A Ten-Week Motor Skills Training Program Increases Motor Coordination in Children with Developmental Coordination Disorder

The present study aimed to investigate the effect of a motor skills training program in children with DCD considering their gender. The Movement Assessment Battery for Children (MABC-2) classified the children and assessed skill changes over time. The study was implemented at four kindergartens in the Khorezm region of Uzbekistan. In the study, all children had DCD (5.17 ± 0.70 years; 10 girls), and all indicators on MABC-2 were less than 16 percent. Participants were divided into an experimental group (n=17) receiving ten weeks of motor skills training program for 45 min twice per week, the control group (n=7) proceeded with exercises of everyday living. All children in the experimental group had a significant increase in total percentile rank of MABC-2 and concerning each domain (manual dexterity; throwing and catching; balance). In the control group, a significant decrease in the total percentile rank of MABC-2 and each domain of MABC-2 was observed. The effectiveness of the intervention program was similar across both genders. The study supports that a period of 10 weeks of a motor skills training program can increase the quality of children's motor coordination and represent a valuable procedure for physical education specialists to enhance motor skills for children with DCD.

Does it pay to Play by the Rules? Respect for Rule of law, Control of Corruption, and National Success at the Summer Olympics

Utilizing the set of World Governance Indicators published by the World Bank, this paper finds that scoring highly in an indicator measuring respect for rule of law and control of corruption is associated with fewer athletes disqualified and higher medal shares at the Summer Olympics from 1996–2016. Notable reductions in disqualifications and increases in medal shares occur at coincident percentile ranks in the aforementioned indicator, with nations at the 67th percentile rank and above having a 13.8% higher probability of medaling and a 12.11% lower probability of having an athlete disqualified. These results uncover a new link between governance and Olympic success and provide support for the existing anti-doping rules and enforcement as, ceteris paribus, it would seem that nations whose athletes respect and abide by the rules achieve higher medal shares than those whose athletes do not.

Sistem Pemilihan Bidang Minat Mahasiswa Menggunakan Metode Profile Matching Berbasis Web

Penelitian ini mengusulkan tentang sistem pendukung keputusan pemilihan bidang minat mahasiswa dibuat untuk membantu menyelesaikan masalah mahasiswa dalam menentukan peminatannya dalam bidang informatika. Metode Profile Matching dipilih karena mampu menyeleksi bidang minat yang sesuai dengan bobot kriteria yang dimiliki oleh mahasiswa. Implementasi web sistem pendukung keputusan ini menggunakan bahasa pemrograman HTML, PHP murni dan menggunakan Database MySQL. Untuk membuat tampilan web yang lebih menarik, digunakan boostrap dan javascript yang nantinya semua data aplikasi disimpan di web server penyedia hosting. Pengujian aplikasi oleh responden 10 mahasiswa dengan usability testing menggunakan Standardized Universal Percentile Rank-Questionnare (SUPR-Q) untuk user dan admin serta pengujian menggunakan User Acceptance Test (UAT). Kesimpulan akhir didapatkan kesesuaian antara bidang minat yang dipilih oleh mahasiswa dengan hasil yang direkomendasikan oleh sistem dengan persentase sebesar 70%.

Subseasonal Forecasts of the Northern Queensland Floods of February 2019: Causes and Forecast Evaluation

During the austral summer 2018/19, devastating floods occurred over northeast Australia that killed approximately 625,000 head of cattle and inundated over 3000 homes in Townsville. In this paper, the disastrous event was identified as a record-breaking subseasonal peak rainfall event (SPRE). The SPRE was mainly induced by an anomalously strong monsoon depression that was modulated by the convective phases of an MJO and an equatorial Rossby (ER) wave. The ER wave originated from an active equatorial deep convection associated with the El Niño warm sea surface temperatures near the dateline over the central Pacific. Based on the S2S Project Database, we analyzed the extended-range forecast skill of the SPRE from two different perspectives, the monsoon depression represented by an 850-hPa wind shear index and the 15-day accumulated precipitation characterized by the percentile rank (PR) and the ratio to the three-month seasonal (DJF) totals. The results of four S2S models of this study suggest that the monsoon depression can maintain the same level of skill as the short-range (3 days) forecast up to 8–10 days. For precipitation parameters, the conclusions are similar to the monsoon depression. For the 2019 northern Queensland SPRE, the model forecast was, in general, worse than the expectation derived from the hindcast analysis. The clear modulation of the ER wave that enhanced the SPRE monsoon depression circulation and precipitation is suspected as the main cause for the lower forecast skill. The analysis procedure proposed in this study can be applied to analyze the SPREs and their associated large-scale drivers in other regions.

Improving tumor mutational burden calibration in non-European patients.

1559 Background: Tumor mutational burden (TMB), has been recently granted FDA approval as a biomarker for ICI treatment in tumors with a high mutation load (≥ 10/MB). To leverage this biomarker in the clinical setting, it is necessary to evaluate its application in diverse patient populations. In particular, tumor-only sequencing may overestimate TMB in non-EUR populations for which reference panels are small or unavailable, and thus have poorer predictive performance. Herein, we investigate the effect of TMB overestimation in non-EURs on patient diagnosis and clinical outcomes in a real world patient cohort. Methods: TMB was computed using a tumor-only NGS platform (Oncopanel) for 8349 cancer patients (pts) of 7 cancer types (Table). Genetic ancestry was inferred directly from tumor sequencing data and confirmed for a subset of pts using germline SNP arrays. TMB was compared using Wilcoxon rank-sum test between European (EUR) and non-EUR pts. TMB percentile rank by ancestral group (East Asian, African, European) and tumor histology was computed. In non-EUR pts, TMB was calibrated by reassigning its value to the corresponding percentile rank in EUR pts having the same cancer (AH-TMB). Tumors with raw TMB ≥ 10/MB were assigned as TMB-high (TMBH; with the rest referred to as TMBL for TMB-low) and those with calibrated AH-TMB ≥ 10/MB as AH-TMBH. A subset of pts treated with ICIs at DFCI and of EUR ancestry were analyzed. TMB was intentionally mis-calibrated in EURs (MC-TMB), to mimic the TMB overestimation observed in non-EURs. Associations between TMBH status and overall survival (OS) was assessed using Cox regression. Results: Uncalibrated TMB was significantly higher in tumors from non-EUR pts overall (p <0.0001, Table) as anticipated, whereas this difference was not observed in tumors with matched germline filtering. We reassigned non-EUR pts into low/high groups using calibrated AH-TMB, which changed the assignment of 67/670 non-EURs, most of which (65/67) were downgraded from TMBH to AH-TMBL. In the ICI-treated cohort, there was a strong association between TMBH status and OS (p = 2e-11, HR = 0.6) whereas MC-TMBH (mimicking the miscalibration in non-EUR samples) had a weaker association with OS (p = 0.0013, HR = 0.8). Importantly, pts assigned to TMB-H due to miscalibration (90 TMB-L/MC-TMBH pts) had shorter OS compared to the true TMBH group (p = 3e-6, HR = 1.9). Conclusions: In this analysis, we showed that tumor-only sequencing platforms can overestimate TMB in non-EUR pts, impacting treatment decisions and outcomes. Replacing raw TMB counts with an ancestry-adjusted measure could optimize TMB application in the clinical setting when germline sequencing is not available. Accurate ancestry inference can be performed using tumor-only sequencing.[Table: see text]

Rate-enhancing Single Amino Acid Mutation for Hydrolases: A Statistical Profiling

We reported the statistical profiling for rate-enhancing mutant hydrolases with single amino acid substitution. We constructed an integrated structure-kinetics database, IntEnzyDB, which contains 3,907 experimentally characterized hydrolase kinetics and 2,715 hydrolase Protein Data Bank IDs. The hydrolase kinetics data involve 9% rate-enhancing mutations. Mutation to nonpolar residues with a hydrocarbon chain shows a stronger preference for rate acceleration than to polar or charged residues. To elucidate the structure-kinetics relationship for rate-enhancing mutations, we categorized each mutation into one of the three spatial shells of hydrolases. We defined the spatial shells by reference to either the active site or the center-of-mass of the enzyme. In either case, mutations in the first shell (i.e., closest to the reference point) appear on average more rate-deleterious than those in the other two shells (i.e., ~1.0 kcal/mol in ∆∆G‡ ). Under the active-site reference, mutations in the third shell (i.e., most distal to the active site) exhibit the highest likelihood of rate enhancement. This propensity is significant for larger-sized hydrolases. In contrast, under the center-of-mass reference, mutations in the second shell (i.e., 33.3th to 66.7th percentile rank of spatial proximity to the center-of-mass of the enzyme) show the highest likelihood of rate enhancement. This trend is significant for smaller-sized hydrolases. The studies reveal the statistical features for identifying rate-enhancing mutations in hydrolases, which will potentially guide hydrolase discovery in biocatalysis.

Rate-enhancing Single Amino Acid Mutation for Hydrolases: A Statistical Profiling

We reported the statistical profiling for rate-enhancing mutant hydrolases with single amino acid substitution. We constructed an integrated structure-kinetics database, IntEnzyDB, which contains 3,907 experimentally characterized hydrolase kinetics and 2,715 hydrolase Protein Data Bank IDs. The hydrolase kinetics data involve 9% rate-enhancing mutations. Mutation to nonpolar residues with a hydrocarbon chain shows a stronger preference for rate acceleration than to polar or charged residues. To elucidate the structure-kinetics relationship for rate-enhancing mutations, we categorized each mutation into one of the three spatial shells of hydrolases. We defined the spatial shells by reference to either the active site or the center-of-mass of the enzyme. In either case, mutations in the first shell (i.e., closest to the reference point) appear on average more rate-deleterious than those in the other two shells (i.e., ~1.0 kcal/mol in ∆∆G‡ ). Under the active-site reference, mutations in the third shell (i.e., most distal to the active site) exhibit the highest likelihood of rate enhancement. This propensity is significant for larger-sized hydrolases. In contrast, under the center-of-mass reference, mutations in the second shell (i.e., 33.3th to 66.7th percentile rank of spatial proximity to the center-of-mass of the enzyme) show the highest likelihood of rate enhancement. This trend is significant for smaller-sized hydrolases. The studies reveal the statistical features for identifying rate-enhancing mutations in hydrolases, which will potentially guide hydrolase discovery in biocatalysis.

Immunoinformatic Responses of Host MHC I/II and CD4+ Cells Against Conserved Spike Fragments of SARS CoV-2 of 186 Countries With In-Silico Mutations: Implications in Universal Vaccination

Different types of quickly-developed vaccines have been introduced against Covid-19 with largely inconclusive results. On the course of time, somewhere Covid-19 declines its infection/mortality rate and in some countries it revived with some new mutant-variants. Considering the large number of global variants, in the current study several conserved (186 countries) sequences (checked by ClustalX2) epitopic regions (by SVMTriP and IEDB) and in-silico mutants of SARS CoV-2 spike protein fragments (Cut 1-4) were screened for their stability against proteases, antigenicity (VaxiJen V2.0 and for glycosylation effects NetOGlyc-NetNGlyc), MHCI/II reactivity (IEDB TOOLS) and CD4+ cellular responses utilizing Haddock 2.4 and PatchDock programme. The cut4 mutant and its peptide SRLFRKSNLKPFERD showed highest combined-score 48.23548 and Immunogenicity-Score of 92.0887. The core-sequence SRLFRKSNL showed highest Median Percentile Rank (7 HLA-allele) of 19. CD4+ immunogenicity also confirms representation of CUT4TM2 epitope SRLFRKSNL by MHC Class II. The epitope YNYKYRLFR from CUT4 showed IC50 value of ~30 nM with allele HLA-DRB1*11:01 and HLA-DRB5*01:01. Binding affinity and RMSD score suggest that different epitopic regions of Cut4 mutants interacted MHC II with large number of H-bonds. Cut4 double mutants strongly interacted with exposed T-cell surface and facilitated by its receptors. According to the antigenicity analysis, epitopes 3, 4, and 5 were the potent antigens with antigenicity values of 0.6268, 1.2404, and 0.4639, respectively. Screening of conserved SARS CoV-2 spike fragments globally may help to find most stable antigenic determinant and further their mutation-engendered counterparts have better immunogenic responses. Further studies are necessary to develop global vaccination strategies against Covid-19.

Evaluasi Usability pada Aplikasi Mobile ACC.ONE menggunakan System Usability Scale (SUS) dan Usability Testing

Perusahaan pembiayaan kendaraan mobil saat ini semakin banyak dan berkembang begitu pesat. Setiap perusahaan berusaha untuk menarik perhatian pelanggan dengan berbagai cara, terutama peningkatan teknologi untuk mendukung dan memudahkan proses kegiatan pembiayaan mobil. Perusahaan Astra Credit Company memanfaatkan teknologi dengan menyediakan aplikasi mobile acc.one yang dapat digunakan oleh pelanggan untuk membantu segala kegiatan proses pembiayaan mobil di Perusahaan ACC. Namun aplikasi ini terdapat beberapa masalah yang dapat diketahui dari ulasan pengguna di play store seperti aplikasi tiba-tiba error, aplikasi tidak dapat dibuka, fitur tidak berfungsi dengan semestinya, dll. Sehingga dilakukan pengujian aplikasi untuk mengetahui tingkat usability aplikasi dengan menggunakan metode system usability scale dan usability testing untuk mengetahui pengalaman pengguna. Hasil yang diperoleh tingkat usability aplikasi dengan tingkat kemudahan sebesar 73,33% yang masuk dalam kategori buruk, tingkat kecepatan pengguna sebesar 0,0467 goals/sec yang termasuk kategori normal, tingkat kesalahan pengguna sebesar 0,1230 total defect yang masuk dalam kategori kecil, dan tingkat kepuasan pengguna menggunakan kuesioner SUS memperoleh rerata SUS sebesar 54,45945946 yang artinya tingkat kepuasan pengguna rendah terhadap aplikasi berdasarkan penentuan nilai SUS yaitu Acceptability atau tingkat penerimaan penggunaan masuk dalam kategori marginal low, Grade Scale atau tingkat grade skala masuk dalam kategori F dan Adjective Rating masuk dalam kategori OK. Selain itu, pada SUS skor percentile rank masuk kedalam grade D.