General Taq PCR Master Mix -- CHEM 384/584 v2

2X PCR Master Mixes are convenient to use as they include all the necessary PCR components except the template and primers. Most PCR Master Mixes also include agarose gel running dyes and a density reagent that allows direct loading of PCR products on an agarose gel for electrophoresis. The master mix format simplifies workflows and sample handling; simply add primers, template, and water and then begin PCR.

General Taq PCR Master Mix -- CHEM 384/584 v2

SapphireAmp Fast PCR Master Mix contains a hot start PCR enzyme, optimized buffer, dNTP mixture, gel loading dye (blue), and a density reagent as a 2X premix. SapphireAmp Fast PCR Master Mix is optimized for fast PCR and offers a rapid extension rate (10 sec. per kb). The inclusion of blue dye and a density reagent allows direct loading of PCR products on an agarose gel for electrophoresis. The master mix format simplifies workflows and sample handling; simply add primers, template, and water and then begin PCR. SapphireAmp Fast PCR Master Mix is ideal for fast colony PCR screening. Fast colony PCR amplification of a 5 kb insert can be completed in approximately 1 hr 15 min. Furthermore, it is possible to amplify fragments up to 6 kb from genomic DNA templates.

IMPROVING THE EFFICIENCY OF DELIVERY OF PERISHABLE AGRICULTURAL CARGO IN INTERCITY TRANSPORTATION

The article analyzes the current state of the theory and practice of solving the issues related to the delivery of perishable goods by road. The author has determined that in order to improve the efficiency of cargo delivery, one of the promising directions is the development and application of transport delivery schemes, which take into account the change in consumer characteristics of perishable goods during transportation. Product losses during transportation must be taken into account when solving such problems as justifying the expediency of specialized rolling stock, choosing the method of transportation without transshipment, as well as when choosing the most rational delivery schemes. Losses of cargo are usually accounted for by the value of the actual losses and the value of the cargo. The article proposes the use of three schemes for the delivery of perishable cargo. The schemes differ in the way cargo is collected and the way goods are loaded into trucks. Also different are the types of vehicles that can be used in the delivery of goods under different schemes. The first scheme consists of the harvesting of peaches between the rows of the orchard by small tractors. The advantage of this scheme is the possibility of using heavy-duty vehicles with the achievement of maximum use of the capacity factor. Flatbed trucks are used for transportation. As a result of the accumulation of cargo at the point of loading, downtime is reduced by reducing the waiting time of cargo delivery. The second scheme is the need for in-line loading of vehicles in the fields of enterprises. Prefabricated routes for trucks and direct loading in the field are used. Application of the second scheme excludes the possibility of using heavy transport, because the movement will be made on unpaved roads. The need for in-line loading also eliminates the use of loading and unloading mechanisms. The third delivery scheme is similar to the first, but it is proposed to use specialized vehicles with a refrigerated chamber for transportation. As criterion of assessment of expediency of use of the offered schemes total expenses for cargo delivery are accepted taking into account possible losses from cargo damage, on the offered criterion it is expedient to estimate influence of such parameters, as volume of the cargo presented for transportation and length of the route.

Compaction and Breakage Characteristics of Crushed Stone Used as the Backfill Material of Urban Pavement Subsidence

The subsidence of urban pavement is becoming frequent accidents, and backfill is the primary means of remedy. Crushed stone is a commonly used aggregate for backfill material in engineering, and its compaction behavior under load needs to be well understood. In this work, a series of compaction tests were carried out on the same batch of crushed stone samples with the same gradation. The content changes of particles with different sizes were analyzed, the particle breakage characteristics during the compaction process were discussed, and the difference in particle breakage caused by loading speed and loading mode was examined. It shows the following: (1) For all samples, the content of particles that were crushed during compaction was always less than 40%. The particles with the strongest breakage varied with sample gradation. (2) The particle breakage could be divided into four categories: complete fragmentation, complete rupture, local fragmentation, and surface grinding. They affected the particle size distribution after compaction to varying degrees. (3) The particle breakage could be expressed as a cubical parabola of loading speed, whose coefficients are related to the sample gradation. (4) Stepwise loading rendered stronger particle breakage than direct loading, and the increase of particle breakage due to loading mode was more evident for continuous grading samples than discontinuous grading samples. This study will provide an experimental basis and reference for the selection and use of backfill aggregate in urban subsidence areas.

Interaction of the junction station of JSC «Russian railways» and non-public tracks of the oil refining complex

The article looks upon the features of interaction between the junction station of JSC «Russian Railways» and the tracks of non-public use of the oil refining complex. Technological maps of oil products loading are given, broken down into operations. The analysis of the main characteristics of the existing electric pumps of loading platforms is made with indication of types of cargo and selection of volumes of oil products loading on pointloading and gallery-type overpasses on certain days, taking into account loading in winter and summer periods. The order of loading time in winter and summer is determined. Based on the obtained data, a correlation analysis was performed to determine the statistical relationship between the two values. The identified dependencies allow the customer to predict and adjust production plans depending on the volume of loading by the nomenclature of oil cargo, the time of direct loading, the time of year, infrastructure factors and available technical means, and have reserves for improving the efficiency of interaction between the junction station and non-public tracks in terms of increasing the volume of loading of oil cargo in the future.

Direct loading of blood for plasma separation and diagnostic assays on a digital microfluidic device

The integration of 3D porous membranes in a digital microfluidic device enables the generation of cell-free plasma from finger-stick volumes of blood with in-line, on-chip analysis.

Deformation-Assisted Joining of Sheets to Tubes by Annular Sheet Squeezing

This paper is built upon the deformation-assisted joining of sheets to tubes, away from the tube ends, by means of a new process developed by the authors. The process is based on mechanical joining by means of form-fit joints that are obtained by annular squeezing (compression) of the sheet surfaces adjacent to the tubes. The concept is different from the fixing of sheets to tubes by applying direct loading on the tubes, as is currently done in existing deformation-assisted joining solutions. The process is carried out at room temperature and its development is a contribution towards ecological and sustainable manufacturing practices due to savings in material and energy consumption and to easier end-of-life disassembly and recycling when compared to alternative processes based on fastening, riveting, welding and adhesive bonding. The paper is focused on the main process parameters and special emphasis is put on sheet thickness, squeezing depth, and cross-section recess length of the punches. The presentation is supported by experimentation and finite element modelling, and results show that appropriate process parameters should ensure a compromise between the geometry of the mechanical interlocking and the pull-out strength of the new sheet–tube connections.

Design and Development of an Adaptive Bone Fracture Fixation System

The study aims to design and develop an Adaptive Bone Fracture Fixation System for the purpose of enhancing the control over fracture fixation as well as to permit quick device installation. The external ring fixator was designed to employ the use of bolted-nut hinges and rapid-gradual lengthening longitudinal elements to allow for full assembly expansion and progressive or immediate shape adjustment. The ring fixator was stress tested via direct loading (compression and tension) to determine if it can support the average human body weight [max 3.5 kN]. The study had verified that the device possesses intricate designs that would enhance the functionality of the external ring fixator, however these designs would limit the structural integrity of the bone fracture fixation system.