Antibody-Conjugated Magnetic Beads For Sperm Sexing By A Multi-Wall Carbon Nanotube Microfluidic Device

Bull production industries demand specific sexual bulls in response to different business productivity requirements. Therefore, sexual separation of sperm is essential for the livestock industry, increasing the demand for assisted reproductive technologies (ARTs). A popular sexual separation method in ART is monoclonal antibody enhancement, which does not affect the quality of the sperm and has specificity with high sexual separation accuracy. Moreover, microfluidic technique is an innovative tool for sperm separation, with increased installation efficiency and convenience but low accuracy and speed of detection. In this study, particles were separated using magnetic beads, magnetic beads on monoclonal antibodies, and sperm with the function of magnetic beads on monoclonal antibodies by controlling the microfluidic system fluid flow rate at 2 µL/min and releasing a 2.5-V electric field. This microfluidic device separated 100% and 95.42% of the magnetic beads and magnetic beads on monoclonal antibodies, respectively. The sperm separation test results showed that positively charged sperm Y moved to the negative electrical fluid channel with a specially designed multi-walled carbon nanotube microfluidic device that showed an X- and Y-sperm separation with a success rate of 80.12%. Compared with natural pregnancy (X- and Y-sperm ration 50:50, 50%), our proposed method is approximately 30.12% better.

Application of the Folgar–Tucker model to predict the orientation of particles of different aspect ratios in polymer suspensions

The increasing requirements on plastic parts demand a rising use of combined functional and reinforcing materials. Therefore, often reinforcing particles with different aspect ratios are added to the plastic as additive mixtures. However, the engineering design process of reinforced parts requires an early knowledge of the expected orientation of the reinforcing particles. Numerous models try to predict the orientation of particles in polymer suspensions. However, the interaction coefficient strongly depends on the aspect ratio of the particles and a prediction of the orientation behavior of additive mixtures with differently shaped particles has not been validated using conventional methods. In this work, the orientation of differently shaped particle mixtures in polymer suspensions is investigated for different fluid channel geometries. Finally, the Folgar–Tucker model is applied to filler mixtures and implemented into OpenFOAM®, which enables the comparison of filler orientation in different fluid channel geometries. Regarding the experiments a characteristic increase of the interaction coefficient was observed at a filling level of 5%. Furthermore, it was shown that a balanced mixing ratio yields higher interaction coefficients. With regard to the performed simulations, it was possible to show qualitatively how a considered interaction between fibers and platelets affects the orientations.

Sistem Kontrol Otomatis Misting Antiseptic Berbasis Mikrocontroller Untuk Meminimalisir Penyebaran Covid-19

Covid-19 merupakan penyebab infeksi saluran pernapasan. Penyebaran Covid-19 dapat diminimalisir dengan bebeberapa cara diantaranya penyemprotan antiseptik povidone iodine. Tujuan penelitian ini adalah menghasilkan alat yang berguna pada usaha meminimalisir penyebaran Covid-19 dengan sistem kontrol otomatis misting antiseptik berbasis microcontroller. Penelitian ini menggunakan metode penelitian pengembangan. Penelitian ini dilaksanakan di rumah peneliti dan dirampungkan di laboratorium terpadu Pendidikan Teknik Elektro Universitas Negeri Manado. Sistem kontrol otomatis misting antiseptik berbasis microcontroller merupakan sistem yang dibangun pada alat penyemrotan antiseptik povidone iodine. Sistem kontrol otomatis misting antiseptik berbasis microcontroller dapat digunakan untuk meminimalisir penyebaran Covid-19. Misting antiseptik berbasis microkontroller ini dapat bekerja sesuai dengan sistem yang dirancang untuk menyemprotkan antiseptik Povidone Iodine untuk tubuh manusia, cara kerjanya yaitu ketika ada benda yang berada pada titik atau jarak yang sudah ditentukan maka sensor gerak akan mendeteksinya dan akan mengirimkan sinyal ke microkontroller selanjutnya akan diproses ke aktuator/pompa yang akan mengalirkan cairan atau fluida tersebut ke nozzle misting 0.2 mm sebagai saluran fluida yang diposisikan untuk memberikan turbelensi ke fluida dalam hal ini cairan antiseptik povidone iodine untuk keluar melalui aparetur sebagai embun antiseptik. Covid-19 is a cause of respiratory tract infections. The spread of Covid-19 can be minimized in several ways including spraying antiseptic povidone iodine. The purpose of this study is to produce a useful tool in an effort to minimize the spread of Covid-19 with a microcontroller-based automatic misting antiseptic control sistem. This research uses the development research method. This research was carried out at the researcher's home and completed in the integrated laboratory of electrical engineering education at the State University of Manado. The microcontroller-based antiseptic automatic misting control sistem is a sistem built on a povidone iodine antiseptic spraying device. Microcontroller-based automatic antiseptic misting control sistem can be used to minimize the spread of Covid-19. This microcontroller-based antiseptic misting can work in accordance with a sistem designed to spray povidone iodine antiseptic to the human body, the way it works is that when an object is at a predetermined point or distance, the motion sensor will detect it and will send a signal to the microcontroller which will then be processed to the actuator / pump that will flow the liquid or fluid to the 0.2 mm misting nozzle as a fluid channel which is positioned to provide turbidity to the fluid in this case povidone iodine antiseptic liquid to exit through the apareture as an antiseptic dew.

Micro Machined Slit Between Ridge and Groove in Micro Fluid-Channel to Measure Floating Cell Deformability

A micro-slit has been designed to measure deformation of a biological single cell passing through the slit. At the middle part of the flow channel, the slit (7 μm height, 0.4 mm width, and 0.1 mm length) has been made by photolithography technique: the combination of the micro-ridge on the transparent polydimethylsiloxane plate and the micro-groove on the glass plate. C2C12 (mouse myoblast cells) was used in the test. The flow of suspension of cells was controlled by the pressure head between the inlet and the outlet. Deformation of cells passing through the micro slit was observed with an inverted phase-contrast microscope. The experimental results show that a cell deforms to the flat circular disk and passes through the micro-slit. Although smaller cells tend to pass the micro-slit easily, some bigger cells also passed the micro-slit. Deformation ratio is independent of the size of each cell. The designed slit has capability to detect quantitative deformability of a single biological cell.