RESEARCH OF THE PROCESS OF OBTAINING INTERESTERIFIED FAT FOR MARGARINE PRODUCTION ON THE BASIS OF SUNFLOWER OIL AND PALM STEARIN

The study presents the results of transesterification of a mixture of sunflower oil and palm stearin in various proportions and dilatometric analysis of the obtained transesterifications was carried out. For example, the ratios in the mixture of sunflower oil and palm stearin 15/85, 25/75, 30/70, 50/50 were subjected to transesterification in the laboratory, and the transesterifications used for the spread and margarine were obtained, and the optimal mixture ratios were determined. By dilatometric analysis, it was found that it is possible to obtain interesterificates in the ratio of a mixture of sunflower oil and palm stearin at 50/50 for a good spreading and high plasticity spread (t = 31 ℃), at 30/70 for margarine providing porosity for baking (t = 39 ℃) , at 25/75 to margarine used for puff pastry (t = 43 ℃). In addition, the fatty acid content of sunflower oil and palm stearin used in the experiments was also determined.

Evaluation of the role of interesterified fat in metabolic parameters and cellular adipose tissue stress pathways

The western lifestyle is characterized with increased intake of processed and ultraprocessed foods, which have trans fat in their composition. As an alternative to the of trans fat, interesterification is one of the process that have being used by the food industry for the production of plastic fats and trans free. Thus, the aim of this study was to evaluate the role of interesterified fat on metabolic parameters, lipid profile and cellular stress markers in white adipose tissue in mice.

Maternal intake of trans-unsaturated or interesterified fatty acids during pregnancy and lactation modifies mitochondrial bioenergetics in the liver of adult offspring in mice

The quality of dietary lipids in the maternal diet can programme the offspring to diseases in later life. We investigated whether the maternal intake of palm oil or interesterified fat, substitutes for trans-unsaturated fatty acids (FA), induces metabolic changes in the adult offspring. During pregnancy and lactation, C57BL/6 female mice received normolipidic diets containing partially hydrogenated vegetable fat rich in trans-unsaturated fatty acids (TG), palm oil (PG), interesterified fat (IG) or soyabean oil (CG). After weaning, male offspring from all groups received the control diet until day 110. Plasma glucose and TAG and liver FA profiles were ascertained. Liver mitochondrial function was accessed with high-resolution respirometry by measuring VO2, fluorimetry for detection of hydrogen peroxide (H2O2) production and mitochondrial Ca2+ uptake. The results showed that the IG offspring presented a 20 % increase in plasma glucose and both the IG and TG offspring presented a 2- and 1·9-fold increase in TAG, respectively, when compared with CG offspring. Liver MUFA and PUFA contents decreased in the TG and IG offspring when compared with CG offspring. Liver MUFA content also decreased in the PG offspring. These modifications in FA composition possibly affected liver mitochondrial function, as respiration was impaired in the TG offspring and H2O2 production was higher in the IG offspring. In addition, mitochondrial Ca2+ retention capacity was reduced by approximately 40 and 55 % in the TG and IG offspring, respectively. In conclusion, maternal consumption of trans-unsaturated and interesterified fat affected offspring health by compromising mitochondrial bioenergetics and lipid metabolism in the liver.