DNA methylation architecture of the ACE2 gene in nasal cells of children

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has led to the global coronavirus disease 2019 (COVID-19) pandemic. SARS-CoV-2 enters cells via angiotensin-Converting Enzyme 2 (ACE2) receptors, highly expressed in nasal epithelium with parallel high infectivity.1,2 The nasal epigenome is in direct contact with the environment and could explain COVID-19 disparities by reflecting social and environmental influences on ACE2 regulation. We collected nasal swabs from anterior nares of 547 children, measured DNA methylation (DNAm), and tested differences at 15 ACE2 CpGs by sex, age, race/ethnicity and epigenetic age. ACE2 CpGs were differentially methylated by sex with 12 sites having lower DNAm (mean = 12.71%) and 3 sites greater DNAm (mean = 1.45%) among females relative to males. We observed differential DNAm at 5 CpGs for Hispanic females (mean absolute difference = 3.22%) and lower DNAm at 8 CpGs for Black males (mean absolute difference = 1.33%), relative to white participants. Longer DNAm telomere length was associated with greater ACE2 DNAm at 11 and 13 CpGs among males (mean absolute difference = 7.86%) and females (mean absolute difference = 8.21%), respectively. Nasal ACE2 DNAm differences could contribute to our understanding COVID-19 severity and disparities reflecting upstream environmental and social influences. Findings need to be confirmed among adults and patients with risk factors for COVID-19 severity.

Work Patterns and Financing College: A Descriptive Regional Report regarding Students at Hispanic-Serving Institutions in New Mexico and Texas

College students at 14 Hispanic-Serving Institutions (HSIs) in New Mexico and Texas were surveyed about their experiences in and perceptions of higher education. Three primary foci were students’ employment status, work commitments, and means of financing college. Most of the informants reported working while in college and, similar to previously reported national averages, 69.4% of the informants were actively employed. Twice as many of the actively employed informants worked off campus as on campus and over three-quarters of employed students reported working part-time. There were no significance differences in these areas by gender, ethnicity, or even when broken out as Latinas, Latinos, non-Hispanic females and non-Hispanic males but students of non-traditional age reported a work commitment at significantly higher levels. For hours of work per week, there were also no significant differences by gender, ethnicity, and for the four possible subsets (Latinas, Latinos, etc.) but being a non-traditional aged student and being married/cohabiting were associated with working more hours at statistically significant levels. Students at the HSIs in New Mexico also reported more hours of work at statistically significant levels. Differences by gender, ethnicity, age, relational status, and state were found for means of funding college.

Racial Differences of Attitudes Towards Medical Marijuana Use Among American Older Adults

Background: Extensive evidence documented that medical marijuana (MM) could be used for pain management with fewer side effects. Although MM legalization remains controversial as it is perceived to be a “gateway drug”, more states are striving for MM legalization and calling for health insurance coverage. Racial difference in the use of opioids for pain management is well-documented, which may also exist in the use of MM. This study explored an understudied topic on how people from different racial backgrounds perceive MM and its behind mechanism. Method: Data is from Health and Retirement Study Wave 2018 who answered the special modules on MM (n=1340). The attitude is proxied by two dichotomous measures on whether they think MM is acceptable and would lead to hard drug. Logistic regressions were used to evaluate the relationships between attitude and race, adjusted for sociodemographic, health and mental health, and MM knowledge. Moderating effects of diseases and socioeconomic-status (SES) were tested using interaction terms. Results: Older adults who hold positive attitudes towards MM are more likely to be younger, high SES, using opioids. Hispanics and Blacks are more negative towards MM relative to White counterparts. Blacks with cancer (OR=.30) are less likely to believe MM is acceptable, whereas blacks with arthritis are more likely to accept MM (OR=2.08) compared to Whites. Hispanic females (OR=.26) are more likely to oppose MM while Hispanics with higher education background (OR=4.3) are more likely to hold positive attitudes. Implication: The results may guide development of future guidelines on MM prescription.

Racial and Ethnic Differences in Incidence and Disease Specific Survival of Multiple Myeloma- New Mexico Tumor Registry Based Study

Background: Multiple Myeloma is a plasma cell neoplasm which is characterized by an increase in monoclonal proteins or light chains and end-organ damage. Multiple Myeloma accounts for 1.8% of all new cancer cases in the United States, with an estimated 32,110 new cases annually. In population-based studies, African Americans have a higher incidence of Myeloma and inferior outcomes when compared to Caucasians. Disparities in the incidence of Myeloma for other races in the United States also exist but are not well studied.(1). Analyses of Surveillance, Epidemiology, and End Results program (SEER) data showed that Hispanics had poor or delayed access to novel agents and transplant, and poor overall survival.(2). Data for Native Americans was not reported due to small or insignificant numbers. New Mexico is a majority minority state, with approximately 1 million Hispanics residing in the state, constituting 49.1% of the total population and the largest statewide percentage of Hispanic residents nationally. (3) New Mexico also has a sizable population of 219,237 Native Americans, who make-up nearly 10.5% of the state's entire population. (3) Hence, the significant minority population in New Mexico allows the comparison of incidence between racial and ethnic subgroups as well as disease specific survival of Myeloma in New Mexico residents using the New Mexico Tumor Registry. Methods: We utilized data from the New Mexico Tumor Registry to study patients with a documented diagnosis of Myeloma. Descriptive statistics including the incidence rates of myeloma and Kaplan-Meier product-limit methods to assess cause-specific survival for incident myeloma cases diagnosed among New Mexico residents during the time period 2008-2017. Other variables including transplantation rates were also studied but are not reported in this abstract. Results: Men had higher incidence of Myeloma compared to females (p<0.01) and Hispanic females had a higher incidence compared to Non-Hispanic white females (p<0.05). The incidence of Myeloma (for both sexes) was slightly increased in Hispanics (5.6 /per 100,000, CI- 5.1,6.2) and Native Americans (6.3/per 100,000, CI- 5.1,7.7) compared to Non- Hispanic Caucasians (5.1/per 100,000, CI- 4.7,5.5). (Fig. 1) However, these differences were not statistically significant. The incidence of multiple myeloma has been stable for all racial-ethnic group examined during 1981-2017. (Fig. 2) Modest differences in survival among Non-Hispanic whites, Hispanics, and American Indians were not statistically significant (log-rank test, p=0.3907). (Fig.3) Conclusion: Racial-ethnic differences in incidence and disease specific survival of Multiple Myeloma were evident but were not statistically significant in New Mexico, except incidence of Multiple Myeloma among Hispanic females compared to Non-Hispanic white females. Further study of disease-related factors (high risk disease, mutational profiles, stage at diagnosis) and patient-related factors (access to standard treatments, transplantation or clinical trials) may be needed to understand these differences and better care for the patients. Disclosures Andritsos: Innate Pharma: Consultancy, Honoraria.

Racial/Ethnic Differences in Health Behaviors and Its Roles on Depressive Symptoms among Young Female Adults

This study explores the role of health behaviors on depressive symptoms across young adult females and differences in the relationship across race/ethnicity. The data come from the National Longitudinal Survey of Youth 1979 Child and Young Adult. Seven hundred and seven non-Hispanic White females, 592 African American females, and 349 Hispanic females were selected. Multiple linear regression and logistic regression analyses were conducted. African American and Hispanic females were more likely to eat fast food than non-Hispanic Whites. African Americans reported that they ate fruit less frequently in comparison with non-Hispanic Whites. Fruit intake was related to lower levels of depressive symptoms. Hispanics moderated the association between fruit intake and depressive symptoms. Females should be encouraged to eat more fruit during young adulthood in order to diminish the likelihood of depressive symptoms. In addition, strategies for promoting healthy behaviors should consider the varied effects of race/ethnicity on depressive symptoms among young female adults.

The Association Between Functional Assessment and Structural Brain Biomarkers in an Ethnically Diverse Sample With Normal Cognition, Mild Cognitive Impairment, or Dementia

Objective To investigate the association between the functional activities questionnaire (FAQ) and brain biomarkers (bilateral hippocampal volume [HV], bilateral entorhinal volume [ERV], and entorhinal cortical thickness [ERT]) in cognitively normal (CN) individuals, mild cognitive impairment (MCI), or dementia. Method In total, 226 participants (137 females; mean age = 71.76, SD = 7.93; Hispanic Americans = 137; European Americans = 89) were assessed with a comprehensive clinical examination, a neuropsychological battery, a structural magnetic resonance imaging, and were classified as CN or diagnosed with MCI or dementia. Linear regression analyses examined the association between functional activities as measured by the FAQ on brain biomarkers, including HV, ERV, and ERT, controlling for age, education, global cognition, gender, and ethnicity. Results The FAQ significantly predicted HV, ERV, and ERT for the entire sample. However, this association was not significant for ERV and ERT when excluding the dementia group. The FAQ score remained a significant predictor of HV for the non-dementia group. Age, education, gender, ethnicity, Montreal Cognitive Assessment score, and FAQ were also significant predictors of HV for the overall sample, suggesting that younger Hispanic females with fewer years of education, higher global mental status, and better functioning, were more likely to have larger HV. Conclusion FAQ scores were related to HV in older adults across clinical groups (CN, MCI, and dementia), but its association with the entorhinal cortex was driven by individuals with dementia. Demographic variables, including ethnicity, additionally influenced these associations.

DNA Methylation Architecture of the ACE2 gene in Nasal Cells

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has led to the global coronavirus disease 2019 (COVID-19) pandemic. SARS-CoV-2 enters cells via angiotensin-Converting Enzyme 2 (ACE2) receptors, highly expressed in nasal epithelium with parallel high infectivity.1,2 The nasal epigenome is in direct contact with the environment and could explain COVID-19 disparities by reflecting social and environmental influences on ACE2 regulation. We collected nasal swabs from anterior nares of 547 children, measured DNA methylation (DNAm), and tested differences at 15 ACE2 CpGs by sex, age, race/ethnicity and epigenetic age. ACE2 CpGs were differentially methylated by sex with 12 sites having lower DNAm (mean=12.71%) and 3 sites greater DNAm (mean=1.45%) among females relative to males. We observed differential DNAm at 5 CpGs for Hispanic females (mean absolute difference=3.22%) and lower DNAm at 8 CpGs for Black males (mean absolute difference=1.33%), relative to white participants. Longer DNAm telomere length was associated with greater ACE2 DNAm at 11 and 13 CpGs among males (mean absolute difference=7.86%) and females (mean absolute difference=8.21%), respectively. Nasal ACE2 DNAm differences could contribute to our understanding COVID-19 severity and disparities reflecting upstream environmental and social influences.

1102 Relationship Between Emotional Distress And Sleep Duration Among Hispanics Using The 2018 National Health Interview Survey Dataset

Introduction Although Hispanics experience a high level of shorter sleep duration (< 7 hrs./24 period), a clear mechanism or cause is lacking. Previous research indicate that emotional distress may explain the burden of shorter sleep among blacks. Applying these findings to Hispanics, we investigated whether emotional distress explains the burden of short sleep duration (< 7 hrs.) among Hispanics and if this relationship varies by sex Methods We used data from the 2018 National Health Interview Survey (NHIS) dataset, a nationally representative sample, in which only Hispanic ethnicity participants (N=3,091) were analyzed. Average sleep duration was self-reported and measured in hours. Emotional distress was measured using Kessler 6, which measures how an individual felt over the past 30 days: nervous, hopeless, restless/fidgety, depressed, effortful and worthless. To assess the association between short sleep duration and emotional distress, we performed Pearson correlation, hierarchical regression analyses, and stratified this relationship by sex to determine if this relationship differed between males and females, adjusting for covariates. Results Of the total sample of 3,091 Hispanics, 1,762 were female, and 1,329 were male. Sleep duration and emotional distress were negatively correlated among females (r = -.27, p <.001) and males (r=-.18, p <.001). Among Hispanic females, sleep duration significantly predicted emotional distress, β = -.27, t = -11.60, p <.001, and explained a significant portion of variance in emotional distress, R2 = .07, F= 134.63, p <.001. While, among Hispanic males, sleep significantly predicted emotional distress (β = -.18, t =-6.5, p <.001) and explained a significant portion of the variance in emotional distress (R2 = .03, F= 42.37, p <.001). Conclusion Our findings indicate that a negative sleep-ED relationship, suggesting that shorter sleep was predictive of higher levels of emotional distress among Hispanics and that this relationship is greater among Hispanic females, compared males. Support K01HL135452, R01MD007716, R01HL142066, and K07AG052685