Comparative study of two bioassays with weakened duckweed and yeast treated with homeopathic preparations

In homeopathic basic research, the question as to the most adequate test systems and apt methodology is still open. This investigation examined the hypothesis that more complex organisms show stronger reactions to homeopathic remedies than less complex ones. We compared two Arsenic (As5+) stressed bioassays with duckweed (Lemna gibba, a multi-cellular autotrophic organism) and yeast (Saccharomyces cerevisiae, a single-cellular heterotrophic organism) regarding their response to homeopathic preparations [1]. For duckweed, growth rates of leaf area and leaf number were evaluated. For yeast, growth kinetics were determined by measuring slope, yield and Et50 (point in time when yield was half maximum) of the sigmoid growth curve. The experiments with duckweed and yeast were performed in parallel (same day, same location and identical homeopathic preparations). After screening 17 substances, three homeopathic preparations (Arsenicum album, nosode, gibberellic acid) were chosen for repeated experimental series [2]. Five independent experiments were conducted for each remedy with both organisms in parallel. Potency levels used were in the range of 17x–33x for duckweed and 17x–30x for yeast. To control for test system stability, systematic negative control experiments were conducted over the complete experimentation period. All experiments were blinded and randomized. The systematic negative control experiments did not yield any significant effects. Application of potentized Arsenicum album in the duckweed bioassay yielded the largest effects compared to water controls without remedies for the parameters leaf area and leaf number (p

The ICECAP-A instrument for capabilities: assessment of construct validity and test–retest reliability in a general Dutch population

Purpose The ICEpop CAPability measure for Adults (ICECAP-A) assesses five capabilities that are important to one’s well-being. The instrument might be an important addition to generic health questionnaires when evaluating quality of life extending beyond health. This study aimed to conduct a psychometric assessment of the Dutch translation of the ICECAP-A. Methods Construct validity of the instrument was assessed in two ways. First, by measuring correlations with the EQ-5D-5L questionnaire and a measure of self-efficacy and, second, by investigating the ability to distinguish between groups known to differ on the construct the ICECAP-A means to capture. Additionally, test–retest reliability was evaluated. Results In total, 1002 participants representative of the general Dutch population completed an online survey. For test–retest reliability, 252 participants completed the same questionnaire 2 weeks later. The ICECAP-A indicated moderate to strong correlations with the EQ-5D-5L and a strong correlation with self-efficacy. Furthermore, it was capable of differentiating known groups. Moreover, results indicated adequate test–retest reliability with an intraclass correlation coefficient of 0.79. Conclusion In summary, results suggest adequate test–retest reliability and construct validity and indicate that the ICECAP-A might be of added value, especially when considering areas outside of the traditional health intervention model.

Psychometric Properties of the Infant Toddler Activity Card Sort

The Infant Toddler Activity Card Sort (ITACS) is a new measure that engages caregivers in goal development by measuring participation in early childhood. Initial evaluation of the ITACS is required to assess its psychometric properties. To evaluate test–retest reliability, concurrent validity, and between-group differences of the ITACS. Caregivers ( N = 208) of children 0–3 years, who were typically developing (TD) or had a developmental delay (DD), completed the ITACS and Pediatric Evaluation of Disability Inventory–Computer Adaptive Test (PEDI-CAT) with repeat assessment after 2 weeks later ( n = 117). The ITACS demonstrated moderate concurrent validity with the PEDI-CAT. Children with DD had significantly more activity concerns on the ITACS than TD. Adequate test–retest reliability was not achieved. The ITACS is a useful tool for identifying caregiver concerns in activity engagement; interventions can be tailored to address concerns and increase participation in childhood routines.

Special Issue on Digital Pathology, Tissue Image Analysis, Artificial Intelligence, and Machine Learning: Approximation of the Effect of Novel Technologies on Toxicologic Pathology

For decades, it has been postulated that digital pathology is the future. By now it is safe to say that we are living that future. Digital pathology has expanded into all aspects of pathology, including human diagnostic pathology, veterinary diagnostics, research, drug development, regulatory toxicologic pathology primary reads, and peer review. Digital tissue image analysis has enabled users to extract quantitative and complex data from digitized whole-slide images. The following editorial provides an overview of the content of this special issue of Toxicologic Pathology to highlight the range of key topics that are included in this compilation. In addition, the editors provide a commentary on important current aspects to consider in this space, such as accessibility of publication content to the machine learning-novice pathologist, the importance of adequate test set selection, and allowing for data reproducibility.

Large differences in community COVID-19 testing across geographic areas in a Swedish region with 385,000 inhabitants

Background Sufficient community testing for suspected COVID-19 regardless of residential area is essential for a successful test-trace-isolate strategy. Aim This study aimed to elucidate area level characteristics linked to testing rates. Methods Free-of-charge diagnostic tests (PCR) of SARS-CoV-2 was made available to the general public in late June 2020 in Uppsala County, Sweden, at four main test stations, and to a lesser extent at other health care units. We analysed 35,794 tests performed on individuals from 346 postal codes, from 24 June to 12 October 2020. Results We observed varying testing rates across postal code areas within Uppsala City as well as in Uppsala County. Testing rates were lower in areas characterized by longer distance to the nearest test station, lower neighbourhood deprivation index indicating higher deprivation (NDI) and higher proportion of inhabitants with foreign background. Multivariable regression models could not separate influences of foreign background and NDI on COVID-19 testing rates as these were collinear. Further, we did not detect any association between COVID-19 hospitalization rates and testing rates, indicating that underlying community infection rates did not substantially affect test frequency during this period. Conclusion We observed that testing rates were associated with distance to test station and socioeconomic and demographic circumstances. As lower testing rates can contribute to inequity in pandemic health effects, there is an urgent need to ensure adequate test accessibility in all parts of society.

Modelling the potential impact of mask use in schools and society on COVID-19 control in the UK

Recent findings suggest that an adequate test-trace-isolate (TTI) strategy is needed to prevent a secondary COVID-19 wave with the reopening of society in the UK. Here we assess the potential importance of mandatory masks in the parts of community and in secondary schools. We show that, assuming current TTI levels, adoption of masks in secondary schools in addition to community settings can reduce the size of a second wave, but will not prevent it; more testing of symptomatic people, tracing and isolating of their contacts is also needed. To avoid a second wave, with masks mandatory in secondary schools and in certain community settings, under current tracing levels, 68% or 46% of those with symptomatic infection would need to be tested if masks’ effective coverage were 15% or 30% respectively, compared to 76% and 57% if masks are mandated in community settings but not secondary schools.

Self-Esteem in 60 Seconds: The Six-Item State Self-Esteem Scale (SSES-6)

With 20 items, the State Self-Esteem Scale (SSES) can be cumbersome in settings that demand efficiency. The present research created an efficient six-item version of the SSES that preserves score reliability and validity and its three-dimensional structure: social, appearance, and performance self-esteem. Item response theory and confirmatory factor analyses identified the “best” six items—two from each dimension (Study 1). Participants completed the SSES four times at 2-week intervals (Studies 2 and 3). The six-item SSES’ scores showed adequate test–retest reliability, explained substantial variance in trait-relevant measures, and showed convergent validity with related self-esteem measures. Participants completed the SSES and a laboratory experiment where they received negative feedback on an essay they had written and could retaliate against their evaluator by allocating hot sauce for them to consume (Study 4). The six-item SSES interacted with self-esteem instability in expected ways to predict hot sauce allocated.

The Students' Attitudes Towards Women'S Childbirth Experience - Development and Validation of a Questionnaire for Health Sciences Students (Cave-St)

BACKGROUND: The purpose of this study is to report on the validation process of the Spanish version of a questionnaire that explores health science students’ attitudes towards women’s childbirth experiences. This questionnaire could help inform education programs to enhance the quality of woman-professional interactions, and to improve women’s experiences of childbirth. METHODS: A standardised procedure for the development and validation of the questionnaire included: item development and psychometric pre-validation, Cronbach’s Alpha and split-half coefficients calculation, test–retest and item-total correlation for the reliability analysis. Content validity was undertaken by Delphi method with sixteen panelists over two rounds. We determined the factor structure and refined and validated the questionnaire according to the responses of a cohort of 160 students using principal components factor analysis with varimax rotation. RESULTS: A 52-items questionnaire CAVE-st: (acronym for cuestionario de actitudes sobre vivencias y experiencias en el parto) was developed and validated. The questionnaire had a high construct validity, with a Cronbach´s alpha > 0.92. The intraclass correlations (0.80) indicated adequate test–retest reliability. The results of the exploratory factor analysis revealed fourteen latent components that explained more than the 70% of the observed total variance.CONCLUSIONS: The Spanish version of the questionnaire CAVE-st has been developed and validated. Its psychometrics indicate that it is a valid and reliable tool to assess health sciences students’ attitude towards women´s childbirth experience. Further work to translate and adapt the instrument in other cultures and languages will be undertaken.

The Quest for an Adequate Test: Justifying the Sustainable City as an Order of Worth

The sustainable city represents an ideal of good and just living that has inspired urban development work for at least 25 years. While criticized by many for its scientific, social and political vagueness, the concept of the sustainable city has nonetheless continued to frame material and political efforts in urban redevelopment. From a perspective grounded in the pragmatic sociology of critique, this article takes this phenomenon as evidence of an international movement to generate not just political pronouncements or technical fixes, but a new order of worth, from the concept of the sustainable city. After presenting the pragmatic sociology of critique and the application of this body of social research as it pertains to better understanding sustainable urban development, we reflect on the factors that challenge the acceptance of the sustainable city as an order of worth, or as a mode and manner of justifying significant decisions in the public domain, recognizable and understandable to a majority. For efforts to create the sustainable city to justify themselves, socioculturally, in this way, the work demands a clear test of worthiness. This article illustrates the search for an adequate test through a review of two distinct efforts to generate new systems of assessment for sustainable building projects, and points out the contrasting nature of these two tests: one which aims to be accessible to thoroughgoing public debate fit to transform a context toward a political discourse of urban sustainability as well-being; the other that interprets the need for a test as affirmation of expertise related to the unfolding climate emergency.