RANCANG BANGUN KONTROL KADAR AMONIA DAN PH AIR BERBASIS ARDUINO PADA BUDIDAYA IKAN

In the fish pond ecosystem there are various factors that can inhibit fish growth or even kill fish, one of which is the water factor, the water itself has various factors, one of which is the quality of PH levels and amonia levels. Therefore, this paper discusses the design and manufacture of a control device for PH levels and amonia levels to make it easier for farmers to check PH levels and amonia levels in the pond. This control device uses a DFROBOT V.2 sensor as a PH sensor and a DS18B20 sensor as a temperature sensor as well as an Arduino UNO controller. The method used to control amonia is by comparing the data from PH and the temperature in the pool water, this tool will work if the amonia level is higher. from 0.8ppm by activating the aerator to keep the amonia in the pool water normal. Meanwhile, to maintain the PH of the water more than 6 and less than 7, it is necessary to add pond water by activating the water pump. The results of this study can be used as a reference for future research for the development of Arduino Uno-based PH and amonia level control devices.

Environmental Water and Sediment Microbial Communities Shape Intestine Microbiota for Host Health: The Central Dogma in an Anthropogenic Aquaculture Ecosystem

From increasing evidence has emerged a tight link among the environment, intestine microbiota, and host health status; moreover, the microbial interaction in different habitats is crucial for ecosystems. However, how the environmental microbial community assembly governs the intestinal microbiota and microbial communities of multiple habitats contribute to the metacommunity remain elusive. Here, we designed two delicate experiments from temporal and spatial scales in a shrimp culture pond ecosystem (SCPE). Of the SCPE metacommunity, the microbial diversity was mainly contributed to by the diversity of–βIntraHabitats and βInterHabitats, and water and sediment communities had a large contribution to the shrimp intestine community as shown by SourceTracker and Sloan neutral community model analyses. Also, phylogenetic bin-based null model results show that microbial assembly of three habitats in the SCPE appeared to be largely driven by stochastic processes. These results enrich our understanding of the environment–intestinal microbiota–host health closely linked relationship, making it possible to be the central dogma for an anthropogenic aquaculture ecosystem. Our findings enhance the mechanistic understanding of microbial assembly in the SCPE for further analyzing metacommunities, which has important implications for microbial ecology and animal health.

Niche Preference of Escherichia coli in a Peri-Urban Pond Ecosystem

Escherichia coli comprises diverse strains with a large accessory genome, indicating functional diversity and the ability to adapt to a range of niches. Specific strains would display greatest fitness in niches matching their combination of phenotypic traits. Given this hypothesis, we sought to determine whether E. coli in a peri-urban pond and associated cattle pasture display niche preference. Samples were collected from water, sediment, aquatic plants, water snails associated with the pond, as well as bovine feces from cattle in an adjacent pasture. Isolates (120) were obtained after plating on Membrane Lactose Glucuronide Agar (MLGA). We used the uidA and mutS sequences for all isolates to determine phylogeny by maximum likelihood, and population structure through gene flow analysis. PCR was used to allocate isolates to phylogroups and to determine the presence of pathogenicity/virulence genes (stxI, stxII, eaeA, hlyA, ST, and LT). Antimicrobial resistance was determined using a disk diffusion assay for Tetracycline, Gentamicin, Ciprofloxacin, Meropenem, Ceftriaxone, and Azithromycin. Our results showed that isolates from water, sediment, and water plants were similar by phylogroup distribution, virulence gene distribution, and antibiotic resistance while both snail and feces populations were significantly different. Few of the feces isolates were significantly similar to aquatic ones, and most of the snail isolates were also different. Population structure analysis indicated three genetic backgrounds associated with bovine, snail, and aquatic environments. Collectively these data support niche preference of E. coli isolates occurring in this ecosystem.

Effect of Vegetable Growth on Content and Composition of Antibiotics in Litopenaeus vannamei Pond Sediments in Crop/Aquacultural Rotation Process

Photodegradation remains the major pathway of antibiotic removal in natural ponds. This study introduced a new method of growing vegetables on the bottom substrate of shrimp ponds to improve sediment quality. The aim of the study was to investigate the effect of vegetable planting on the photodegradation of antibiotics. This study characterized antibiotic levels in the pond sediment during this phytoremediation process and investigated the antibiotic content and composition of the sediment with and without crop rotation (traditional control), as well as the shrimp yields. The results showed that total antibiotics (e.g., trimethoprim, oxytetracycline, and norfloxacin) in the sediment of all aquaculture ponds continuously decreased from 44.78 ± 4.07 μg/kg to 18.80 ± 2.26 μg/kg in the crop rotation pond. The total amount of antibiotics consistently decreased in all ponds, and the rate of decline did not greatly differ. However, oxytetracycline in the crop rotation pond decreased faster than in the control pond, presumably because the growing vegetables altered the sediment and microbial-community characteristics that promoted oxytetracycline degradation. In the following year, there was little difference in the levels of norfloxacin or oxytetracycline between the two ponds. An increase in trimethoprim in the control pond was much higher than in the crop-growing sediment. It was indicated that the system remediated the shrimp pond ecosystem as well as providing the possibility of increasing profits by planting vegetables in the winter idle period of shrimp ponds.

Ecological engineering approaches to restoring the aquatic biological community of an urban pond ecosystem and its effects on water quality ‑ a case study of the urban Xixi National Wetland Park in China

There is a global increase in urbanization, which contributes to the loss and fragmentation of urban wetland and pond habitats. Urban ponds have an important role in the ecology of urban areas, as they provide essential habitats for aquatic species. The objective of this study is to demonstrate ecological engineering approaches to restoring aquatic biological communities in an urban pond ecosystem, which can be used to achieve water purification of such ecosystems globally. The general approach of first cleaning out the ponds and then systematically applying different treatments of plants and fish was designed and implemented in the field. We used three replicated ponds per engineering treatment to determine which of the treatments was the best option based on an assessment of water quality and biological indicators. The main findings of this study were as follows: a combination of aquatic animals and aquatic plants can provide the best water purification performance; furthermore, macrobenthos, such as Bellamya purificata, can be used as biological indicator species for monitoring dominant species and water quality in a local urban pond ecosystem. In conclusion, maintaining biological species diversity over different trophic levels can provide the best water purification performance in urban pond ecosystems.