Biphalin—A Potent Opioid Agonist—As a Panacea for Opioid System-Dependent Pathophysiological Diseases?

Biphalin, one of the opioid agonists, is a dimeric analog of enkephalin with a high affinity for opioid receptors. Opioid receptors are widespread in the central nervous system and in peripheral neuronal and non-neuronal tissues. Hence, these receptors and their agonists, which play an important role in pain blocking, may also be involved in the regulation of other physiological functions. Biphalin was designed and synthesized in 1982 by Lipkowski as an analgesic peptide. Extensive further research in various laboratories on the antinociceptive effects of biphalin has shown its excellent properties. It has been demonstrated that biphalin exhibits an analgesic effect in acute, neuropathic, and chronic animal pain models, and is 1000 times more potent than morphine when administered intrathecally. In the course of the broad conducted research devoted primarily to the antinociceptive effect of this compound, it has been found that biphalin may also potentially participate in the regulation of other opioid system-dependent functions. Nearly 40 years of research on the properties of biphalin have shown that it may play a beneficial role as an antiviral, antiproliferative, anti-inflammatory, and neuroprotective agent, and may also affect many physiological functions. This integral review analyzes the literature on the multidirectional biological effects of biphalin and its potential in the treatment of many opioid system-dependent pathophysiological diseases.

Empathy Levels Among Veterinary Medicine Students in Colombia (South America)

Empathy plays an important role in veterinarians’ relationships with their patients, clients, and colleagues. Because it relates to greater clinical competence and facilitates the acquisition of information for diagnosing, prescribing therapies, and identifying and treating animal pain, empathy is an essential competence to be strengthened during professional training. The objective of this study was to evaluate the empathy levels of veterinary medicine students toward people and animals and to identify associated factors. The animal empathy scale and the Davis interpersonal reactivity index were applied through an electronic survey to first-, third-, and fifth-year students ( n = 559) in three veterinarian medical schools in Colombia. A principal components analysis was performed to identify composite scores of human and animal empathy levels. The empathy toward humans total score ranged from 0 to 112, and the empathy toward animals total score was between 22 and 198. The average empathy scores for students were 89.67 ± 9.02 (mean ± SD; range: 60–115) and 115.01 ± 13.41 (mean ± SD; range: 67–165), respectively. The results suggest that empathy scores toward people are acceptable. Gender, university, program type, age, year of study, and diet were significantly associated with empathy levels toward animals. It is proposed that levels of empathy toward animals be strengthened by fostering a positive learning environment, developing ethical and animal welfare competencies, and increasing empathetic contact and hands-on experience with animals during the curriculum.

Antinociceptive Synergism of Pomegranate Peel Extract and Acetylsalicylic Acid in an Animal Pain Model

Several modern drugs, which are derived from traditional herbal medicine are used in contemporary pharmacotherapy. Currently, the study of drug–plant interactions in pain has increased in recent years, looking for greater efficacy of the drug and reduce side effects. The antinociception induced by intragastric co-administration of the combination of pomegranate peel extract (PoPEx) and acetylsalicylic acid (ASA) was assessed using the isobolographic analysis in formalin test (nociceptive and inflammatory pain). The effective dose that produced 30% of antinociception (ED30) was calculated for both drugs from the logarithmic dose–response curves, subsequently generating a curve with the combination on fixed proportions (1:1) of PoPEx and ASA. Through isobolographic analysis, this experimental ED30 was compared with the calculated theoretical additive ED30. The result was a synergistic interaction, the experimental ED30 was significantly smaller (p < 0.05) than the theoretical ED30. The antinociceptive mechanism of the PoPEx-ASA combination involves the l-Arginine/NO/cGMP pathway, antioxidant capacity, and high content of total phenols. These findings suggest that an interaction between PoPEx and ASA could be a novel treatment for inflammatory and nociceptive pain, also diminish the secondary reactions of ASA.

Do we Need a Plant Theodicy?

In recent decades, philosophers and theologians have become increasingly aware of the extent of animal pain and suffering, both past and present, and of the challenge this poses to God’s goodness and justice. As a result, a great deal of effort has been devoted to the discussion and development of animal theodicies, that is, theodicies that aim to offer morally sufficient reasons for animal pain and suffering that are in fact God’s reasons. In this paper, I ask whether there is a need to go even further than this, by considering whether effort should be made to extend theodicy to include plants as well. Drawing upon ideas found in some recent animal theodicies as well as in the work of some environmental ethicists, I offer three arguments for supposing that plants should indeed fall within the purview of theodicy: (1) the argument from non-flourishing as evil, (2) the argument from moral considerability, and (3) the argument from intrinsic value. I also consider a possible objection to each of these arguments. Having outlined and defended the aforementioned arguments for broadening theodicy to include plants as well as humans and animals, I conclude by considering what a plant theodicy might look like.

The Topic of the Ideal Dairy Farm Can Inspire How to Assess Knowledge about Dairy Production Processes: A Case Study with Students and Their Contributions

The dairy farm and on-farm production processes are the subject of numerous evaluations. These are not only evaluations of the economic efficiency of milk production. Opinions expressed by various social groups are also an important contribution to improving the approach to milk production on the farm. As a result of such opinions, a vision of an ideal dairy farm may be formed. The aim of the study was to develop the thematic area of an ideal dairy farm in the opinion of two groups of students who were learning remotely (in the distance learning system) due to the Covid-19 pandemic. The first group consisted of six Erasmus+ students. The second group consisted of 70 full-time Polish students. As part of their homework, the students answered three questions about the ideal dairy farm. Students had 4 weeks to do their homework. Erasmus+ students’ homework was used to propose a ranking method for assessing the answers to three questions by the students themselves. Homework of Polish students was used to analyze the frequency of using certain keywords. Polish students, in their homework on the ideal dairy farm, mainly used the basic concepts related to dairy production. Unfortunately, a very small number of students used terms that represent a responsible approach to dairy production, including ethical aspects, freedom, sustainability, animal pain, antibiotics, and organic milk production. In conclusion, it was indicated that the curriculum should be structured in such a way as to raise students’ awareness of dairy production and its current challenges.

Chemokines and Pain in the Trigeminal System

Chemotactic cytokines or chemokines are a large family of secreted proteins able to induce chemotaxis. Chemokines are categorized according to their primary amino acid sequence, and in particular their cysteine residues that form disulphide bonds to maintain the structure: CC, CXC, CX3C, and XC, in which X represents variable amino acids. Among their many roles, chemokines are known to be key players in pain modulation in the peripheral and central nervous systems. Thus, they are promising candidates for novel therapeutics that could replace current, often ineffective treatments. The spinal and trigeminal systems are intrinsically different beyond their anatomical location, and it has been suggested that there are also differences in their sensory mechanisms. Hence, understanding the different mechanisms involved in pain modulation for each system could aid in developing appropriate pharmacological alternatives. Here, we aim to describe the current landscape of chemokines that have been studied specifically with regard to trigeminal pain. Searching PubMed and Google Scholar, we identified 30 reports describing chemokines in animal models of trigeminal pain, and 15 reports describing chemokines involved in human pain associated with the trigeminal system. This review highlights the chemokines studied to date at different levels of the trigeminal system, their cellular localization and, where available, their role in a variety of animal pain models.

Analgesic Interaction Between Morphine with Fentanyl In Experimental Murine Pain

Opioids are among the most effective pain relievers available, however multimodal antinociception between opioids, has not been extensively studied in diverse animal pain models.In this study the pharmacological interaction of morphine with fentanyl was evaluated in different murine pain models by means of isobolographic analysis. In control animals, morphine and fentanyl produced a dose-related antinociceptive action in the murine assays and the rank of potency was: formalin hind paw phase I > formalin phase II > tail flick. Coadministration of morphine with fentanyl, in a fixed relation 1:1 of their ED50, produces a dose response in all tests and the isobologram resulted in synergism. Fentanyl was more effective than morphine which could be explained according the suggestion that opioids could be acting through other targets, with different binding capacity thru the regulation or activation of non-opioid receptors. Co-administration of morphine with fentanyl induces synergism in all murine trials, confirming the antinociceptive capacity of both opioids which would constitute a promisory idea to multimodal treatment of pain.