The effect of drone transport on the stability of biochemical, coagulation and hematological parameters in healthy individuals

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Desirée Perlee ◽  
Klaas Henk van der Steege ◽  
Gijs den Besten
Keyword(s):  
Hematological Parameters ◽  
Blood Parameters ◽  
Control Group ◽  
Healthy Individuals ◽  
Glucose Phosphate ◽  
Relevant Effect ◽  
Novel Approach ◽  
Variation Database ◽  
Allowable Error ◽  
The Stability

Abstract Objectives Transport of blood tubes is mainly by car or pneumatic transport. The transportation of blood tubes by drones is a novel approach for rapid transportation of blood tubes over long distances. However, limited data on the stability of biochemical, coagulation and hematological parameters is available after transport of blood tubes by drone. Methods To investigate the effect of drone transport on the stability of blood parameters, four test flights were performed. Blood was drawn from 20 healthy individuals and 39 of the most frequently measured blood parameters were compared between 4 groups; immediate measurement (control), late measurement, transport by car and transport by drone. Total Allowable Error (TAE) of the EFLM Biological Variation Database was used to determine the clinical relevance of significant differences. Results The majority of blood parameters were not affected by drone transport. Eight of the measured parameters showed significant differences between all the groups; glucose, phosphate, potassium, chloride, hemoglobin, platelet count, APTT and Lactate dehydrogenase (LD). A clinically relevant increase for LD after transport and a decrease for glucose values in time and after transport compared with the control group was shown. Conclusions Transportation of blood tubes from healthy individuals by drones has a limited clinically relevant effect. From the 39 investigated blood parameters only LD and glucose showed a clinically relevant effect.

scholarly journals RESVERATROL INCLUSION COMPLEX WITH β-CYCLODEXTRIN (RCD): CHARACTERIZATION AND EVALUATION OF TOXICITY IN WISTAR RATS

2016 ◽  
Vol 9 (1) ◽  
pp. 27 ◽  
Author(s):  
V. S. K. Nishihira ◽  
N. J. Mezzomo ◽  
M. D. Baldissera ◽  
R. A. Vaucher ◽  
C. G. Pinto ◽  
...  
Keyword(s):  
Inclusion Complex ◽  
Oral Administration ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Metabolic Diseases ◽  
Hematological Parameters ◽  
Density Lipoprotein ◽  
Blood Parameters ◽  
Control Group ◽  
Scanning Calorimetry

<p class="RSCB01ARTAbstract"><strong>Objective</strong>:<strong> </strong>The aim of this study was to characterise the resveratrol inclusion complex with β-cyclodextrin (RCD) and evaluate their toxicity in wistar rats.</p><p class="RSCB01ARTAbstract"><strong>Methods: </strong>The RCD were prepared in ultra-turrax. For characterization of the RCD were used: Fourier transform infra-red Spectroscopy, Nuclear Magnetic Resonance (NMR), Differential Scanning Calorimetry (DSC) and X-ray powder diffraction. The RCD and others 4 treatments were performed by the chronic oral administration in 35 rats during 60 ds. After the treatments they were euthanized and the serum blood were collected to analyzed some hemogram and biochemical parameters including aspartyl aminotransferase (AST); alanine aminotransferase (AST); phosphatase alkaline (ALP); total bilirubin (TB); direct bilirubin (DB); total protein (TP); total cholesterol (TC), triacylglycerol (TAG), very low-density lipoprotein (VLDL), high-density lipoprotein (HDL), calcium, iron and phosphate using fully automated biochemistry analyzer.</p><p class="RSCB01ARTAbstract"><strong>Results: </strong>The characterization results indicated a successful formation of the RCD. All hematological parameters analysed were within the normal values in all the groups. Furthermore, the hemogram and biochemical parameters were significantly (P&gt;0.05) similar to the control group.</p><p class="RSCB01ARTAbstract"><strong>Conclusion: </strong>The daily oral administration during 60 d of RCD are not harmful on blood parameters of Wistar rats. Thus, RCD can be used safely for treatment of some metabolic diseases.</p>

scholarly journals Hematological parameters of carp juveniles in terms of introducing Quercus cortex extract into diet

2019 ◽  
Vol 2019 (4) ◽  
pp. 124-131
Author(s):  
Elena Petrovna Miroshnikova ◽  
Julia Vladimirovna Kilyakova ◽  
Sergey Vladimirovich Ponomarev ◽  
Azamat Ersainovich Arinzhanov ◽  
Maria Sergeevna Miroshnikova
Keyword(s):  
Hematological Parameters ◽  
Blood Parameters ◽  
Average Concentration ◽  
Control Group ◽  
Physiological Status ◽  
Metabolic Processes ◽  
Maximum Increase ◽  
Dehydrogenase Enzyme ◽  
Feed Analysis ◽  
Experimental Group

The article presents the results of studies of morphological and biochemical blood parameters of carp juveniles when adding the extract Quercus cortex (1, 2, 3 mg/kg of feed), probiotic preparation of Soya-bifidum (0.7 ml/kg of feed), antibiotics Ciprofloxacin hydrochloride in the composition of the drug Antibak 250 (100 mg/kg of feed). Analysis of hematological parameters of carp juveniles showed that adding Quercus cortex extract to the diet at a dosage of 2 mg/kg of feed contributed to increasing SGE by 66%, the average concentration of hemoglobin in the red blood cell by 20% compared to the control group. An increase in the level of leukocytes compared to the control was recorded only in the IV experimental group and made 13% (P≤0.05). An increase in glucose in the groups receiving Quercus cortex extract has been stated: in the I experimental group- by 4.2 times (P≤0.001), in II – by 3.4 (P≤0.001) and in III – by 3.3 (P≤0.001) compared to the control. Increasing glucose concentration indicates active metabolic processes and better stress resistance in the fish body. Iron content in carp blood was higher only in the first experimental group (by 3 times) compared to the control. Increasing activity of alanine aminotransferase was observed in all experimental groups, the highest rate of this enzyme being recorded in the IV experimental group (feed with probiotic additive Soya-bifidum) with a significant excess of control by 48%. Lactate dehydrogenase enzyme activity exceeded the control in all experimental groups, maximum increase (by 67%) was observed in the IV experimental group. Alkaline phosphatase was below the control in all experimental groups, minimum values being registered in the IV experimental group- by 37% less than the control. Indicators of p-amylase in the IV group exceeded the control by 2.6 times, in the V group – by 1.5 times. In the II group p-amylase was lower than the control by 44%, and in the III – by 1.9 times. According to the obtained hematological data, it has been established that including Quercus cortex extract into the diet of carp juveniles is promising due to the positive effect on the fish physiological status, immune status, metabolic processes, hematopoiesis and digestibility of feed.

scholarly journals EVALUATING SOME OF THE HEMATOLOGICAL PARAMETERS FOR HELICOBACTER PYLORI INFECTED PATIENTS

2020 ◽  
Vol 17 (36) ◽  
pp. 1052-1060
Author(s):  
Nidhal Abdullah HASHIM ◽  
Younus Jasim ABDULLAH ◽  
Hasan Rahman SHAWI
Keyword(s):  
Helicobacter Pylori ◽  
Complete Blood Count ◽  
Hematological Parameters ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Healthy Individuals ◽  
Corpuscular Hemoglobin ◽  
Distribution Width ◽  
Significant Difference ◽  
H Pylori

Helicobacter pylori are Gram-negative curved rods that habitats the gastric mucosa and considered as the leading causes of stomach ulcers and duodenal ulcers in humans. The main object of this study was to evaluate the influences of the bacteria on several hematological parameters. A total of 60 patients aged between 15 to 40 years were included in this study (30 male and 30 female) in addition to 30 healthy individuals from the same ages who were considered as a control group. Venous blood (4 ml) was obtained from the study population and investigated for complete blood count (CBC) using an automated hematology analyzer. The results revealed that there is a substantial decrease (p 0.01) in Hemoglobin (Hb), Red cell distribution width (RDW), and mean corpuscular hemoglobin (MCH) in patients compared to control. However, no significant difference in Red blood cells (RBCs), Hematocrit (HCT), mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC) in patients compared to control. Also, no significant differences were found in the white blood cell (WBC) between H. pylori infected and non-infected persons. Otherwise, there are significant differences (p 0.01) in lymphocytes, monocytes, granulocytes in the patients and healthy individuals. The results also found that there are significant statistical differences in some of the hematologic parameters among study groups according to their gender. The study concluded that Hb, RDW, lymphocytes, monocytes, granulocytes are decreased in H. Pylori infected patients suggesting that infection may have direct effects on blood parameters.

scholarly journals Protective effect of blackthorn fruits (Prunus spinosa) against tartrazine toxicity development in albino Wistar rats

2019 ◽  
Author(s):  
Igori Balta ◽  
Bogdan Sevastre ◽  
Vioara Mireșan ◽  
Marian Taulescu ◽  
Camelia Raducu ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Hematological Parameters ◽  
Blood Parameters ◽  
Control Group ◽  
Water Mixture ◽  
Water Group ◽  
Prunus Spinosa ◽  
Food Dye ◽  
Group I ◽  
Group Ii

Background: Tartrazine (Yellow 5 or E102) is a synthetic food dye able to modify perception and behavior, causingagitation, confusion, rhinitis and can produce hyperactivity syndrome in children when is combined with benzoates.Additionally, it can trigger oxidative stress which consequently generates metabolic disorders. Therefore, the studywas designed to evaluate the harmful effects of the food additive tartrazine and to observe beneficial properties ofblackthorn fruits (Prunus spinosa) on the blood and organs of albino Wistar rats.Materials and methods: This study was carried out on 20 mature Wistar rats, randomly divided into four groupsof five animals. Over the course of the experiment, the control group received only food and drinking water, group Ireceived 75 mg of tartrazine dissolved in (250 ml) water group II was given 75 mg of tartrazine and 200 mg of driedblackthorn fruit powder 200 mg dissolved simultaneously in (250 ml) of tartrazine-water mixture (aiming to reducethe tartrazine toxicity) and group III received a higher dose of tartrazine (100 mg) in (250 ml) of water.Results: At the end of the experiment, values regarding kidney and liver weight were significantly increased, whilethe weight of the spleen was slightly decreased compared with the weight of the control group. Biochemical andhematological assays, of the blood samples show that the addition of tartrazine in the diet of rats caused significantchanges in all biochemical and hematological parameters of the blood. In the group II, which received (P. spinosa)powder combined with tartrazine, the biochemical and hematological parameters had average values similar to thecontrol group.Conclusions: Histopathological assay showed that the application of tartrazine in the group I, II and III producedlesions of the kidneys, spleen and the liver for all rodents. Tartrazine was able to generate histopathological changes,which caused significantly tissue lesions of the liver and significant changes in blood parameters. Blackthorn powdershowed a promising protective role for the blood parameters but demonstrated no significant benefits for the organs.

scholarly journals Influence of Bacterial Organic Selenium on Blood Parameters, Immune Response, Selenium Retention and Intestinal Morphology of Broiler Chickens

2020 ◽  
Author(s):  
A. M. Dalia ◽  
T. C. Loh ◽  
A. Q. Sazili ◽  
Anjas Asmara Samsudin
Keyword(s):  
Broiler Chickens ◽  
Hematological Parameters ◽  
Basal Diet ◽  
Blood Parameters ◽  
Intestinal Morphology ◽  
Control Group ◽  
Villus Height ◽  
Organic Selenium ◽  
Significant Difference ◽  
Immunity Response

Abstract Background: Several studies indicated that dietary organic selenium (Se) usually absorbed better than an inorganic source, with high retention and bioavailability. Dietary Se as an antioxidant element affects the immune system and hematological status in animals. Therefore, the aim of this study was to evaluate the effect of dietary supplementation of bacterial selenium as an organic source on hematology, immunity response, selenium retention, and gut morphology in broiler chickens. Results: The present results revealed that supplementation of inorganic Se was associated with the lowest level of RBC, HB, and PCV with significant difference than ADS18-Se. In the starter stage, both T2 and T5 were associated with the significantly highest IgG level compared to the basal diet, while all supplemented groups showed higher IgM levels compared to the control group. In the finisher phase, all Se supplemented groups showed significant (P ˂ 0.05) increases in IgG, IgA, and IgM levels compared to T1. Birds fed bacterial-Se showed high intestinal villus height and better Se retention more than sodium selenite. The organic selenium of ADS18 had a superior action in improving Se retention compared to ADS1 and ADS2 bacterial Se. Conclusion: Bacterial organic Se had a beneficial effect on the villus height of small intestine led to high Se absorption and retention. Thus, it caused a better effect of Se on hematological parameters and immunity response.

scholarly journals Effects of colistin sulfate on hematological parameters in broiler

2021 ◽  
Vol 7 (2) ◽  
pp. 113-117
Author(s):  
Md Nazmul Hasan ◽  
Md Shafiqul Islam ◽  
Md Rakibul Hasan ◽  
Kazi Rafiqul Islam
Keyword(s):  
Hematological Parameters ◽  
Blood Parameters ◽  
Control Group ◽  
Broiler Chicks ◽  
Withdrawal Period ◽  
Colistin Sulfate ◽  
Significant Difference ◽  
Antibiotic Group ◽  
Group A ◽  
Group B

Hematological indication is an important finding for pathophysiological analysis of biological science. This study was designed with the aim to investigate the effects of residual antibiotics on hematological parameters of broiler following discriminate and indiscriminate use. The day old broiler chicks were collected and reared up to 31 days. The treatment was started from the day 16th until sacrifice. The chicks were randomly divided into three groups namely control group (Group A), discriminate antibiotic group (Group B) and indiscriminate antibiotic group (Group C) on the 14th day. The discriminate group was treated with antibiotic, colistin sulfate maintaining the withdrawal period of one week. In case of indiscriminate group the withdrawal period was not maintained and antibiotic treatment was continued till the day of sacrifice. The Hb (%) of control, discriminate and indiscriminate groups were 8.34±0.15, 6.89±0.27, and 7.14±0.23 respectively. The differences among means were statistically significant (P<0.05). The Packed Cell Volume (PCV) of control, discriminate, and indiscriminate groups were 23.51±0.76, 21.17±0.94, and 22.83±1.19 respectively. The differences among means were statistically significant. The multiple pair wise comparison of means of blood parameters revealed that there was no significant difference among discriminate and indiscriminate groups. From the above findings, this research could be considered a need based research in Bangladesh to ascertain the influential effect of antibiotic on hematological parameters in broiler. Asian J. Med. Biol. Res. 2021, 7 (2), 113-117

scholarly journals Influence of Bacterial Organic Selenium on Blood Parameters, Immune Response, Selenium Retention and Intestinal Morphology of Broiler Chickens

2020 ◽  
Author(s):  
A. M. Dalia ◽  
T. C. Loh ◽  
A. Q. Sazili ◽  
Anjas Asmara Samsudin
Keyword(s):  
Broiler Chickens ◽  
Hematological Parameters ◽  
Basal Diet ◽  
Blood Parameters ◽  
Intestinal Morphology ◽  
Control Group ◽  
Villus Height ◽  
Organic Selenium ◽  
Significant Difference ◽  
Immunity Response

Abstract Background: Several studies indicated that dietary organic selenium (Se) usually absorbed better than an inorganic source, with high retention and bioavailability. Dietary Se as an antioxidant element affects the immune system and hematological status in animals. Therefore, the aim of this study was to evaluate the effect of dietary supplementation of bacterial selenium as an organic source on hematology, immunity response, selenium retention, and gut morphology in broiler chickens. Results: The present results revealed that supplementation of inorganic Se was associated with the lowest level of RBC, HB, and PCV with significant difference than ADS18-Se. In the starter stage, both T2 and T5 were associated with the significantly highest IgG level compared to the basal diet, while all supplemented groups showed higher IgM levels compared to the control group. In the finisher phase, all Se supplemented groups showed significant (P ˂ 0.05) increases in IgG, IgA, and IgM levels compared to T1. Birds fed bacterial-Se showed high intestinal villus height and better Se retention more than sodium selenite. The organic selenium of ADS18 had a superior action in improving Se retention compared to ADS1 and ADS2 bacterial Se. Conclusion: Bacterial organic Se had a beneficial effect on the villus height of small intestine led to high Se absorption and retention. Thus, it caused a better effect of Se on hematological parameters and immunity response.

scholarly journals Influence of bacterial organic selenium on blood parameters, immune response, selenium retention and intestinal morphology of broiler chickens

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
A. M. Dalia ◽  
T. C. Loh ◽  
A. Q. Sazili ◽  
A. A. Samsudin
Keyword(s):  
Broiler Chickens ◽  
Hematological Parameters ◽  
Basal Diet ◽  
Blood Parameters ◽  
Intestinal Morphology ◽  
Control Group ◽  
Villus Height ◽  
Organic Selenium ◽  
Significant Difference ◽  
Immunity Response

Abstract Background Several studies indicated that dietary organic selenium (Se) usually absorbed better than an inorganic source, with high retention and bioavailability. Dietary Se as an antioxidant element affects the immune system and hematological status in animals. Therefore, the aim of this study was to evaluate the effect of dietary supplementation of bacterial selenium as an organic source on hematology, immunity response, selenium retention, and gut morphology in broiler chickens. Results The present results revealed that supplementation of inorganic Se was associated with the lowest level of RBC, HB, and PCV with significant difference than ADS18-Se. In the starter stage, both T2 and T5 were associated with the significantly highest IgG level compared to the basal diet, while all supplemented groups showed higher IgM levels compared to the control group. In the finisher phase, all Se supplemented groups showed significant (P ˂ 0.05) increases in IgG, IgA, and IgM levels compared to T1. Birds fed bacterial-Se showed high intestinal villus height and better Se retention more than sodium selenite. The organic selenium of ADS18 had a superior action in improving Se retention compared to ADS1 and ADS2 bacterial Se. Conclusions Bacterial organic Se had a beneficial effect on the villus height of small intestine led to high Se absorption and retention. Thus, it caused a better effect of Se on hematological parameters and immunity response.

scholarly journals Influence of Bacterial Organic Selenium on Blood Parameters, Immune Response, Selenium Retention and Intestinal Morphology of Broiler Chickens

2020 ◽  
Author(s):  
A. M. Dalia ◽  
T. C. Loh ◽  
A. Q. Sazili ◽  
Anjas Asmara Samsudin
Keyword(s):  
Broiler Chickens ◽  
Hematological Parameters ◽  
Basal Diet ◽  
Blood Parameters ◽  
Intestinal Morphology ◽  
Control Group ◽  
Villus Height ◽  
Organic Selenium ◽  
Significant Difference ◽  
Immunity Response

Abstract Background Several studies indicated that dietary organic Se usually absorbed better than inorganic Se with high retention and bioavailability. Dietary Se as an antioxidant element affects the immune system and hematological status in animals. Therefore, the aim of this study was to evaluate the effect of dietary supplementation of bacterial selenium as organic source on hematology, immunity response, selenium retention and gut morphology in broiler chickens.Results The present results revealed that supplementation of inorganic Se was associated with the lowest level of RBC, HB, and PCV with significant difference than ADS18-Se. In the starter stage, both T2 and T5 were associated with the significantly highest IgG level compared to the basal diet, while all supplemented groups showed higher IgM level compared to control group. In the finisher phase, all Se supplemented groups showed significant (P ˂ 0.05) increases in IgG, IgA and IgM levels compared to T1. Birds fed bacterial-Se showed high intestinal villus height and better Se retention more than sodium selenite. Selenium of ADS18 had a superior action in improving Se retention compared to ADS1 and ADS2 bacterial Se.Conclusion Bacterial organic Se had a beneficial effect on villus height of small intestine led to high Se absorption and retention. Thus, caused better effect of Se on hematological parameters and immunity response.

scholarly journals Statistical characteristics of the components of coagulation hemostasis and the degree of oxygenation of rat blood in the normal and at different times of the experimental opioid effect

Morphologia ◽  
2021 ◽  
Vol 15 (3) ◽  
pp. 125-136
Author(s):  
Ye.V. Paltov ◽  
Z.Z. Masna ◽  
V.B. Fik ◽  
I.V. Chelpanova ◽  
N.O. Ambarova
Keyword(s):  
Prothrombin Time ◽  
Hematological Parameters ◽  
Blood Platelet ◽  
Blood Parameters ◽  
Male Rats ◽  
Statistical Characteristics ◽  
Control Group ◽  
Experimental Animals ◽  
Prothrombin Index

Background. The problem of non-drug use of opioid drugs occupies a significant place among the current problems of world medicine. Objective. Тo study the hematological parameters of coagulation hemostasis in the norm and the dynamics of their changes at different times of opioid exposure. Methods. The experimental study was performed on sexually mature, outbred male rats in the number of 80 animals, weighing 160-270 g, aged 4.5-7.5 months. Animals were injected intramuscularly with “Nalbuphine” once daily for one day (10-11 hours in the morning) for 98 days. The initial dose of nalbuphine during the first 2 weeks was 0.212 mg / kg, the next 2 (II - IV weeks) - 0.225 mg / kg, the next (IV - VI weeks) - 0.252 mg / kg, the next (VI - VIII weeks) ) - 0.260 mg / kg, the next (VIII - X weeks) - 0.283 mg / kg, the next (X - XII weeks) - 0.3 mg / kg, and during (XII - XIV weeks) - 0.454 mg / kg. Thus, the conditions for chronic opioid exposure were created. Animals are divided into 3 groups. The 1-st group of animals received Nalbuphine for 98 days, with subsequent collection of material (end of 2 weeks, 4 weeks, 6 weeks, 8 weeks, 10 weeks, 12 weeks and 14 weeks of experimental opioid exposure); The 2-d was the control group, which for 98 days received injections of saline intramuscularly in one period of time (10 - 11 o'clock in the morning). Blood sampling and study of hematological parameters of blood (platelet count, prothrombin time, prothrombin index, time of recalcification of stabilized blood, total fibrinogen, determination of hemoglobin, hematocrit) were performed according to conventional methods. Software R v 4.0.3 and RStudio v 1.2.5042 were used for statistical calculations and graphing. MSOffice Excel 2010 spreadsheets were used to generate the final tables and store the data. Results. The key to the dynamics of changes in the blood parameters of experimental animals was week 6 of the experiment, as most indicators had the highest dynamics up to 6 weeks including further indicators of stability, which was higher (fibrinogen and prothrombin index) or less (prothrombin time, recalcification time and hemoglobin) indicators of the control group. The blood hematocrit of the experimental animals decreased evenly at all study terms to a minimum value at 14 week, and the number of platelets evenly all times increased to a maximum value at the last term of the experiment. This trend in all indicators was confirmed statistically. Conclusion. Our research has made it possible to study first and then observe the dynamics of changes in coagulation hemostasis and the degree of oxygenation of blood in acute, subchronic and chronic periods of experimental opioid exposure with subsequent statistical comparison.

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