Direct loading of blood for plasma separation and diagnostic assays on a digital microfluidic device

Lab on a Chip ◽  
2020 ◽  
Vol 20 (10) ◽  
pp. 1845-1855 ◽  
Author(s):  
Christopher Dixon ◽  
Julian Lamanna ◽  
Aaron R. Wheeler
Keyword(s):  
Microfluidic Device ◽  
Porous Membranes ◽  
Plasma Separation ◽  
Diagnostic Assays ◽  
Direct Loading ◽  
On Chip ◽  
Finger Stick ◽  
Digital Microfluidic ◽  
Free Plasma ◽  
Chip Analysis

The integration of 3D porous membranes in a digital microfluidic device enables the generation of cell-free plasma from finger-stick volumes of blood with in-line, on-chip analysis.

scholarly journals An inkjet printed, roll-coated digital microfluidic device for inexpensive, miniaturized diagnostic assays

Lab on a Chip ◽  
2016 ◽  
Vol 16 (23) ◽  
pp. 4560-4568 ◽  
Author(s):  
Christopher Dixon ◽  
Alphonsus H. C. Ng ◽  
Ryan Fobel ◽  
Mark B. Miltenburg ◽  
Aaron R. Wheeler
Keyword(s):  
Microfluidic Device ◽  
Inkjet Printing ◽  
Mass Production ◽  
Roll Coating ◽  
Diagnostic Assays ◽  
Digital Microfluidic

Inkjet printing is combined with roll-coating to fabricate digital microfluidic (DMF) devices outside of the cleanroom for inexpensive, miniaturized diagnostic assays, with straightforward scalability towards mass production.

scholarly journals A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography

IUCrJ ◽  
2019 ◽  
Vol 6 (3) ◽  
pp. 454-464 ◽  
Author(s):  
Raphaël de Wijn ◽  
Oliver Hennig ◽  
Jennifer Roche ◽  
Sylvain Engilberge ◽  
Kevin Rollet ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Room Temperature ◽  
Optimal Conditions ◽  
General Applicability ◽  
Serial Data ◽  
Serial Crystallography ◽  
On Chip ◽  
Standard Techniques ◽  
Chip Analysis ◽  
Counter Diffusion

Determining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, a microfluidic device is described that enables the production of crystals by counter-diffusion and their direct on-chip analysis by serial crystallography at room temperature. Nine `non-model' and diverse biomacromolecules, including seven soluble proteins, a membrane protein and an RNA duplex, were crystallized and treated on-chip with a variety of standard techniques including micro-seeding, crystal soaking with ligands and crystal detection by fluorescence. Furthermore, the crystal structures of four proteins and an RNA were determined based on serial data collected on four synchrotron beamlines, demonstrating the general applicability of this multipurpose chip concept.

On-chip organic synthesis enabled using an engine-and-cargo system in an electrowetting-on-dielectric digital microfluidic device

Lab on a Chip ◽  
2019 ◽  
Vol 19 (18) ◽  
pp. 3054-3064 ◽  
Author(s):  
Matin Torabinia ◽  
Parham Asgari ◽  
Udaya Sree Dakarapu ◽  
Junha Jeon ◽  
Hyejin Moon
Keyword(s):  
Organic Synthesis ◽  
Chemical Reaction ◽  
Microfluidic Device ◽  
Electrowetting On Dielectric ◽  
On Chip ◽  
Digital Microfluidic

This paper presents a microfluidic chemical reaction using an electrowetting-on-dielectric (EWOD) digital microfluidic device.

Analytical Models to Determine the Electric Field Characteristics of a Multi-Electrode Impedimetric Immunosensor in a Digital Microfluidic Device

2014 ◽  
Author(s):  
Steffen O. P. Blume ◽  
Michael J. Schertzer ◽  
Ridha Ben Mrad ◽  
Pierre E. Sullivan
Keyword(s):  
Microfluidic Device ◽  
Digital Microfluidics ◽  
Electrode Array ◽  
Analytical Models ◽  
Element Analysis ◽  
Numerical Simulation Methods ◽  
Mapping Techniques ◽  
On Chip ◽  
Digital Microfluidic ◽  
Impedimetric Immunosensor

The level of integration of digital microfluidics is continually increasing to include the system path from fluid manipulation and transport, on to reagent preparation, and finally reaction detection. Digital microfluidics therefore has the capability to encompass all steps of common biochemical protocols. Reported here is a set of analytical models for the design of a coplanar interdigitated multi-electrode array to be used as an impedimetric immunosensor in a digital microfluidic device for on-chip chemical reaction detection. The models are based on conformal mapping techniques, and are compared to results obtained from finite element analysis to discuss limitations of the model. The analytical models are feasible and inexpensive surrogates for numerical simulation methods.

scholarly journals On-chip characterization of cryoprotective agent mixtures using an EWOD-based digital microfluidic device

Lab on a Chip ◽  
2011 ◽  
Vol 11 (13) ◽  
pp. 2212 ◽  
Author(s):  
Sinwook Park ◽  
Pavithra A. L. Wijethunga ◽  
Hyejin Moon ◽  
Bumsoo Han
Keyword(s):  
Microfluidic Device ◽  
Cryoprotective Agent ◽  
On Chip ◽  
Digital Microfluidic

scholarly journals Integrating Biosensors in Organs-on-Chip Devices: A Perspective on Current Strategies to Monitor Microphysiological Systems

Biosensors ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 110 ◽  
Author(s):  
Erika Ferrari ◽  
Cecilia Palma ◽  
Simone Vesentini ◽  
Paola Occhetta ◽  
Marco Rasponi
Keyword(s):  
Imaging Techniques ◽  
Biological Models ◽  
Electromechanical Properties ◽  
Human Organs ◽  
Tissue Constructs ◽  
Microphysiological Systems ◽  
Metabolic Activities ◽  
On Chip ◽  
Chip Analysis

Organs-on-chip (OoC), often referred to as microphysiological systems (MPS), are advanced in vitro tools able to replicate essential functions of human organs. Owing to their unprecedented ability to recapitulate key features of the native cellular environments, they represent promising tools for tissue engineering and drug screening applications. The achievement of proper functionalities within OoC is crucial; to this purpose, several parameters (e.g., chemical, physical) need to be assessed. Currently, most approaches rely on off-chip analysis and imaging techniques. However, the urgent demand for continuous, noninvasive, and real-time monitoring of tissue constructs requires the direct integration of biosensors. In this review, we focus on recent strategies to miniaturize and embed biosensing systems into organs-on-chip platforms. Biosensors for monitoring biological models with metabolic activities, models with tissue barrier functions, as well as models with electromechanical properties will be described and critically evaluated. In addition, multisensor integration within multiorgan platforms will be further reviewed and discussed.

scholarly journals Design and Manufacturing of a Disposable, Cyclo-Olefin Copolymer, Microfluidic Device for a Biosensor †

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 1178 ◽  
Author(s):  
Jorge Prada ◽  
Christina Cordes ◽  
Carsten Harms ◽  
Walter Lang
Keyword(s):  
Microfluidic Device ◽  
Heating Temperature ◽  
Low Cost ◽  
Reaction Chamber ◽  
Microfluidic System ◽  
Heating Process ◽  
Design And Manufacturing ◽  
On Chip ◽  
Working Parameters ◽  
Auto Fluorescence

This contribution outlines the design and manufacturing of a microfluidic device implemented as a biosensor for retrieval and detection of bacteria RNA. The device is fully made of Cyclo-Olefin Copolymer (COC), which features low auto-fluorescence, biocompatibility and manufacturability by hot-embossing. The RNA retrieval was carried on after bacteria heat-lysis by an on-chip micro-heater, whose function was characterized at different working parameters. Carbon resistive temperature sensors were tested, characterized and printed on the biochip sealing film to monitor the heating process. Off-chip and on-chip processed RNA were hybridized with capture probes on the reaction chamber surface and identification was achieved by detection of fluorescence tags. The application of the mentioned techniques and materials proved to allow the development of low-cost, disposable albeit multi-functional microfluidic system, performing heating, temperature sensing and chemical reaction processes in the same device. By proving its effectiveness, this device contributes a reference to show the integration potential of fully thermoplastic devices in biosensor systems.

scholarly journals Microsphere-Based Microfluidic Device for Plasma Separation and Potential Biochemistry Analysis Applications

Micromachines ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 487
Author(s):  
Hongyan Xu ◽  
Zhangying Wu ◽  
Jinan Deng ◽  
Jun Qiu ◽  
Ning Hu ◽  
...  
Keyword(s):  
Blood Sample ◽  
Microfluidic Device ◽  
Biochemical Analysis ◽  
Cost Effective ◽  
Clinical Diagnostics ◽  
Correction Factors ◽  
Separation Device ◽  
Blood Samples ◽  
Plasma Separation ◽  
Blood Biochemical

The development of a simple, portable, and cost-effective plasma separation platform for blood biochemical analysis is of great interest in clinical diagnostics. We represent a plasma separation microfluidic device using microspheres with different sizes as the separation barrier. This plasma separation device, with 18 capillary microchannels, can extract about 3 μL of plasma from a 50 μL blood sample in about 55 min. The effects of evaporation and the microsphere barrier on the plasma biochemical analysis results were studied. Correction factors were applied to compensate for these two effects. The feasibility of the device in plasma biochemical analysis was validated with clinical blood samples.

Electrowetting-on-dielectric (EWOD) digital microfluidic device for in-line workup in organic reactions: A critical step in the drug discovery work cycle

2021 ◽  
Vol 330 ◽  
pp. 129252
Author(s):  
Matin Torabinia ◽  
Udaya Sree Dakarapu ◽  
Parham Asgari ◽  
Junha Jeon ◽  
Hyejin Moon
Keyword(s):  
Drug Discovery ◽  
Microfluidic Device ◽  
Organic Reactions ◽  
Electrowetting On Dielectric ◽  
Critical Step ◽  
Work Cycle ◽  
Digital Microfluidic

A Microfluidic Platform for On-Chip Analysis of Circulating Tumor Cells

2021 ◽  
Author(s):  
Jeff Darabi ◽  
Joseph Schober
Keyword(s):  
Cancer Cells ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Whole Blood ◽  
Peripheral Blood ◽  
Automated Image Analysis ◽  
Rare Cancer ◽  
On Chip ◽  
Chip Analysis ◽  
Selection Of

Abstract Studies have shown that primary tumor sites begin shedding cancerous cells into peripheral blood at early stages of cancer, and the presence and frequency of circulating tumor cells (CTCs) in blood is directly proportional to disease progression. The challenge is that the concentration of the CTCs in peripheral blood may be extremely low. In the past few years, several microfluidic-based concepts have been investigated to isolate CTCs from whole blood. However, these devices are generally hampered by complex fabrication processes and very low volumetric throughputs, which may not be practical for rapid clinical applications. This paper presents a high-performance yet simple magnetophoretic microfluidic chip for the enrichment and on-chip analysis of rare CTCs from blood. Microscopic and flow cytometric assays developed for selection of cancer cell lines, selection of monoclonal antibodies, and optimization of bead coupling are discussed. Additionally, on-chip characterization of rare cancer cells using high resolution immunofluorescence microscopy and modeling results for prediction of CTC capture length are presented. The device has the ability to interface directly with on-chip pre and post processing modules such as mixing, incubation, and automated image analysis systems. These features will enable us to isolate rare cancer cells from whole blood and detect them on the chip with subcellular resolution.

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