The study aimed to examin the role of human resources information systems in their dimensions (Training Information Systems, Performance Appraisal Information Systems, Employment Information Systems, Incentive Information Systems, HRIS Information Systems), in enforacing knowledge management in its dimensions, (Knowledge generation, knowledge storage, knowledge dissemination, knowledge application) in the Ministry of Transport in the Gaza Strip. The researchers used the descriptive analytical method. The study population consisted of all employees in supervisory positions in the Minstry of Transport. The questionnaire was used to collect the data required data. The number of respondents was of 62 respondents with a recovery rate (82.3%). Data was analyzed using SPSS statistical program. The study showed that there is a statistically significant relationship between the application level of human resources information systems and knowledge management application in the Minstry of Transport. In addition, the application level of human resources information systems impacts the ?application level of knowledge management at a rate of (83.40%).