scholarly journals Superovulatory responses using pregnant mare serum gonadotropin hormone in Murrah buffalo cows

2021 ◽  
Vol 888 (1) ◽  
pp. 012016
Author(s):  
L Praharani ◽  
R S G Sianturi ◽  
D A Kusumaningrum ◽  
S A Asmarasari
Keyword(s):  
Artificial Insemination ◽  
Fixed Time ◽  
Transfer Program ◽  
Murrah Buffalo ◽  
Pregnant Mare Serum Gonadotropin ◽  
Embryo Transfer Program ◽  
Treatment Administration ◽  
Serum Gonadotropin ◽  
The Mean ◽  
Preliminary Study

Abstract Superovulation plays an important role in embryo transfer program. A preliminary study carried out in the Research Institute for Animal Production evaluated superovulatory responses in Murrah buffalo cows using pregnant mare serum gonadotropin (PMSG) hormone. The three buffalo cows were estrus synchronized using 5 ml prostaglandin (PGF) twice, with an interval of 11 days. PMSG was injected intra-muscularly 3000 IU on day-10 after estrus. Prostaglandins were administered 48 hours after PMSG injection. Fixed-Time artificial insemination (FTAI) was carried out at 72 hours after the last PGF treatment. Administration of hCG 2 ml/head was given at the time of FTAI. A non-surgery flushing was performed on day 6 after FTAI. Parameters observed using ultrasonography (USG) were diameter of follicle (DFL), total follicle (TFL), and number embryos (NE). Data were analyzed descriptively. The mean of DFL before PMSG treatment was 8.2 mm and after PMSG treatment was 12.5 mm. The mean of TFL before PMSG treatment was 7.7 and after PMSG treatment was 16.1. The NE obtained was one degenerative embryo. Superovulation using PMSG increased TCL and DFL. It can be concluded that the Murrah buffalo cows superovulated by PMSG showed a good response but no transferable embryo was found.

scholarly journals Comparative Study Between Different Methods of Insemination in Iraqi Does

2011 ◽  
Vol 35 (2) ◽  
pp. 95-104
Author(s):  
HA . AL-Mutar
Keyword(s):  
Comparative Study ◽  
Pregnancy Rate ◽  
Breeding Season ◽  
Artificial Insemination ◽  
Fixed Time ◽  
Experimental Animals ◽  
Pregnant Mare Serum Gonadotropin ◽  
Abdominal Palpation ◽  
Serum Gonadotropin ◽  
In Pregnancy

Present experimental was carried out on 22 does 2-4 years old. The aim was to induction of estrous in non-breeding season using impregnated sponge with 20 mg of medroxy progesterone acetate (MPA) for 13 days and with an i/m injection of 500 IU Pregnant Mare Serum Gonadotropin (PMSG) hormone 24 hrs before sponge withdrawal and to compare the pregnancy rate using three different methods of insemination; natural , cervical and laparoscopic artificial insemination at fixed time 24-48 hrs after estrous onset. All does were showed signs of estrous (100%), the estrous time was 46.9±4.90hrs (24-60hrs) after sponge withdrawal. While estrous length was 37.09±1.91 (24–72hrs). All experimental animals followed up by abdominal palpation, ultrasonography at 30, 60 and 90 days post-insemination, while the laparoscopic examination was performed at day 30 post-insemination, to improve the efficiently of the different methods of inseminations in pregnancy rate ultrasonographical diagnosed by rectal and abdominal methods of 14 does post-inseminations appeared that two were pregnant, seven suspected and five non pregnant. At day 60th , post-insemination the pregnancy diagnosis of (22) does appeared that (14) were pregnant, six suspected and two non pregnant. While at day 90, the examination of all does appeared that (16) animals were pregnant and six were not. The laparoscopic examination, at 30 days post-insemination showed that eight does were pregnant, two suspected and two were not pregnant. In conclusion that the pregnancy rate of laparoscopic insemination is (75%), comparisons to natural (66.7%) and cervical AI method (75%) but there is no significant different between them, while the kidding percentage was appear in natural insemination (75%), cervical (100%) and laparoscopic insemination (150%). The results of this study indicate that estrus can be efficiently induced in female goats during non-breeding season using 20mg MPA impregnated spongewith 500IU PMSG.

scholarly journals Gestational losses after innovating with two embryos in a fixed-time embryo transfer program

2021 ◽  
Vol 14 (10) ◽  
Author(s):  
Carla Neves Barbosa ◽  
Felipe De Col ◽  
Juan Carlos Domínguez Fernández de Tejerina ◽  
Rodolfo Cassimiro de Araujo Berber
Keyword(s):  
Embryo Transfer ◽  
Birth Rate ◽  
Considerable Increase ◽  
Hormonal Treatment ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Corpora Lutea ◽  
Transfer Program ◽  
Embryo Transfer Program ◽  
Group 1

The aim of this study was to quantify the pregnancy rate after implantation of two embryos after FTET protocols, as well as to monitor pregnancy losses until parturition, evaluating, mainly, if this strategy results in more number of animals born. Therefore, 423 multiparous recipients were selected, standardized in terms of body score, who had high-quality corpora lutea. Animals were randomly divided into two groups according to one or two embryos transferred (1 embryo = Control, n = 237; 2 embryos = Group 1, n = 186). All recipients received the same hormonal treatment, which consisted of administering, on Day 0, 2 mL of estradiol benzoate (Gonadiol, ZOETIS) + 1.9 g multidose 1st use progesterone implant (CIDR, ZOETIS); on Day 8 the implants were removed + injected 0.4 mL of estradiol cypionate (E.C.P, ZOETIS) + 1.5 mL of eCG (Novormon, ZOETIS) + 1 mL of dinoprost tromethamine (Lutalyse, ZOETIS). The animals were evaluated by ultrasonography at 30 and 60 days after embryo transfer, to diagnose the success rate and embryo losses during this period. Furthermore, information was collected on births, length of gestation, number of twin births, number of childbirth assistance and the weight of the calves. The results showed that Group 1 had better success than the Control, with higher conception rates at 30 days (68.3% vs. 53.2%, P<0.001) and at 60 days (62.9% vs. 52.3%; P<0 .05). The number of animals born was also higher for Group 1 (53.3% vs. 43.3%, P<0.01). The percentage of twins born in Group 1 was 17.9%, and the animals had lower weight compared to the Control (34.29 + 7.36 vs 37.63 + 5.73, P<0.05). The length of pregnancy and the number of assistances were similar between groups. In conclusion, the strategy adopted in this experiment suggests a considerable increase in the calf birth rate, but losses during pregnancy and their mechanisms need to be elucidated.

10 EFFECTS OF INSEMINATION TIME, BREED, AND INSEMINATOR ON FERTILITY OF EWES INTRAUTERINALLY INSEMINATED WITH FROZEN - THAWED SEMEN IMPORTED FROM NEW ZEALAND

2007 ◽  
Vol 19 (1) ◽  
pp. 123 ◽  
Author(s):  
Y. Fukui ◽  
H. Kohno ◽  
T. Togari ◽  
T. Matsuoka ◽  
H. Imai
Keyword(s):  
New Zealand ◽  
Intrauterine Insemination ◽  
Fixed Time ◽  
Transfer Program ◽  
Chi Square ◽  
Frozen Semen ◽  
Embryo Transfer Program ◽  
Chi Square Test ◽  
Equine Chorionic Gonadotropin ◽  
Lambing Rate

Artificial insemination, especially with the use of frozen semen, is one of the important tools for embryo transfer program in sheep. The present study investigated the effects of insemination times, breeds, and two inseminators on the fertility of ewes intrauterinally inseminated with frozen–thawed ram semen imported from New Zealand. At 8 sheep farms located in Hokkaido, Japan, during the breeding season (October to December) in 2005, a total of 64 mature (1- to 6-year old) Suffolk (32 heads) and Polled Dorset (32 heads) ewes were used. The ewes were treated with controlled intravaginal drug release (CIDR containing 0.3 g progesterone; Pharmacia &amp; Upjohn, Ltd., Auckland, New Zealand) for 12 days and an injection of 500 IU equine chorionic gonadotropin one day before CIDR removal. The fixed-time intrauterine inseminations (early: 43–46 h; late: 47–50 h) after CIDR removal were performed using the frozen–thawed semen from a Suffolk and Polled Dorset ram by two inseminators. The effects of breeds (Suffolk and Polled Dorset), fixed-time insemination times (early and late phases), and two inseminators on pregnancy (number of pregnant ewes/number of ewes inseminated, 60 days after insemination) and lambing (number of lambed ewes/number of ewes inseminated) rates were analyzed by chi-square test. The prolificacy was compared by Student&apos;s t-test, and differences were also analyzed by Tukey&apos;s omega procedure. The effect of the different farms on fertility was not examined due to the small numbers of ewes per farm. Pregnancy (60.0 and 72.4%, respectively) and lambing (60.0 and 71.4%, respectively) rates were not significantly different between Suffolk and Polled Dorset ewes. The inseminators also did not affect pregnancy (62.6 and 68.8%) and lambing (62.6 and 67.7%) rates. For the insemination times, the lambing rate tended to be higher (P ≤ 0.10) in the early insemination than in the late insemination (76.7% and 53.6%, respectively). The present results show acceptable fertility in ewes inseminated with Suffolk and Polled Dorset frozen semen imported from New Zealand. The early intrauterine insemination (43–46 h after CIDR removal) tended to result in higher fertility than the late insemination (47–50 h after CIDR removal). From 38 lambed ewes, 60 newborn lambs were produced, and this has provided new blood lines of Suffolk and Polled Dorset sheep in Japan.

160 SUCCESSFUL PREGNANCY IN ESTRUS-SYNCHRONIZED HIMALAYAN TAHRS (HEMITRAGUS JEMLAHICUS) BY TRANSCERVICALLY INSEMINATING FRESH AND EXTENDED-CHILLED SEMEN COLLECTED BY ELECTROEJACULATION

2011 ◽  
Vol 23 (1) ◽  
pp. 182
Author(s):  
H. Yong ◽  
B.-S. Bae ◽  
S.-D. Kim ◽  
S.-H. Hyun
Keyword(s):  
Electrical Stimulation ◽  
Artificial Insemination ◽  
Doppler Ultrasonography ◽  
The Other ◽  
Successful Pregnancy ◽  
Pregnant Mare Serum Gonadotropin ◽  
Artificial Breeding ◽  
Hemitragus Jemlahicus ◽  
Serum Gonadotropin ◽  
Fresh Semen

In December 2009, 8 female Himalayan tahrs (Hemitragus jemlahicus) were synchronized by inserting CIDR devices for 13 or 15 days. One day before removal of the CIDRs, a combination of pregnant mare serum gonadotropin (PMSG) 400 IU and hCG 200 IU (PG600®, Intervet, Boxmeer, the Netherlands) was intramuscularly injected into each animal and PGF2α was injected at the time of CIDR withdrawal. Semen was collected by electrical stimulation, diluted, cooled to 5°C for 2 h, and maintained in a refrigerator before performing AI twice. Pregnancy diagnosis was conducted by Doppler ultrasonography on March 2, 2010. Four female Himalayan tahrs were impregnated, showing a fetus present in their uteri. In the first experiment in which CIDRs were inserted into the vagina of 4 Himalayan tahrs for 13 days, 3 of the tahrs were transcervically inseminated with fresh semen, whereas 1 was inseminated with frozen–thawed semen 33 h after CIDR removal. As a second insemination experiment, 46 h after CIDR removal, all 4 tahrs were inseminated with diluted (1:3) fresh semen. While the tahr that was later inseminated with frozen–thawed semen did not become pregnant, the other 3 Himalayan tahrs became pregnant. In the second experiment, CIDR devices were inserted into the vaginas of 4 Himalayan tahrs for 15 days. Extended (1:7)-chilled semen was used for transcervical insemination 42 and 49 h after CIDR withdrawal. When performing the second insemination, it was difficult or impossible to penetrate cervical canals. Thus, only 1 tahr became pregnant. These findings show 13 days of CIDR insertion, the dose of PMSG 400 IU and hCG 200 IU, and the use of PGF2α as beneficial for inducing oestrus synchronization, and prove that extended-chilled semen may also be used for artificial breeding of Himalayan tahrs. This is the first report of successful pregnancy induced by artificial insemination of fresh or extended-chilled semen in oestrus-synchronized Himalayan tahrs.

scholarly journals Superovulation response of Peranakan Ongole (PO) and Simmental cows after FSH stimulation in multiple ovulation and embryo transfer program

2021 ◽  
Vol 902 (1) ◽  
pp. 012044
Author(s):  
A F Lubis ◽  
A S Satyaningtijas ◽  
O P Lubis ◽  
W Kurniati ◽  
A Boediono
Keyword(s):  
Embryo Transfer ◽  
Artificial Insemination ◽  
Follicle Stimulating Hormone ◽  
Reproductive Technology ◽  
Transfer Program ◽  
Livestock Population ◽  
Reduced Dose ◽  
Multiple Ovulation ◽  
Short Period ◽  
Embryo Transfer Program

Abstract Multiple ovulation and embryo transfer (MOET) is a reproductive technology to increase the livestock population in a short period. The success of ovary stimulation programs is influenced by an individual’s response to follicle-stimulating hormone (FSH) stimulation. In this study embryo production was carried out on local cows represented by Peranakan Ongole (PO) cows and exotic cows represented by Simmental cows. FSH stimulation was performed on 10 PO cows and 10 Simmental cows. On Day-1 Cue Mate® (progesterone) was inserted intravaginally. FSH injection (400 mg) was carried out intramuscularly from D-10 at reduced dose with 12 hours intervals (Day-10: 100 mg; Day-11: 60 mg; Day-12: 40 mg per injection). On Day-12, prostaglandin (PGF2α) was injected and Cue Mate® was removed. Artificial insemination (AI) was performed 48 hours after PGF2α injection for 3 times at 12 hours intervals. The embryo was collected 6 days after the last AI (Day-21). Superovulation response was detected on 70% of PO cows and 90% of Simmental cows. The average number of transferable embryos in Simmental cows (9.11±7.27) was higher than PO cows (7.86±7.78). This research shows that Simmental cows are more responsive to FSH stimulation, and can produce more transferable embryos than PO cows.

scholarly journals Estrus Induction and Fertility Response following Ovsynch Alone and Ovsynch plus PRID Protocol in Postpartum Anestrus Surti buffaloes

2017 ◽  
Vol 12 (4) ◽  
Author(s):  
C. T. Khasatiya ◽  
H. R. Savani ◽  
Swati Saxena ◽  
M. D. Patel
Keyword(s):  
Artificial Insemination ◽  
Fixed Time ◽  
Conception Rate ◽  
Control Group ◽  
Control Groups ◽  
Treatment Groups ◽  
The Mean ◽  
And Control

The study was undertaken to find out the estrus induction and conception rate in 18 postpartum anestrus Surti buffaloes divided equally into three groups and treated with Ovsynch protocol alone and in combination with PRID, keeping one group as control. The use of Ovsynch alone and Ovsynch + PRID protocols with fixed-time artificial insemination resulted in 100% estrus induction with 66.66% and 50.00% conception rate at induced estrus, respectively, and the overall conception rate was 83.33% in both the groups. In untreated anestrus control (n=6), only four (66.66%) buffaloes exhibited spontaneous estrus within 120 days of follow-up and conceived giving the first service and overall conception rate as 75.00% and 66.66%, respectively. The mean number of services per conception did not differ significantly among the treatment and control groups. The service periods of buffaloes under both the treatment groups were significantly (p less than 0.05) shorter (57.00±2.81 and 61.20±3.11 days) than the control group (104.00±20.22 days). Thus, the Ovsynch and Ovsynch + PRID protocol can be successfully used to induce fertile estrus in postpartum anestrus buffaloes.

scholarly journals Nutzung einer terminorientierten Besamung beim Rind auf der Grundlage einer Prostaglandin – GnRH-Behandlung

2004 ◽  
Vol 47 (1) ◽  
pp. 27-36
Author(s):  
F. Becker ◽  
E. Kanitz ◽  
F. Schneider ◽  
G. Nürnberg ◽  
W. Kanitz
Keyword(s):  
Artificial Insemination ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Oestrous Cycle ◽  
Lh Surge ◽  
Dairy Heifers ◽  
65 H ◽  
The Mean ◽  
Timed Artificial Insemination ◽  
Predetermined Time

Abstract. Title of the paper: Practical application of timed artificial insemination in cattle using a GnRH and Prostaglandin treatment Three experiments were conducted to establish a regime of oestrous synchronization and artificial insemination at a predetermined time in dairy cattle. In experiment 1, dairy heifers (n = 43) were synchronized by administering PGF2α on day 5, 8, 11 or 14 and the LH surge was recorded. Release of the LH surge ranged from 57.0 ± 8.6 h to 76.3 ± 10.0 h depending upon the day of induced luteolysis. The release of LH was followed by ovulations. The day of PGF2α administration did not affect the interval from LH peak to ovulation. In experiment 2, heifers (n = 30) were synchronized on days 8, 11 or 14 of the oestrous cycle. Animals received an injection of GnRH 65 h after induction of luteolysis. Uniform intervals from PGF2α application to LH surge were observed in animals given PGF2α on day 11 and 14. Variation for this parameter decreased also in the group of animals where luteolysis was induced on day 8. There were no significant differences due to treatment in the mean interval from LH-peak to ovulation. In experiment 3, fixed time insemination was tested on the basis of data from Experiments 1 and 2. Synchronized heifers and high yielding cows received GnRH 65 hours after PGF2α administererd between d 8 and 14 of the oestrous cycle. Artificial insemination was carried out 13 h after GnRH application. Pregnancy rates show, that fixed time artificial insemination, independent of the recorded onset of estrus can result in high pregnancy rates. Pregnancy rates in animals at different locations were higher after induction of ovulation and timed artificial insemination (79.0, 76.0 and 47.1, 53.3 %) than in control groups with insemination after detected estrus (69.8, 63.2 and 38.6, 45.0 %).

Treatments for the synchronisation of bovine recipients for fixed-time embryo transfer and improvement of pregnancy rates

2012 ◽  
Vol 24 (1) ◽  
pp. 272 ◽  
Author(s):  
Gabriel A. Bó ◽  
Lucas Coelho Peres ◽  
Lucas E. Cutaia ◽  
Danilo Pincinato ◽  
Pietro S. Baruselli ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Transfer Program ◽  
Widespread Application ◽  
Wave Dynamics ◽  
Follicular Wave ◽  
Embryo Transfer Program ◽  
Conception Rates ◽  
On Farm

Although embryo transfer technology has been used commercially in cattle for many years, the inefficiency of oestrus detection, especially in recipients, has limited the widespread application of this technology. The most useful alternative to increase the number of recipients utilised in an embryo transfer program is the use of protocols that allow for embryo transfer without the need for oestrus detection, usually called fixed-time embryo transfer (FTET). Most current FTET protocols are based on progestin-releasing devices combined with oestradiol or GnRH, which control and synchronise follicular wave dynamics and ovulation. Conception rates to a single FTET have been reported to be similar to those after detection of oestrus, but pregnancy rates are higher because these treatments have increased the proportion of recipients that receive an embryo. Recent changes to treatments for FTET, such as the administration of eCG, have resulted in increased pregnancy rates and provide opportunities to make these treatments easier to perform on farm.

scholarly journals Bovine semen thermoresistance tests and their correlation with pregnancy rates after fixed-time artificial insemination

2019 ◽  
Vol 71 (6) ◽  
pp. 2085-2092
Author(s):  
R. Talini ◽  
L.E. Kozicki ◽  
F.R. Gaievski ◽  
G. Polo ◽  
L.G.F. Lima ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Sperm Motility ◽  
Negative Correlation ◽  
Artificial Insemination ◽  
Semen Quality ◽  
Rapid Test ◽  
Significant Negative Correlation ◽  
Fixed Time ◽  
Bovine Semen ◽  
The Mean

ABSTRACT The objective of the study was to determine whether or not there is a correlation between thermoresistance tests (TT) after semen thawing and pregnancy rate (PR) after fixed-time artificial insemination (FTAI). Four different TT were performed on ten samples used for AI; a rapid test (RTT) (30min / 46°C) and three slow tests (STT): STT1 (60min/38°C), STT2 (180min/38°C), and STT3 (300min/38°C). Two hundred and fifteen multiparous crossbred cows were submitted to FTAI under the following protocol: on day zero (d0) the animals received a P4 device +EB; on d7 PGF2α; on d8 P4 was removed and eCG+EC were administered; IATF was performed on d10. Three gestational diagnoses (G D) were performed on d40, d70 and d120. The mean sperm motility (%) in RTT and STTs were 19.84±6.13, 28.55±10.48, 17.62±5.87 and 8.63±3.46, respectively, and TP in the three DG 61.86%, 57.67%, and 55.81%, respectively. Through Person test a significant negative correlation (P< 0.05) was found between STT2 and PR at 60 days (r= -0.644) and between STT3 and all TPs (r= -0.774, -0.752, 0.748). It was concluded that TT parameters are not able to determine correlation between semen quality and TP.

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