Pouteria ramiflora leaf extract on emulgel in wound healing activity in diabetic rats

The genus Pouteria has been studied because it presents various activities, among which is its anti-inflammatory potential. The effects of Pouteria ramiflora Carbopol gel on the healing of skin wounds in diabetic rats were evaluated by microscopic imaging. Streptozotocin was administered intraperitoneally in animals that had fasted for 12 hours, a situation confirmed by the glycemic index (˃ 240 mg dL-1). An excision on the back of the animals was performed and three groups were formed: Control (Gel), Ethanolic extract (Ext) and Gel + extract 2% (Ext+gel); the histopathological evaluation occurred on the 7th, 14th, 21st and 30th days after the post-operative period. The results of the phytochemical prospecting of P. ramiflora extract demonstrated the major presence of phenolic compounds and flavonoids; the assessment of the inflammatory infiltrate on the 7th day was higher on group Ext and Ext+gel when compared to group Control; on the 14th day control and Ext (p<0.05). The quantification of fibroblasts was higher on the 7th day among the three treatments, control and Ext (p<0.05), on the 21st day. Angiogenesis showed a higher number of vessels in Ext+gel group (p<0.05) on the 7th day; in Control, Ext and Ext+gel (p<0.05) on the 14th day; and Control and Ext (p<0.05)on the 21st day. The histopathological results showed that the formulation Ext+gel was efficient in tissue reparation and decrease in inflammatory cells on the diabetic’s animals.

The use of chitosan as a skin-regeneration agent in burns injuries: A review

Chitosan is an amino-polysaccharide, traditionally obtained by the partial deacetylation of chitin from exoskeletons of crustaceans. Properties such as biocompatibility, hemostasis, and the ability to absorb physiological fluids are attributed to this biopolymer. Chitosan’s biological properties are regulated by its origin, polymerization degree, and molecular weight. In addition, it possesses antibacterial and antifungal activities. It also has been used to prepare films, hydrogels, coatings, nanofibers, and absorbent sponges, all utilized for the healing of skin wounds. In in vivo studies with second-degree burns, healing has been achieved in at least 80% of the cases between the ninth and twelfth day of treatment with chitosan coatings. The crucial steps in the treatment of severe burns are the early excision of damaged tissue and adequate coverage to minimize the risk of infection. So far, partial-thickness autografting is considered the gold standard for the treatment of full-thickness burns. However, the limitations of donor sites have led to the development of skin substitutes. Therefore, the need for an appropriate dermal equivalent that functions as a regeneration template for the growth and deposition of new skin tissue has been recognized. This review describes the properties of chitosan that validate its potential in the treatment of skin burns.

Combined Negative Effects of Microplastics and Plasticizer DEHP: the Increased Release of Nets Delays Wound Healing

Introduction: Environmental pollutants microplastics (MPs) and di (2-ethyl) hexyl phthalate (DEHP) can cause damage to multiple organs by causing oxidative stress. Oxidative stress participates in the healing of skin wounds through the release of neutrophil extranets (Nets). Here, we studied the effects of DEHP and MPs on skin wound healing in mice after single and combined exposure for 1 month. Results: The results showed that MPs delayed the healing of skin wounds, and the combination of the two delayed wound healing more significantly. The results of in vivo and in vitro experiments showed that the release of oxidative stress and Nets in the single exposure group increased, and the combined exposure group increased more. Further mechanism studies showed that the skin chemokines of the single exposure group increased, the NF-κB pathway was activated, the Wnt pathway was inhibited, and the epidermal growth factor and fibrosis-related indicators decreased. The combined exposure group showed a more obvious trend.Conclusion: In summary, the above results indicate that DEHP combined with MPs induces an increase in the release of Nets by causing excessive skin ROS production and increases the expression of chemokines and interferes with the expression of healing factors by regulating the NF-κB and Wnt pathways.

Green coffee oil cream (Coffea arabica L.): healing potential of skin wounds of wistar rats

Objective: To evaluate the effect of 10% cream of green coffee oil on skin Wounds in Wistar Rats. Materials and Methods: We used 36 Wistar Rats, randomly divided between 3 groups: Inert Group (I, n = 12), Wounds were cleaned and treated with cream without the active ingredient once a day; Green Coffee (CV, n = 12), Wounds were cleaned and treated with 10% green coffee cream once a day; Dexpanthenol (SD, n = 12), Wounds were cleaned and treated with 5% Dexpanthenol once a day. As lesions had been generated by 8mm drilling, the same have been treated topically every day. On the 3rd , 7th and 14th day after Surgery, four Animals / group were sacrificed, or a Fragment of skin around the Wound was collected and processed histologically. Results: Green coffee cream is a 10% stimulus in the Repair process, such as Wounds without signs of infection, and an increase in the number of mononuclear Cells and fibroblasts (p 0.05), good entanglement of collagen Fibers and less healing time presented to the other studied groups. Conclusion: 10% green coffee oil has Pharmacological potential in the healing of skin Wounds.

Karyometric Parameters of Skin Fibroblasts in the Early Stages of Wound Healing

The aim of the study was a comparative study of the karyometric parameters of fibroblasts using various acid-modified solutions in the early stages of healing of skin wounds in rats.Material and methods. The experiment was carried out on 75 Wistar rats. For the treatment of wounds, 0.9% NaCl solution was used at a pH of 5.0; 7.0; and 7.5. The material was fixed in Shtiva liquid, 10% formalin solution. Thin paraffin sections (4–5 μm) were stained with hematoxylin and eosin, azan according to Heidenhain. The area of fibroblast nuclei was determined planimetricallyResults. Three types of fibroblasts were found in the skin of intact rats. The papillary layer contained papillary fibroblasts; the reticular layer – reticular fibroblasts. The third type of fibroblasts was located between the hair follicles. In rats of all experimental groups, on the 1st day after the wound was inflicted, extensive inflammatory processes, hemorrhages, and necrotic changes in the structural components of the skin were revealed. On the 3rd day after the wound was inflicted in rats of the 1st and 4th groups, the area of the nuclei of papillary fibroblasts in the peripheral areas of the wound statistically significantly increased by 24.4% and 22.4%, respectively, compared with the control. In animals of the 3rd group, the area of fibroblast nuclei remained unchanged. On the 5th day, an increase in the number of fibroblasts over the entire surface of the wound was observed in rats of all experimental groups. The area of fibroblast nuclei in rats of the 4th group increased statistically significantly by 26.5% (Р<0.001) as compared to the control. In animals of the 3rd group, the increase in the area of the nuclei in comparison with the control was only 6.5%, while no statistically significant changes were found in comparison with the control. The area of the fibroblast nuclei located between the hair follicles reached a maximum value, which indicated a high functional tension of the hair follicle fibroblasts. Conclusion. The use of 0.9% NaCl solution with pH 5.0 in the first phase of wound healing and 0.9% NaCl solution with pH 7.5 in the second phase of wound healing reduces inflammation processes, while the secretory activity of fibroblasts increases, and collagenization is accelerated.

In vivo observation of lidocaine-encapsulated Polymyxin/glycol nanoparticles wound dressing for the treatment and care of abdominal pain incision in intensive care unit

The developments of lidocaine chloride loaded nanoparticles are encouraging biomaterials, which could be used for wound healing applications for abdominal pain management. The current work presents the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded Polymyxin (PMN)/Glycol (GLY). The LCH nanocomposite (LCH@PMN/GLY) were fabricated by the LCH oxide solutions within the PMN/GLY matrix. The influences of different experimental limitations on PMN/GLY nanoparticles formations were examined. The PMN/GLY and LCH@PMN/GLY nanoparticle sizes were evaluated by high resolution-scanning electron microscopy (HR-SEM). Additionally, the antibacterial efficacy of PMN/GLY and LCH@PMN/GLY was developed for gram-positive and negative microorganisms. Moreover, we examined in vivo healing of skin wounds formed in mouse models over 20 days. In contrast to the untreated wounds, rapid healing was perceived in the LCH@PMN/GLY-treated wound with less damage. These findings indicate that LCH@PMN/GLY-based bandaging materials could be a potential innovative biomaterial for tissue repair and implantation and nursing care for wound healing applications for abdominal pain incision in intensive care unit (ICU) management in an animal model.

Manfaat Olesan Madu Pada Penyembuhan Luka Kulit

The use of honey in wound care has been used since ancient times. Honey has been shown to have antibacterial properties, as well as low pH levels making environmental conditions unfavorable for bacterial growth. Clinical observations from human trials report that honey helps granulation tissue formation, increases epithelialization, and reduces inflammation which affects the acceleration of wound healing. The aims of this study is to determine the effect of honey toward wound healing. This study is in the form of a literature review. Literature is taken from one database, namely PubMed. The keywords used are honey and wound healing. After being selected by inclusion and exclusion criteria, ten literature will be reviewed. Honey gave good results and affected the healing of skin wounds, including several types of honey that were studied in experimental animals and in experimental people. In conclusion, honey has an effect on healing skin wounds.Key words: Honey, wound healing Abstrak: Penggunaan madu dalam perawatan luka telah digunakan sejak saat zaman kuno. Madu telah terbukti memiliki sifat antibakteri, juga kadar pH rendah membuat kondisi lingkungan yang tidak mendukung untuk pertumbuhan bakteri. Pengamatan klinis dari uji coba pada manusia melaporkan bahwa madu membantu pembentukan jaringan granulasi, meningkatkan epitelisasi, dan mengurangi peradangan yang mempengaruhi percepatan penyembuhan luka. Tujuan penelitian ini untuk mengetahui pengaruh madu terhadap penyembuhan luka. Penelitian ini dalam bentuk literature review. Literatur diambil dari satu database yaitu PubMed. Kata kunci yang digunakan yaitu honey and wound healing. Setelah diseleksi dengan kriteria inklusi dan eksklusi didapatkan sepuluh literatur yang akan direview. Madu memberikan hasil yang baik dan berpengaruh terhadap penyembuhan luka kulit, di antaranya ada beberapa jenis madu yang diteliti pada hewan percobaan maupun pada orang coba. Sebagai simpulan, madu mempunyai pengaruh terhadap penyembuhan luka kulit.Kata kunci: Madu, penyembuhan luka

Precise engineering of silver loaded polyvinyl alcohol nanogels for wound nursing care systems in operation room

This study was designed to establish the composition of wound bandages based on silver nanoparticle (AgNP)loaded polyvinyl alcohol (PVA) nanogels. The AgNP nanogel (Ag-nGel) was fabricated by the fructose-mediated reduction of silver nitrate solutions within the PVA matrix. The influence of different experimental limitations on PVA nanogel formations were examined. The nanogel particle sizes were evaluated by transmission electron microscopy and determined to range from ∼10–50 nm. Additionally, glycerol were added to the Ag-nGels, and the resulting compositions (Ag-nGel-Glu) were coated on cotton fabrics to generate the wound bandaging composite. The cumulative drug release profile of the silver from the bandage was found to be ∼38% of the total loading after two days. Additionally, antibacterial efficacy was developed for gram positive and negative microorganisms. Moreover, we examined in vivo healing of skin wounds formed in mouse models over 21 days. In contrast to the untreated wounds, rapid healing was perceived in the Ag-nGel-Glu-treated wound with less damaging. These findings indicate that Ag-nGel-Glu-based bandaging materials could be a potential candidate for wound bandaging applications in the future.