FEA models of the three different screw-bone constructs were developed. A pullout force of 250 N was applied to the screw head with a fixed bone model. The compressive and tensile strain contours of the midsagittal plane of the three bone models were plotted and compared. Results The barb thread demonstrated the lowest pullout strength (mean 176.16 N (SD 3.10)) among the three thread types. It formed a considerably larger region with high tensile strains and a slightly smaller region with high compressive strains within the surrounding bone structure. The reverse buttress thread demonstrated the highest pullout strength (mean 254.69 N (SD 4.15)) among the three types of thread. It formed a considerably larger region with high compressive strains and a slightly smaller region with high tensile strains within the surrounding bone structure. Conclusion Bone screws with a reverse buttress thread design will significantly increase the pullout strength. Cite this article: Bone Joint Res 2021;10(2):105–112.

Auxetic behavior of warp knitted fabric under repeating tension

In our previous study, a novel class of auxetic warp knitted fabrics were developed and their auxetic behaviors were studied under a single tensile test. However, during daily use, the fabrics are usually subjected to repeating tension rather than single tension. Therefore, the durability of the fabrics’ auxetic performance is of great importance. So far, the auxetic behavior of fabrics under repeating tension has not systematically been investigated. In this paper, we report a study on the auxetic behavior of warp knitted fabrics under repeating tension. All the fabric samples were subjected to a repeating tensile test within a tensile strain of 25% until 100 tensile cycles. The results show that the fabrics can keep their auxetic effect in both course and wale testing directions after 100 tensile cycles, and the auxetic effect in the wale direction is retained longer under higher tensile strains than that under lower tensile strains with the increase of tensile cycles. The results also indicate that auxetic stability in the course direction is much better than that in the wale direction. We hope that this study can offer useful information to improve the auxetic stability of auxetic fabrics for practical use.

Interleukin-1β Induced Matrix Metalloproteinase Expression in Human Periodontal Ligament-Derived Mesenchymal Stromal Cells under In Vitro Simulated Static Orthodontic Forces

The periodontal ligament (PDL) responds to applied orthodontic forces by extracellular matrix (ECM) remodeling, in which human periodontal ligament-derived mesenchymal stromal cells (hPDL-MSCs) are largely involved by producing matrix metalloproteinases (MMPs) and their local inhibitors (TIMPs). Apart from orthodontic forces, the synthesis of MMPs and TIMPs is influenced by the aseptic inflammation occurring during orthodontic treatment. Interleukin (IL)-1β is one of the most abundant inflammatory mediators in this process and crucially affects the expression of MMPs and TIMPs in the presence of cyclic low-magnitude orthodontic tensile forces. In this study we aimed to investigate, for the first time, how IL-1β induced expression of MMPs, TIMPs and how IL-1β in hPDL-MSCs was changed after applying in vitro low-magnitude orthodontic tensile strains in a static application mode. Hence, primary hPDL-MSCs were stimulated with IL-1β in combination with static tensile strains (STS) with 6% elongation. After 6- and 24 h, MMP-1, MMP-2, TIMP-1 and IL-1β expression levels were measured. STS alone had no influence on the basal expression of investigated target genes, whereas IL-1β caused increased expression of these genes. In combination, they increased the gene and protein expression of MMP-1 and the gene expression of MMP-2 after 24 h. After 6 h, STS reduced IL-1β-induced MMP-1 synthesis and MMP-2 gene expression. IL-1β-induced TIMP-1 gene expression was decreased by STS after 6- and 24-h. At both time points, the IL-1β-induced gene expression of IL-1β was increased. Additionally, this study showed that fetal bovine serum (FBS) caused an overall suppression of IL-1β-induced expression of MMP-1, MMP-2 and TIMP-1. Further, it caused lower or opposite effects of STS on IL-1β-induced expression. These observations suggest that low-magnitude orthodontic tensile strains may favor a more inflammatory and destructive response of hPDL-MSCs when using a static application form and that this response is highly influenced by the presence of FBS in vitro.